• 한국임상수의학회지
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• 제12권1호
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• pp.887-896
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• 1995

In order to determine the efficacy of $Bayvarol^{(R)}$ strips(Bayer AG) against the mite Varroa jacobsoni and the safety of $Bayvarol^{(R)}$ strips in honey bees, a series of trials was carried out under field condition in autumn and spring. The results obtained were as follows; 1. The average varroacidal rate of $Bayvarol^{(R)}$ strips was 92.7% in autumn and 93.7% in spring. However the average varroacidal rats of treated control group was 73.8 % in autumn and 75.1 % in spring, and that of untreated control group was 12.1 % in autumn and 16.1 % in spring. 3. The average number of dead bees in $Bayvarol^{(R)}$ group was 13.0 in recommended dosage(RD), 12.3 in 2 times of RD and 14.0 in 4 times of 70 in autumn, and 19.7 in RD,17.0 in 2 times of RD and 19.0 in 4 times of RD in spring. However the average number of dead bees in treated control groups was 38.3 in RD, 62.7 in 2 times of RD and 60.3 in 4 times of RD in autumn and 18.0 in RD, 43.3 in 2 times of RD and 102 in 4 times of RD in spring and that of untreated control of group was 12.7 in autumn and 19.3 in spring. 3. The varroacidal effects and the number of dead bees of all groups were higher in spring than in autumn. 4. It was revealed that $Bayvarol^{(R)}$ were highly saft and effective varroacides for honey bees.

• 한국환경생태학회지
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• 제33권6호
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• pp.654-663
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• 2019

수분생태학(pollination ecology)의 주체인 화분매개곤충은 농업, 생물산업에 있어서 작물생산에 대한 경제적 파급효과에 대해서 매우 가치가 있는 곤충으로 인정되어 왔다. 생산되는 주요 작물의 약 70% 이상이 화분매개곤충의 수분(pollination)매개활동에 의해 생산되는 것과 같이, 화분매개곤충의 존재는 인류에게 있어서 매우 중요하다고 볼 수 있다. 그러나 화분매개곤충은 오랜 기간동안 변화하는 기후영향과 토지의 이용, 지형의 변화, 농작물 생산량 증대를 위한 농약 사용 등으로 인해 위협을 받고 있고 그 수가 매년 점차적으로 줄어들고 있어서 종 다양성에 대한 위험적 변화가 예상된다. 따라서 기후의 변화, 지형의 변화, 인간의 간섭에 따른 토지의 이용 등의 다양한 위험적인 요인들로부터 수분생태계 종 다양성 보존과 관리를 위하여 수분을 매개하는 야생벌의 분포에 대한 조사와 분석은 매우 중요하다. 본 연구는 2017년에서 2018년까지 전국 51개 조사지점에서 2주~한달 간격으로 말레이즈 트랩(ez-Malaise trap)을 사용하여 전국단위에서 출현하는 야생벌을 시기별, 공간별로 야생벌을 채집하였고, 채집된 꿀벌상과, 청벌상과 및 말벌상과를 대상으로 공간별, 시기별 출현현황을 분석하였다. 시기별로 전국단위로 출현한 야생벌은 정규분포 형태를 보이며 7월과 8월 사이에 크게 개체수가 증가하고 11월까지 출현하는 것을 확인하였다. 공간적으로도 야생벌은 위도에 따라서 출현분포의 차이를 유의적 수준에서 확인하였으나, 경도에 대해서는 유의적 차이를 확인할 수 없었다. 그러나 기후적 요인, 지형적 특성, 인간간섭에 따른 토지의 활용 등을 종합적으로 고려할 경우에 야생벌의 분포차이를 더욱 면밀히 분석할 수 있을 것으로 판단되며 추후의 연구로써 진행될 수 있을 것이다. 본 연구를 통한 전국에 시기별, 공간별로 출현하는 야생벌의 분포를 통해서 향후 농업발전을 위한 화분매개관련 곤충 연구에서부터 나아가 수분생태계에서의 야생벌 종 다양성 보전 및 관리를 위한 기초적 자료로 활용될 수 있을 것으로 기대된다.

