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http://dx.doi.org/10.4014/kjmb.1410.10001

Microbial Community Analysis in the Wastewater Treatment of Hypersaline-Wastewater  

Lee, Jae-Won (Department of Environmental Engineering, Korea Maritime and Ocean University)
Kim, Byung-Hyuk (Department of Environmental Engineering, Korea Maritime and Ocean University)
Park, Yong-Seok (Q-BioTech Corporation)
Song, Young-Chae (Department of Environmental Engineering, Korea Maritime and Ocean University)
Koh, Sung-Cheol (Department of Environmental Engineering, Korea Maritime and Ocean University)
Publication Information
Microbiology and Biotechnology Letters / v.42, no.4, 2014 , pp. 377-385 More about this Journal
Abstract
In this study, a wastewater treatment system for hypersaline wastewater utilizing the Hypersaline Wastewater Treatment Community (HWTC) has been developed. The hypersaline wastewater treatment efficiency and microbial community of the HWTC were investigated. The average removal efficiencies of chemical oxygen demand were 84% in an HRT of 2.5 days. Microbial community analysis, by denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S rRNA gene fragments and 16S rRNA gene clone library, revealed community diversity. The 16S rRNA gene analysis of dominant microbial bacteria in 4% hypersaline wastewater confirmed the presence of Halomonas sp. and Paenibacillus sp. Phylogenetic analysis suggested that the taxonomic affiliation of the dominant species in the HWTC was ${\gamma}$-proteobacteria and firmicutes. These results indicate the possibility that an appropriate hypersaline wastewater treatment system can be designed using acclimated sludge with a halophilic community.
Keywords
Wastewater treatment; hypersalinity treatment; microbial community;
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1 Abou-Elela SI, Kamel MM, Fawzy ME. 2010. Biological treatment of saline wastewater using a salt-tolerant microorganism. Desalination. 250: 1-5.   DOI
2 APHA, AWWA, WEF. 2005. Standard methods for the examination of water and wastewater. APHA, Washington, DC, USA.
3 Burnett WE. 1974. The effect of salinity variations on the activated sludge process. Water Sew. Works. 121: 37-38.
4 Dastgheib SMM, Amoozegar MA, Khajeh K, Ventosa A. 2011. A halotolerant Alcanivorax sp. strain with potential application in saline soil remediation. Appl. Microbiol. Biotechnol. 90: 305-312.   DOI
5 de Albuquerque JP, Keim CN, Lins U. 2010. Comparative analysis of Beggiatoa from hypersaline and marine environments. Micron. 41: 507-517.   DOI
6 Ishii K, Fukui M. 2001. Optimization of annealing temperature to reduce bias caused by a primer mismatch in multitemplate PCR. Appl. Environ. Microbiol. 67: 3753-3755.   DOI   ScienceOn
7 Jaspers E, Nauhaus K, Cypionka H, Overmann J. 2001. Multitude and temporal variability of ecological niches as indicated by the diversity of cultivated bacterioplankton. FEMS Microbiol. Ecol. 36: 153-164.   DOI
8 Jin HJ, Tu R, Xu F, Chen SF. 2011. Identification of nitrogenfixing Paenibacillus from different plant rhizospheres and a novel nifH gene detected in the P. stellifer. Microbiology 80: 117-124.   DOI
9 Jung RK, Yang HC, Lee JJ, Kim YH. 2008. A novel halophilic Halomonas sp. having an excellent digestive effect of organic material, a soil conditioner for the reclaimed land using said strain and a method for plant growth-promoting thereof. Republic of Korea patent application.
10 Kargi F, Dincer AR. 1998. Saline wastewater treatment by halophile-supplemented activated sludge culture in an aerated rotating biodisc contactor. Enzyme Microb. Tech. 22: 427-433.   DOI
11 Kargi F, Dincer AR. 1999. Salt inhibition of nitrification and denitrification in saline wastewater. Environ. Technol. 20: 1147-1153.   DOI
12 Kim JS, Pi YT. 2010. Oceanobacillus sp. strain having excellent salt-resistance and use thereof. Republic of Korea patent application.
13 Kincannon DF, Gaudy AF. 1966. Some effect of high salt concentration on activated sludge. J. Water Poll. Cont. Fed. 38: 1148-1158.
14 Kincannon DF, Gaudy AF. 1968. Response of biological waste treatment system to changes in salt concentrations. Biotechnol. Bioeng. 10: 483-496.   DOI
15 Liebgott P-P, Labat M, Casalot L, Amouric A, Lorquin J. 2007. Bioconversion of tyrosol into hydroxytyrosol and 3,4-dihydroxyphenylacetic acid under hypersaline conditions by the new Halomonas sp. strain HTB24. FEMS Microbiol. Lett. 276: 23-33.
