• Title/Summary/Keyword: beta-HAD

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The Effects of Servicescapes in Korean Restaurants on Customers' Experiential Value, Pleasure feeling and Customer Satisfaction (국내 한식당의 서비스스케이프가 고객의 경험적 가치, 감정적 반응 및 고객 만족도에 미치는 영향에 관한 연구)

  • Jung, Hyo-Sun;Yoon, Hye-Hyun
    • Journal of the Korean Society of Food Culture
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    • v.25 no.1
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    • pp.36-46
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    • 2010
  • The purpose of this study was to investigate the effects of servicescapes in Korean restaurants on customers' experiential value, pleasure feeling and customer satisfaction. Based on a total of 550 samples obtained from empirical research, this study reviewed the reliability and fitness of research model using the Amos program. The relationships hypothesized in the model were tested simultaneously by using a structural equation model (SEM). The proposed model provided an adequate fit to the data, $\chi^2=406.097$ (df 130), p<.001, GFI .915, AGFI .889, RMR .042, NFI .955, CFI .969, RMSEA .062. SEM results showed that the servicescape showed a positive significant effect on customers' experiential value ($\beta=.808$, t=15.171, p<.001), and customers' experiential value had a positive significant effect on pleasure feeling ($\beta=.756$, t=10.616, p<.001). Also, customer's experiential value ($\beta=.391$, t=8.579, p<.001) and pleasure feeling ($\beta=.573$, t=13.091, p<.001) had a positive significant effects on customer satisfaction. Analysis of mediating roles showed that, the effect of servicescapes in Korean restaurants on customers' pleasure feeling was perfectly mediated by the customers' experiential value. Limitations of this study and future research directions are also discussed.

The Effects of Hotel Employees' Physical Attractiveness on Person-job Fit - Focused on the Mediating Roles of Self-esteem and Self-efficacy - (호텔 직원의 신체적 매력도가 개인직무적합성에 미치는 영향 - 자아존중감과 자기효능감의 매개효과를 중심으로 -)

  • Jung, Hyo-Sun;Choi, Soo-Keun;Yoon, Hye-Hyun
    • Journal of the Korean Society of Food Culture
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    • v.24 no.6
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    • pp.711-720
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    • 2009
  • The purpose of this study was to understand the effects of hotel employees' physical attractiveness on person-job fit and to empirically analyze whether self-esteem and self-efficacy play a mediating role in the causality between an employee's physical attractiveness and person-job fit. Self-administered questionnaires were completed by 345 employees and the data were analyzed by frequency analysis, factor analysis, reliability analysis, correlation analysis and multiple regression analysis. The primary results were as follows: Multiple regression analysis showed that hotel employee physical attractiveness had a positive significant influence on self-esteem ($\beta=.504$, p<.001) and self-efficacy ($\beta=.441$, p<.001). Also, employee selfesteem ($\beta=.281$, p<.001) and self-efficacy ($\beta=.478$, p<.001) each had a positive significant influence on person-job fit. As a result of analyzing the mediating role, the effect of hotel employees' physical attractiveness on person-job fit was partially mediated by self-esteem and self-efficacy.

Detection of beta-lactam antibiotic resistant genes in Escherichia coli from porcine fecal samples using DNA chip

  • Park, Nam-Yong;Na, Sung-Ho;Cho, Ho-Seong
    • Korean Journal of Veterinary Service
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    • v.30 no.4
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    • pp.505-510
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    • 2007
  • This study was conducted to detect ${\beta}$-lactam antibiotic-resistant genes in the 400 E coli isolates from porcine fecal samples in Korea by a DNA chip. The DNA chip contains the specific probe DNAs of the ${\beta}$-lactam antibiotic-resistant genes that had been labeled with a mixture of primer set designed to amplify specific genes (PSE, OXA, FOX, MEN, CMY, TEM, SHV, OXY and AmpC) using a multiplex polymerase chain reaction (PCR). Of 400 isolates 339 contained at least one ${\beta}$-lactamases gene. Resistance to ${\beta}$-lactamases was mediated mainly by AmpC (n = 339, 100%), and followed by TEM (n = 200, 59.0%), CMY (n = 101, 29.8%), PSE (n = 30, 8.9%) and both OXA and SHV genes (n = 20, 5.9%), while the FOX, MEN and OXY genes were not detected. The other sixty-one did not contain any ${\beta}$-lactamase genes even though they were resistant to antimicrobial drugs. In conclusion, the DNA chip system can be used as a rapid and reliable method for detecting of ${\beta}$-lactamases genes, which will help veterinarians select the antibiotics for monitoring and treating of animal diseases.

