• 한국작물학회지
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• 제47권2호
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• pp.85-94
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• 2002

Antibodies raised against the purified p-subunit of $\beta$-conglycinin were used in immunohistochemical studies to monitor the pattern of $\beta$-conglycinin mobilization in the cotyledons during soybean [Glycine max (L.) Merr.] seed germination. Western blot analysis revealed that the break down of the $\beta$-subunit of $\beta$-conglycinin commenced as early as 2 days after seed imbibition (DAI). Concurrent with the degradation of the $\beta$-subunit of $\beta$-conglycinin, accumulation of 48, 28, and 26 kD proteolytic intermediates was observed from 2 to 6 DAI. Western blot analysis also revealed that the acidic subunit of glycinin was mobilized earlier than the basic subunit. The basic glycinin subunit was subjected to proteolysis within 2 DAI resulting in the appearance of an intermediate product approximately 2 kD smaller than the native basic glycinin subunit. In contrast to the major seed storage proteins, lipoxygenase was subjected to limited proteolysis and was detected even after 8 DAI. The first sign of $\beta$-conglycinin breakdown was observed near the vascular strands and proceeded from the vascular strands towards the epidermis. Protein A-gold localization studies using thin sections of soybean cotyledons and antibodies raised against the $\beta$-subunit of $\beta$-conglycinin revealed intense labeling over protein bodies. A pronounced decrease in the protein A-gold labeling intensity over protein bodies was observed at later stages of seed germination. The protein bodies, which were converted into a large central vacuole by 8 DAI, contained very little 7S protein as evidenced by sparse protein A-gold labeling in the vacuoles.

• Preventive Nutrition and Food Science
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• 제2권1호
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• pp.77-82
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• 1997

The comparison soy protein coagulation by heat-and enzyme-treatment are summarized. The gelation mechanism of glycinin by heating was mainly due to dissociation and aggregation of the basic subunit of 11S globulin. In case of 7S globulin, macro-soluble aggregates may be formed by noncovalent intraction more than 30min at 8$0^{\circ}C$. Whereas, coagulum occured by the microbial enzyme was more minuter than the other Ca-, HCI-coagulum. Heat treatment attacked the basic subunit of 11S globulin and this results agreed very, how-ever, preferred acidic subunit to basic subunit of 11S globulin and attacked the 7S globulin, that could produce coagulum products within 4~5min at $65^{\circ}C$.

• 한국식품영양과학회지
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• 제22권3호
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• pp.273-279
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• 1993

박테리아 Bacillus sp. K-295G-7이 생산하는 두유응고효소 I 및 II에 의한 11S globulin (glycinin)의 가수분해 패턴을 조사하였다. 효소 I 과 II에 의한 acidic subunit의 응고시간은 약 4-5 분이었다. 전기 영동의 결과, acidic subunit (A$_3$, M.W=45,000)는 효소반응 2분 이내에 완전히 가수분해되어 분자량 16,000, 20,000의 새로운 band를 형성하였다. 한편 효소 II의 작용으로 약 30,000의 분자량을 가진 분해산물을 생성하였고 효소 I 과 II의 basic subunit 에 대한 가수분해 패턴은 유사하였다.

• Journal of Korean Biological Nursing Science
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• 제1권1호
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• pp.1-24
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• 1999

