• Title/Summary/Keyword: bees

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A Study on the Method for Removing the Paraffin used on Iron Artifacts as Surface Coating Agent - As Focused on the Iron Artifacts Owned by the Kyunghee University Central Museum - (철제유물 표면코팅제로 사용된 파라핀 제거방법에 관한 연구 - 경희대학교박물관 소장 철제유물을 중심으로 -)

  • Kang, Seokin;Wi, Koangchul;Lee, Hoyeon;Lim, Seongjin
    • Conservation Science in Museum
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    • v.13
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    • pp.23-31
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    • 2012
  • The object artifacts of this study are the iron artifacts owned by the Kyunghee University Central Museum. The surfaces of the iron artifacts are opaque due to the coated materials which are presumed to be paraffin or bee's wax while they are plate-shaped and exist in thickly exfoliated condition caused by severe corrosion developed on the overall surfaces. Therefore, in order to remove the coated materials away from the surfaces of the artifacts rather safely, reversibility tests have been carried out and the conservation treatment was performed upon the basis of and in application of the experimental results. The study methodologies are that: first, the FT-IR analysis was carried out to determine the ingredients of the coating-treated material on the surface; second, by applying various kinds of organic solvents, the kinds of agents that allow the artifact's surface-coating material to be reversible have been identified through experiments; third, the most suitable agent for removing coating material was selected and applied to the real artifacts on the basis of the results of the reversibility tests. Results from the study: first, as a result of the FT-IR analysis, the coating agent used on the artifacts was identified to be of the same ingredients as those of paraffin; second, among organic solvents, xylene, toluene, trichloroethylene and methyl alcohol were identified to be usable for dissolving paraffin whereas toluene was judged to be the most suitable for removing the coating agent; third, when applying the selected agents on the real artifacts, due to the fact that removing the whole paraffin might cause the artifact to disintegrate, the paraffin of only the part that covered the surfaces opaquely was removed using cotton swab or gauze, thus completing the conservation treatment.

Changes in Lymphocyte DNA Damage and Antioxidant Status after Supplementing Propolis to Korean Smokers: A Placebo-Controlled, Double-Blind Cross-Over Trial (프로폴리스 섭취 후 흡연자의 임파구 DNA 손상도 및 항산화 상태의 변화: 이중맹검 교차 인체시험)

  • Kang, Myung-Hee;Lee, Hye-Jin;Kim, Mi-Kyung;Sung, Mi-Kyung;Kwon, O-Ran;Park, Yoo-Kyoung
    • Journal of Nutrition and Health
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    • v.42 no.5
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    • pp.442-452
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    • 2009
  • Smoking has been known to exacerbate the initiation and propagation of oxidative stresses. Efforts have been made to reduce the smoking-induced oxidative stresses using commercial dietary supplements. Propolis is the resinous substance collected by bees from the leaf buds and bark of trees, especially poplar and conifer trees. In this trial, we examined whether a daily supplementation of 800 mg propolis can protect endogenous lymphocytic DNA damage and modulate antioxidative enzyme activities and the level of antioxidant vitamin in smokers using a placebo-controlled, doubleblinded cross-over trial. After two weeks of running-in period, 29 smokers (mean age 34.38 ${\pm}$ 1.73) received 6 tablets/day of either propolis or placebo pills for 4 weeks. After 2 weeks of washout period the subjects switched they pills for cross-over study. The degree of DNA damage (assessed by tail DNA, tail length and tail moment) was not significantly changed with propolis intake or placebo intake. Similarly, total antioxidant status (TAS) remained at the same level regardless of the treatment. Erythrocyte catalase, glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), plasma vitamin C and tocopherol level did not differ before and after propolis treatment, and did not differ between treatments. Putting all these results together, we would suggest that it is still too early to claim that propolis possess antioxidative activities.

Immunomodulatory Effects of Propolis and Fermented-propolis in BALB/c Mice (프로폴리스 및 발효 프로폴리스의 섭취가 BALB/c mice의 면역활성에 미치는 영향)

  • Kim, Yoon-Hee;Kwon, Hyuck-Se;Kim, Dae-Hwan;Park, IL-Hwan;Park, Sang-Jae;Shin, Hyun-Kyung;Kim, Jin-Kyung
    • Korean Journal of Food Science and Technology
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    • v.40 no.5
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    • pp.574-579
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    • 2008
  • Propolis is the generic term for the resinous substance collected by honey bees from a variety of plant sources. In this study, we have assessed the immunomodulatory properties of propolis (P) and fermented-propolis (FP) in BALB/c mice. Mice were subjected to gavage once a day (for 14 days) with 50, 100, 200 mg/kg body weight P, FP, or vehicle. Lymphocytes were isolated from the spleen and mesenteric lymph nodes (MLN) and the immune cell proportions, proliferative activities, and cytokine production were evaluated. The P- and FP-administration induced similar, but differential, alterations in the percentage of immune cell populations and their biological functions, including cytokine production and NK cell cytotoxicity. The proportion of$ CD4^+$ and $CD8^+$ T cells in the spleen was increased slightly in the P- and FP-administered mice as compared to the vehicle-treated mice. In MLN, the percentage of $CD4^+$ T cells was increased significantly in the 200 mg/kg P-treated mice. The mice which were treated with P and FP evidenced significantly increased interferon-$\gamma$ and interleukin-4 production in concanavalin A-stimulated splenocytes, whereas the production of theses cytokines was not shown to be induced by P-treatment. In addition, NK cell activity was also increased dramatically by the administration of P and FP. Collectively, these findings showed that P and FP are wide-spectrum immunomodulators, which may modulate both innate and adaptive immune responses.

