• 제목/요약/키워드: bio-adsorption

검색결과 153건 처리시간 0.023초

표면 미세 가공기술을 이용한 마이크로 캔틸레버의 제작과 바이오센서로의 응용 (Fabrication of Microcantilever-based Biosensor Using the Surface Micromachining Technique)

  • 유경아;정승룡;강치중;김용상
    • 대한전기학회논문지:전기물성ㆍ응용부문C
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    • 제55권1호
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    • pp.11-15
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    • 2006
  • We propose an optical and an electrical detection methods for detecting various bio-molecules effectively with microcantilevers. The microcantilevers were fabricated employing surface micromachining technique that has attractive advantages in terms of cost efficiency, simplicity and ability of fabricating in array. The fluid cell system for injection of bio-molecular solution is fabricated using polydimethylsiloxane (PDMS) and a fused silica glass. The microcantilever is deflected with respect to the difference of the surface stress caused by the formation of self-assembled bio-molecules on the gold coated side of the microcantilever. It detected cystamine dihydrochloride and glutaraldehyde molecules and analyzed individual concentrations of the cystamine dihydrochloride solution. We confirm that the deflections of bending-up or bending-down are occurred by the bio-molecule adsorption and microcantilever can be widely used to a ${\mu}-TAS$ and a lab-on-a-chip for a potential detection of various bio-molecules.

완속여과공정에서 운전시간 및 여층깊이에 따른 자연유기물질(NOM) 제거 특성 (Removal characteristics of NOMs in a slow sand filter at different media depth and operation time)

  • 박노백;박상민;서태경;전항배
    • 상하수도학회지
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    • 제22권4호
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    • pp.467-473
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    • 2008
  • Natural organic matter (NOM) removal by physico-chemical adsorption and biological oxidation was investigated in five slow sand filters with different media depths. Non-purgeable dissolved organic carbon(NPDOC) and $UV_{254}$ absorbance were measured to evaluate the characteristics of NOM removal at different filter depths. Removal efficiency of NOM was in the range of 10-40% throughout the operation time. At start-up of the filters packed with clean sand media, NOM was probably removed by physico-chemical adsorption on the surface of sand through the overall layer of filter bed. However, when Schumutzdecke layer was built up after 30 days operation, the major portion of NPDOC was removed by biological oxidation and/or bio-sorption in lower depth above 50 mm. NOM removal rate in the upper 50 mm filter bed was $0.82hr^{-1}$. It was about 20 times of the rate($0.04hr^{-1}$) in the deeper filter bed. Small portion of NPDOC could be removed in the deeper filter bed by both bio-sorption and biodegradation. SEM analysis and VSS measurement clearly showed the growth of biofilm in the deeper filter bed below 500 mm, which possibly played an important role in the NOM removal by biological activity besides the physco-chemical adsorption mechanism

Gel Electrophoresis Analysis of the Hard Coronas of Human Serum Albumin on Silica Nanoparticles: Size Dependence of Corona Formation

  • Kim, Sung-Jong;Han, Sang Yun
    • Bulletin of the Korean Chemical Society
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    • 제35권9호
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    • pp.2621-2624
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    • 2014
  • The rapid and spontaneous adsorption of proteins on nanoparticle (NP) surfaces in biological fluids such as blood is an important phenomenon as it possibly determines "what the cells see" and, thus, the fates of NPs in living organisms. In order to quantitatively understand protein coronas at the molecular level, we investigated human serum albumin (HSA) coronas that were produced on silica NPs of 20 nm and 50 nm diameters using conventional gel electrophoresis. Analysis of the concentration dependence of protein adsorption showed that HSA coronas preferentially formed a monolayer on silica NPs and revealed the presence of hard protein coronas. HSA adsorption was clearly dependent on NP size, and this might be due to the different surface curvatures of NPs of different sizes.

생물흡착제의 고정화 방법에 대한 고찰 (Review for Immobilization Methods of Biosorbent)

  • 전충
    • 유기물자원화
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    • 제19권2호
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    • pp.16-21
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    • 2011
  • 생물흡착제는 그 자체만으로 실제폐수처리에 적용하기에는 충분하지 못한 구조를 가지고 있기 때문에 고정화는 매우 중요하다. 그래서 최근의 연구는 생물흡착제를 효과적으로 고정화시키는 방법에 초점이 맞추어져 있다. 미생물이나 다당류와 같은 생물흡착제의 기계적 강도를 향상시키기 위하여 지금까지 많은 고정화 방법이 제안되어 왔다. 본 연구에서는 다양한 고정화 방법들(adsorption, covalent binding, entrapment, encapsulation, and crosslinking)을 소개하고자 한다.

식용 제비집으로부터 비극성 비드기술을 활용한 시알산의 분리정제방법에 관한 연구 (A Study on Purification Process of Sialic Acid from Edible Bird's Nest Using Affinity Bead Technology)

