• Journal of the Korean Society of Tobacco Science
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• v.20 no.1
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• pp.131-138
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• 1998

In order to minimize the trial frequency in the new filter cigarette design, we studied the relationship between smoke yield and tipping materials of cigarette. A three levels full factorial design involving filament denier (X1,2.5-3.3d), Porosity of the acetate filter plug wrap (X2, 3,500-16,000CU) and porosity of the tip paper (X3, 400-1,200CU) was used. Three independent factors (Xl, X2, X3) were chosen for their effects on the various responses and the function was expressed in terms of a quadratic polynomial equation, Y : $\beta$o + $\beta$1Xl + $\beta$2X2 + $\beta$3X3 + $\beta$11Xl2 + $\beta$22X22+ $\beta$33X32 + $\beta$12X1X2 + $\beta$13XIX3 $\beta$23X2X3 which measures the linear, quadratic, and interaction effects. Twenty-nine trial numbers were obtained as a results of using a three levels full factorial design and it was analyzed by the multiple regression analysis with backward stepwise in STATISTICA/pc under restricted conditions. Tar yields of the cigarette was affected by porosity of tip paper (0.66), filament denier (0.47) and porosity of plug wrap (0.28) in the decreasing order, and linear effect of tip paper porosity (B3) and filament denier (91) were significant at a level of 0.01($\alpha$). The filament denier and tipping paper porosity interaction F ratio among three factors had a P-value of 0,000041, indicating higher interaction between these factors. Based on the analysis of variance, the model fitted for Tar (Y1) was significant at 5% confidence level and the coefficient of determination (0.96) was the proportion of variability in the data fitted for by the model.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.13
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• pp.5181-5186
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• 2014

Tumors have evolved numerous mechanisms by which they can escape from immune surveillance. One of these is to produce immunosuppressive cytokines. Transforming growth factor-${\beta}$(TGF-${\beta}$) is a pleiotropic cytokine with a crucial function in mediating immune suppression, especially in the tumor microenvironment. TGF-${\beta}$ produced by T cells has been demonstrated as an important factor for suppressing antitumor immune responses, but the role of tumor-derived TGF-${\beta}$ in this process is poorly understood. In this study, we demonstrated that knockdown of tumor-derived TGF-${\beta}$ using shRNA resulted in dramatically reduced tumor size, slowing tumor formation, prolonging survival rate of tumor-bearing mice and inhibiting metastasis. We revealed possible underlying mechanisms as reducing the number of myeloid-derived suppressor cells (MDSC) and $CD4^+Foxp3^+$ Treg cells, and consequently enhanced IFN-${\gamma}$ production by CTLs. Knockdown of tumor-derived TGF-${\beta}$ also significantly reduced the conversion of na$\ddot{i}$ve $CD4^+$ T cells into Treg cells in vitro. Finally, we found that knockdown of TGF-${\beta}$ suppressed cell migration, but did not change the proliferation and apoptosis of tumor cells in vitro. In summary, our study provided evidence that tumor-derived TGF-${\beta}$ is a critical factor for tumor progression and evasion of immune surveillance, and blocking tumor-derived TGF-${\beta}$ may serve as a potential therapeutic approach for cancer.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.27 no.5
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• pp.446-456
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• 2005

Nerve growth factor(NGF) has a critical role in peripheral nerve regeneration. The aim of this study is to construct a well-functioning hNGF-$\beta$ recombinat adenovirus for the ultimate development of improved method to promote peripheral nerve regeneration with adenovirus mediated hNGF-$\beta$ gene transfection into Schwann cells. First PCR associated cloning of GFP-tagged hNGF-$\beta$ which was ligated into E1/E3 deleted adenoviral vector was performed and tranfected into E. coli to construct hNGF-$\beta$ recombinant adenovirus. After production of recombinat adenovirus in a large scale, its transfection efficiency, expression, and function were evaluated using cell lines or primarily cultured cells of HEK293 cells, Schwann cells, fibroblast(NIH3T3) and myocyte(CRH cells). GFP expression was observed in 90% of infected cells compared to uninfected cells. Total mRNA isolated from hNGF-$\beta$ recombinat adenoviru infected cells showed strong RT-PCR band, however, LacZ recombinant adenovirus infected or uninfected cells did not. NGF quantification by ELISA showed a maximal release of 18.865 +/- 0.31ng/mL at 4th day. PC-12 cells exposed to media with hNGF-$\beta$ recombinant adenovirus infected Schwann cell demonstrated higher levels of differentiation compared with controls. We generated hNGF-$\beta$ recombinant adenovirus and induced over expression of NGF successfully in nonneuronal and neuronal cells. Following these result, it is expected to develop an improved treatment strategy peripheral nerve regeneration using the hNGF-$\beta$ gene transfected cells.