• Current Research on Agriculture and Life Sciences
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• 제32권2호
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• pp.63-66
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• 2014

The effects of a newly developed flower thinning formulation (FTF) on the vitality of the honey bee Apis mellifera were examined by measuring the activities of various digestive enzymes in adult worker bees. First, direct spraying of the FTF solution did not cause any behavioral changes or lethal effects for the honey bees based on 24 h observation. Second, oral ingestion of a sugar solution containing the FTF did not produce any significant change in the activities of amylase, proteinases, lipase, or acetylcholine esterase (AChE) in the worker bees 6 h or 24 h after treatment. Meanwhile, a commercial formulation containing sulfur compounds showed slightly reduced activities for several digestive enzymes and AChE, although no behavioral disturbance. Thus, the results of the present study suggest that the FTF is not toxic for honey bees, in terms of contact and ingestion. Therefore, this newly developed FTF can be used for flower thinning without any detrimental effects on pollinating insects.

• Mycobiology
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• 제45권3호
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• pp.204-208
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• 2017

Nosema ceranae is an obligate intracellular fungal parasite that causes mortality in honey bees and enhances the susceptibility of honey bees to other pathogens. Efficient purification of Nosema spores from the midgut of infected honey bees is very important because Nosema is non-culturable and only seasonably available. To achieve a higher yield of spores from honey bees, in this study, we considered that the initial release of spores from the midgut tissues was the most critical step. The use of 2 mm beads along with enzymatic treatment with collagenase and trypsin enhanced the homogenization of tissues and the yield of released spores by approximately 2.95 times compared with the use of common 3 mm beads alone. The optimal time for the enzyme treatment was determined to be 1 hr as measured by the yield and viability of the spores. A one-step filtration using a filter paper with an $8-11{\mu}m$ pore size was sufficient for removing cell debris. This method may be useful to purify not only N. ceranae spores but also other Nosema spp. spores.

• Earthquakes and Structures
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• 제16권2호
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• pp.165-175
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• 2019

Incremental dynamic analysis (IDA), as an accurate method to evaluate the parameters of structural performance levels, requires many non-linear time history analyses, using a set of ground motion records which are scaled to different intensity levels. Therefore, this method is very computationally demanding. In this study, a new method is presented to estimate the summarized (16%, 50%, and 84% fractiles) IDA curves of a first-mode dominated structure using discrete wavelet transform and bees optimization algorithm. This method reduces the number of required ground motion records for the prediction of the summarized IDA curves. At first, a subset of first list ground motion records is decomposed by means of discrete wavelet transform which have a low dispersion estimating the summarized IDA curves of equivalent SDOF system of the main structure. Then, the bees algorithm optimizes a series of factors for each level of detail coefficients in discrete wavelet transform. The applied factors change the frequency content of original ground motion records which the generated ground motions records can be utilized to reliably estimate the summarized IDA curves of the main structure. At the end, to evaluate the efficiency of the proposed method, the seismic behavior of a typical 3-story special steel moment frame, subjected to a set of twenty ground motion records is compared with this method.

• 식품보건융합연구
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• 제5권1호
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• pp.33-38
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• 2019

The purpose of previous study centered around introducing minerals into bee colonies was to observe the Varrosa destructor mites-repelling effect of minerals ("Pszczelarstwo" 6/2016). The results of research published so far confirmed the purpose of using minerals in the fight against Varrosa infestation. This publication presents partial results of the next stage of research. Using foundation enriched with studied minerals in right proportions leads to diminishing the number of Varroa destructor parasites in bee colonies. It may also contribute to increase in the amount of honey obtained from bees. Foundations with minerals were in most cases correctly filled with larvae, and the new generation of bees didn't show any morphometric or physical-motor aberrations. The effect of minerals in the foundation weakens with time, which requires adequate reaction from the beekeeper. The study showed no negative effect of mineral suspensions added directly to royal jelly of larvae in the swarm cells and worker cells on the development of bees. Additional research was carried out in August and September and led to surprising observations, which are still too early to report. It obliges us to carry out further, extended subject research on a large number of bee colonies in 2017.