Study of the Percutaneous Absorption, Stability and Physicochemical Properties of $OMP-{\beta}-CD$ Inclusion Complex ($OMP-{\beta}-CD$ 포접화합물의 물리화학적 성질, 안정성 및 피부 투과 실험)

  • Lee, Sang-Young;Lee, Gye-Won;Choi, Hyun-Soon;Jee, Ung-Kil
    • Journal of Pharmaceutical Investigation
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    • v.27 no.4
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    • pp.271-277
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    • 1997
  • Because omeprazole(OMP) is very unstable in aqueous condition, $OMP-{\beta}-CD$, the inclusion complex of OMP and ${\beta}-cyclodextrin({\beta}-CD)$ was made and physicochemical properties of it were compared with those of OMP. Skin permeability of OMP and $OMP-{\beta}-CD$ in propylene glycol vehicle and the reciprocal action of ${\beta}-CD$ with various enhancers were examined through hairless mouse. Adhesive patches were prepared with polyisobutylene and the skin permeability and stability of OMP were investigated. The inclusion complex showed higher solubility and lower partition coefficient than OMP itself. DMSO, 1-methyl 2-pyrrolidone and sodium cholate had an enhancing effect. However ethanol and polysorbate 80 hardly showed the enhancing effect of OMP. When sodium lauryl sulfate and sodium cholate as enhancer were added in patch, the former case showed higher permeability of OMP.

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Correlates of Depression and Perceived Health Status in Patients with Atrial Fibrillation (심방세동 대상자의 우울, 주관적 건강지각 관련요인)

  • Kang, Youn-Hee
    • Korean Journal of Adult Nursing
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    • v.21 no.1
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    • pp.86-94
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    • 2009
  • Purpose: The purpose of this study was to determine correlates of depression and perceived health status in patients with atrial fibrillation. Methods: The study utilized a descriptive correlational survey design. A convenience sample of 115 subjects were recruited from 3 tertiary medical centers. The data were analyzed by descriptive statistics, hierarchical multiple regression. Results: 1) Study subjects perceived the low frequency of symptoms, relatively high social support, low depression, moderately low physical health, and mental health. 2) The 38% of variance in depression was significantly explained by symptom(${\beta}$ = .49), social support(${\beta}$ = -.21), and education(${\beta}$ = -.17). 3) The 40% of variance in physical health was significantly explained by symptom(${\beta}$ = -.55), social support(${\beta}$ = .16), and education(${\beta}$ = .20). 4) the 12% of variance in mental health was significantly explained by symptom(${\beta}$ = -.26), and social support(${\beta}$ = .24). Conclusion: Most health care providers assume atrial fibrillation patients have very few symptoms. However, symptom related to atrial fibrillation was found to be the most important factors in explaining depression and perceived health status. Additionally, higher social support had great influences on the lower depression and higher perceived health status. Based on the findings of this study, directions for nursing practice and further nursing research for patients with atrial fibrillation are suggested.

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Purification and Characterization of the Bacillus sp. KK-l $\beta$-Xylosidase from a Recombinant Escherichia coli

  • Jung, Kyung-Hwa;Chun, Yong-Chin;Lee, Jae-Chan;Park, Seung-Hwan;Yoon, Ki-Hong
    • Journal of Microbiology and Biotechnology
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    • v.8 no.3
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    • pp.258-263
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    • 1998
  • ${\beta}$-Xylosidase was purified from the recombinant Escherichia coli carrying the Bacillus sp. KK-1 ${\beta}$-xylosidase gene (xylB). The molecular mass of the purified enzyme was estimated to be 62 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. However, the apparent molecular mass of the ${\beta}$-xylosidase was 140 kDa, indicating that the native ${\beta}$-xylosidase has an oligomeric structure composed of two identical subunits. The isoelectric point was determined to be pH 5.5. The enzyme was highly active on p-nitrophenyl-$\beta$-D-xylopyranoside but it barely hydrolyzed xylan substrates, and did not exhibit activity towards carboxymethylcellulose and p-nitrophenyl-${\beta}$-D- glucopyranoside. The enzyme had a pH optimum for its activity at pH 6.5 and a temperature optimum at $40^{\circ}C$. The enzyme activity was completely inhibited by the presence of $Hg^{++}$, and also markedly inhibited by D-xylose and D-glucose.

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Effects of Creative Nursing Practice on Hospital Nurses' Job Satisfaction and Organizational Commitment (간호사의 창의적 간호실무가 직무만족 및 조직몰입에 미치는 영향)

  • Kang, So-Young
    • Journal of Korean Academy of Nursing Administration
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    • v.18 no.2
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    • pp.234-243
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    • 2012
  • Purpose: This study was done to: (a) examine relationships between creative nursing practice and job satisfaction, and organizational commitment that nurses perceive in a hospital, and (b) identify creative nursing practice factors affecting job satisfaction and organizational commitment. Method: Using self-report questionnaires including 25 items of the Creative Nursing Practice Index, a descriptive study was conducted with a sample of 387 nurses working in the nursing care units of a general hospital in Seoul, Korea. Data were analyzed, using independent t-test, ANOVA, Pearson correlation, and hierarchical multiple regression analysis. Result: The mean score for nurses performance of creative practice was $2.29{\pm}0.46$ (range 1 to 4). Creative nursing practice had positive correlation with nurses' job satisfaction (r=.427, p<.001) and organizational commitment (r=.433, p<.001). Creative nursing practice factors influencing job satisfaction were implementing customized care (${\beta}$=.158, p=.010), making new knowledge (${\beta}$=.257, p<.001), and searching ideas (${\beta}$=.209, p<.001). Nurses' organizational commitment was influenced by implementing customized care (${\beta}$=.192, p=.002), making new knowledge (${\beta}$=.158, p=.028), searching ideas (${\beta}$=.254, p<.001), sharing ideas (${\beta}$=.250, p=.003), and validating ideas (${\beta}$=.189, p=.036). Conclusions: The results indicate a need to develop nurses' creative competency to pursue quality care as well as increase nurses' job satisfaction and organizational commitment.