• Korean Journal of Food Science and Technology
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• v.27 no.3
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• pp.375-379
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• 1995

Contents of ${\beta}-carotene$ and vitamin C in the vegetable(Angelica keiskei) juice were measured as a function of storing temperature$(-18^{\circ}C{\sim}35^{\circ}C)$ and period$(6{\sim}72\;hours)$. Content of ${\beta}-carotene$ was highest in the fresh juice and the degree of destruction of the ${\beta}-carotene$ was significantly increased as the storage temperature increased. Vitamin C in the juice stored at $4^{\circ}C$ decreased less than that of ${\beta}-carotene$. There was no significant difference in fatty acid compositions among in the fresh, freeze-dried juice and juice stored for 72 hours. Antioxidative activities of components extracted from the juice(fresh and stored for 24 hours) followed by incubating for 1 day were higher than that of ${\alpha}-tocopherol$ and then significantly decreased as incubation prolonged.

• The Pure and Applied Mathematics
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• v.11 no.1
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• pp.97-102
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• 2004

In this paper we establish some recurrence relations satisfied by quotient moments of upper record values from the Pareto distribution. Let ｛$X_n,n\qeq1$｝be a sequence of independent and identically distributed random variables with a common continuous distribution function(cdf) F($chi$) and probability density function(pdf) f($chi$). Let $Y_n\;=\;mas{X_1,X_2,...,X_n}$ for $ngeq1$. We say $X_{j}$ is an upper record value of {$X_{n},n\geq1$}, if $Y_{j}$＞$Y_{j-1}$,j＞1. The indices at which the upper record values occur are given by the record times ${u( n)}n,\geq1$, where u(n) = min{j｜j ＞u(n-l), $X_{j}$＞$X_{u(n-1)}$,n\qeq2$ and u(l) = 1. Suppose $X{\epsilon}PAR(\frac{1}{\beta},\frac{1}{\beta}$ then E$(\frac{{X^\tau}}_{u(m)}}{{X^{s+1}}_{u(n)})\;=\;\frac{1}{s}E$ E$(\frac{{X^\tau}}_{u(m)}{{X^s}_{u(n-1)}})$ - $\frac{(1+\betas)}{s}E(\frac{{X^\tau}_{u(m)}}{{X^s}_{u(n)}}$ and E$(\frac{{X^{\tau+1}}_{u(m)}}{{X^s}_{u(n)}})$ = $\frac{1}{(r+1)\beta}$ [E$(\frac{{X^{\tau+1}}}_u(m)}{{X^s}_{u(n-1)}})$ - E$(\frac{{X^{\tau+1}}_u(m)}}{{X^s}_{u(n-1)}})$ - (r+1)E$(\frac{{X^\tau}_{u(m)}}{{X^s}_{u(n)}})$]

• Journal of the Korean Vacuum Society
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• v.5 no.4
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• pp.301-308
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• 1996

The heat of desorption and the work function change induced by nitrogen adsorption on the slepped tungstein surface plants, W(210) and W(310), are measured using the Field Electron Emission Microscope(FEM). The adsoption sites are predicted from the Thermal Desortion Spectra(TDS). The wirk function change of both W(210) and W(310) planes increase as increasing the nitrogen dose and saturates at the nitrogen dose about 5 Langmuir to 0.29 eV and 0.20 eV respectively. We find three adsorption site on each plane for the low dose range. The TDS result shows that the intensity of $\alpha_1$ state on W(310) is much stronger than that of $\alpha_1$ state on W(210), and the direction of nitrogen dipole moment adsorbed on the sites correspond to $\alpha_1$ and $\beta_2$ state on W(210) and W(310) planes are in the opposite direction to that of the equivalent states on W(100) plane. From this observation we can predict the relative atomic position in the z-direction (perpendicular direction to the surface) of nitrogen molecules/atoms adsorbed on these sites.

• Journal of Korean Society of Steel Construction
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• v.15 no.4 s.65
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• pp.389-401
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• 2003

The purpose of this study is to develop an algorithm, which can be designed the optimal sections of the composite framed structures constituted with the beams and the columns consisted of H type of steel section and concrete considering the reliability index. The optimized problem or the composite framed structures is formulated with the objective function and the constraints taking the section sizes as the design variables. The objective functions are constituted by the total costs of constructions. Also, the constraints are derived by considering the reliability index of section stress and allowable stress. The algorithm optimized the section of the composite framed structures utilizes the SUMT method using the modified Newton-Raphson direction method. The optimizing algorithm developed in this study is applied to the numerical examples with respecting a one-bay, one-story composite framed structure and a one-bay five-story one for the practical utilization of design on the composite framed structures using the reliability indices$({\beta})$ three and zero. In addition, their numerical results are compared and analyzed to examine the possibility of optimization the applicability, and the convergence this algorithm.

