• Microbiology and Biotechnology Letters
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• v.42 no.4
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• pp.377-385
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• 2014

In this study, a wastewater treatment system for hypersaline wastewater utilizing the Hypersaline Wastewater Treatment Community (HWTC) has been developed. The hypersaline wastewater treatment efficiency and microbial community of the HWTC were investigated. The average removal efficiencies of chemical oxygen demand were 84% in an HRT of 2.5 days. Microbial community analysis, by denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S rRNA gene fragments and 16S rRNA gene clone library, revealed community diversity. The 16S rRNA gene analysis of dominant microbial bacteria in 4% hypersaline wastewater confirmed the presence of Halomonas sp. and Paenibacillus sp. Phylogenetic analysis suggested that the taxonomic affiliation of the dominant species in the HWTC was ${\gamma}$-proteobacteria and firmicutes. These results indicate the possibility that an appropriate hypersaline wastewater treatment system can be designed using acclimated sludge with a halophilic community.

• Korean Journal of Environment and Ecology
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• v.21 no.3
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• pp.235-242
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• 2007

In order to find out the current situation of the fauna of the mammals which inhabit Hallasan National Park, the areas of Seongpanak, Eorimok, Gwaneumsa, Yeongsil and Donnaeko were surveyed. The survey was conducted from April 2006 to January 2007 The analysis of geographical distribution revealed that there are 15 species of mammals, which belong to eight families and five orders, in which Apodemus chejuensis, Carpreolus pygargus tianschanicns, Rhinolophus ferrumequinum and Tamias sibiriricus barberi were found to be dominant species. It was also found that there exists the fauna of mammals in Mt. Halla such as Mustela sibirica quelpartis, Crocidura dsinezmi quelpartis, Micromys minutus hertigi and Sorex caecutiens hallamontanus, showing a bio-geological attribute that territorial population of taxologically geographical subspecies class exists. The fauna of the mammals in Jeju Island is rather simpler in its species composition in comparison with the inland of the Korean Peninsula ever since Jeju Island's geographical isolation from the mainland, mainly consisting of Chiroptera and small rodents.

• Journal of the Korean Institute of Landscape Architecture
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• v.32 no.2
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• pp.86-101
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• 2004

This study was conducted to present the ecological management of Goduk Riverside Restoration Area in Hangang, Seoul by analyzing the change of the vegetation structure. The survey site was classified into three groups. These were the vegetation restoration area, the dry plant area, and the swampy plant area. There were 141 taxa including naturalized plants and 13 species recorded in 2001 and 258 taxa including naturalized plants and 42 species were recorded in 2003 by monitoring. Monitoring results showed that the alien plants such as Humulus japonicus expanded continuously except in the vegetation restoration area. It was found that the growing status of planted shrub plants were poor, and the naturalized plants status was thriving, and the soil environment was bad in the vegetation restoration area. The alien plants such as Humulus japonicus and Aster pilosus dominated continuously in the dry plant area. The swampy native herb plants number was decreased, but the Humulus japonicus community was expanded caused by the soil drying in the swampy plant area. Soil analysis showed that the soil acidity, the available phosphates and the concentration of calcium were highly effected by cultivation. We propose ecological management as follows based on the results of the change of vegetation and soil characteristics. The vegetation restoration area should be managed by visitor's characteristics. Replanting vegetations should be based on soil characteristics. The removal of naturalized plants and established monitoring with plots is also needed. In the dry plant area and the swampy plant area, naturalized plants need to be removed in order to facilitate bio-diversity and monitoring.

• Journal of the Korean association of regional geographers
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• v.8 no.1
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• pp.98-109
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• 2002

The long term land-cover changes and the pattern of morphological changes in foredune ridges and unvegetated dunes were investigated for about 30 years through analysing aerial photographs in Shinduri coastal dunefield, Korea. As a result, forested dune area increased while unvegetated dune area decreased continuously since 1967. Foredune ridges retreated landward about 80m away from the former coast-line in the middle part while they advanced seaward after construction of dike in the northern part during last 3 decades. Unvegetated dunes in the middle part of the dunefield were eroded at seaward side and moved landward away. These facts mean not only coastal dune area has been affected by man-made effects such as afforestation and coastal developments but also shinduri coastal dune area has been stabilized by plants and has been negative sediment budget.

