• Biomedical Science Letters
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• v.24 no.4
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• pp.435-438
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• 2018

Acanthamoeba culbertsoni is the causative agent of granulomatous amoebic encephalitis (GAE), a condition that predominantly occurs in immunocompromised individuals and which is typically fatal. A mannose-binding protein (MBP) among lectins was shown to have strong A. castellanii pathogenic potential when correlated with major virulence proteins. In this study, protective effects were analyzed using the monoclonal antibody to A. culbertsoni MBP by quantification and were also compared with other free-living amoebae. For the amoebial cytotoxicity to the target cell, amoeba trophozoites were incubated with Chinese hamster ovary (CHO) cells. For the protective effects of antibodies, amoebae were pre-incubated with them for 4 h and then added to the target cells. After 24 h, the supernatants were collected and examined for host cell cytotoxicity by measuring lactate dehydrogenase (LDH) release. The cytotoxicity of A. culbertsoni to the CHO cells showed about 87.4%. When the monoclonal antibody was pre-incubated with A. culbertsoni, the amoebial cytotoxicity was remarkably decreased as shown at LDH release (1.858 absorbance), which was represented with about 49.9%. Taken together, it suggested that the monoclonal antibody against MBP be important to inhibit the cytotoxicity of A. culbertsoni trophozoites to the target cell. The antibody will be applied into an in vivo functional analysis, which would help to develop therapeutics.

• YAKHAK HOEJI
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• v.45 no.3
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• pp.302-309
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• 2001

Wheat germ agglutinin (WGA) pectin, which binds to human melanoma cell line, was conjugated with Praecoxin A using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide as a cross-linking agent. Physical mixture (PM) of WGA and Praecoxin A was also prepared by using a non-specific binding property of Praecoxin A to WGA. The WGA:Praecoxin A ratio in the conjugate and PM was approximately 1:18 and 1:20, respectively. The results of hemagglutination assay and enzyme-linked lectin assay indicated that the conjugate and PM maintained the lectin-like properties of the WGA. The binding ratio of conjugate was about 70% during 4-24 hr, but the most of Praecoxin A was released within 24 hr in the case of PM. These results lead to the conclusion that the conjugate is potentially useful for the formulation of injection that requires targeting for melanoma as well as sustained release at the site.

• Proceedings of the Korean Information Science Society Conference
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• 2003.04d
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• pp.448-450
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• 2003

인터넷 프로토콜의 이동성 지원(Mobile IP)은 단말의 이동성(host mobility)뿐만 아니라 네트워크의 이동성(network mobility)까지도 지원하도록 확장되고 있다. 그러나 이동 네트워크(mobile network)의 내부에 이동 단말(mobile host) 또는 이동 네트워크가 존재하는 경우와 칼이 중첩된 이동 네트워크(nested mobile network)의 경우 데이터가 전송되는 경로가 삼각형 또는 그 이상으로 복잡해지는 Triangular Routing 또는 Pinball Routing 문제가 발생하게 된다. 본 논문에서는 대응 단말(Correspondent node) 또는 Home Agent에서 위치 정보가 갱신(Binding Update)되는 과정을 재귀적으로 수행하게 함으로써 중첩된 이동 네트워크에서 최적의 경로로 데이터를 전달할 수 있도록 하는 방법을 제시한다.

• YAKHAK HOEJI
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• v.52 no.3
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• pp.225-231
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• 2008

To clarify effects of the structural changes of Fur protein on the resistance to metronidazole (Mtz), the mutational analysis of structure and function of the protein in Helicobacter pylori (Hp) was undertaken. It was identified that some changes in Hp Fur protein resulted in increase of resistance to Mtz, and other changes resulted in decrease of resistance. Increase of Mtz resistance came from the enzyme's decreased ability of reducing prodrug Mtz to the form of bactericidal agent. Some sites that affects Mtz resistance (i) in Fur's N terminal extension, and (ii) in its central region, which links DNA binding and Fe-binding modules were identified. It was also found that the addition of FLAG tag to Fur's C terminus also significantly impairs Fur function.

• Proceedings of the PSK Conference
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• 2003.10b
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• pp.187.2-187.2
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• 2003

The cyclin-dependent kinases (CDKs), a group of serine/threonine kinases that form active heterodimeric complexes binding to cyclins, are key regulators of the cell cycle. The role of cyclin dependent kinases(CDKs) in cell cycle regulation has stimulated an interest in them as potential targets for proliferative diseases such as cancer, psoriasis, and chemotherapeutic agent-induced alopecia. Indirubin, an active ingredient of a traditional Chinese recipe Danggui Longhui Wan, are potent CDK inhibitors competing with ATP for binding to the catalytic site of the CDKs. (omitted)

• Biomedical Science Letters
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• v.30 no.3
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• pp.162-168
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• 2024

The discovery of a novel substance capable of regulating or suppressing platelet aggregation holds significant promise for the prevention and treatment of cardiovascular diseases. Artemisinin, a compound derived from plants like Artemisia or Scopolia, has demonstrated potential across various fields, including anticancer and Alzheimer's disease research. However, its specific role and mechanisms in influencing platelet activation and thrombus formation remain incompletely understood. This study delves into elucidating how artemisinin affects platelet activation and thrombus formation. Results revealed a significant increase in cAMP production with varying doses of artemisinin, alongside notable phosphorylation of VASP and IP₃R-both substrates for cAMP-dependent kinase. This phosphorylation led to the inhibition of Ca²⁺ mobilization from the dense tubular system, consequently reducing platelet activity via αIIb/β₃ inactivation and suppressing fibrinogen binding. Furthermore, artemisinin exhibited inhibition of thrombin-induced thrombus formation. These findings suggest that artemisinin holds promise as an effective prophylactic and therapeutic agent against cardiovascular diseases, specifically targeting abnormal platelet activation and thrombus formation.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.2
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• pp.247-255
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• 2014