Evaluation of Commercial Korean Honey Quality and Correlation Analysis of the Quality Parameters (국내 시판 벌꿀의 품질 평가 및 품질인자간 상관관계 분석)

  • Sung, Hwa-Jung;Jung, Chuleui;Kwon, Jiyoung;Sohn, Ho-Yong
    • Journal of Life Science
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    • v.28 no.12
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    • pp.1489-1500
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    • 2018
  • Honey is made from flower nectar by honey bees. In this study, 120 honeys from various flowers and across eight different provinces in Korea were collected and their components, antioxidants, and hemolytic activities against red blood cell were evaluated. Our results show that total polyphenol (TP) varied widely across the samples, with chestnut honey showing the highest TP ($77.1{\pm}8.4mg/100g$), protein content ($25.9{\pm}0.9mg/100g$), and absorbance at 400 nm ($A_{400}$ : $0.156{\pm}0.036$). In contrast, the acacia honey and sugar honey had a TP of 9.5~30 mg, 12~15 mg/100g of, and the lowest $A_{400}$ of $0.06{\pm}0.02$. High amounts of total flavonoid were quantified in the jujube and chestnut honeys at $8.73{\pm}7.31$ and $8.39{\pm}3.02mg/100g$, respectively. No samples demonstrated hemolytic activity up to 1 mg/ml. Antioxidant activities determined by DPPH, ABTS, and nitrite scavenging placed the chestnut honey highest, followed by jujube, styrax, multi-floral, citrus, acacia and sugar honey. Analysis of parameter correlations indicated that the components and bioactivity of the honey are dependent on the origin of the flower rather than on bee-farming regions. A positive correlation between TP content and antioxidant activity was identified. The correlation coefficients between $A_{400}$ and the TP, ABTS scavenging, and reducing power values were 0.804, 0.772 and 0.741, respectively. We therefore suggest that $A_{400}$ could be used as a noble, economic and simple factor for honey quality evaluation. Our results can potentially be used to develop functional honey for the food and pharmaceutical industries.

Determination of Fire Blight Susceptibility on Wild Rosaceae Plants in Korea by Artificial Inoculation (인공접종을 통한 국내 야생 장미과 식물의 화상병 감수성 검정)

  • In Woong Park;Yu-Rim Song;Eom-Ji Oh;Yoel Kim;In Sun Hwang;Mi-Jin Jeon;Chorong Ahn;Jin-Suk Kim;Soonok Kim;Chang-Sik Oh
    • Research in Plant Disease
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    • v.29 no.1
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    • pp.23-38
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    • 2023
  • The fire blight caused by Erwinia amylovora (Ea) is a devastating disease of Rosaceae plants, including commercially important apple and pear trees. Since the first report in Korea in May 2015, it has been spreading to neighboring regions gradually. Host plants can be infected by pollinators like bees, rainfall accompanied by wind, and cultural practices such as pruning. Many studies have revealed that wild Rosaceae plants such as Cotoneaster spp., Crataegus spp., Pyracantha spp., Prunus spp., and Sorbus spp. can be reservoirs of Ea in nature. However, wild Rosaceae plants in Korea have not been examined yet whether they are susceptible to fire blight. Therefore, the susceptibility to fire blight was examined with 25 species in 10 genera of wild Rosaceae plants, which were collected during 2020-2022, by artificial inoculation. Bacterial suspension (108 cfu/ml) of Ea type strain TS3128 was inoculated artificially in flowers, leaves, stems, and fruits of each plant species, and development of disease symptoms were monitored. Moreover, the presence of Ea bacteria from inoculated samples were checked by conventional polymerase chain reaction. Total 14 species of wild Rosaceae plants showed disease symptoms of fire blight, and Ea bacteria were detected inside of inoculated plant parts. These results suggest that wild Rosaceae plants growing nearby commercial apple and pear orchards in Korea can be Ea reservoirs, and thus they should be monitored regularly to minimize the damage by Ea infection and spreading.

The Influence of K-Content Experience on National Image, Tourism Attitude and Visit to Intention: Targeting Chinese (K-콘텐츠 경험이 국가이미지와 관광태도 및 방문의도에 미치는 영향 : 중국인을 대상으로)

  • Park, Heejung
    • Journal of Service Research and Studies
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    • v.14 no.1
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    • pp.91-107
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    • 2024
  • This study attempted to empirically verify the possibility of K-content for Chinese people who have recently slowed down due to restriction of movement and political and diplomatic conflicts, although it is a very meaningful market for Korea's content industry and tourism industry. As a result of the study, each item of K-content experience, national image, tourism attitude, and visit intention was derived as one factor, and only the national image factor was derived as two factors: 'functional image' and 'cultural image'. As a result of examining the influence relationship between them established based on previous studies focusing on the derived factors, all five research hypotheses were adopted. K-content experience was found to have a significant influence on both factors of the national image. It was found that it had a greater influence on cultural image factors than functional image factors, cultural image factors were found to have a greater influence on tourism attitudes, K-content experiences had a significant effect on tourism attitudes, and tourism attitudes had a significant effect on visit intentions. Based on the results of this study, it was once again confirmed that the national image even comtually bees an important factor for linking to practical tourism behavior, and in this respect, "culture" is an important key factor that can lead to practical tourism and visits. Previous national images indicate that if the functional aspect of the country was more emphasized, it is now necessary to focus more on the importance of culture than on the functional aspect. As the K-content experience has a significant effect on tourism attitude, it can have a positive effect on the formation of a positive tourism attitude that can lead to actual tourism behavior, so various efforts will be needed to form an active tourism attitude using K-content in the future. As the content and target scope of K-content are expanded and diversified, specific strategies for each sub-market using cultural contents in various fields should be established and implemented.