• 한국농공학회논문집
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• 제56권6호
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• pp.169-176
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• 2014

To evaluate bio-cementation of microbial on sands, laboratory test was conducted using acrylic cubic molding boxes ($5cm{\times}5cm{\times}5cm$). It was incubated the microbial, called Bacillus Pasteurii, according to Park et al (2011, 2012). and applied 50ml each specimen. Two type of sand samples used were Jumoonjin sand and common sand (well graded). These sands were molded in acrylic boxes with the relative density of 30 % and 60 % respectively. Microbial were poured onto the samples molded in acrylic boxes and cured at the room temperature and humidity. After 7, 14 and 21days, it was measured the compressive strength, pH, EC, and density and it were observed SEM and XRD to verify the effect of bio-cementation. It was found that bio-cementation was increased a strength of sands and it was appeared that strengths were related to the type of sand and relative density. Therefore it was confirmed the solidification of sands using the bio-cementation by microbial activation and the usefullness of acrylic molding boxes when tests were conducted on the soil of sands.

• International Journal of Railway
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• 제6권3호
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• pp.90-94
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• 2013

Cement or lime is generally used to improve the strength of soil. However, bacteria were utilized to produce cementation of loose soils in this study. The microo rganism called Bacillus, and $CaCl_2$ was introduced into loose sand and soft silt and $CaCO_3$ in the voids of soil particles were produced, leading to cementation of soil particles. In this study, loose sand and soft silt typically encountered in Korea were bio-treated with 3 types of bacteria concentration. The cementation (or calcite precipitation) in the soil particles induced by the high concentration bacteria treatment was investigated at 7 days after curing. Based on the results of Scanning Electron Microscope (SEM) tests and EDX analyses, high concentration bacteria treatment for loose sand was observed to produce noticeable amount of $CaCO_3$, implying a significant cementation of soil particles. It was observed that higher calcium carbonate depositions were observed in poorly graded distribution as compared to well graded distribution. In addition, effectiveness of biogrouting has also been found to be feasible by bio-treatment without any cementing agent.

• 구강회복응용과학지
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• 제32권3호
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• pp.240-245
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• 2016

치은 연하 변연을 갖는 임플란트 수복물 장착시 잔여 합착제의 제거는 임플란트 생물학적 합병증을 예방하기 위해 매우 중요하다. 치은 연하 잔여 합착제를 최소화하기 위해 인상용 실리콘이나 자가중합형 레진을 이용하여 복제 지대주를 제작하는 여러 방법이 소개되었다. 본 증례에서는 열가소성 접착제(hot melt adhesive material)를 이용하여 복제 임플란트 지대주(abutment replica)제작하는 방법을 소개하고자 한다. 이 방법은 간단하고 부가적인 기공과정이 필요 없어 진료실에서 쉽게 이용할 수 있어 이에 보고하는 바이다.

• 한국산학기술학회논문지
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• 제16권6호
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• pp.4276-4283
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• 2015

본 연구에서는 미생물 반응으로 생성된 탄산칼슘을 현장에서 활용되고 있는 주입장비와 유사하게 제작하여 모래지반에 주입함으로 지반조건에 따른 주입범위 및 주입효과를 확인하고자 하였다. 본 연구를 위해 2종류의 단층토(SP, SW)를 D 150mm ${\times}$ H 300mm의 모형토조에 상대다짐도 80, 85, 90, 95%로 제작하여 주입실험을 수행하였다. 주입 후 강도개선효과를 확인하기 위해 일축압축강도를 측정하였고, 주입노즐 주변의 구근형성을 통해 주입범위를 살펴보았다. 그 결과, SW시료는 상대다짐도 85% 이상에서 주입률이 10% 이하로 나타났으며, 지반의 상대다짐도에 따라 Bio-grouting의 주입률 및 고결범위에 영향이 있음을 확인 할 수 있었다.

• 지질공학
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• 제29권4호
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• pp.383-391
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• 2019

Bio-grouting based on microbial-induced calcite precipitation (MICP) is recently emerging as a novel and environmentally friendly technique for improvement of coarse-grained ground. To date, the mechanical behaviour of bio-grouted coarse-grained soil with different calcite contents and grain sizes still remains poorly understood. The primary objective of this study is to investigate the influence of calcite content on the mechanical properties of bio-grouted coarse-grained soil with different grain sizes. This is achieved through an integrated study of uniaxial loading experiments of bio-grouted coarse-grained soil, 3D digitization of the grains in conjunction with discrete element modelling (DEM). In the DEM model, aggregates were represented by clump logic based on the 3D morphology digitization of the typical coarse-grained aggregates while the CaCO₃ was represented by small-sized bonded particle model. The computed stress-strain relations and failure patterns of the bio-grouted coarse-grained soil were validated against the measured results. Both experimental and numerical investigation suggest that aggregate sizes and calcite content significantly influence the mechanical behaviour of bio-cemented aggregates. The strength of the bio-grouted coarse-grained soil increases linearly with calcite content, but decreases non-linearly with the increasing particle size for all calcite contents. The experimental-based DEM approach developed in this study also offers an optional avenue for the exploring of micro-mechanisms contributing to the mechanical response of bio-grouted coarse-grained soils.