A Gene Encoding $\beta$-amylase from Saprolegnia parasitica and Its Expression in Saccharomyces cerevisiae

  • Kim, Hee-Ok;Park, Jeong-Nam;Shin, Dong-Jun;Lee, HwangHee Blaise;Chun, Soon-Bai;Bai, Suk
    • Journal of Microbiology and Biotechnology
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    • v.11 no.3
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    • pp.529-533
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    • 2001
  • The ${\beta}$-Amylase cDNA fragment from the oomcete Saprolegnia parasitica was cloned by reverse transcription-polymerase chain reaction (RT-PCR) using degenerate oligonucleotide primers derived from conserved ${\beta}$-amylase sequences. The 5'and 3'regions of the $\beta$-amylase gene were amplified using the rapid amplification of cDNA ends (rACE) system. It consisted of an open reading frame of 1,350 bp for a protein of 450 amino acids. Comparison between the genomic and cDNA sequences revealed that the intron was not present in the coding region. The deduced amino acid sequence of the ${\beta}$-amylase gene had a 97% similarity to the ${\beta}$-amylase of Saprolegnia ferax, followed by 41% similarity to those of Arabidopsis thaliana, Hordeum vulgare, and Zea mays. The ${\beta}$-amylase gene was also expressed in Saccharomyces cerevisiae by placing it under the control of the alcohol dehydrogenase gene (ADC1) promoter.

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Improvement of Insoluble $\beta$-Glucosidase Activity by Molecular Chaperonin GroEL/ES (Inclusion Body를 형성한 $\beta$-Glucosidase의 Chaperonin에 의한 활성 향상)

  • Kim, Jong-Deok;Sachiko Machida;Kiyoshi Hayashi;Ha, Sun-Deok;Gong, Jae-Yeol
    • KSBB Journal
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    • v.14 no.4
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    • pp.429-433
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    • 1999
  • $\beta$-Glucosidaes from Cellvibrio gilvus(CG) was successfully overproduced in soluble form in E. coli with the coexpression of GroEL/ES/. Without the GroEL/ES protein, the $\beta$-glucosidase overexpressed in E. coli constituted a huge amount(80%) of total cellular protein, but was localized in the insoluble fraction, and little activity was detected in the soluble fraction. Coexpression of the E. coli GroEL/ES had a drastic impact on the proper folding of the $\beta$-glucosidase; 20% of the overexpressed enzyme was recovered in the soluble fraction in active form. Similar effects of GroEL/ES were also observed on the overexpressed $\beta$-glucosidase from Agrobacterium tumefaciens(AT). And pET28(a)-RGRAR, partially deleted mutant lacking 5-amino acid residues at carboxy teminus also could be folded into an active form when expressed with the molecular chaperonin GroEL/ES, and its activity was higher than that of the without GroEL/ES system, In addition, the synergistic effect of GroEL/ES and the low induction temperature were important factors for solubilization of the inclusion body from overproduced $\beta$-glucosidases.

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Relations Between Communication Satisfaction and Group Creativity in Organization (조직 구성원의 커뮤니케이션 만족이 인적자원의 집단 창의성에 미치는 영향에 관한 연구)

  • Jang, Chung-Seok;Park, Jong-Oh
    • Management & Information Systems Review
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    • v.21
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    • pp.49-76
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    • 2007
  • The main purpose of this study is to assess the relationship between communication satisfaction and group creativity. To achieve this purpose, this study has two objectives. First, I will identify the relationship between the satisfaction of communication and idea creation. Second, the relationship between communication satisfaction and problem solving activity will be statistically tested. The major findings of the study can be summarized as follows: First, Statistically significant positive relations were found between the communication satisfaction and idea creation. Especially, sub-construct for supervisor communication$({\beta}=0.14,\;p<.05)$, quality of media$({\beta}=0.13,\;p<.05)$, individual feedback$({\beta}=0.36,\;p<.001)$, organizational prospect$({\beta}0.31,\;p<.001)$ showed the statistically significant positive(+) relationship with construct in idea creation. Second, the measurement of communication satisfaction had showed statistically significant relationship with the problem solving activity. Especially, communication with supervisor$({\beta}=0.21,\;p<.05)$, quality of media$({\beta}=0.21,\;p<.001)$, organizational prospects$({\beta}=0.22,\;p<.001)$, and communication with subordinate$({\beta}=0.37,\;p<.001)$ showed the significant positive(+) relationship with problem solving activity construct. These consequences suggest that communication satisfaction of employee is effective in the improving the group creativity in organization. In the conclusion, this study present the satisfaction factor of employee communication as a incremental factor of group creativity.

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