• Title/Summary/Keyword: bees

Search Result 168, Processing Time 0.021 seconds

Antigastritic and Antiulcer action of Effective Compounds from Propolis Extract (Propolis 유효성분의 항위염 및 항위궤양 작용)

  • 김은주;정춘식;정기화
    • Biomolecules & Therapeutics
    • /
    • v.7 no.4
    • /
    • pp.362-370
    • /
    • 1999
  • Propolis, a natural resinous compound collected from honey bees, contains many biochemical constituents(wax, flavonoids, phenolic compounds, etc.) and has been used in traditional medicines as early as 300 B.C. It was been demonstrated that ethanol, acetylsalicylic acid, ischemia reperfusion, non-steroidal antiin-flammatory drugs and stress induce gastric lesions by promoting the generation of reactive oxygen metabolites. Therefore, some drugs that are capable of scavenging or inhibiting the generation of reactive oxygen radicals might be expected to prevent the gastric mucosal injury. The aim of this study was 1) to examine the antiulcer effect of propolis, 2) to investigate the mechanism of action by determining gastric acid secretion, lipid per-oxidation, mucus content and proton pump ($H^+$/$K^+$-ATPase) activity on gastric mucus in varios experimental models, and finally, 3) to isolate and identify the pure compounds that exert antiulcer activity. Step 2-1 and 2-3 sub-sub fraction shoed a significant reduction of severity of gastirc damage at the dose of 25 mg/kg in various experimental models. We isolated 4 sub-sub-sub fractions by flash column chromatography of Step 2-1 sub-sub fraction and one sub-sub-sub fraction by recrystalization of Step 2-3 sub-sub fraction. The protective effects of propolis sub-sub-sub fraction manifested sifnificant effects in HCl-ethanol induced gastric erosion model and aspirin induced gastric ulcer model. These results showed that the gastric mucosal protective effect of propolis might result from the increase of mucus secretion, free radical scavenging effect as well as the reduction of acid secretion in accordance with the reduction of $H^+$/$K^+$-ATPase activitv. Three compounds were isolated and identified from sub-sub fraction of propolis which showed antiulcer effects. Subsequently, these compounds were identified as a flavonoid, namely, 2-acetoxy-5,7,-dihydroxy-flavanone, galangin and chrysin.

  • PDF

The Influence of Insect Pollination and Artificial Pollination on Fruit Quality and Economic Profit in the 'Niitaka' Pear (Pyrus pyrifolia Nakai) (화분매개곤충과 인공수분이 '신고' 배의 과실품질과 수익성에 미치는 영향)

  • Lee, Kyeong-Yong;Yim, Sun-Hee;Seo, Ho-Jin;Kim, Sun-Young;Yoon, Hyung-Joo
    • Korean Journal of Organic Agriculture
    • /
    • v.24 no.4
    • /
    • pp.759-771
    • /
    • 2016
  • We compared the fruit set and the quality of the 'Niitaka' pear (Pyrus pyrifolia Nakai) among flowers pollinated by two bee species (Apis mellifera and Bombus terrestris) and pollinated artificial. The artificial pollination rate was 1.3 to 1.9 times higher than the bee pollination rate. Moreover, the artificially pollinated flowers produced fruit that was 5 to 10% higher in weight, 2 to 3% larger in size, and had a higher fruit shape index (L/D) than fruit pollinated by the bees. On economic analysis, net profit from insect pollinator was 93.5 to 97.1% of net profit from artificial pollination. Therefore, artificial pollination is more efficient than bee pollination in 'Niitaka' pear. However, regarding fruit quality and net profit, these results suggest that bee pollination can be an good alternative to artificial pollination in 'Niitaka' pear.

Intron sequence diversity of the asian cavity-nesting honey bee, Apis cerana (Hymenoptera: Apidae)