  • 김동명;정주영;이형곤;권용성;백진홍;한인석
    • 한국해양바이오학회지
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    • 제12권2호
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    • pp.81-90
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    • 2020
  • Sialic acid, which is contained in about 60-160 mg/kg in the edible bird's nest (EBN), is known to facilitate in the proper formation of synapses and improve memory function. The objective of this study is to extract effectively the sialic acid from edible bird's nest using affinity bead technology (ABT). After preparing the non-polar polymeric bead "KJM-278-28A" having a porous network structure, and then desorbed sialic acid was concentrated and dried. The analysis of the physicochemical properties of bead "KJM-278-28A" showed that the particle size was 400-700 ㎛, the moisture holding capacity was 67-70%, the surface area (BET) was 705-900 ㎡/g, and the average pore diameter 70-87 Å. The adsorption capacity of the bead "KJM-278-28A" for sialic acid was shown a strong physical force to bind sialic acid to the bead surface of 400 mg/L. In addition, as a result of analyzing the adsorption and desorption effects of sialic acid on water, ethanol, and 10% ethanol on the bead, it was confirmed that desorption effectively occurs from the beads when only ethanol is used. As a result of HPLC measurement of the separated sialic acid solution, a total of four sialic acid peaks of N-acetyl-neuraminic acid (Neu5Ac), α,β-anomer of Neu5Ac and N-glycoly-neuraminic acid were identified. Through these results, it was confirmed that it is possible to separate sialic acid from EBN extract with efficient and high yield when using ABT.

Adsorption Characteristics of Cd, Cu, Pb and Zn from Aqueous Solutions onto Reed Biochar

  • Choi, Ik-Won;Kim, Jae-Hoon;Lee, Soo-Hyung;Lee, Jae-Kwan;Seo, Dong-Cheol;Cho, Ju-Sik
    • 한국토양비료학회지
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    • 제49권5호
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    • pp.489-494
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    • 2016
  • Carbon-based sorbents such as biochar and activated carbon have been proven to be cost-effective in removing pollutants containing heavy metals from wastewater. The aim of this study was using batch experiment to evaluate the adsorption characteristics of heavy metals in single-metal conditions onto reed biochar for treating wastewater containing heavy metals. The removal rates of heavy metals were in the order of Pb > $Cu{\fallingdotseq}Cd{\fallingdotseq}Zn$, showing the adsorption efficiency of Pb was higher than the other heavy metals. Freundlich and Langmuir adsorption isotherms were used to model the equilibrium adsorption data obtained from adsorption of Pb on reed biochar. For reed biochar, the Langmuir model provided a slightly better fit than the Freundlich model. Lead was observed on the biochar surface after adsorption by scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS). The main functional groups of reed biochar were aromatic carbons. Overall, the results suggested that reed biochar could be useful adsorbent for treating wastewater containing Pb.

Design Effect of Different Components and Economic Evaluation of an Adsorption Chiller on the System Performance

  • Bidyut B. Saha;Shigeru Koyama;K.C. Amanul Alam;Lee, Jong-Boong
    • 한국공작기계학회:학술대회논문집
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    • 한국공작기계학회 2002년도 춘계학술대회 논문집
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    • pp.17-22
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    • 2002
  • A conventional silica gel/water adsorption chiller has been analyzed numerically. A novel non-dimensional mathematical model has been presented to analyze the design effect of different components of an adsorption chiller. The design parameters of this system are characterized by the number of transfer unit, NTU, of different components and the inert material alpha number, ${\alpha}$of different components of the systems. Results show that condenser NTU$\sub$a/ has the most influential effect on the system performance, which is fellowed by absorber NTU$\sub$e/. It is also seen that coefficient of performance (COP) and non-dimensional specific cooling capacity increases with the increase of NTU$\sub$a/ and NTU$\sub$e/, but decreases with the increase of inert material alpha number. A thermo-economic data of the adsorption chiller and some other heat pump systems those are in practical operation are also presented.

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화산력-바이오 블록의 식생 및 수질정화 특성 (Vegetation and Water Purification Characteristics of Lapilli-Bio Block)

  • 박성용;남부현;김정면;최기봉;연규석;김용성
    • 한국농공학회논문집
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    • 제58권6호
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    • pp.47-54
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    • 2016
  • This study is a basic research on the functional performance review for the use of environmentally friendly construction materials as lapilli-bio block. The neutralization processed Mt. Backdusan and Mt. Hallasan lapilli-bio block were identified vegetation characteristics and water purification properties. As a result, the adsorption of root was more favorable, depending on the material properties of many voids Lapilli. And if the neutralization processed lapilli-bio blocks maintain a long-term immersion conditions in contaminated water was found to be expected from the improvement effect of SS, T-N and T-P. However, field trials and continuous studies are expected to be identified by the use of lapilli-bio block.

Membrane fouling control in low pressure membranes: A review on pretreatment techniques for fouling abatement

  • Arhin, Samuel Gyebi;Banadda, Noble;Komakech, Allan John;Kabenge, Isa;Wanyama, Joshua
    • Environmental Engineering Research
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    • 제21권2호
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    • pp.109-120
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    • 2016
  • Conventional treatment techniques cannot meet the stringent modern water quality regulations emanating from the need to provide high quality drinking water. Therefore, a number of studies have suggested low pressure membrane filtration as a worthwhile alternative. However, a major constraint to the extensive use of this technology in low and middle income countries is the high operating and maintenance costs caused by the inherent predisposition to membrane fouling. Notwithstanding, pretreatment of feed water using techniques such as coagulation, adsorption, oxidation and bio-filtration is believed to control fouling. In this review paper, the existing scientific knowledge on membrane fouling and pretreatment techniques for controlling fouling in low pressure membranes is analyzed with the aim of providing new and valuable insights into such techniques, as well as unveiling crucial issues noteworthy for further studies. Among the techniques reviewed, coagulation was observed to be the most cost-effective and will remain the most dominant in the coming years. Although oxidants and magnetic ion exchange resins can also control fouling, the propensity of oxidants to form health treating precursors and the high economic implications of magnetic ion exchange resins will hinder their adoption in developing countries.