The purpose of this study was to define the content of requisite human structure and function knowledge needed for clinical knowledge of nursing practice. Subjects of human structure and function were divided into 10 units, and each unit was further divided into 21 subunits, resulting in a total of 90 items. Contents of knowledge of human structure and function were constructed from syllabus of basic nursing subjects in 4 college of nursing, and textbooks published by nurse scholars prepared with basic nursing sciences. The degree of need of 90 items was measured with a 4 point scale. The subjects of this study were college graduated 136 nurses from seven university hospitals in Seoul and three university hospitals located in Chonnam Province, Kyungbook Province, and Inchon. They have been working at internal medicine ward, surgical ward, intensive care unit, obstetrics and gynecology ward, pediatrics ward, opthalmology ward, ear, nose, and throat ward, emergency room, rehabilitation ward, cancer ward, hospice ward, and their working period was mostly under 5 years. The results were as follows: 1. The highest scored items of human structure and function knowledge necessary for nursing practice were electrolyte balance, blood clotting mechanism and anticoagulation mechanism, hematopoietic function, body fluid balance, function of plasma, and anatomical terminology in the order of importance. The lowest scored items of human structure and function knowledge necessary for nursing practice was sexual factors of genetic mutation. 2. The highest order of need according to unit was membrane transport in the living unit, anatomical terminology in movement and exercise unit, mechanism of hormone function in regulation and integration unit, component and function of blood in oxygenation function unit, structure and function of digestive system in digestive and energy metabolism unit, temperature regulation in temperature regulation unit electrolyte balance in body fluid and electrolyte unit, concept of immunity in body resistance unit, and genetics terminology in genetics unit. The highest order of importance according to subunit was membrane transportation in cell subunit, classification of tissues in tissue unit, function of skin and skin in skin subunit, anatomical derivatives of the skeleton subunit, classification of joints in joint subunit, an effect of exercise on muscles in muscle subunit, function of brain in nervous system subunit, special sense in sensory subunit mechanism of hormone function in endocrine subunit, structure and function of female reproductive system in reproductive system unit, structure and function of blood in blood unit, structure of heart, electrical and mechanical function in cardiovascular system unit, structure of respiratory system in respiratory system subunit, structure and function of digestive system in digestive system subunit, hormonal regulation of metabolism in nutrition and metabolism subunit, function of kidney in urologic system subunit, electolyte balance in body fluid, electolyte and acid-base balance subunit. 3. The common content of human structure and function knowledge need for all clinical areas in nursing was structure and function of blood, hematopoietic function, function of plasm, coagulation mechanism and anticoagulation mechanism, body fluid, electrolyte balance, and acid-base balance. However, the degree of need of each human structure and function knowledge was different depending on clinical areas. 4. Significant differences in human structure and function knowledge necessary for nursing practice such as skin and derivatives of the skin, growth and development of bone, classification of joint, classification of muscle, structure of muscle, function of muscle, function of spinal cord, peripheral nerve, structure and function of pancrease, component and function of blood, function of plasma, structure and function of blood, hemodynamics, respiratory dynamics, gas transport, regulation of respiration, chemical digestion of foods, absorption of foods, characteristics of nutrients, metabolism and hormonal regulation, body energy balance were demonstrated according to the duration of work. 5. Significant differences in human structure and function knowledge necessary for nursing practice such as classification of tissue, classification of muscles, function of muscles, muscle metabolism, classification of skeletal muscles, classification of nervous system, neurotransmitters, mechanism of hormone function, pituitary and pituitary hormone, structure and function of male reproductive organ, structure and function of female reproductive organ, component and function of blood, function of plasma, coagulation mechanism and anticoagulation mechanism, gas exchange, gas transport, regulation of respiration, characteristics of nutrients, energy balance, function of kidney, concept of immunity, classification and function of immunity were shown according to the work area. Based on these findings, all the 90 items constructed by Korean Academic Society of Basic Nursing Science should be included as contents of human structure and function knowledge.

• Mycobiology
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• 제41권4호
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• pp.245-247
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• 2013

The ambrosia beetle, Platypus koryoensis, is an economically important pest affecting oak trees in Korea. Candida kashinagacola was isolated from galleries of the beetle in oak wood and identified by analyses of morphology, physiological properties, and nucleotide sequence of the large subunit ribosomal DNA. This is the first report on Candida species associated with oak wilt disease vectored by the ambrosia beetle, Platypus koryoensis, in Korea.

• BMB Reports
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• 제50권8호
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• pp.411-416
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• 2017

The endoplasmic reticulum (ER) membrane protein complex subunit 6 (EMC6) is a novel human autophagy-related molecule. Here, using tissue microarray and immunohistochemistry, we report that EMC6 protein is lost or reduced in glandular cells of patients with gastric adenocarcinoma, compared to normal stomach mucosa. Overexpression of EMC6 in gastric cancer cells inhibited cell growth, migration, invasion, and induced apoptosis and cell cycle arrest at S-phase. Further investigation suggested that EMC6 overexpression in BGC823 human adenocarcinoma gastric cancer cells reduced tumorigenicity in a xenograft model, demonstrating that EMC6 has the characteristics of a tumor suppressor. This is the first study to show that EMC6 induces cell death in gastric cancer cells. The molecular mechanism of how EMC6 functions as a tumor suppressor needs to be further explored.

• Applied Biological Chemistry
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• 제31권4호
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• pp.361-370
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• 1988

유청 및 대두 단백질의 상호작용을 규명하기 위하여 두 단백질용액 및 1 : 1 혼합용액에 대한 겔투과크로마토그래피와 전기영동에 의하여 열처리 중 조성단백질의 변화 및 상호작용에 관여한 조성단백질을 조사하였다. 크로마토그래피 결과 $80^{\circ}C$ 이상에서 열처리할 경우, 대두단백질 및 혼합물에서 저분자량의 단백질과 고분자량의 응집물이 증가한 것으로 나타났는데, 이는 열처리에 의하여 대두의 11S globulin이 subunit로 해리되고, 이것이 thiol기, disulfide bond 등을 함유한 유청단백질과 가용성 응질물을 형성하기 때문으로 생각되었다. 전기영동에 의하여, 가열시 유청조성단백질 중의 ${\beta}-Lactoglobulin$, ${\alpha}-Lactalbumin$ 및 proteose-peptone 3이 대두단백질과 상호작용을 일으키는 것으로 나타났다. 그리고 용액의 염환경에 따라 Bovine Serum Albumin, Immunoglobulin G(H) 및 Lactoferrin도 상호작용을 일으킬 수 있으며, 대두조성단백질 중의 11S globulin의 basic subunit와 acidic subunit, 7S globulin의 ${\alpha}'$ subunit가 유청단백질과 상호작용을 일으킬 수 있음을 추측할 수 있었다.

