• Biotechnology and Bioprocess Engineering:BBE
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• v.7 no.4
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• pp.234-236
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• 2002

A process for the production of mannitol from fructose (5% to 25%) using Leuconostoc mesenteroides NRRL B-1149 was investigated. Fermentations were carried out In batch or fed-batch fermentations without aeration at 28$\^{C}$, pH 5.0. When 5% fructose was used In batch culture fermentation, the yield of mannitol was 78% of that expected theoretically. When the frurtose concentration was Increased to 10%, the yield dropped to 59.6% of the theoretical value. However, In the fed-batch culture, using 10% fructose, the yield was 81.9% of the theoretical value. In a 15% fruttose fed-batch culture, with 5% fructose being added initially and the other 10% fructose being added as a continuous supply the final yield was 83.7% of the theoretical yield. When 20% fructose was used In the same manner, the yield was 89.5% of theoretical yield.

• Environmental Engineering Research
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• v.11 no.2
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• pp.63-76
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• 2006

Multivariate analysis and batch monitoring on a pilot-scale sequencing batch reactor (SBR) are described for integrated wastewater treatment management system, where a batchwise multiway independent component analysis method (MICA) are used to extract meaningful hidden information from non-Gaussian wastewater treatment data. Three-way batch data of SBR are unfolded batch-wisely, and then a non-Gaussian multivariate monitoring method is used to capture the non-Gaussian characteristics of normal batches in biological wastewater treatment plant. It is successfully applied to an 80L SBR for biological wastewater treatment, which is characterized by a variety of error sources with non-Gaussian characteristics. The batchwise multivariate monitoring results of a pilot-scale SBR for integrated wastewater treatment management system showed more powerful monitoring performance on a WWTP application than the conventional method since it can extract non-Gaussian source signals which are independent and cross-correlation of variables.

• Environmental Engineering Research
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• v.20 no.1
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• pp.65-72
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• 2015

A comparative study on response of a toxic compound thiocyanate ($SCN^-$) was carried out in continuous and fed batch moving bed reactor systems. Both systems had three sequential anaerobic, anoxic and aerobic reactors and operated at same hydraulic retention time. Feed $SCN^-$ was first increased from 600 mg/L to 1,000 mg/L for 3 days (shock 1) and then from 600 to 1,200 mg/L for 3 days (shock 2). In anaerobic continuous reactor, increase of effluent COD (chemical oxygen demand) due to shock load was only 2%, whereas in fed batch reactor it was 14%. In anoxic fed batch reactor recovery was partial in terms of $SCN^-$, phenol, COD and $NO{_3}{^-}$-N and $NO{_2}{^-}$-N removals and in continuous reactor complete recovery was possible. In both systems, inhibition was more significant on aerobic reactors than anaerobic and anoxic reactors. In aerobic reactors ammonia removal efficiency deteriorated and damage was irreversible. Present study showed that fed batch reactors showed higher substrate removal efficiency than continuous reactors during regular operation, but are more susceptible to toxic feed shock load and in nitrifying reactor damage was irreversible.

• Microbiology and Biotechnology Letters
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• v.20 no.2
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• pp.196-202
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• 1992

Zoogloea ramigera 115 was selected for the production of viscous microbial polysaccharide for bioflocculants usage. Batch, fed-batch, and continuous culture processes were examined with regard to the high biopolymer production. Several carbon sources were tested, including glucose, lactose, molasses, and cheese whey. The C/N ratio of 90 was most effective for biopolymer production from glucose, while the C/N ratios of 30 for lactose and 60 for both molasses and cheese whey substrate gave a maximum production. Fed batch culture proved more effective to increase final biopolymer concentration than batch culture. Continuous fermentation with two stages modifying C/N ratio increased the productivity. The production rates were a maximum at dilution rate of 0.048 $hr^{-1}$ for molasses and at 0.096 $hr^{-1}$for cheese whey.

• Proceedings of the Korean Radioactive Waste Society Conference
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• 2003.11a
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• pp.358-362
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• 2003

An electrochemical reduction technology which can reduce the decay heat, volume, and radioactivity of spent fuel by a factor of quarter by converting oxide type spent fuel to a metallic form in a molten salt was developed and mock-up test in a 5kg $U_3O_8$/batch scale was carried out. The electrochemical reaction was analyzed regarding the operational factors. The research efforts was also concentrated on the apparatus development for a hot test. Fresh $U_3O_8$ powder was metallized with a more than 99% yield via this electrochemical technology and design data for the 20kg $U_3O_8$/batch scale apparatus were also obtained.

