• Journal of Animal Science and Technology
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• 제47권2호
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• pp.293-304
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• 2005

This experiment was carried out to investigate effects of honeybee venom treatment on the body weight gain, feed conversion and growth rate' in pigs. One hundred twenty nine piglets(LY) from 12 sows were allocated into two groups; honeybee venom-treated group (66 piglets from 6 sows) and non-treated control group (63 piglets from 6 sows). Natural honeybee venom was administrated at 0, 3, 14, 30, and 74 days after birth. The acupoints were Hai-men(ST-25), Du-kou(CV-8) and liao-chao(GV-I) points at 0 day, the regions of castration and tail ampution at 3 days, liao-chao(GV-1) and Bai-hui(GV-20) points at 14(weaning), 30(move into piglet stall) and 74(move into grower stall) days after birth. Control group was injected 1 ml of saline to the same site. Average body weight at 150 days after birth was 98.44 kg in bee venom treated group and 86.24 kg in control group, respectively. Average body weight of treated group was significantly increased by 14.15%(P < 0.0001). Average daily gain of bee venom treated group and control group were 649 g and 569 g, respectively, increased by 14.06%(P< 0.0001). Feed conversion of the treated group was 2.21 and control group was 2.49, increased by 11.25% in control group(P < 0.001). Survival rate at weaning was 95.5 % in treated group and 92.0% in control group, at growing(74 days after birth) was 92.4% in treated group and 85.7% in control group and survival rate at finishing(l50 days after birth) was 89.5 % and 79.4 %, respectively. Collecting together, the results in this study showed that growth performance and feed conversion were increased by treatment of natural honeybee venom to pigs. These results suggested that the treatment of bee venom could be used effectively for the increase productivity of livestock industry.

• 생약학회지
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• 제40권3호
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• pp.173-177
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• 2009

The in vitro antibacterial activities of honeybee(Apis mellifera. L) venom collected by a bee venom collector were investigated against several bacteria including antibiotic-susceptible and resistant Propionibacterium acnes. Honeybee venom was prepared with different concentrations and they showed strong antibacterial activites. Honeybee venom inhibited the growth of the tested antibiotic-resistant P. acnes at the concentration of 1 mg/ml. The inhibitory activities of the honeybee venom showed time-dependent manner. Honeybee venom did not influence the viability of human dermal fibroblast at the high concentration of less than 10 mg/ml. From these results, we expect that honeybee venom has strong antibacterial activities and has advantage for treating cure.

• 대한약침학회지
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• 제19권3호
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• pp.225-230
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• 2016

Objectives: Mellitine, a major component of bee venom (BV, Apis mellifera), is more active against gram positive than gram negative bacteria. Moreover, BV has been reported to have multiple effects, including antibacterial, antivirus, and anti-inflammation effects, in various types of cells. In addition, wasp venom has been reported to have antibacterial properties. The aim of this study was to evaluate the antibacterial activity of BV against selected gram positive and gram negative bacterial strains of medical importance. Methods: This investigation was set up to evaluate the antibacterial activity of BV against six grams positive and gram negative bacteria, including Staphylococcus aureus (S. aureus), Salmonella typhimurium, Escherichia coli (E. coli) O157:H7, Pseudomonas aeruginosa, Burkholderia mallei and Burkholderia pseudomallei. Three concentrations of crude BV and standard antibiotic (gentamicin) disks as positive controls were tested by using the disc diffusion method. Results: BV was found to have a significant antibacterial effect against E. coli, S. aureus, and Salmonella typhyimurium in all three concentrations tested. However, BV had no noticeable effect on other tested bacteria for any of the three doses tested. Conclusion: The results of the current study indicate that BV inhibits the growth and survival of bacterial strains and that BV can be used as a complementary antimicrobial agent against pathogenic bacteria. BV lacked the effective proteins necessary for it to exhibit antibacterial activity for some specific strains while being very effective against other specific strains. Thus, one may conclude, that Apis mellifera venom may have a specific mechanism that allows it to have an antibacterial effect on certain susceptible bacteria, but that mechanism is not well understood.

• 한국임상수의학회지
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• 제15권2호
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• pp.247-250
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• 1998

This study was designed to examine therapeutic effect of honeybee venom on wound healing in tail-docked dog. Blood samples were taken of jugular vein and hematological values were analyzed in condition of pre,3- and 6-day after canine caudectomy. Apitherapy group was subcutaneously treated with an Italian honeybee(Apis mellifera ligustica). Antibiotic therapeutic group was intramuscularly iaiected with a standard dosage ol'penicluin ,G procaine(20,000 Units/kg of body weight). Changes of leucocyte, erynlrocytel hematocrit, platelet and fibrinogen were not significantly different between bee sting and penicillin injection during wound healing. No changes of wound healing in tine tests, groups were onseren. Whole honeybee venom has been shown to have a antiinflammatory effect in tail-docked dog. It may be concluded that honeybee venom is effective drug being useful far wound healing and disinfection without tissue trauma in dog.

