• 제목/요약/키워드: batch

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Maximization of cell growth and polysaccharide production from Agaricus blazei by fed-batch cultivation

  • 황정민;서정식;권명상;최정우;한진수;홍억기
    • 한국생물공학회:학술대회논문집
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    • 한국생물공학회 2000년도 추계학술발표대회 및 bio-venture fair
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    • pp.283-286
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    • 2000
  • 본 연구에서는 fed-batch를 통하여 Agaricus blazei의 균체증식과 다당체 생산을 증가시키고자 공급배지와 배지공급속도에 변화를 주어 균체증식과 다당체 생성에 미치는 영향을 비교 ${\cdot}$ 검토하였다. 유가식 배양에서의 최종균체량 및 다당체 생성량은 회분식 배양에 비해 훨씬 높았으며, 초기에 균체증식을 위해 공급배지로 yeast extract를 먼저 공급하고 4일째부터 다당체 생성을 위해서 glucose를 공급할 경우 균체량은 18.2 g/L, 다당체 생성량은 10.4 g/L를 생산하여 배양결과 최대 생산량을 나타내었다.

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Xylitol의 생산성 향상을 위한 Two-stage Fed-batch 배양조건의 최적화

  • 조영일;서진호;유연우
    • 한국생물공학회:학술대회논문집
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    • 한국생물공학회 2000년도 추계학술발표대회 및 bio-venture fair
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    • pp.356-359
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    • 2000
  • Xylitol의 생산성을 높이기 위해 two-stage fed-batch를 수행하였다. Glucose가 고갈되어 pH가 5.7에서 올라가면 glucose를 공급하는 방법에서 최종세포의 OD 185.0과 최종 ethanol 농도 1.0 g/L를 얻었다. 산소전달에 대한 xylitol의 생성 영향에서는 통기량 1 vvm에서 500 rpm의 교반속도일 때 xylitol 수율 55.2%와 생산성 $2.19\;g-xylitol/L\;{\cdot}\;h$를 얻었다.

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Enhanced production in recycle fed-batch cultivation by Lactic acid bacteria Isolated from Kimchi

  • Joe, Lim;Kwun, Kyu-Hyuk;Chang, Hae-Choon;Lee, Jung-Heon
    • 한국생물공학회:학술대회논문집
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    • 한국생물공학회 2005년도 생물공학의 동향(XVII)
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    • pp.312-315
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    • 2005
  • A process for efficient recycle fed-batch culture was carried out to increase cell mass and spore production by Lactic acid bacteria isolated from Kimchi. A large quantity of cell mass obtained by feeding concentration of sugar in recycle fed-batch culture. When the high density of salt was created that the cell mass was come-down. In this study, cultured in different feeding concentration of sugar conditions. Lactic acid bacteria by recycle fed-batch culture was investigated in 2L working volume of fermenter, obtained the maximum cell mass was 15.17g/L.

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Order Batch Formations for Less Picker Blocking in a Narrow-Aisle Picking System

  • Hong, Soondo
    • Industrial Engineering and Management Systems
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    • 제14권3호
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    • pp.289-298
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    • 2015
  • This paper analyses the best batch formations for order picking throughput in narrow-aisle order picking systems. Our analytical comparison finds that a high pick density variation leads to a heavy picker blocking. Simulation experiments show that a distance-based batching algorithm reduces picker blocking by decreasing the number of aisles visited and stabilizing the variation in number of picks per aisle by packing orders tightly, and that the solution quality and mechanism for determining the batch size dictated by the sorting strategy causes varying amounts of blocking. We conclude that combining a distance-based batching method with an appropriate batch sizing strategy will reduce picker blocking and shorten travel in narrow-aisle picking systems.

주문 집약 문제에 대한 휴리스틱 기법 (The Heuristic Approach to the Order Consolidation Problem)

  • 박종호;임경국;최봉하
    • 대한산업공학회지
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    • 제34권4호
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    • pp.408-413
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    • 2008
  • We consider the batch processing of orders where either whole or part of a single order or a specific pair of different orders may be grouped in a batch within a fixed capacity. Our objective is to maximize the total number of batches filled up to the batch size. In this paper, we study the Level-2 problem where at most 2 kinds of orders can be grouped in a batch. This problem is known to be NP-hard and Max SNP-hard. So we develop heuristic algorithm and evaluate the performance of the algorithm.

