• Korean Journal of Veterinary Service
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• v.38 no.1
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• pp.25-30
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• 2015

Bee venom has been used as to prevent and treat bovine mastitis as natural antimicrobial compounds in some dairy cattle farms in Korea. It is needed to determine the residual of bee venom in milks of dairy cattle treated with bee venom. Since bee venom is not approved as a raw material for animal drugs, the preprocessing method to detect bee venom residual in milk and the tolerance limit for its residue has not been established yet in Korea. Therefore, the purpose of this study was to develop pre-processing method not affecting major component of bee venom for detection of its residue in milks using ultra-high performance liauid chromatography (UHPLC). In addition, bee venom residue was also analyzed in milk samples of dairy cattle treated for mastitis with bee venom using UHPLC with the developed pre-processing method in this study. As a result, melittin, histamin and phospolipase A2, the major components of bee venom, were all detected by UHPLC with the pre-processing method developed in this study. The results of this study suggest that the pre-processing method developed in this study can be useful to detect bee venom residue in dairy cattle milk. We also found that no bee venom residues were detected in milk samples collected from dairy cattle treated with bee venom after 1 and 3 days, respectively.

• Journal of Pharmacopuncture
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• v.10 no.3
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• pp.77-83
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• 2007

Objectives The aim of this study is to investigate the effect of Sweet Bee Venom herbal acupuncture for patients with acute whiplash injury by Traffic Accident. Methods This clinical study was carried out 25 cases of acute whiplash injury patients which had been treatment in Woosuk oriental hospital from March, 2007 to September, 2007. Sweet bee venom herbal acupuncture(N=15) and normal saline(N=10) injected on the acupoints that were cervical area. I checked the VAS for the pain and ROM(range of motion) of the cervical. these were checked 3 times. one was before treatments, another was after 3 times treatments with sweet bee venom herbal acupuncture and normal saline injection, and the other was after 5 times treatments with sweet bee venom herbal acupuncture and normal saline injection. Results VAS score was significantly improved after 5 times treatments with the sweet bee venom herbal acupuncture compared to normal saline I.M. on the acupoints that was cervical area. There were significant changes in the sweet bee venom herbal acupuncture group with VAS and ROM check. Conclusions This study suggests that sweet bee venom herbal acupuncture can improve symptoms in patients with acute whiplash injury by traffic accident.

• Journal of Physiology & Pathology in Korean Medicine
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• v.19 no.1
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• pp.167-173
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• 2005

To investigate the possible molecular mechanism (s) of bee venom as a candidate of anti-cancer drug, we examined the effects of the compound on the growth of human lung carcinoma cell line A549. Bee venom treatment declined the cell growth and viability of A549 cells in a concentration-dependent manner, which was associated with induction of apoptotic cell death. Bee venom down-regulated the levels of anti-apoptotic genes such as Bcl-2 and Bcl-XS/L, however, the levels of Bax, a pro-apoptotic gene, were up-regulated. Bee venom treatment induced not only tumor suppressor p53 but also cyclin-dependent kinase inhibitor p21WAF1/CIP1 expression in a dose-dependent manner. Furthermore, bee venom treatment induced the down-regulation of telomerase reverse transcriptase mRNA and telomeric repeat binding factor expression of A549 cells, however, the levels of telomerase-associated protein-1 and c-myc were not affected. Taken together, these findings suggest that bee venom-induced inhibition of human lung cancer cell growth is associated with the induction of apoptotic cell death via regulation of several major growth regulatory gene products, and bee venom may have therapeutic potential in human lung cancer.

• IEMEK Journal of Embedded Systems and Applications
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• v.2 no.2
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• pp.128-136
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• 2007

무선 기술이 가지는 장점인 설치의 편리성, 기동성으로 인하여, ZigBee를 이용한 홈 네트워크 서비스 활용성에 대한 기술개발이 활발히 진행되고 있다. 이러한 유비쿼터스 홈 서비스의 활용성에도 불구하고 ZigBee 기반 홈 기기나 센서 정보의 도청, 비정상적인 패킷의 유통, 메시지 재사용등의 데이터 위/변조 문제와 네트워크 전체를 마비시킬 수 있는 서비스 거부 공격등에 쉽게 노출되어 있다. 본 논문에서는 이에 대한 효과적인 대응과 관리 처리를 위하여, 유비쿼터스 홈 네트워크에서의 ZigBee 기술을 사용할 시에 발생할 수 있는 보안 문제점을 해결하기 위한 ZigBee 기반 End-to-End 보안 기술을 개발하였다. 본 기술 개발의 특징은 ZigBee Device, 코디네이터/게이트웨이 영역은 ZigBee 표준 스펙을 구현하였으며, 응용어플리케이션/서버 영역은 표준 스펙이 정의 되어 있지 않으나, 이 부분을 유비쿼터스 홈 네트워크 서비스 관점에서 설계 및 구현하였다. ZigBee 디바이스가 가지는 하드웨어 자원의 빈약성을 고려하여 최대한 보안 기능을 리소스가 풍부한 응용어플리케이션/서버의 자원 활용에 촛점을 맞췄다.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.34 no.7B
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• pp.703-709
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• 2009

Recently, we are using wireless system based on ZigBee technology. It solves a complicated space and frequency movement. Then, we had studied it for improved performance. So, we are must concerned about routing protocol for improvement of a weak point of physical feature. But many researchers are not focusiong on developing Routing Protocol. In this paper, we proposed improved routing protocol using AODV for wireless system based on ZigBee technology. And it is analyzed the simulation result which compare with original and improved AODV protocol based on ZigBee network.