  • Wang, Ah Rha;Jeong, Su Yeon;Jeong, Jun Seong;Kim, Seong Ryul;Choi, Yong Soo;Kim, Iksoo
    • International Journal of Industrial Entomology and Biomaterials
    • /
    • v.31 no.2
    • /
    • pp.62-69
    • /
    • 2015
  • The Asian cavity-nesting honeybee, Apis cerana (Hymenoptera: Apidae), has been extensively studied for its biogeography and genetic diversity, but the molecules utilized in past studies were mainly ~90 bp long mitochondrial non-coding sequences, located between $tRNA^{Leu}$ and COII. Thus, additional molecular markers may enrich our understanding of the biogeography and genetic diversity of this valuable bee species. In this study, we reviewed the public genome database to find introns of cDNA sequences, with the assumption that these introns may have less evolutionary constraints. The six introns selected were subjected to preliminary tests. Thereafter, two introns, titled White gene and MRJP9 gene, were selected. Sequencing of 552 clones from 184 individual bees showed a total of 222 and 141 sequence types in the White gene and MRJP9 gene introns, respectively. The sequence divergence ranged from 0.6% to 7.9% and from 0.26% to 17.6% in the White gene and the MRJP9 introns, respectively, indicating higher sequence divergence in both introns. Analysis of population genetic diversity for 16 populations originating from Korea, China, Vietnam, and Thailand shows that nucleotide diversity (π) ranges from 0.003117 to 0.025837 and from 0.016541 to 0.052468 in the White gene and MRJP9 introns, respectively. The highest π was found in a Vietnamese population for both intron sequences, whereas the nine Korean populations showed moderate to low sequence divergence. Considering the variability and diversity, these intron sequences can be useful as non-mitochondrial DNA-based molecular markers for future studies of population genetics.

Effects of Propolis Extract on Antigastritic and Antiulcer (위염 및 위궤양에 미치는 Propolis 추출물의 영향)

  • 김은주;정기화;정춘식
    • Journal of Food Hygiene and Safety
    • /
    • v.14 no.4
    • /
    • pp.358-364
    • /
    • 1999
  • Propolis, a natural resinous compound collected from honey bees, contains many biochemical constituents and has been used in traditional medicines as early as 300 B.C. Recently, it has been reported to possess many biological activities such as antibacterial, antiviral, fungicidal, local anaesthetic, immunostimulating, antiinflammatory and free radical scavenging properties. This study was performed to investigate the pharmacological effects of the propolis extract and fractions on the gastric lesion and ulcer. The ethanol extract was fractionated with hexane, toluene and ethyl acetate. Followed by bioassay on antigastric and antiulcer activity. Propolis ethanol extracts(500, 750, 1,000, 1,500 and 2,000 mg/kg) showed the protective effect on HCl·ethanol-induced gastric lesion and the antisecretory effect against Shay’s gastric secretion in pylorus-ligated rats in a dose related manner. In the animal models of HCl·ethanol, aspirin-induced gastric lesion and Shay’s gastric secretion, the hexane and toluene fraction of propolis significantly reduced the length of gastric lesion and the acid secretion. These data showed that the gastric protective effects of propolis might result from reduction of acid secretion through the inactivation of H+/K+ATPase activity.

  • PDF

MOBA based design of FOPID-SSSC for load frequency control of interconnected multi-area power systems

  • Falehi, Ali Darvish
    • Smart Structures and Systems
    • /
    • v.22 no.1
    • /
    • pp.81-94
    • /
    • 2018
  • Automatic Generation Control (AGC) has functionally controlled the interchange power flow in order to suppress the dynamic oscillations of frequency and tie-line power deviations as a perturbation occurs in the interconnected multi-area power system. Furthermore, Flexible AC Transmission Systems (FACTS) can effectively assist AGC to more enhance the dynamic stability of power system. So, Static Synchronous Series Compensator (SSSC), one of the well-known FACTS devices, is here applied to accurately control and regulate the load frequency of multi-area multi-source interconnected power system. The research and efforts made in this regard have caused to introduce the Fractional Order Proportional Integral Derivative (FOPID) based SSSC, to alleviate both the most significant issues in multi-area interconnected power systems i.e., frequency and tie-line power deviations. Due to multi-objective nature of aforementioned problem, suppression of the frequency and tie-line power deviations is formularized in the form of a multi-object problem. Considering the high performance of Multi Objective Bees Algorithm (MOBA) in solution of the non-linear objectives, it has been utilized to appropriately unravel the optimization problem. To verify and validate the dynamic performance of self-defined FOPID-SSSC, it has been thoroughly evaluated in three different multi-area interconnected power systems. Meanwhile, the dynamic performance of FOPID-SSSC has been accurately compared with a conventional controller based SSSC while the power systems are affected by different Step Load Perturbations (SLPs). Eventually, the simulation results of all three power systems have transparently demonstrated the dynamic performance of FOPID-SSSC to significantly suppress the frequency and tie-line power deviations as compared to conventional controller based SSSC.