• Geomechanics and Engineering
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• 제17권5호
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• pp.429-438
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• 2019

Microbially induced carbonate precipitation (MICP) is an innovative soil improvement approach utilizing metabolic activity of microbes to hydrolyze urea. In this paper, the shear response and the erodibility of MICP-treated sand under axial compression and submerged impinging jet were evaluated at a low confining stress range. Loose, poorly graded silica sand was used in testing. Specimens were cemented at low confining stresses until target shear wave velocities were achieved. Results indicated that the erodibility parameters of cemented specimens showed an increase in the critical shear stress by up to three orders of magnitude, while the erodibility coefficient decreased by up to four orders of magnitude. Such a trend was observed to be dependent on the level of cementation. The treated sand showed dilative behavior while the untreated sands showed contractive behavior. The shear modulus as a function of strain level, based on monitored shear wave velocity, indicated mineral debonding may commence at 0.05% axial strain. The peak strength was enhanced in terms of emerging cohesion parameter based on utilizing the Mohr-Coulomb failure criteria.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2004년도 춘계학술발표회
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• pp.203-210
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• 2004

Consolidation properties were analysed by means of depositional environments. Depositional environments including geochemical properties, porewater chemistry, sediment structures, particle size distributions and carbon age dating were analysed using undisturbed samples retrieved successively from a boring hole in the study area. Laboratory oedometer tests and anisotropic consolidated triaxial tests(CKoUC) were performed to examine the overconsolidation phenomenons. Based on the carbon age dating results and profiles of geochemical properties, porewater chemistry, salinity and pH, it was founded that the upper silt/clay complex layer was deposited under marine condition while sand and clay layers were deposited under fluvial condition. Planar laminated structures of silts and clays were dominant in marine deposits. Although there was no clear evidences that geological erosion had been occurred in marine deposits, overconsolidation ratio obtained from oedometer tests were greater than unity. Stress paths of samples behaved similar to those of normally consolidated clays. Data plotted in stress state charts proposed by Burland(1990) and Chandler(2000) showed that the marine deposits were geologically normally consolidated. These apparent overconsolidations can be explained by the fabric and chemical bonding due to the difference of the rate of deposition.

• 한국철도학회논문집
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• 제16권6호
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• pp.473-481
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• 2013

본 연구에서는 미생물 반응으로 생성된 탄산칼슘을 현장조건과 유사한 장비로 제작하여 모래지반에 주입함으로 모래지반의 강도 개선 및 주입범위를 평가하고자 하였다. 본 연구를 분석하기 위해 단층토(Sand, SP, SW)는 D 150mm ${\times}$ H 200mm로 3가지 case를 제작하였고, 다층토(SW/SP, SP/SW)는 D 150mm ${\times}$ H 300mm로 제작하여 2가지 case를 Dr 30%인 연약지반으로 제작하여 1일 동안 주입실험을 수행하였다. 포켓관입시험기로 일축압축강도를 측정하였고, 주입노즐 주변의 구근형성을 통해 주입범위를 살펴보았다. 또한, XRD분석 통해 공시체내의 성분분석을 진행하였다. 그 결과, 다층토에서 SP/SW에서 전구간에 걸쳐 약 500kPa의 강도와 15cm 두께의 고결을 확인하였다. 이는 바이오그라우팅 기술이 지반의 간극 상태와 크기에 따라 주입 효과에 상당한 영향이 있음을 확인 할 수 있었다.

• 한국지반공학회논문집
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• 제32권8호
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• pp.27-33
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• 2016

본 연구에서는 미생물 고결토의 압축강도 및 인장강도 개선을 위하여 PVA(Polyvinyl alcohol) 섬유를 혼합하는 연구를 수행하였다. 미생물 고결토의 인장강도 특성을 개선하기 위해 모래에 섬유를 혼합한 다음 미생물 배양액을 7일 동안 1일 2회 총14회 반복주입하여 고결을 유도하였다. 모래는 Ottawa모래를 사용하였으며, 섬유는 PVA섬유를 세 종류의 함유량(0, 0.4, 0.8%)으로 혼합하였다. 미생물은 Bacillus sp. 미생물을 사용하였으며, 공시체의 크기는 직경 5cm, 높이 10cm로 제작하였다. 고결이 완료된 다음 일축압축강도, 인장강도시험을 실시하였으며, 시험 후에는 탄산칼슘 석출량과 SEM 분석을 실시하였다. 섬유의 함유량이 증가함에 따라 평균 일축압축강도는 증가하다가 약간 감소하는 경향을 보이지만, 인장강도는 점진적으로 증가하는 경향을 보였다. 탄산칼슘 석출량이 유사하다고 볼 경우, 압축강도는 약 30%의 강도 증가를 발생하였지만, 인장강도는 약 160%의 강도 증가를 보였다. 공시체의 취성도를 나타내는 압축강도와 인장강도의 비는 섬유 함유량이 0%인 경우 약 8 정도에서 섬유 함유량이 0.8%로 증가할 경우 4까지 감소하였으며, 동일한 조건에서 섬유의 함유량이 증가할수록 인장강도의 증가 폭은 커짐을 확인하였다. 이러한 섬유를 혼합한 미생물 고결토는 전단파괴 방지 및 인장강도 증진을 요하는 사면 등의 분야에 적용 가능 할 것으로 판단된다.