• Journal of Ecology and Environment
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• 제46권1호
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• pp.62-75
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• 2022

Background: Pollinators help plants to reproduce and support economically valuable food for humans and entire ecosystems. However, declines of pollinators along with population growth and increasing agricultural activities hamper this mutual interaction. Nectar and pollen are the major reward for pollinators and flower morphology and volatiles mediate the specialized plant-pollinator interactions. Limited information is available on the volatile profiles attractive to honey bees and bumblebees. In this study we analyzed the volatile organic compounds of the flowers of 9 different plant species that are predominantly visited by honey bees and bumblebees. The chemical compositions of the volatiles were determined using a head space gas chromatography-mass spectrometry (GC-MS) method, designed to understand the plant-pollinator chemical interaction. Results: Results showed the monoterpene 1,3,6-octatriene, 3,7-dimethyl-, (E) (E-𝞫-ocimene) was the dominating compound in most flowers analyzed, e.g., in proportion of 60.3% in Lonicera japonica, 48.8% in Diospyros lotus, 38.4% Amorpha fruticosa and 23.7% in Robinia pseudoacacia. Ailanthus altissima exhibited other monoterpenes such as 3,7-dimethyl-1,6-octadien-3-ol (𝞫-linalool) (39.1%) and (5E)-3,5-dimethylocta-1,5,7-trien-3-ol (hotrienol) (32.1%) as predominant compounds. Nitrogen containing volatile organic compounds (VOCs) were occurring principally in Corydalis speciosa; 1H-pyrrole, 2,3-dimethyl- (50.0%) and pyrimidine, 2-methyl- (40.2%), and in Diospyros kaki; 1-triazene, 3,3-dimethyl-1-phenyl (40.5%). Ligustrum obtusifolium flower scent contains isopropoxycarbamic acid, ethyl ester (21.1%) and n-octane (13.4%) as major compounds. In Castanea crenata the preeminent compound is 1-phenylethanone (acetophenone) (46.7%). Conclusions: Olfactory cues are important for pollinators to locate their floral resources. Based on our results we conclude monoterpenes might be used as major chemical mediators attractive to both honey bees and bumblebees to their host flowers. However, the mode of action of these chemicals and possible synergistic effects for olfaction need further investigation.

• 한국방재안전학회논문집
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• 제16권4호
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• pp.85-99
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• 2023

현대사회가 고도화되고, 후쿠시마 원전사고 역시 자연재난의 연쇄적인 영향으로 인해 발생함에 따라 복합재난을 고려한 방재계획의 중요성이 나날이 커지고 있다. 그러나 현재 국내의 방사능방재 분야에서 복합재난을 고려한 구체적인 방재계획은 제시되지 않고 있다. 따라서, 본 연구에서는 복합재난의 유형을 분석하여 국내 환경에서 비교적 발생 가능성이 높은 유형을 선정하고, 자연재난 유형별 피해확산이 방사능방재에 미치는 영향을 분석하고자 한다. 복합재난을 고려한 방사능방재 시나리오를 통해 방재계획 수립에 필요한 기준들을 제시하였고, 이러한 기준은 최악의 상황 대비에 활용한다. 이는 실효적인 주민보호조치를 결정하고 이행하는 데에 도움을 줄 수 있을 것으로 판단된다.

• 대한수의학회지
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• 제16권1호
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• pp.11-25
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• 1976

