• Bulletin of the Korean Chemical Society
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• 제24권8호
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• pp.1163-1168
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• 2003

The structures of molecular species of galactolipids, such as monogalactosyl diacylglycerol (MGDG) and digalactosyl diacylglycerol (DGDG), isolated from wheat flour have been investigated using negative-ion electrospray ionization (ESI) mass spectrometry interfaced with high performance liquid chromatography (HPLC). According to the result of HPLC analysis, MGDG and DGDG were found to consist of mixtures of five and four molecular species, respectively. The galactolipids have been also analyzed to determine their fatty acid compositions, using HPLC/ESI-MS combined with in-source (or cone voltage) fragmentation. HPLC/ ESI-MS is very useful for one-step analysis of mixtures of galactolipids with a small sample quantity. Especially, the carboxylate anions produced in in-source fragmentations of the negative-ion of each component separated by HPLC provide valuable information on the composition of its fatty acyl chains.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2003년도 추계학술대회 발표논문 초록집
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• pp.176-176
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• 2003

• 한국철도학회:학술대회논문집
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• 한국철도학회 2007년도 추계학술대회 논문집
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• pp.629-632
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• 2007

Communications Based Train Control(CBTC) is a continuous, automatic train control system utilizing high-resolution, train location determination independent of track circuits; continuous, high capacity, bi-directional train-to-wayside data communications; and trainborne and wayside processors capable of implementing vital functions. In this paper, we present a basic design of CBTC system based on ERTMS/ETCS specifications. The basic design is composed of system requirements analysis, system functional analysis, system architectural design, sub-system requirements analysis, sub-system functional analysis. Each deliverable has been produced according to a development process based on UML/SysML.

• 한국안전학회지
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• 제12권1호
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• pp.113-121
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• 1997

Human reliability attempts to make precise quantitative analyses and predictions of the performance of human-machine(or product) systems. In order to yield more precise human error analysis, precise human error probabilities(HEPs) must be used in the analysis. However, because human behavior is influenced by factors that are called performance shaping factors(PSFs), the effects of PSFs must be considered to obtain precise HEPs, These are called basic HEPs or situation-specific HEPs. This paper presents a theoretical method for obtaining basic HEPs (i.e. , considering PSFs) in quantitative human error analysis. In this method, the weight which characterizes the degree of importance of several PSFs is obtained by the analytic hierarchy process. The quality scores of PSFs in the task situation are obtained by percentile concept. These scores are used in conjunction with the relative Importance weights of PSFs to compute the composite quality percentile score of PSFs in the task situation. Then, a new mapping method of the composite quality percentile score of PSFs into a situation-specific basic HEP is proposed with a numerical example.

• Mass Spectrometry Letters
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• 제12권3호
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• pp.81-84
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• 2021

Collagen, which accounts for one-third of human protein, is reduced due to human aging, and much attention is focused on making collagen into food to prevent such aging. Gel permeation chromatography with Reflective Index (RI) detection (GPC/RI) was chosen as the most suitable instrument to confirm molecular weight distribution, and we explored the use of this technique for analysis of collagen peptide molecular sizes and distributions. Data reliability was verified by matrix-assisted laser desorption/ionization coupled to time-of-flight (MALDI-TOF) mass spectrometric analysis. The data were considered meaningful for comparative analysis of molecular weight distribution patterns.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2006년도 한국약용작물학회 공동춘계학술발표회
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• pp.172-173
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• 2006

• 대한조선학회논문집
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• 제57권3호
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• pp.124-132
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• 2020

The purpose of this paper is to establish the basic procedure and method for selection of preferred anti-fouling paint considering economic analysis according to ship's speed loss based on ISO 19030 that has been published in 2016 to prescribe practical methods for measuring changes in ship specific hull and propeller performance. In this study, six (6) anti-fouling paint products have been assumed for alternatives with each target maximum average speed loss and carried out comparison of the alternatives and sensitivity analysis in assumed conditions for selection of the preferred alternative.