• Journal of Species Research
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• v.7 no.3
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• pp.231-239
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• 2018

While screening indigenous prokaryotic species in Republic of Korea in 2017, a total of 17 bacterial strains assigned to the phylum Bacteroidetes were isolated from a variety of environmental habitats including water of fountain, tidal flat, plant root, soil, the gut of Russian grayling butterfly, ginseng field, seawater, lagoon and seashore sand. From the 16S rRNA gene sequence similarity of more than 98.7% and the formation of a robust phylogenetic clade with the closest species, it was found that the 17 strains belong to independent and recognized bacterial species. There has been no official report that the identified 17 species have been previously isolated in the Republic of Korea. Thus, 15 species in 10 genera of one family in the order Flavobacteriales, one species in one genus of one family in the order Cytophagales, and one species in one genus of one family in the order Sphingobacteriales are proposed as unrecorded species of the phylum Bacteroidetes found in the Republic of Korea. Gram reaction, colony and cell morphology, basic phenotypic characteristics, isolation source, taxonomic status, strain ID and other information are described in the species descriptions.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• v.2 no.3
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• pp.170-179
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• 2021

Hulunbuir steppe, one of the four largest steppes in China, has experienced rapidly progressing desertification partly due to overgrazing by livestock. The objective of this study was to investigate the effectiveness of various vegetation recovery methods, including the selection of unpalatable plants less affected by grazing livestock. To determine livestock grazing preferences at Hulunbuir restoration sites, we used DNA barcoding methods to analyze fecal materials of horses and cattle grazing on four restored plants: two trees (Pinus sylvestris L. var. mongolica and Populus canadensis) and two shrubs (Caragana microphylla and Corethrodendron fruticosum). Neither of the two tree species were detected in livestock feces, whereas both shrub species were detected at lo w frequencies. There were no significant differences in compositions of species consumed by horses and cattle except that Asteraceae species were more often consumed by cattle. Our results showed that the four plants used for restoration may be classified as unpalatable or less palatable species in the Hulunbuir restoration area. Our results may help inform restoration strategies implemented in restoration areas, especially regarding negative effects of livestock grazing during the initial stage of restoration in Hulunbuir.

• Journal of Species Research
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• v.8 no.2
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• pp.176-190
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• 2019

A total of 38 bacterial strains within the classes Bacilli and Deinococci were isolated from various sources in Korea. Samples were collected from animal intestine, urine, soil, tidal flat mud, and kimchi. In the sequence comparison and phylogenetic analysis of 16S rRNA sequences, the 38 isolates were assigned to the classes Bacilli and Deinococci with sequence similarities more than 98.7%. Twenty-four strains and 13 strains were classified the order Bacillales and Lactobacillales in the class Bacilli, respectively. In the order Bacillales, there were nine species in the genus Bacillus, seven species in the genus Paenibacillus, and the remaining eight species in the genera Domibacillus, Halobacillus, Virgibacillus, Lysinibacillus, Paenisporosarcina, Planococcus, Savagea, and Staphylococcus. In the order Lactobacillales, there were four species in the genus Lactobacillus, three species in the genus Leuconostoc, three species in the genus Lactococcus, and the remaining three species in the genera Aerococcus, Enterococcus, and Streptococcus. One species was related to the genus Deinococcus of the order Deinococcales. Most of the isolated strains were Gram-stain-positive, but some were Gram-stain-variable or Gram-stain-negative. Cells were rod or cocci-shaped. Based on the results of 16S rRNA analysis, we report 38 strains as previously unrecorded species to Korea, and the basic characteristics of strains are described herein.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.10a
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• pp.20-20
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• 2019

