• International Journal of Oral Biology
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• v.35 no.4
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• pp.169-175
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• 2010

Cholinesterase (ChE) is one of the most ubiquitous enzymes and in addition to its well characterized catalytic function, the morphogenetic involvement of ChE has also been demonstrated in neuronal tissues and in non-neuronal tissues such as bone and cartilage. We have previously reported that during mouse tooth development, acetylcholinesterase (AChE) activity is dynamically localized in the dental epithelium and its derivatives whereas butyrylcholinesterase (BuChE) activity is localized in the dental follicles. To test the functional conservation of ChE in tooth morphogenesis among different species, we performed cholinesterase histochemistry following the use of specific inhibitors of developing molar and incisors in the hamster from embryonic day 11 (E11) to postnatal day 1 (P1). In the developing molar in hamster, the localization of ChE activity was found to be very similar to that of the mouse. At the bud stage, no ChE activity was found in the tooth buds, but was first detectable in the dental epithelium and dental follicles at the cap and bell stages. AChE activity was found to be principally localized in the dental epithelium whereas BuChE activity was observed in the dental follicle. In contrast to the ChE activity in the molars, BuChE activity was specifically observed in the secretory ameloblasts of the incisors, whilst no AChE activity was found in the dental epithelium of incisors. The subtype and localization of ChE activity in the dental epithelium of the incisor thus differed from those of the molar in hamster. In addition, these patterns also differed from the ChE activity in the mouse incisor. These results strongly suggest that ChE may play roles in the differentiation of the dental epithelium and dental follicle in hamster, and that morphogenetic subtypes of ChE may be variable among species and tooth types.

• Journal of Periodontal and Implant Science
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• v.32 no.1
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• pp.1-12
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• 2002

The periodontal ligament(PDL) is a unique tissue that is crucial for tooth function. However, little is known of the molecular mechanisms controlling PDL function. PDL-specific protein;PDLs22 had been previously identified as a novel protein isolated from cultured human PDL fibroblasts using subtraction hybridization between human gingival fibroblasts and PDL fibroblasts. The aim of this study was to examine the expression pattern and tissue localization of PDLs22 protein in embryonic and various postnatal stages of developing mouse using immunohistochemical staining. Embryos (E18) and postnatal (P1, P4, P5, P15, P18) were decapitated and the heads were fixed overnight in a freshly prepared solution of 4% paraformaldehyde. Some specimens were decalcified for $2{\sim}4$ weeks in a solution containing 10% of the disodium salt of ethylenediamine-tetraacetic acid (EDTA). Next, tissues were dehydrated, embedded in paraffin and sectioned serially at $6{\mu}m$ in thickness. Polyclonal antiserum raised against PDLs22 peptides, ISNKYLVKRQSRD, were made. The localization of PDLs22 in tissues was detected by polyclonal antibody against PDLs22 by means of immunohistochemical staining. The results were as follows; 1. Expression of PDLs22 protein was not detected in the tooth germ of bud and cap stage. 2. At the late bell stage and root formation stage, strong expression of PDLs22 protein was observed in developing tooth follicle, osteoblast-like cells, and subodontoblastic cells in the tooth pulp, but not in gingival fibroblasts, ameloblasts and odontoblasts of tooth germ 3. In erupted tooth, PDLs22 protein was intensely expressed in PDL and osteoblast-like cells of alveolar bone, but not in gingival fibroblasts, mature osteocytes and adjacent salivary glands. 4. In the developing alveolar bone and mid-palatal suture, expression of PDLs22 protein was seen in undifferentiated mesenchymal cells and osteoblast-like cells of developing mid-palatal suture, but not in mature osteocytes and chondrocytes. These results suggest that PDLs22 protein may play an important role in the differentiation of undifferentiated mesenchymal cells in the bone marrow and PDL cells, which can differentiate into multiple cell types including osteoblasts, cementoblasts, and PDL fibroblasts. However, more researches should be performed to gain a better understanding of the exact function of PDLs22 protein which related to the PDL cell differentiation.

• Advances in pediatric surgery
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• v.9 no.1
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• pp.19-23
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• 2003

