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http://dx.doi.org/10.4014/jmb.1111.11047

Significantly Enhanced Production of Acarbose in Fed-Batch Fermentation with the Addition of S-Adenosylmethionine  

Sun, Li-Hui (Institute of Bioengineering, Zhejiang University of Technology)
Li, Ming-Gang (Institute of Bioengineering, Zhejiang University of Technology)
Wang, Yuan-Shan (Institute of Bioengineering, Zhejiang University of Technology)
Zheng, Yu-Guo (Institute of Bioengineering, Zhejiang University of Technology)
Publication Information
Journal of Microbiology and Biotechnology / v.22, no.6, 2012 , pp. 826-831 More about this Journal
Abstract
Acarbose, a pseudo-oligosaccharide, is widely used clinically in therapies for non-insulin-dependent diabetes. In the present study, S-adenosylmethionine (SAM) was added to selected media in order to investigate its effect on acarbose fermentation by Actinoplanes utahensis ZJB-08196. Acarbose titer was seen to increase markedly when concentrations of SAM were added over a period of time. The effects of glucose and maltose on the production of acarbose were investigated in both batch and fed-batch fermentation. Optimal acarbose production was observed at relatively low glucose levels and high maltose levels. Based on these results, a further fed-batch experiment was designed so as to enhance the production of acarbose. Fed-batch fermentation was carried out at an initial glucose level of 10 g/l and an initial maltose level of 60 g/l. Then, 12 h post inoculation, 100 ${\mu}mol/l$ SAM was added. In addition, 8 g/l of glucose was added every 24 h, and 20 g/l of maltose was added at 96 h. By way of this novel feeding strategy, the maximum titer of acarbose achieved was 6,113 mg/l at 192 h. To our knowledge, the production level of acarbose achieved in this study is the highest ever reported.
Keywords
Acarbose; S-adenosylmethionine; fed-batch; Actinoplanes utahensis;
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