• Journal of Microbiology
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• 제42권2호
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• pp.152-155
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• 2004

Pseudomonas sp. K82 has been reported to be an aniline-assimilating soil bacterium. However, this strain can use not only aniline as a sole carbon and energy source, but can also utilize benzoate, p-hydroxybenzoate, and aniline analogues. The strain accomplishes this metabolic diversity by using dif-ferent aerobic pathways. Pseudomonas sp. K82, when cultured in p-hydroxybenzoate, showed extradiol cleavage activity of protocatechuate. In accordance with those findings, our study attempted the puri-fication of protocatechuate 4,5-dioxygenase (PCD 4,5). However the purified PCD 4,5 was found to be very unstable during purification. After Q-sepharose chromatography was performed, the crude enzyme activity was augmented by a factor of approximately 4.7. From the Q-sepharose fraction which exhibited PCD 4,5 activity, two subunits of PCD4,5 (${\alpha}$ subunit and ${\beta}$ subunit) were identified using the N-terminal amino acid sequences of 15 amino acid residues. These subunits were found to have more than 90％ sequence homology with PmdA and PmdB of Comamonas testosteroni. The molecular weight of the native enzyme was estimated to be approximately 54 kDa, suggesting that PCD4,5 exists as a het-erodimer (${\alpha}$$_1$${\beta}$$_1$). PCD 4,5 exhibits stringent substrate specificity for protocatechuate and its optimal activity occurs at pH 9 and 15 $^{\circ}C$. PCR amplification of these two subunits of PCD4,5 revealed that the ${\alpha}$ subunit and ${\beta}$ subunit occurred in tandem. Our results suggest that Pseudomonas sp. K82 induced PCD 4,5 for the purpose of p-hydroxybenzoate degradation.

• 한국균학회지
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• 제48권1호
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• pp.55-61
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• 2020

꼬마초롱이끼의 헛뿌리와 가는털깃털이끼의 잎에서 내생균을 분리하였다. 분리된 균주는 형태적 특성과 internal transcribed spacer, large subunit rDNA, Beta-tublin 영역 및 RNA polymerase II second largest subunit gene의 분자적 분석을 토대로 동정하였다. 연구 과정에서 2종의 국내 미기록종 내생균인 Cercophora thailandica와 Biscogniauxia petrensis를 확인하였다. 분리된 미기록종 내생균 균주의 형태적 특성 확인 및 계통 분석 결과에 대해 기술하였다.

• 식물분류학회지
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• 제33권3호
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• pp.255-270
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• 2003

한국산 팥꽃나무과(Thymelaeaceae Adans.)의 1속(아마풀속: 1종)을 제외한 4속(팥꽃나무속: 4종, 삼지닥나무속 1종, 피뿌리풀속 1종, 산닥나무속 2종)의 화분을 광학현미경(LM)과 주사전자현미경(SEM)으로 관찰하였다. 화분의 크기는 $11.3-60.0{\mu}m$로 소립(피뿌리풀속)에서 중립(팥꽃나무속, 산닥나무속), 대립(삼지닥나무속)까지 다양하게 나타났다. 조사된 분류군의 화분은 모두 단립으로 구형이고, 발아구의 형태는 산공형(10-20개)이다. 외표벽의 표면 무늬는 다소 차이는 있지만 삼면각(trihedral)의 부속요소(subunit)들이 원으로 배열하여 망상의 전형적인 "croton pattern"을 가지고 있다. 모든 부속요소의 중앙에는 하나의 미세돌기가 있고 부속요소의 표면은 평활상(psilate)이다. 팥꽃나무속 화분은 부속요소의 배열에 따라 세가지 유형으로 구별되어진다; (1) Type I: 부속요소들이 서로 붙어서 원을 이루고 부분적으로 연결이 끊어져 있는 형태, (2) Type II: 하나의 독립된 부속요소가 세 개의 면이 뚜렷한 삼면각을 이루는 형태, (3) Type III: 각 부속요소는 독립적으로 존재하나 뚜렷하지 못한 형태. 또한 한국산 팥꽃나무과에 대한 화분형질의 분류학적 적용에 대하여 간단하게 검토하였다.