Sedge family is second largest family in the Korean flora with more than 240 taxa, following Aster family (about 300 taxa). Although high species diversity, a very limited number of taxa has been utilized for ethnobotanical usages. In the study, previous researches on Cyperaceae ethnobotanical information are documented and analyzed for future research directions on the taxa. A total number of seven taxa has been used for medicinal, food, and material purposes: Carex boottiana Hook. & Arn., Cyperus amuricus Maxim., Cyperus esculentus L., Cyperus exaltatus Retz., Cyperus rotundus L., Eleocharis kuroguwai Ohwi, and Bolboschoenus maritimus (L.) Palla. Underground parts such as tubers are used with medicinal purpose, and aboveground portions such as leaves and stems provide materials for various tools and foods for people and animals. They are all native to Korea, except C. esculentus, which is introduced from Mediterranean areas for anti-erosion materials. The ethnobotanical value of C. esculentus has been well recognized from ancient times, for example, starch grains in USA (excavations at the Mashantucket Pequot Reservation) and Tiger nut milk (Horchata de chufa in Spain). Among material sources, C. exaltatus has a long history in the Korean peninsula from Three Kingdoms of Korea (57 BC~668 AD). C. rotundus is distributed widely in Asia, Africa, Europe, and America, and utilized for medicines, food, and materials. In addition, Eleocharis kuroguwai is only one taxon utilized for ethnobotanical uses among fifteen Eleocharis Korean native species. The number of species with ethnobotanical uses is small in sedge family, but the family has long been utilized accompanying long human history. Cyperus papyrus L., the source of papyrus paper (Egypt in the Nile Delta), is also a member of sedge family. Sedge family has contributed to human societies in various aspects but, the cultural, botanical, ecological values of family members have been underestimated.

• Food Science of Animal Resources
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• v.41 no.4
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• pp.701-714
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• 2021

In this study, the microbial community succession and the protein hydrolysis of donkey meat during refrigerated (4℃) storage were investigated. 16S rDNA sequencing method was used to analyze the bacteria community structure and succession in the level of genome. Meanwhile, the volatile base nitrogen (TVB-N) was measured to evaluate the degradation level of protein. After sorting out the sequencing results, 1,274,604 clean data were obtained, which were clustered into 2,064 into operational taxonomic units (OTUs), annotated to 32 phyla and 527 genus. With the prolonging of storage time, the composition of microorganism changed greatly. At the same time, the diversity and richness of microorganism decreased and then increased. During the whole storage period, Proteobacteria was the dominant phyla, and the Photobacterium, Pseudompnas, and Acinetobacter were the dominant genus. According to correlation analysis, it was found that the abundance of these dominant bacteria was significantly positively correlated with the variation of TVB-N. And Pseudomonas might play an important role in the production of TVB-N during refrigerated storage of donkey meat. The predicted metabolic pathways, based on PICRUSt analysis, indicated that amino metabolism in refrigerated donkey meat was the main metabolic pathways. This study provides insight into the process involved in refrigerated donkey meat spoilage, which provides a foundation for the development of antibacterial preservative for donkey meat.

• The Korea Journal of Herbology
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• v.34 no.6
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• pp.1-23
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• 2019

Objectives : The purpose of this study was to document the use of medicinal plants in traditional practices and to analyze and evaluate medicinal traditional knowledge of indigenous people in Wolchulsan National Park. Methods : Data were collected through interviews, informal meetings, open and group discussions, and observations guided by semi-structured questionnaires. Data were analyzed via quantitative analysis of use value (UV), informant consensus factor (ICF) and fidelity level (FL), and network analysis. Results : A total of 580 methods of usage recorded in this study were classified into 55 families, 95 genera, and 104 species. Plants with the highest recorded UVs were Glycine max (L.) Merr., Leonurus japonicus Houtt., and Artemisia princeps Pamp.. The informant consensus factor about using medicinal plants ranged from 0.55 to 0.92, which showed a high level of agreement among the informants on respiratory system disorders and pains. There were 22 species of plants with a fidelity level of 100 %, after eliminating the plants that were mentioned only once from the analysis. Finally, using network analysis, Glycine max (L.) Merr. and Artemisia princeps Pamp. were defined as species with meaningful medicinal use, while lumbago and leg pain were defined as significant ailments in the study area. Conclusions : This study highlights the diversity and importance of medicinal traditional knowledge for communities of Wolchulsan National Park, Korea. The results of this study will provide basic data for phytochemical and pharmaceutical studies, such as new medicines and therapies.