• Title/Summary/Keyword: berberine sulphate

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Detection of Mycoplasma-like Organisms in Some Trees by Fluorescence Microscopy with Berberine Sulphate (Berberine Sulphate를 이용(利用)한 형광현미경기법(螢光顯微鏡技法)에 의(依)한 수목(樹木)마이코플라스마검정(檢定))

  • Bak, Won Chull;La, Yong Joon
    • Journal of Korean Society of Forest Science
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    • v.80 no.2
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    • pp.232-236
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    • 1991

  • The efficacy of berberine sulphate, a fluorochrome having binding properties with both DNA and RNA, was investigated for the detection of mycoplasma-like organisms(MLOs) in jujube(Zizyphus jujuba), paulownia(Paulownia tomentosa), mulberry(Morus alba) trees and periwinkle (Catharanthus roseus) plant. When examined under fluorescence microscope, berberine sulphate-stained sections of diseased samples showed distinct MLO-specific fluorescent particles in the phloem area, while such fluorescence was absent in the healthy ones. This staining technique was proved to be a very accurate method for the diagnosis of MLO infections in woody and herbaceous plants. Furthermore, the cheap and easy procedure could be used to test a great number of samples on MLO infections with reliability and rapidity.

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