Honey Bee Venom (Apis mellifera) Contains Anticoagulation Factors and Increases the Blood-clotting Time

  • Zolfagharian, Hossein;Mohajeri, Mohammad;Babaie, Mahdi
    • Journal of Pharmacopuncture
    • /
    • v.18 no.4
    • /
    • pp.7-11
    • /
    • 2015
  • Objectives: Bee venom (BV) is a complex mixture of proteins and contains proteins such as phospholipase and melittin, which have an effect on blood clotting and blood clots. The mechanism of action of honey bee venom (HBV, Apis mellifera) on human plasma proteins and its anti-thrombotic effect were studied. The purpose of this study was to investigate the anti-coagulation effect of BV and its effects on blood coagulation and purification. Methods: Crude venom obtained from Apis mellifera was selected. The anti-coagulation factor of the crude venom from this species was purified by using gel filtration chromatography (sephadex G-50), and the molecular weights of the anti-coagulants in this venom estimated by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Blood samples were obtained from 10 rabbits, and the prothrombin time (PT) and the partial thromboplastin time (PTT) tests were conducted. The approximate lethal dose (LD) values of BV were determined. Results: Crude BV increased the blood clotting time. For BV concentrations from 1 to 4 mg/mL, clotting was not observed even at more than 300 seconds, standard deviations $(SDs)={\pm}0.71$; however, clotting was observed in the control group 13.8 s, $SDs={\pm}0.52$. Thus, BV can be considered as containing anti-coagulation factors. Crude BV is composed 4 protein bands with molecular weights of 3, 15, 20 and 41 kilodalton (kDa), respectively. The $LD_{50}$ of the crude BV was found to be $177.8{\mu}g/mouse$. Conclusion: BV contains anti-coagulation factors. The fraction extracted from the Iranian bees contains proteins that are similar to anti-coagulation proteins, such as phospholipase $A_2(PLA_2)$ and melittin, and that can increase the blood clotting times in vitro.

A Study on Characteristics of Building, Outdoor Playground & Equipment in Child Care Center (어린이집 건물, 실외놀이터 및 설비 특성에 관한 실태조사)

  • Byun, Hea-Ryung;Choi, Mock-Wha;So, Kab-Soo
    • Journal of the Korean housing association
    • /
    • v.24 no.6
    • /
    • pp.9-18
    • /
    • 2013
  • Recently, interest and social expectation for children's environment are on the rise. The needs for construction of safe and pleasant child care environment has bees agreed with. Especially, as the number of children who are wanted to go center jumped with additional support the government for child care business, centers have also seen a boost. But as number of child care centers increases rapidly in short period, the quality of centers is more inferior to before. and the space and facility level of many centers don't reach in legal minimum standard. In addition to, when child care centers are reviewed, it tend to be judged by one standard without considering connection with sense of values about child care or philosophy, child's traits, surrounding environment etc. Therefore, it is important to construct environment that can consider children's physical development and behavioral characteristic as well as basic element such thing as protection and support activity for current child care centers. This study presupposes that physical environment of child care centers makes important effect on teacher's motion and child's development, then it is to investigate it's characteristics in physically space, facilities, outdoor playground. And It is to identify space and accommodations, outdoors facilities which can support activity smoothly inside and outside. As a result, it is try to offer appliable basic information about the child-friendly environment of child care centers.

Effect of the Ethanol Extract of Propolis on Formation of Streptococcus mutans Biofilm