16 Kinner NE, Bishop PL, Asce M. 1962. Treatment of saline domestic wastewater using RBC's. J. Environ. Eng. ASCE. 108: 650-663.
17 Kleinsteuber S, Riis V, Fetzer I, Harms H, Muller S. 2006. Population dynamics within a microbial consortium during growth on diesel fuel in saline environments. Appl. Environ. Microbiol. 72: 3531-3542.   DOI
18 Lefebvre O, Vasudevan N, Thanasekaran K, Moletta R, Godon JJ. 2006. Microbial diversity in hypersaline wastewater: the example of tanneries. Extremophiles 10: 505-513.   DOI
19 Linaric M, Markic M, Sipos L. 2013. High salinity wastewater treatment. Water Sci. Technol. 68: 1400-1405.   DOI
20 Ludzack FJ, Noran DK. 1965. Tolerance of high salinities by conventional wastewater treatment processes. J. Water Poll. Cont. Fed. 37: 1404-1413.
21 Muyzer G. 1999. DGGE/TGGE a method for identifying genes from natural ecosystems. Curr. Opin. Microbiol. 2: 317-322.   DOI   ScienceOn
22 Oren A, Gurevich P, Malkit A, Heins Y. 1992. Microbial degradation of pollutants at high salt concentrations. Biodegradation. 3: 387-398.   DOI   ScienceOn
23 Raud M, Tutt M, Jogi E, Kikas T. 2012. BOD biosensors for pulp and paper industry wastewater analysis. Environ. Sci. Pollun. Res. 19: 3039-3045.   DOI
24 Surakasi VP, Antony CP, Sharma S, Patole MS, Shouche YS. 2010. Temporal bacterial diversity and detection of putative methanotrophs in surface mats of Lonar crater lake. J. Basic Microbiol. 50: 465-474.   DOI
25 Saratale RG, Saratale GD, Chang JS, Govindwar SP. 2010. Decolorization and biodegradation of reactive dyes and dye wastewater by a developed bacterial consortium. Biodegradation. 21: 999-1015.   DOI
26 Sharghi EA, Bonakdarpour B, Roustazade P, Amoozegar MA, Rabbani AR. 2013. The biological treatment of high salinity synthetic oilfield produced water in a submerged membrane bioreactor using a halophilic bacterial consortium. J. Chem. Technol. Biotechnol. In press.
27 Stewart MJ, Ludwig HF, Kearns WH. 1962. Effect of varying salinity on the extended aeration process. J. Water Poll. Cont. Fed. 34: 1161-1177.
28 Tang J, Zheng A-P, Bromfield ESP, Zhu J, Li S-C, Wang S-Q, et al. 2011. 16S rRNA gene sequence analysis of halophilic and halotolerant bacteria isolated from a hypersaline pond in Sichuan, China. Ann. Microbiol. 61: 375-381.   DOI
29 Tiquia SM, Davis D, Hadid H, Kasparian S, Ismail M, Ahly S, et al. 2007. Halophilic and halotolerant cacteria from river waters and shallow groundwater along the rouge river of Southeastern Michigan. Environ. Technol. 28: 297-307.   DOI
30 Woolard CR, Irvine RL. 1994. Biological treatment of hypersaline wastewater by a biofilm of halophilic bacteria. Water Environ. Res. 66: 230-235.   DOI
31 Woolard CR, Irvine RL. 1995. Treatment of hypersaline wastewater in the sequencing batch reactor. Water Res. 29: 1159-1168.   DOI
32 Muyzer G, Waal ECD, Uitterlinden AG. 1993. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl. Environ. Microbiol. 59: 695-700.
33 Yoshie S, Noda N, Miyano T, Tsuneda S, Hirata A, Inamori Y. 2001. Microbial community analysis in the denitrification process of saline-wastewater by denaturing gradient gel electrophoresis of PCR-amplified 16S rDNA and the cultivation method. J. Biosci. Bioeng. 92: 346-353.   DOI
34 Yoshie S, Noda N, Tsuneda S, Hirata A, Inamori Y. 2004. Salinity decreases nitrite reductase gene diversity in denitrifying bacteria of wastewater treatment systems. Appl. Environ. Microbiol. 70: 3152-3517.   DOI   ScienceOn
35 Kim B-H, Baek K-H, Cho D-H, Sung Y, Ahn C-Y, Oh H-M, et al. 2009. Analysis of microbial community during the anaerobic dechlorination of tetrachloroethylene (PCE) in stream of Gimpo and Inchon areas. Korean J. Microbiol. 45: 140-147.