• The International Journal of Costume Culture
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• 제8권2호
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• pp.97-104
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• 2005

In this research, the fit of basic dresses was examined, by analyzing and comparing the satisfaction of fitness of basic dresses made by the draping method on a custom-made replica body form and that of a basic dress made by the flat pattern method. Basis dresses were produced by the draping method for each 52 subjects using custom-made body forms, and another kind basic dresses for each subjects were produced by the flat pattern method using measurements of body sizes. Both basic dresses received high scores for the satisfaction of fit, but the basic dresses produced by draping method received higher scores. Thus it is expected to produce a more successful fit by draping a basic dress on a custom-made body form. Future research using more subjects might confirm these results. A more concrete analysis through using additional satisfaction tests could be conducted. For example, each subject might also serve as an evaluator to rate her satisfaction of fit wearing each of the two custom-made dresses. Finally, examining various body forms is suggested.

• Journal of Plant Biotechnology
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• 제29권3호
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• pp.161-170
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• 2002

인삼 모상근의 프로테옴 분석에 의해 얻은 질량분석 스펙트럼 데이터는 MALDI/TOF/MS에서 얻는 질량 스펙트럼과 ESI/Q-TOF/MS에서 얻는 탄뎀 질량 스펙트럼으로 구분된다. 질량 스펙트럼은 단백질이 효소에 의해 분해된 펩타이드들의 분자량 정보를 제공하며, 탄뎀 질량 스펙트럼에서는 아미노산 단위로 분해된 절편 단백질의 분자량으로부터 아미노산 서열을 결과로 얻는다. 펩타이드의 아미노산 서열을 BLAST로 검색하면 유사한 단백질을 GenBank에서 검색할 수 있다. 이러한 단백질 동정 방법은 완전한 유전체 서열이 알려진 생물체의 경우 높은 정확도로 단백질을 동정할 수 있으나, 그렇지 않은 경우는 유사한 단백질이 데이터베이스에 존재하지 않아 분석이 용이하지 않다. 본 연구에서는 질량 스펙트럼 및 절편 단백질의 아미노산 서열을 EST (expressed sequence tag) 서열과 비교하여 프로테옴 데이터와 일치하는 EST 서열을 찾아내고 이를 BLAST검색에 의해 단백질 동정에 활용하였다. ESI/Q-TOF/MS 에서 얻은 아미노산 서열은 길이는 짧지만 데이터의 신뢰도가 높으므로 EST 서열과의 연관 관계를 밝힘으로써 단백질에 대한 정보를 보완할 수 있었다. ESI/Q-TOF/MS에서 얻은 펩타이드의 아미노산 서열을 EST 서열과 비교한 결과 90%의 아미노산 서열이 EST DB에서 발견되었다. NCBI의 nr 데이터베이스에서 아미노산 서열을 검색하여 찾은 단백질이 68%임에 비하여, 인삼 EST 서열에 의한 검색이 22% 더 많은 결과를 얻었다. MALDI/TOF/MS의 질량 스펙트럼에서 nr 데이터베이스로 검색한 결과와 인삼 EST 데이터베이스를 검색한 결과가 일치하는 경우는 47개 중 9개인 19%에 불과하여, 탄뎀 질량 분석으로 아미노산 서열을 얻지 않고, 단지 질량 스펙트럼으로부터 단백질을 동정하는 방법으로는 단백질 동정의 정확한 결과를 기대하기 어려움을 확인하였다.

• Bulletin of the Korean Chemical Society
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• 제33권10호
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• pp.3298-3302
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• 2012

Although offline enrichment of phosphorylated peptides is widely used, enrichment for phosphopeptides using $TiO_2$ is often performed manually, which is labor-intensive and can lead to irreproducible results. To address the problems associated with offline enrichment and to improve the effectiveness of phosphopeptide detection, we developed an automated online enrichment system for phosphopeptide analysis. A standard protein mixture comprising BSA, fetuin, crystalline, ${\alpha}$-casein and ${\beta}$-casein, and ovalbumin was assessed using our new system. Our multidimensional system has four main parts: a sample pump, a 20-mm $TiO_2$-based column, a weak anion-exchange, and a strong cation-exchange (2:1 WAX:SCX) separation column with LC/MS. Phosphorylated peptides were successfully detected using the $TiO_2$-based online system with little interference from nonphosphorylated peptides. Our results confirmed that our online enrichment system is a simple and efficient method for detecting phosphorylated peptides.