  • Park, Bog-Im;Jung, Yeon-Woo;Kim, Young-Hoi;Lee, Sang-Moo;Kwon, Lee-Seong;Kim, Kang-Ju;An, So-Youn;Choi, Na-Young;You, Yong-Ouk
    • International Journal of Oral Biology
    • /
    • v.41 no.4
    • /
    • pp.253-262
    • /
    • 2016
  • Streptococcus mutans (S. mutans) is one of the most important bacteria in the formation of dental plaque and dental caries. S. mutans adheres to an acquired pellicle formed on the tooth surface, and aggregates with many oral bacteria. It initiates plaque formation by synthesizing glucan from sucrose, which is catalyzed by glucosyltransferases. Propolis is a resinous mixture produced by honeybees, by mixing saliva and beeswax with secretions gathered from wood sap and flower pollen. Bees prevent pathogenic invasions by coating the propolis to the outer and inner surface of the honeycomb. Propolis has traditionally been used for the treatment of allergic rhinitis, asthma and dermatitis. We investigated the inhibitory effects of propolis ethanol extract on biofilm formation and gene expression of S. mutans. The biofilm formation of S. mutans was determined by scanning electron microscopy (SEM) and safranin staining. We observed that the extract of propolis had an inhibitory effect on the formation of S. mutans biofilms at concentrations higher than 0.2 mg/ml. Real-time PCR analysis showed that the gene expression of biofilm formation, such as gbpB, spaP, brpA, relA and vicR of S. mutans, was significantly decreased in a dose dependent manner. The ethanol extract of propolis showed concentration dependent growth inhibition of S. mutans, and significant inhibition of acid production at concentrations of 0.025, 0.05, 0.1 and 0.2 mg/ml, compared to the control group. These results suggest that the ethanol extract of propolis inhibits gene expression related to biofilm formation in S. mutans.

A Study on Expressive Features of Embroidered Norigae in the Chosun Dynasty (조선시대 수노리개에 나타난 표현 특성에 관한 연구)

  • Yang, Suk-Hyang
    • The Korean Journal of Community Living Science
    • /
    • v.22 no.1
    • /
    • pp.103-113
    • /
    • 2011
  • The purpose of the study was to examine formative features of embroidered norigae in form, color, pattern and expressive technique through positive analysis of relics and various of collections of work and to consider expressive features of embroidered norigae. The results of the study were as follows. First, embroidered norigae has handcrafted decoration. it was made by being sewed for women longing for their family's happiness. Embroidered norigae is a dress worn by women that is hung on a coat string or the waist part of a skirt. Second, it is eco-environmental. Embroidered norigae applied things seen in nature such as flowers, butterflies and bees to its pattern. Third, it has practicality. Embroidered norigae has high practical value besides a decorative function. Needle case norigae and incense case norigae provide functions in accordance with women's wisdom and skill as well as practicality. Fourth, it is praying for good luck. Women embroidered patterns symbolizing their desires in life such as their family's happiness, wealth, many sons and a long life. Fifth, it has balance and harmony. The knot of embroidered norigae has a perfect symmetry in the front/back part and in the right/left part. And the main body and tassel are symetrical in the right/left part, which gives stability and comfortableness. Embroidered norigae is classified into knot, main body and decorative part in its form. The three kinds express their unique beauty by being harmonized together. Finally, it has a property of melody. Movement of the tassel has a property of melody shaken by the wind and movement of its wearer.

The minimum inhibitory concentration (MIC) of bee venom against bacteria isolated from pigs and chickens (돼지 및 닭에서 분리된 균에 대한 봉독의 최소억제농도(MIC))

  • Kim Soon-Tae;Hwang Ji-Young;Sung Myoung-Suk;Je So-Yang;Bae Dong-Rok;Han Sang-Mi;Lee Sung-Hae
    • Korean Journal of Veterinary Service
    • /
    • v.29 no.1
    • /
    • pp.19-26
    • /
    • 2006
  • Bee venom is synthesized in the venom gland's of worker and queen bees and stored in their venom sacs. Bee venom is a rich source of enzymes, peptides and biogenic amines. there are at least 18 active components in the venom which have some pharmaceutical properties. This study was performed to evaluate minimum inhibitory concentration(MIC) of bee venom against bacteria isolated from pjgs and chickens with disease. In case of reference strains, the MIC $({\mu}g/m{\ell})$ of Staphylococcus aureus ATCC 6538, Streptococcus mutans ATCC 25175, and Salmonella typhimurium ATCC 6538 were 64, 64 and 32, respectively. In case of bacteria isolated from pig and chicken, the MIC of Staphylococcus aureus, Staphylococcus hyicus and Staphylococcus chromogenes were 8, 128 and 128, and that of 11 strains of Escherichia coli were 8 to >512 and that of 8 strains of Salmonella sup were >512. Antibacterial resistance test of 22 strains isolated from pig and chicken and 3 reference strains were performed by agar gel diffusion method, using 17 antibacterial drugs including penicillin, cefazolin, tetracycline and quinolone group. The multiple drug resistant patterns were found in most strains isolated from pig and chicken.