• 한국식품위생안전성학회지
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• 제32권3호
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• pp.228-233
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• 2017

서양종 꿀벌의 일벌의 독을 채취하여 정제한 정제봉독에 대한 세균에서의 돌연변이 유발성 검색을 위하여 S. typhimurium의 히스티딘 요구성 균주 TA100, TA1535, TA98, 그리고 TA1537의 균주와 대장균 E. coli의 트립토판 요구성 균주인 WP2 uvrA를 이용해 복귀돌연변이시험을 실시하였다. 정제봉독은 부형제로 사용한 멸균생리식염수에 용해되었으며 모든 농도군의 조제물에서 침전은 관찰되지 않았으며, 정제봉독 조제물을 top agar와 혼합할 때 모든 농도군에서 혼탁이나 침전이 관찰되지 않았다. 대사활성계 적용 TA100, TA1535, TA98 균주에 대해 0, 1.5, 5, 15, 50, 150 및 500 µg/plate의 범위를 설정하고 미 적용시엔 0, 0.15, 0.5, 1.5, 5, 15 및 $500{\mu}g/plate$의 범위로 설정하였다. TA1537과 WP2 uvrA균주에 대한 농도 범위은 대사활성계 적용시엔 0, 5, 15, 50, 150, 500 및 1,500를 미 적용시엔 0, 1.5, 5, 15, 50, 150 및 500 µg/plate 범위로 설정하여 시험을 수행하였다. 그 결과 모든 시험균주에서 대사활성계 적용 여부에 상관없이 정제봉독 처리군의 평균 집락 수는 증가를 나타내지 않았으며, 양성판정 기준을 만족시키지 못하였다. 따라서, 정제봉독은 본 시험조건 하에 사용한 시험 균주에 복귀돌연변이를 유발하지 않는 것으로 사료되었다.

• 한국동물위생학회지
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• 제29권3호
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• pp.287-295
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• 2006

This study was conducted to evaluate effects of honeybee venom injection (VI) collected using bee venom collector compared to that of bee venom accupuncture (VA) on the body weight gain, growth rate and hematological characteristics of piglets. One hundred sixty two piglets from 15 sows were allocated in to three groups; honeybee venom subcutaneous injection groups (97 piglets from 9 sows), honeybee venom accupuncture -treated group (31 piglets from 3 sows), and non -treated control group and 30 days after birth. Honeybee venom subcutaneous injection groups divided by a syringeful; group A (0.5, 1.0, 1.5, 2.0 mg ), group B (1.0, 1.0, 1.0, 1.0 rug), and group C (1.0, 1.5, 2.0, 2.5 mg). During 60 days experiment, weight gain and survivability in VI and VA treatment of pigs were higher compared with control. Survival rate during the experiment period was 96.8% in group C, 93.2 % in VA and 86.7 % in control. Weight gain and survivability were effected by VI and VA. WBC, RBC, lymphocytes, monocytes, serum total protein, and albumin concentration were not affected by VI and VA. Serum IgG concentration of VI and VA treatments were greater than that of control. In conclusion, VI and VA were effective for improving growth performance and to increase the concentrations of blood Ig G in growing pigs. No statistical differences were found for VI and VA. These results suggested that the treatment of honeybee venom injection collected using bee venom collector could be used effectively for the increase productivity.

• 한방안이비인후피부과학회지
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• 제24권3호
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• pp.138-146
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• 2011

Objective : This study was performed to prove the effect of the oriental medical treatments on urticaria and angioedema caused from serum sickness reaction by bee venom. Method : We treated one case of urticaria and angioedema with oriental medical treatments such as 8 constitution acupuncture, herbal medicine, venesection therapy and facial pack therapy. We took pictures through the treatment to evaluate the improvement. Six items, itching, burning sensation, pain, wheal, redness, edema were used to diagnose urticaria and angioedema. Result & Conclusion : After treatments the urticaria and angioedema were improved. The symptom of itching, heating, pain, wheal, redness of whole body and facial edema disappeared.

• Journal of Acupuncture Research
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• 제33권4호
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• pp.181-190
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• 2016

Objectives : This study reports the clinical effects of Korean medical treatment on a patient with suspected diabetic muscle infarction (DMI). Methods : A patient diagnosed with spinal stenosis was suspected of DMI. The patient was treated with acupuncture, bee-venom pharmacopuncture and gastrocnemius stretching. Symptoms were evaluated by verbal numeric rating scale (vNRS), Oswestry disability index (ODI) and Manchester foot pain and disability index (MFPDI). Results : After approximately 5 weeks of Korean medical treatment, including 8 bee-venom treatments, vNRS, ODI, and MFPDI all decreased. Conclusion : The results suggest that Korean medical treatment is effective for treating pain caused by DMI.

• 혜화의학회지
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• 제17권1호
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• pp.145-155
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• 2008

Objective: There are some case reports on treatment of shoulder dislocation in Patients Undergoing Stroke. We report the electronic needle and the Bee venom Acupunure Theraphy about under using shoulder dislocation in patients Undergoing Stroke got a good remedial value. Methods : The patients diagnosed as shoulder dislocation and treated mainly with Bee venom Acupunture Therapy and electronic needle Results & Conclusions : Symptoms of the patientes such as shoulder pain, Range Of Motion(ROM) were improved after above treatments. The interval in X-ray film comes to be narrow. So, it is suggested that oriental medical treatment are effective on post stroke with shoulder dislocation.

• Journal of Acupuncture Research
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• 제23권2호
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• pp.21-31
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• 2006

In the present study, We have investigated the bee venom (BV) and melittin (a major component of BV)-mediated anti-proliferative effects, and defined its mechanisms of action in cultured rat aortic vascular smooth muscle cells (VSMCs). BV and melittin $(0.4{\sim}0.8\;{\mu}g/ml)$ effectively inhibited 5% FBS-induced VSMCs proliferations. The regulation of apoptosis has attracted much attention as a possible means of eliminating excessively proliferating VSMCs. In the present study, the treatment of BV and melittin strongly induced apoptosis of VSMCs. These results suggest that the anti-proliferative effects of BV and melittin in VSMCs should be related with induction of apoptosis. Further study about Influence of BV and melittin upon apoptosis mechanism is therefor thought to be necessary to confirm the above results.