Necrotizing Enterocolitis (NEC) is usually a disease of premature infants, but occasionally it affects the term neonate. Twenty-five infants with NEC were treated at Asan Medical Center between January 2000 and December 2002, and 13 of them were term infants. In each case, the diagnosis of NEC was established by a clinical illness fulfilling the Bell's stage II or III NEC as modified by Walsh or by surgical findings. There were six males and seven females. The birth weight was from 1,960 to 3,700 g. The age at diagnosis was from 1 to 40 days. Four patients had congenital heart disease: one of who had hypothyroidism and cleft palate. Abdominal distension was present in all, and bloody stools in four. One patient had history of hypoglycemia, three had Rota viral infection. Eight patients had leucopoenia (<$5.0{\times}10^9/L$), seven had thrombocytopenia (<$100{\times}10^9/L$), and three severe thrombocytopenia (<$50{\times}10^9/L$). Laparotomy was required in 10 of the 13 patients. Indications for operation in the acute phase were failure to respond to aggressive medical therapy in five, and perforation in three patients. There were two late phase operations for intestinal stricture and fistula. There were no operative complications. Ten of thirteen patients survived (76.9%). Two patients died of septic complication. There was a delayed death due to heart failure. There was a significant difference in survival according to platelet count ($50{\times}10^9/L$) (p<0.05). Congenital heart disease and Rota viral infection are associated with NEC in term infants and thrombocytopenia and leucopoenia may be surgical indications.

• Composites Research
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• v.13 no.4
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• pp.42-53
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• 2000

A study has been made to establish an optimum condition in the surface treatment and curing method that is important for the fabrication of Al 7075/CFRP laminates. PAA(Phosphoric Acid Anodizing) provided a good adhesive strength and FPL(Sulfuric / Sodium Dichromate Acid Etching) had a similar adhesive strength with PAA. On the other hand, the poor adhesive strength was shown on vapor degrease and CAA(Chromic Acid Anodizing). By using the atomic force microscope(AFM), it was found that the PAA oxide surface obviously had a greater degree of microroughness as compared to vapor degrease, CAA and FPL treated surfaces. These results support the concept of a mechanical interlocking of the adhesive with-in the oxide pores as the predominant adhesion mechanism. In curing methods, the adhesive strength of co-curing method was higher than that of secondary curing method. With respect to stability of specimen shape, the secondary curing method was better than co-curing method. DMA(Dynamic Mechanical Analysis) test revealed $T_g$ in curing times over 60 min is nearly same, so it is estimated they will have similar degree of curing and joint durability in using FM300M adhesive film.

• Elastomers and Composites
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• v.39 no.1
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• pp.12-22
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• 2004

The plasma surface modification of natural rubber vulcanizate was carried out using chlorodifluoromethane in a radio-frequency (13.56 MHz) electrodeless bell type plasma reactor. The modification was qualitatively assessed by Fourier transform infrared spectroscopy. The frictional force of the plasma-treated surface was found to decrease with the time of plasma treatment. An increase in the surface polarity, as evidenced by the decrease in contact angle of a sessile drop of water and ethylene glycol on the natural rubber vulcanizate surface, was noted with the plasma modification. In the case of similar plasma treatment of glass surface, only a reduction in the polarity was observed. The use of geometric and harmonic mean methods was found to be useful to evaluate the London dispersive and specific components of surface free energy. Irrespective of the method used for evaluation, an increasing trend in the surface free energy was noted with increasing plasma treatment time. However, the harmonic mean method yielded comparatively higher values of surface free energy than the geometric mean method. The plasma surface modification was found to vary the frictional coefficient by influencing the interfacial, hysteresis and viscous components of friction in opposing dual manners.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• v.3 no.1
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• pp.147-157
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• 1992

The fragile X syndrome, which is considered to be synonymous with the Martin-Bell syndrome, is a relatively common form of X-linked mental retardation. The syndrome seems to occure in many different ethnic groups and its prevalence among mentally retarded males has been estimated to be in the order of 2 to 6%. The karyotypic hallmark of the syndrome is made up with a pronounced constriction near each tip of the long arm of the X chromosome(fragile site), shown in vitro only under conditions in which thymidylate production is blocked(lowered folate levels). Special culture media are needed to demonstrate this constriction site. Major clinical features associated with the syndrome include macroorchidism, large or prominent ears, significant emotional and behavioral dysfunctions such as hyperactivity, self-injury, lack of eye contact and social interaction, schizophrenia, autism, etc., and speech and language dysfunctions ranging from nonverbal to verbal speech with moderate to severe expressive language delays. Some have minor clinical features in common such as an increase in birth weight high forehead, prognathism, increased head circumference in infancy and childhood which did not persist into adult life. The recent research findings have shown that the fragile X syndrome is associated with infantile autism. Many patients with the fragile X syndrome fulfill the diagnostic criteria for infantile autism. Therefore it is recommendable that any patient with developmental delays and autism or autistic manifestations should have a chromosomal analysis, including fragile X examination. In the present review, historical aspects, incidence, and clinical features are presented. Recent anecdotal reports of the association with autism and the clinical improvement following high dose folic acid treatment will be discussed.