• Journal of Information Processing Systems
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• v.5 no.4
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• pp.221-228
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• 2009

Due to low cost, low-power, and scalability, ZigBee is considered an efficient wireless AMR infrastructure. However, these characteristics of ZigBee can make the devices more vulnerable to unexpected error environments. In this paper, a fault-tolerant wireless AMR network (FWAMR) is proposed, which is designed to improve the robustness of the conventional ZigBee-based AMR systems by coping well with dynamic error environments. The experimental results demonstrate that the FWAMR is considerably fault-tolerant compared with the conventional ZigBee-based AMR network.

• The Korean Journal of Pain
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• v.5 no.1
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• pp.57-62
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• 1992

Bee Venom therapy, as bee honey has long contributed to human life as a valuable fancy food since human history began. It can be found in the literature back up to a thousand years as a form of therapy for management of apin of various origin. Even to the present, there are some physicians and medical personnel who believe in the effects of bee sting therapy throughout the world. However Bee Venom therapy remains somewhat controversial and there are very few controlled studies available to guide clinical practice. We administered apitherapy(Bee venom therapy) against pain of musculoskeletal origin in thirty one patients from october 1988 to october 1990 and got successful results in seventy one percent of patients. Our results suggest that Bee Venom therapy can be one of the methods for conquering pain caused by true inflamation.

• Proceedings of the Korean Society of Computer Information Conference
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• 2016.01a
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• pp.69-70
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• 2016

ZigBee는 전력소모가 적고 근거리통신방식을 사용하며 저비용인 장점을 가지는 개인 영역 네트워크 (PAN)이다. ZigBee가 산업과 헬스케어 등의 여러 애플리케이션에서 많이 사용되면서, 최근 ZigBee에서의 보안이 중요한 문제로 다루어졌다. 본 논문에서는 ZigBee 네트워크에서의 키 관리 기법을 조사하고 보안능력 및 성능을 분석한다.

• Journal of Pharmacopuncture
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• v.3 no.2
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• pp.153-168
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• 2000

This study was designed to study on major components of various Bee Venom(Bee Venom by electrical stimulation in Korea; K-BV I, Bee Venom by Microwave stimulation in Korea; K -BV II, 0.5rng/ml, Fu Yu Pharmaceutical Factory, China; C-BV, 1mg /ml, Monmouth Pain Institute, Inc., U.S.A.; A-BV) using Electrophoresis. The results were summarized as follows: 1. In 1:4000 Bee Venom solution rate, the band was not displayed distinctly usmg Electrophoresis. But in 1: 1000, the band showed clearly. 2. The results of Electrophoresis at solution rate 1:1000, K-BV I and K-BVII showed similar band. 3. The molecular weight of Phospholipase $A_2$ was known as 19,000 but its band was seen at 17,000 in Electrophoresis. 4. Protein concentration of Bee Venom by Lowry method was different at solution rate 1:4000 ; C-BV was $250{wmu}g/ml,\;K-BV\;I\;was\;190{wmu}g/ml,\;K-BVII\;was\;160{wmu}/ml\;and\;C-BV\;was\;45{wmu}/ml5$. Electrophoresis method was unuseful for analysis of Bee Venom when solution rate is above 1:4000 but Protein concentration of Bee Venom by Lowry method was possible. These data from the study can be applied to establish the standard measurement of Bee Venom and prevent pure bee venom from mixing of another components. I think it is desirable to study more about safety of Bee Venom as time goes by.

• Journal of Physiology & Pathology in Korean Medicine
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• v.25 no.1
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• pp.132-137
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• 2011

Bee venom acupuncture (BVA), as a kind of herbal acupuncture, involved injecting diluted bee venom into acupoints and is used for pain, osteoarthritis and rheumatoid arthritis patients. BVA is growing in popularity, especially in Korea, and is used primarily for pain relief in many kinds of diseases. However, the effect of bee venom anti-inflammatory related action in lipopolysaccharide (LPS) induced chondrocyte stress have not been reported yet. The aim of this study was to investigate the effect of bee venom of cell viability and inflammatory cytokine in rat articular chondrocyte cultures stimulated with lipopolysaccharide. Inflammation was induced in rat chondrocytes by treatment with $10{\mu}g/m{\ell}$ LPS. The change of cell viability were decreased in chondrocytes after treatment with lipopolysaccharide. The cell viability revealed that BV exerted no significant cytotoxicity in the rat chondrocyte. Bee venom inhibited decreased cell viability in the presence of lipopolysaccharide ($10{\mu}g/m{\ell}$) in a dose dependent manner(0.1, 0.5, 1.0 and $5.0{\mu}g/m{\ell}$) at bee venom(p<0.05). Tumor necrosis factor (TNF)-${\alpha}$ production in the presence of lipopolysaccharide($1{\mu}g/m{\ell}$) was also inhibited in a dose dependent manner (p<0.05 from bee venom $0.1{\mu}g/m{\ell}$). Interleukin (IL)-6 production in the presence of lipopolysaccharide ($10{\mu}g/m{\ell}$) was inhibited as well (p<0.05 at bee venom 0.1, 0.5, 1.0 and $5.0{\mu}g/m{\ell}$, respectively). Our results demonstrate that bee venom was a anti-inflammatory agent of chondrocytes. Bee venom may exert its anti inflammatory effects through inhibition of TNF-${\alpha}$ and IL-6 synthesis, and may then pain relief and reduce the articular destruction.