Consumers have become very conscious about their nutrition and well being due to changes in their socio-economic lifestyle and rapid urbanization. Therefore, development of technology for production of low cost and functional meat products is urgently required. One such approach is innovative restructuring technology in which binding of meat pieces still remains the main challenge and extension of product is generally associated with poor binding and texture. Thus, the present study was envisaged as an attempt to solve this problem by the incorporation of flaxseed flour (FF) as bind enhancing agent. The FF was used at three different levels viz., 0.5%, 1%, and 1.5% to replace lean meat in pre-standardized restructured mutton chops formulation. The products were subjected to analysis for physico-chemical, sensory and textural properties. Cooking yield, moisture percentage and fat percentage increased with increase in the level of incorporation of FF, however, protein percent and pH decreased with increase in the level of incorporation. Shear force value of product incorporated with 1.5% FF was significantly higher (p<0.01) than control and product containing 0.5% FF level. Among the sensory attributes, product with 1% flaxseed flour showed significantly higher values (p<0.05) for general appearance, binding, texture and overall acceptability. Hardness showed significant increasing (p<0.01) values with increasing levels of incorporation of flaxseed flour, however all other parameters of texture profile analysis showed a decreasing trend. On the basis of sensory scores and physico-chemical properties, the optimum incorporation level of FF was adjudged as 1%. Products incorporated with optimum level of flaxseed flour (1%) were also assessed for water activity and microbiological quality during the storage period of 15 days. It was found that the extended restructured product could be safely stored under refrigeration ($4^{\circ}C{\pm}1^{\circ}C$) in low density polyethylene (LDPE) pouches for 15 days without marked deterioration in sensory and microbiological quality. Thus, it was concluded that flaxseed flour can be used as a good bind enhancing agent in extended restructured meat products at an economic cost.

• The Journal of the Korea Contents Association
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• v.10 no.6
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• pp.80-90
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• 2010

In this paper, we introduce some issues to implement an agent system to support service mobility in home network environment, and describe detailed design method in terminal as well as server agent. Specifically, we describe user recognition module, signaling message receiving/parsing module of terminal agent and signaling message receiving/parsing module, multimedia switching module, memory management module of server agent. We define several parameters managed in IP sharing device and design binding table structure to support mobility. And we utilize M/M/1/K queueing theory to obtain relations between memory size, blocking probability and memory utilization. From the obtained results, we show that memory size can be predicted in server agent mounted on IP sharing device.

• Archives of Pharmacal Research
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• v.12 no.2
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• pp.119-124
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• 1989

Cotton xanthate, which was obtained by treating cotton with carbon disulfide in alkaline solution, was treated with the solution of polyvalent metal ions to produce cotton xanthate-metal chelates. This chelation reaction was readily and simply achieved, and antimicrobial agents with suitable structures could subsequently be coupled to the chelate with ease at moderate pH values and in aqueous solution. Metal ions used in present work include Cu(II), Zn(II) and Fe(III). Tetracycline, streptomycin, neomycin and pyrithion were used as antimicrobial/antifungal agents. Antibacterial activities were measured employing ditch plate method against G(+) Staphylococcus aureus, Streptococus faecalis, and G(-) Escherichia coli, Enterobacter aerogenes, and the fungus, Aspergillus niger. All the cotton xanthate-metal-antimicrobial agent chelates exhibited activities whereas the cotton xanthate-metal chelates themselves were inactive. Considering the extensive washing procedures and results from control experiments, possibility of the involvement of physical adsorption for the binding of drugs could be excluded.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.04a
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• pp.325-325
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• 1994

본 연구에서는 먼저 14종의 flavonoid화합물을 대상으로 발암물질로서 잘 알려져있는 benzo(a)pyrene[B(a)P]에 대한 소핵생성억제효과를 관찰하였다. 소핵시험을 이용한 유전독성억제실험에서 비적적 큰 활성을 보이는 flavonoid는 2,3 이중결합과 3,5,7-trihydroxyl기를 갖는 polyhydroxy flavonol화합물들이었다. 이중에서 galangin은 활성이 비교적 컸으며, 이같은 유전독성억제효과는 galangin투여시 B(a)P의 대사활성화가 감소되고 활성본태산물들의 DNA binding을 저해함으로서 나타났다. 한편, galangin은 대사활성화가 필요없는 1차 발암물질인 N-methyl-N'-nitro-N-nitrosoguanidine(MNNG)에 의한 소핵생성도 감소시켰다. 이러한 galangin의 alkylating agent에 대한 유전독성억제효과는 calf thymus DNA를 이용한 실험에서 DNA의 메칠화를 저해하는 기전으로 나타나는 것으로 판단되었다. galangin은 mitouycin과 같은 DNA cross-linking agent에 의한 소핵생성에도 억제효과를 나타내었다. 특히 동시투여(simultaneous treatment)나 사후투여(post-treatment)시보다 사전투여(pre-treatment)시에 소핵생성억제효과가 컸으며 사전연속투여(multiple Pre-treatment)시에는 낮은 용량에서도 효과가 컸다. 이러한 저용량의 사전연용투여에 의한 유전독성억제효과들은 B(a)P나 MNNG에 대해서도 잘 나타났다.