Studies on pathogenicities and developmental stages of Nosema apis (Zander, 1909) were carried out through artificial infection to Nosema free honey bees with various levels of spores isolated from local honey bee colony. The results obtained were summarized as follows: 1. The clinical symptoms were observed as dysentery, enteritis of mid-gut (enlargement and decoloration), crawling posture and shortening of the longevity of worker bees in the rearing honey bee colony inoculated with the spores. 2. Number of spores harvested from laboratory rearing honey bees were progresively increased to 4 weeks after inoculation. The regression equations and coefficients of correlations to various spore levels were as follows in each treatment colony. Colony 1. ($$1,000{\times}10^4spores/ml$$) $$y_{c1}=471{\times}10^{4}x+454{\times}10^4(r=0.65^*$$) Colony 2. ($$500{\times}10^4spores/ml$$) $$y_{c2}=340{\times}10^{4}x+207.8{\times}10^4(r=0.99^{**}$$) Colony 3. ($$100{\times}10^4spores/ml$$) $$y_{c3}=150{\times}10^{4}x+84.2{\times}10^4(r=0.99^{**}$$) Colony 4. ($$10{\times}10^4spores/ml$$) $$y_{c4}=13.8{\times}10^{4}x+13{\times}10^4(r=0.98^{**}$$) 3. Average longevity of worker bees artificially infected with Nosema apis was shortened as 21.7~43.8% compare to the control. (p<.05, p<.01) 4. The spores which were isolated from honey bee colony infected with Nosema disease were ovoid or spherical form, and measured, as a rule, from $4.7{\mu}m$ to $6.1{\mu}m$ (mean $5.3{\mu}m$) in length and from $2.4{\mu}m$ to $3.2{\mu}m$ (mean $2.9{\mu}m$) in width. 5. In the mid-gut of honey bees, the spore was progresively germinated and became trophozoite stage. The trophozoites were grown to meronts and their binary fission were begun. The divided two sporoblasts were developed to the spores which had elastic membrane. The new spores were shed in excreta of honey bees 10~15 day after inoculation at $25{\pm}2$ centigrade. 6. The ultrastructure of spore membrane consisted of three layers, such as, outer, middle and inner layer. The sporoplasm consisting lamellar structure occupied only anterior part of the spore and was often extended to posterior direction where definite vacuoles and a polar filament was able to detect.

• 한국응용곤충학회지
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• 제41권4호
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• pp.263-267
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• 2002

사과원에서 꿀벌 대신에 화분매개곤충으로 유망한 뿔가위벌류의 활용을 목적으로, 1996년부터 1998년까지 각각 전국 66, 52, 60개소 산지에 내경 6-8mm인 대나무대롱을 설치하여, 자생하고 있는 뿔가위벌류의 종별 분포와 채집량을 조사하였다. 머리뿔가위벌, 뿔가위벌, 붉은뿔가위벌, 외뿔가위벌, 꼬마민뿔가위벌의 5종이 채집되었으며, 각종별 점유율은 각기 39.9-51.7, 28.3-53.7, 5.5-19.1, 0.8-0.9, 0-0.1%였다. 매년 머리뿔가위벌과 뿔가위벌 2종이 전체 채집량의 80%내외였으며, 채집량이 많은 5개지역중 원주지역에서 머리뿔가위벌이 우점종이었고, 나머지 4개지역(안성, 괴산, 봉화, 구미)에서는 뿔가위벌이 우점종이었다. 암컷의 비율은 머리뿔가위벌 29-43, 뿔가위벌 10-12, 붉은뿔가위벌 22-43%로 종간에 차이가 있었다. 3종 뿔가위벌류는 채집지역의 해발을 200m 이하, 300-400m, 500m 이상으로 구분하였을 때, 해발이 높아짐에 따라서 채집수가 많았고, 3종 뿔가위벌류중 머리뿔가위벌의 점유율이 높은 경향이었다. 3년간 동일장소에서 연속 채집할 경우 머리뿔가위벌과 붉은뿔가위벌은 연도가 경과할수록 채집량이 점차로 증가하는 경향이었으나, 뿔가위벌은 반대로 크게 감소하였다. 3종 뿔가위벌류가 대나무 대롱에 영소하는 시기는 3월 하순-4월 중순에 시작하여 5월 하순-6월 중순에 종료하였으며, 영소 최성기는 4월 하순-5월 중순이었다. 한편. 뿔가위벌류의 경쟁종인 구멍벌은 5월 하순-6월 중순에 시작하여 6월 하순-7월 중순에 가장 많이 영소하였고, 감탕벌은 4월 하순5월 중순에 시작하여 7월 하순8월 중순에 가장 많이 영소하였다.