• Title/Summary/Keyword: biochemical property

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Risk Assessment of Nitrogen Dioxide for Hematological and Biochemical Parameter in Mice (Mice를 이용한 혈액 및 생화학적 특성에 대한 Nitrogen Dioxide의 위해성 평가)

  • Jeong, Seong-Wook;Han, Yong-Soo;Park, Heung-Jai
    • Journal of Environmental Science International
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    • v.16 no.5
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    • pp.665-670
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    • 2007
  • In this study, we evaluated the property change and relationship between hematological and biochemical parameters in exposure group and control group by multiple inhalation exposure, of $NO_2$. In case of leukocyte, change of hematological property increased significantly in statistics (p<0.05) in mice experiment. in case of hematocrit of exposure group, it was of decreased significantly in statistics. the tendency of hemoglobin and hematocrit was similar to all for three case. in case of methmoglobin that of the exposure group in mice experiment was meaning increased(p<0.05). For the $NO_2$ influence in biochemical property, total protein and triglyceride was decreased but not significant in statistics, albumin was decreased in the exposure group in the animal experiments, blood urea nitrogen and creatinine increased significantly in statistics in the animal experiments. uric acid and lactate dehydrogenase in serum increased significantly in the exposure group. We have to evaluate risk assessment for the $NO_2$ of indoor air pollutant in low concentration and long time.

Synthesis of Saccharide Nonionic Biosurfactants from Coconut Oil and Characterization of Their Interfacial Properties (코코넛 오일로부터 유래된 당계 비이온 계면활성제 합성 및 계면 특성 연구)

  • Jo, SeonHui;Lee, YeJin;Park, KiHo;Lim, JongChoo
    • Applied Chemistry for Engineering
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    • v.30 no.4
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    • pp.435-444
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    • 2019
  • In this study, two types of nonionic saccharide biosurfactants, GP-6 and GP-7, were prepared from coconut oil and the structure of resulting products was investigated by FT-IR, $^1H-NMR$ and $^{13}C-NMR$ spectrophotometer. The interfacial properties of GP-6 and GP-7 were found to be excellent from interfacial property measurements such as critical micelle concentration, static and dynamic surface tensions, interfacial tension, emulsification power, wetting property and foam stability. Detergency test evaluated by using a Terg-o-tometer showed moderately good detergency compared to that of conventional surfactants used in detergent formulations. Biodegradability, acute oral toxicity, acute dermal irritation and acute eye irritation tests revealed that both surfactants possess excellent mildness and superior environmental compatibility indicating the potential applicability to detergent products formulations. In particular, GP-6 can be considered as a strong candidate in detergent formulations since it is more surface active, mild and readily biodegradable than GP-7.

Biochemical property identification of 10 strains of Bacillus thuringiensis and 10 strains of Bacillus cereus (7 strains of non-emetic and 3 strains of emetic type) by API test

  • Hong, Yong-Gun;Lee, Jin-Joo;Kwon, Seung-Wook;Kim, Sang-Soon
    • Korean Journal of Food Science and Technology
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    • v.52 no.6
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    • pp.678-684
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    • 2020
  • The objective of this study was to identify the fermentation characteristics of Bacillus thuringiensis and emetic, non-emetic Bacillus cereus using analytical profile index (API) test. Ten strains of B. thuringiensis and 10 strains of B. cereus including 3 strains of emetic type were used at the same concentrations. The differences of fermentation characteristics between the B. thuringiensis and B. cereus was not obvious, but the differences between the non-emetic and emetic B. cereus were distinctive. Seven among 50 substrates were negative for all non-emetic B. cereus strains and positive for all emetic strains, and three substrates among additional 12 substrates had the same tendency. From these differences, 3 emetic B. cereus strains were not indicated as B. cereus by API test. These results indicate that API test is not a suitable method to identify some strains of emetic B. cereus, and the distinctive differences in substrate utilization can be used to improve selective media.