NONLINEAR IMPULSIVE SYSTEM OF MICROBIAL PRODUCTION IN FED-BATCH CULTURE AND ITS OPTIMAL CONTROL

  • GAO CAIXIA;LANG YANHUAI;FENG ENMIN;XIU ZHILONG
    • Journal of applied mathematics & informatics
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    • 제19권1_2호
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    • pp.203-214
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    • 2005
  • In this study the optimal control of fed-batch glycerol fermentation is investigated based on an impulsive dynamical system. Considering the sudden increase of the glycerol and alkali in fed-batch culture of biodissimilation of glycerol to 1,3-propanediol, this paper proposes a non-linear impulsive system of fed-batch culture. The existence, uniqueness and regularity properties of piecewise solution for the system are proved. In view of the controllability of volumes of glycerol added to the reactor instantaneously, the paper constructs an optimal control model based on the nonlinear impulsive system and the existence of the optimal control is obtained. The control variables here are the moments and the sizes of jumps in the states at the discrete instants and the objective is to maximize the productivity of 1,3-propanediol over one cycle.

Optimization of Producing Liquid Fuel from Photosynthetic Algal Growth

  • Pak, Jin-Hong;Lee, Shin-Young
    • Journal of Microbiology and Biotechnology
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    • 제1권2호
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    • pp.111-115
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    • 1991
  • The green alga, Dunaliella salina under fed-batch cultivation produced 51.12 mg of hydrocarbon per liter with maintaining 0.313 (g dry wt/l). About 20% of hydrocarbon production yield based on dry biomass was obtained from both batch and fed-batch processes. Optimum culture conditions of light intensity, pH and salt concentration were obtained as 0.0080 (kJ/$cm^2$/h), 8.0 and 1.4 (g of NaCl/l), respectively by response surface analysis. The production of hydrocarbons in D. salina was closely correlated to cell growth. Fed-batch cultivation produced more hydrocarbons and maintained better cell growth than a batch process.

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Ethanol Production from Lignocellulosic Biomass by Simultaneous Saccharification and Fermentation Employing the Reuse of Yeast and Enzyme

  • KIM, JUN-SUK;KYUNG-KEUN OH;SEUNG-WOOK KIM;YONG-SEOB JEONG;SUK-IN HONG
    • Journal of Microbiology and Biotechnology
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    • 제9권3호
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    • pp.297-302
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    • 1999
  • Simultaneous saccharification and fermentation (SSF) experiments were carried out with a lignocellulosic biomass. The effects of temperature on enzymatic saccharification and the ethanol fermentation were also investigated. The batch SSF process gave a final ethanol concentration of 10.44 g/l and equivalent glucose yield of 0.55 g/g, which was increased by 67% or higher over the saccharification at 42℃. The optimal operating condition was found to vary in several parameters, such as the transmembrane pressure, permeation rate, and separation coefficient, related to the SSF combined with membrane system (semi-batch system). When the fermentation was operated in a semi-batch mode, the efficiency of the enzymes and yeast lasted three times longer than in a batch mode.

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420J2마르텐사이트 스테인레스강의 최종경도에 미치는 열처리조건의 영향 (Effect of Heat Treatments on the Final Hardness of STS 420J2 Martensitic Stainless Steel)

  • 김기돈;성장현
    • 열처리공학회지
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    • 제7권3호
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    • pp.175-183
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    • 1994
  • The effect of batch annealing conditions and austenitizing temperatures on the hardness and microstructural factors were examined by using 420J2 martensitic stainless steel. In spite of the similler hardness after batch annealing, the difference in hardness at the same austenitizing temperature was caused by changes in dissolved carbon during batch annealing. The highest hardness of the specimen was obtained at the batch annealing temperature of $820^{\circ}C$ and austenitizing temperature of $1050^{\circ}C$. The main factor affecting the final hardness of the cold annealed 420J2 specimen was proved to the austenitizing temperature rather than batch annealing temperature.

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누적 일괄추정 기법을 이용한 수동 선배열 소나 방위 주파수 - 표적기동분석 연구 (Study on Bearing and Frequency Target Motion Analysis for Passive Line Array SONAR Using Accumulative Batch Estimation)

  • 김인수
    • 제어로봇시스템학회논문지
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    • 제22권10호
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    • pp.788-796
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    • 2016
  • Bearing and frequency measurements of TMA (Target Motion Analysis) in passive line array SONAR have lower bearing rate and frequency doppler, and are not detected or tracked continuously because of various ocean environments. This is a main reason to effect the TMA performance and it takes a long time to get TMA solutions. We propose the bearing and frequency TMA(BFTMA) using accumulative batch estimation to solve the TMA problem of line array passive SONAR. The accumulative batch estimation structure is based on MLE (Maximum Likelihood Estimation) but used accumulative measurements. The accumulative batch estimation is applied for the BFTMA with nonlinear Kalman filter to estimate the target range, speed and course. Simulation and sea data analysis were carried out to verify the performance and applicability of the proposed techniques.