• Asian-Australasian Journal of Animal Sciences
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• v.29 no.8
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• pp.1065-1074
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• 2016

Growth factors play an important role during early ovarian development and folliculogenesis, since they regulate the migration of germ cells to the gonadal ridge. They also act on follicle recruitment, proliferation/atresia of granulosa cells and theca, steroidogenesis, oocyte maturation, ovulation and luteinization. Among the growth factors, the growth differentiation factor 9 (GDF9) and the bone morphogenetic protein 15 (BMP15), belong to the transforming growth factor beta (TGF-${\beta}$) superfamily, have been implicated as essential for follicular development. The GDF9 and BMP15 participate in the evolution of the primordial follicle to primary follicle and play an important role in the later stages of follicular development and maturation, increasing the steroidogenic acute regulatory protein expression, plasminogen activator and luteinizing hormone receptor (LHR). These factors are also involved in the interconnections between the oocyte and surrounding cumulus cells, where they regulate absorption of amino acids, glycolysis and biosynthesis of cholesterol cumulus cells. Even though the mode of action has not been fully established, in vitro observations indicate that the factors GDF9 and BMP15 stimulate the growth of ovarian follicles and proliferation of cumulus cells through the induction of mitosis in cells and granulosa and theca expression of genes linked to follicular maturation. Thus, seeking greater understanding of the action of these growth factors on the development of oocytes, the role of GDF9 and BMP15 in ovarian function is summarized in this brief review.

• The Korean Journal of Physiology and Pharmacology
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• v.18 no.2
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• pp.155-161
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• 2014

Overexpression of amyloid precursor protein with the Swedish mutation causes abnormal hyperphosphorylation of the microtubule-associated protein tau. Hyperphosphorylated isoforms of tau are major components of neurofibrillary tangles, which are histopathological hallmarks of Alzheimer's disease. Protein phosphatase 2A (PP2A), a major tau protein phosphatase, consists of a structural A subunit, catalytic C subunit, and a variety of regulatory B subunits. The B subunits have been reported to modulate function of the PP2A holoenzyme by regulating substrate binding, enzyme activity, and subcellular localization. In the current study, we characterized regulatory B subunit-specific regulation of tau protein phosphorylation. We showed that the PP2A B subunit PPP2R2A mediated dephosphorylation of tau protein at Ser-199, Ser-202/Thr-205, Thr-231, Ser-262, and Ser-422. Down-regulation of PPP2R5D expression decreased tau phosphorylation at Ser-202/Thr-205, Thr-231, and Ser-422, which indicates activation of the tau kinase glycogen synthase kinase 3 beta ($GSK3{\beta}$) by PP2A with PPP2R5D subunit. The level of activating phosphorylation of the $GSK3{\beta}$ kinase Akt at Thr-308 and Ser-473 were both increased by PPP2R5D knockdown. We also characterized B subunit-specific phosphorylation sites in tau using mass spectrometric analysis. Liquid chromatography-mass spectrometry revealed that the phosphorylation status of the tau protein may be affected by PP2A, depending on the specific B subunits. These studies further our understanding of the function of various B subunits in mediating site-specific regulation of tau protein phosphorylation.

• Journal of Oriental Neuropsychiatry
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• v.18 no.2
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• pp.89-100
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• 2007

Objective : The purpose of this study is to examine relationship between cognitive function and sasang constitution by analyzing EEG status of company workers in Cheon-An. Method : 59 company workers were tested with cognitive assessment EEG program and questionaire for the Sasang Constitution Classification II. They were assorted by Sasang Constitutions, and we analyzed its correlation with cognitive assessment score and EEG data. Results : 1. According to mean active EEG rhythm of Alpha. H-Beta, Gamma wave, there were no significant difference among Sasang Constitution. 2. According to mean success, error, concentration, response, workload and left/right brain activity score, there were no significant difference among Sasang Constitution. 3. According to mean active EEG rhythm of Theta, SMR, M-Beta wave, Soyangin(少陽人)'s value was significantly higher than that of Taeumin(太陰人) 4. According to mean cognitive strenghth score, Soyangin(少陽人)'s value was significantly higher than that of Taeumin(太陰人). Conclusion : In conclusion, Sasang Constitutional difference has no relevance with cognitive abilities However, Soyangin(少陽人) showed higher mean active EEG rhythm of Theta, SMR, M-Beta wave than that of Taeumin(太陰人). In addition, Soyangin(少陽人) also showed higher mean cognitive strenghth score than that of Taeumin(太陰人).