Separation of Nattokinase from Bacillus subtilis Fermentation Broth by Expanded Bed Adsorption with Mixed-mode Adsorbent

  • Lu Miao-Hua;Lin Dong-Qiang;Wu Yuan-Chun;Yun Jun-Xian;Mei Le-He;Yao Shan-Jing
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.10 no.2
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    • pp.128-135
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    • 2005
  • Mixed-mode hydrophobic/ionic matrices exhibit a salt-tolerant property for adsorbing target protein from high-ionic strength feedstock, which allows the application of undiluted feedstock via an expanded bed process. In the present work, a new type of mixed-mode adsorbent designed for expanded bed adsorption, Fastline $PRO^{\circledR}$, was challenged for the capture of nattokinase from the high ionic fermentation broth of Bacillus subtilis. Two important factors, pH and ion concentration, were investigated with regard to the performance of nattokinase ad-sorption. Under initial fermentation broth conditions (pH 6.6 and conductivity of 10 mS/cm) the adsorption capacity of nattokinase with Fastline PRO was high, with a maximum capacity of 5,350 U/mL adsorbent. The elution behaviors were investigated using packed bed adsorption experiments, which demonstrated that the effective desorption of nattokinase could be achieved by effecting a pH of 9.5. The biomass pulse response experiments were carried out in order to evaluate the biomass/adsorbent interactions between Bacillus subtilis cells and Fastline PRO, and to demonstrate a stable expanded bed in the feedstock containing Bacillus subtilis cells. Finally, an EBA process, utilizing mixed-mode Fastline PRO adsorbent, was optimized to capture nattokinase directly from the fermentation broth. The purification factor reached 12.3, thereby demonstrating the advantages of the mixed-mode EBA in enzyme separation.

Incidence of Pasteurella multocida infection in Youngnam swine herds and the biochemical properties of the organisms recovered from pigs with atrophic rhinitis and pneumonic lungs (영남지방 돼지의 Pasteurella multocida 감염상태 및 분리균의 생화학적 특성)

  • Cho, Gil-jae;Kim, Bong-hwan
    • Korean Journal of Veterinary Research
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    • v.29 no.4
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    • pp.479-485
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    • 1989
  • The present study was conducted to investigate the incidence of Pasteurella multocida infection in Youngnam swine herds during the period from March 1988 to February 1989 and some properties of the isolated organisms. P multocida was isolated from 22(43.1%) of 51 growing pigs of 4 to 12 weeks of age and from 8(80.0%) of 10 herds. From nasal turbinates of 102 slaughtered pigs, 47(46.1%) pigs were culture positive and pigs from 8(88.9%) of 9 herds were found to be infected with P multocida. From lungs of 101 slaughtered pigs, 42(41.6%) pigs were culture positive and the pigs from 11(91.7%) of 12 herds were found to be infected with P multocida. The majority of biochemical and cultural properties of the P multocida isolates were identical to those of the standard strains. The isolation frequencies of P multocida in relation to pig snout lesion grades of 0 to 5 were 28.6%, 41.6%, 48.0%, 50.0%, 85.7%, and 100%, respectively.

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Synthesis of Carboxylate-Based Anionic surfactant from Coconut Oil Source and Characterization of Interfacial Properties (코코넛 오일로부터 유래된 카르복실레이트계 음이온 계면활성제의 합성 및 계면 특성에 관한 연구)

  • Lee, Ye Jin;Park, Ki Ho;Shin, Hee Dong;Lim, Jong Choo
    • Applied Chemistry for Engineering
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    • v.32 no.3
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    • pp.260-267
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    • 2021
  • In this study, a carboxylate-based anionic surfactant SLEC-3 was prepared from coconut oil and the structure was elucidated by using FT-IR, 1H-NMR and 13C-NMR analysis. Measurements of interfacial properties such as critical micelle concentration, static and dynamic surface tensions, emulsification index, and foam stability have shown that SLEC-3 is better in terms of interfacial activity and more effective in lowering interfacial free energy than those of SLES, which has been widely used as a conventional anionic surfactant in the detergent industry. Biodegradability, acute oral toxicity and dermal irritation tests also revealed that SLEC-3 surfactant possesses excellent mildness and low toxicity, indicating the potential applicability in detergents and cleaner products formulation.

A Study on the Preparation of Antibacterial Biopolymer Film

  • Cho, Dong-Lyun;Na, Kun;Shin, Eun-Kyung;Kim, Hyun-JIn;Lee, Ki-Young;Go, Jin-Hwan;Choi, Choon-Soon
    • Journal of Microbiology and Biotechnology
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    • v.11 no.2
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    • pp.193-198
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    • 2001
  • Preparation of antibacterial biopolymer film which is suitable for food packaging film was investigated using K-carrageenan as a base material. K-Carrageenan showed good biodegradability and film-forming characteristic but poor mechanical properties under humid condition. Also, various bacteria grew well on its surface. The poor mechanical properties could be improved by mixing with alginate at a 1:1 ratio and crosslinking with $CaCl_2$ solution. Antibacterial property coul be provided by modifying the K-carrageenan film surface with acrylic acid plasma followed by ion-exchange with $Ag^+$ ions. Such prepared film still showed good biodegradability by various fongi.