• Journal of Conservation Science
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• v.31 no.1
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• pp.65-73
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• 2015

The purposes of this study are helping the understanding on the Dae-hwe kind relics, verifying the deformation rate stability during conservation treatment and enhancing the conservation treatment work by investigating the status and analyzing the material of Taejo's portrait mounting decorative knots in the National Palace Museum of Korea collection. The result of material analysis on the decorative knots was that the outer threads are silk threads, inner threads are cotton threads, the width of the metal threads was $500{\sim}600{\mu}m$ and the major component of it was 55wt% copper. The result of verification on the deformation rate during the form correction of the bell tassel was that it has been found as stable by having the deformation rate of 1 TPCM or less. The result of glue selection test to be used on the repairing of tassel cross-section with its thread cut was that wheat starch adhesive has been found most appropriate because it has the highest tensile strength at all concentrations. The conservation and treatment procedure was established based on above results of analysis and test on the relic and the conservation treatment work including packaging has been completed in accordance with the established procedure.

• Analytical Science and Technology
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• v.13 no.4
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• pp.403-411
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• 2000

This study described a method of thermoluminescence dating of pottery shards using subtraction method. TL measurement was achieved using two different types of samples prepared by quartz inclusion method and fine-grain technique. Fine grains (size range: $5-10{\mu}m$) were separated by suspending grounded pottery samples into acetone solution and sedimentation quantitatively. In quartz inclusion method quartz grains in the size range of 90 to $125{\mu}m$ diameter were obtained by extracting the quartz crystals embed in the pottery shards and etching them with 1.0 M HF solutions. The archaeological dose of both the quartz and fine grains was determined from the dose calibration curves obtained from sequential irradiation of $^{137}Cs$ gamma and $^{241}Am$ alpha source to the samples and TL measurement of natural samples, in which the alpha dose of 4.60 Gy for the Packjae pottery was obtained using subtraction method. Annual alpha dose rates ($3.05{\pm}0.11$ mGy/yr.) were determined by the analysis of U, Th contents in the pottery shards and evaluation of the values with Bell's equation. Dividing the alpha dose accumulated in the pottery shards by the annual alpha dose rate, we found age of approximately $1508{\pm}80$ years B.P. (AD. ca. 492 yr.) for the Packjae pottery. It matches well with the archeological age estimate (middle of 5th century) within 10 percent uncertainty and thereby conforms the age of the pottery sample.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.11 no.7
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• pp.2708-2712
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• 2010

Amines, ammonia or 3-methoxypropylamine (MPA), are used to maintain the optimized pH for the prevention of corrosion in the secondary side of Pressurized Water Reactors (PWRs). They are differently dissociated as a function of temperature which is not same in each location of the water-steam cycle. pH at the operation temperature depends on temperature of fluid and equilibrium constants of water and amines. Thus, every amine provides the different pH in the entire secondary side so that pH is not only the sufficient parameter in corrosion control. The secondary parameter, i.e., buffer intensity, is the ability to maintain a stable pH when $H^+$ are added or removed due to the ingress of impurities or the reaction of corrosion. The buffer intensity is necessary to provide the selection criteria for the best pH control agent for secondary side and the basic understanding of the reason why the flow-accelerated corrosion(FAC) rate may demonstrate the bell-shape curve over temperature. The buffer intensities of ammonia and MPA were reviewed over the entire operation temperature of PWRs. The sufficient buffer intensity is provided for the inhibition of corrosion by ammonia in low temperature $(25{\sim}100^{\circ}C)$ and by DMA in high temperature $(150{\sim}250^{\circ}C)$. In terms of buffer intensity, i) the best pH control agent is an amine with $pK_a(T)$ range of pH(T)- $1{\leq}pK_a(T){\leq}pH(T)$ + 0.5 and ii) the amine solution should have sufficient buffer intensity, ${\beta}$ to inhibit corrosion, and iii) FAC rate may be maximum at the temperature, where ${\beta}_B/{\beta}$ ratio is lowest.

• Journal of the Korean Institute of Gas
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• v.7 no.2 s.19
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• pp.22-32
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• 2003

Al-brass material is generally used at the state of plastic deformation, for example; bending, extension of bell mouth at shell and tube type heat exchanger. And SCC(stress corrosion cracking) of Al-brass material will be affected by residual stress as plastic deformation. SCC results from synergism between mechanical factor and corrosion environment. Mechanical factor is stress that directly relates with stress intensity factor at the crack tip. This paper was studied on the effect of stress on SCC of Al-brass tube under in $3.5\%$ NaCl. + $0.1\%\;NH_4OH$ solution by constant displacement tester. Increasing of acidified water flow into sea and speeds up corrosion rate of Al-brass which is used as a tube material of vessel heat exchanger by polluted coast seawater. The experimental results are as follow The latent time of SCC occurrence gets longer as the initial stress intensity factor($K_{Ii}$) gets lower The main crack was propagated as the initial stress intensity factor($K_{Ii}$) gets higher, and secondary cracks occurred by electro-chemical factor a(ter stage of released stress. Dezincification phase showed around the crack, and the range of dezincification gets wider as the initial stress intensity factor($K_{Ii}$) gets higher.