• Journal of Korean Society of Water and Wastewater
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• v.18 no.2
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• pp.113-120
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• 2004

The biodegradability of organics has become essential for the design and modeling of a biological nutrient removal process. Respirometry for the batch test just with wastewater has been conducted to estimate active biomass and readily biodegradable organics in municipal wastewater simultaneously. Municipal wastewater contains significant active biomass, which is estimated about 17% of COD. Compared to the batch test seeded with sludge, the batch test just with wastewater represents a little higher readily biodegradable organics. This might be due to the different environment of the logarithmic growth of active biomass. The nitrate uptake rate test has been also performed for the estimation of the readily biodegradable organics. The nitrate uptake rate test results in a little higher readily biodegradable organics compared to the batch test seeded with sludge and similar organics compared to the batch test just with wastewater. This might be caused by the different sludge of a sequencing batch reactor process. Taking the result of the previous research into account, the readily biodegradable, slowly biodegradable, active biomass, soluble inert, and particulate inert organics are estimated about 11%, 49%, 17%, 11%, and 12% of COD, respectively.

• Journal of Microbiology and Biotechnology
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• v.22 no.6
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• pp.826-831
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• 2012

Acarbose, a pseudo-oligosaccharide, is widely used clinically in therapies for non-insulin-dependent diabetes. In the present study, S-adenosylmethionine (SAM) was added to selected media in order to investigate its effect on acarbose fermentation by Actinoplanes utahensis ZJB-08196. Acarbose titer was seen to increase markedly when concentrations of SAM were added over a period of time. The effects of glucose and maltose on the production of acarbose were investigated in both batch and fed-batch fermentation. Optimal acarbose production was observed at relatively low glucose levels and high maltose levels. Based on these results, a further fed-batch experiment was designed so as to enhance the production of acarbose. Fed-batch fermentation was carried out at an initial glucose level of 10 g/l and an initial maltose level of 60 g/l. Then, 12 h post inoculation, 100 ${\mu}mol/l$ SAM was added. In addition, 8 g/l of glucose was added every 24 h, and 20 g/l of maltose was added at 96 h. By way of this novel feeding strategy, the maximum titer of acarbose achieved was 6,113 mg/l at 192 h. To our knowledge, the production level of acarbose achieved in this study is the highest ever reported.

• Journal of Environmental Health Sciences
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• v.31 no.2 s.83
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• pp.152-159
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• 2005

Laboratory scale experiments were conducted to study the applicability, and to compare the performance of two types of sequencing batch reactor (SBR)systems, a conventional SBR and sequencing batch biofilm reactor (SBBR) on the biological nitrogen and phosphorus removal. The nitrification rate in SaR was higher than that in SBBR both in high influent TOC concentration. The denitrification was completed at the first non-aeration period in SBR, however, the additional non-aeration period should be installed or the first aeration period should be extended more in order to complete the nitrogen removal in SBBR. The time at the first aeration period was more needed as about 4-5 h in order to uptake all the released $PO_4^{3-}\;-P$ at the first non-aeration period. SBBR needed more operation time, especially the first aeration time, than SBR at the high influent TOC concentration in order to complete nitrogen and phosphorus removal.

• Journal of Microbiology and Biotechnology
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• v.7 no.6
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• pp.417-422
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• 1997

Recombinant Bacillus subtilis LKS88[pASA240] containing the amylase gene from Streptomyces albus KSM-35 was exploited in fed-batch cultivation for mass production of maltotetraose-producing amylase. The effects of dissolved oxygen, additional organic nutrients (peptone and yeast extract) and mixed carbon sources (glucose plus soluble starch) on amylase production were examined in fed-batch operations in an effort to determine the optimum conditions for a maximum amylase productivity. Under the optimum conditions, maximum amylase activity was about 4.2 times higher than that obtained in batch cultivations, indicating that mass production of maltotetraose-producing amylase could be accomplished in fed-batch cultivation of the recombinant B. subtilis strain.

• Journal of Broadcast Engineering
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• v.13 no.1
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• pp.69-78
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• 2008

Batch signature is a cryptographic tool which efficiently generates several signatures for multiple messages. Previously proposed batch signature schemes do not allow a signer to generate a signature immediately for sequentially asked signing queries. In other words, the previous schemes are not applicable when signing requests are not simultaneously given to the signer. In this paper, we propose flexible batch signatures which can provide batch signing for sequential signing requests. Moreover, our schemes are well suited for imbalanced environment where two communicating parties have different computing powers.