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Improvement of Functional Properties of Ovotransferrin by Phosphorylation through Dry-heating in the Presence of Pyrophosphate

  • Hayashi, Yoko;Li, Can-Peng;Enomoto, Hirofumi;Ibrahim, Hisham R.;Sugimoto, Yasushi;Aoki, Takayoshi
    • Asian-Australasian Journal of Animal Sciences
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    • v.21 no.4
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    • pp.596-602
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    • 2008
  • Ovotransferrin (OTf) was phosphorylated by dry-heating in the presence of pyrophosphate at pH 4.0 and $85^{\circ}C$ for 1 and 5 d, and the functional properties of phosphorylated OTf (PP-OTf) were investigated. The phosphorus content of OTf increased to 0.91% as a result of phosphorylation and the electrophoretic mobility of PP-OTf also increased. Although the solubility of dry-heated OTf slightly decreased, the decrease was reduced by phosphorylation. The stability against heat-induced insolubilization of OTf was somewhat improved by phosphorylation, but more than 70% of PP-OTf was insolubilized when it was heated at $70^{\circ}C$ for 10 min at pH 7.0. However, heat-induced insolubilization of PP-OTf was reduced when it was heated in the presence of phosphorylated ovalbumin. This may explain the excellent stability of phosphorylated egg white protein against heat-induced insolubilization which was reported previously. The emulsifying property of OTf was also somewhat improved by phosphorylation. The calcium phosphate-solubilizing ability of PP-OTf was enhanced. Although the degree of phosphorylation of OTf by dry-heating in the presence of pyrophosphate was similar to that of ovalbumin, the improvement of properties of PP-OTf was considerably different from those of phosphorylated ovalbumin.

Insecticidal Efficacy of Porteresia coarctata (Roxb.) on Bio-chemical Alteration of Spodoptera litura (Fab.)

  • Bhattacharyya Atanu;Goswami Arunava;Dihidar Shankar;Adhikari Sujit;Banerjee Sudip Kumar;Talapatra Soumendra Nath;Barik Bikas Ranjan;Bhattacharya Subir Kumar;Chandra Asit Kumar
    • International Journal of Industrial Entomology and Biomaterials
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    • v.14 no.1
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    • pp.9-13
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    • 2007
  • Hexane extract of Porteresia coarctata (Roxb.) exhibits a toxic effect on the tissues of Spodoptera litura (F) while fed at the dose of 1000 and 2000 ppm thoroughly mixing with castor leaves (Ricinus communis L) after dissolving in DMSO at late fourth instar whereas only DMSO treated castor leaves were fed to control group. The larvae were put to rear at $28^{\circ}C{\pm}1^{\circ}C$, $76{\pm}4%$ R.H. under 12 L + 12 D photoperiodic regime. In test group insects substantial reduction of protein and DNA content was marked in fat body and midgut tissues compared to DMSO treated control group. The significant biochemical alterations in the midgut tissues and fat body of test group insects indicate the insecticidal property of the said plant extract that could be tested in facilitating the phenomenal stride in Integrated Pest Management.

Fluoroethylene Carbonate Addition Effect on Electrochemical Properties of Mixed Carbonate-based Organic Electrolyte Solution for a Capacitor

  • Kim, Mingyeong;Kim, Ick-Jun;Yang, Sunhye;Kim, Seok
    • Bulletin of the Korean Chemical Society
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    • v.35 no.2
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    • pp.466-470
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    • 2014
  • In this paper, organic solvent electrolytes were prepared by a mixture of propylene carbonate (PC), dimethyl carbonate (DMC), tetraethylammonium tetrafluoroborate ($TEABF_4$)s to evaluate the ionic properties of propylene carbonate (PC)/dimethyl carbonate (DMC) mixtures as solvents for a capacitor application, in view of improving the electrochemical performances. The bulk resistance and interfacial resistance of the mixture electrolytes were investigated using an AC impedance method. The morphology of carbon-based electrodes which were contained in different electrolytes was analyzed by scanning electron microscopy (SEM) method. From the experimental results, by increasing the FEC content, capacitance of electrodes was increased, and the interfacial resistance was decreased. In particular, by a content of 2 vol % FEC in 0.2 M $TEABF_4$ PC/DMC solvent, the electrolyte showed the superior capacitance. However, when FEC content exceeds 2 vol %, the capacitance was decreased and the interfacial resistance was increased.