• Bulletin of the Korean Chemical Society
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• v.24 no.8
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• pp.1163-1168
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• 2003

The structures of molecular species of galactolipids, such as monogalactosyl diacylglycerol (MGDG) and digalactosyl diacylglycerol (DGDG), isolated from wheat flour have been investigated using negative-ion electrospray ionization (ESI) mass spectrometry interfaced with high performance liquid chromatography (HPLC). According to the result of HPLC analysis, MGDG and DGDG were found to consist of mixtures of five and four molecular species, respectively. The galactolipids have been also analyzed to determine their fatty acid compositions, using HPLC/ESI-MS combined with in-source (or cone voltage) fragmentation. HPLC/ ESI-MS is very useful for one-step analysis of mixtures of galactolipids with a small sample quantity. Especially, the carboxylate anions produced in in-source fragmentations of the negative-ion of each component separated by HPLC provide valuable information on the composition of its fatty acyl chains.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2003.10a
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• pp.176-176
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• 2003

• Proceedings of the KSR Conference
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• 2007.11a
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• pp.629-632
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• 2007

Communications Based Train Control(CBTC) is a continuous, automatic train control system utilizing high-resolution, train location determination independent of track circuits; continuous, high capacity, bi-directional train-to-wayside data communications; and trainborne and wayside processors capable of implementing vital functions. In this paper, we present a basic design of CBTC system based on ERTMS/ETCS specifications. The basic design is composed of system requirements analysis, system functional analysis, system architectural design, sub-system requirements analysis, sub-system functional analysis. Each deliverable has been produced according to a development process based on UML/SysML.

• Journal of the Korean Society of Safety
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• v.12 no.1
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• pp.113-121
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• 1997

Human reliability attempts to make precise quantitative analyses and predictions of the performance of human-machine(or product) systems. In order to yield more precise human error analysis, precise human error probabilities(HEPs) must be used in the analysis. However, because human behavior is influenced by factors that are called performance shaping factors(PSFs), the effects of PSFs must be considered to obtain precise HEPs, These are called basic HEPs or situation-specific HEPs. This paper presents a theoretical method for obtaining basic HEPs (i.e. , considering PSFs) in quantitative human error analysis. In this method, the weight which characterizes the degree of importance of several PSFs is obtained by the analytic hierarchy process. The quality scores of PSFs in the task situation are obtained by percentile concept. These scores are used in conjunction with the relative Importance weights of PSFs to compute the composite quality percentile score of PSFs in the task situation. Then, a new mapping method of the composite quality percentile score of PSFs into a situation-specific basic HEP is proposed with a numerical example.

• Mass Spectrometry Letters
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• v.12 no.3
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• pp.81-84
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• 2021

Collagen, which accounts for one-third of human protein, is reduced due to human aging, and much attention is focused on making collagen into food to prevent such aging. Gel permeation chromatography with Reflective Index (RI) detection (GPC/RI) was chosen as the most suitable instrument to confirm molecular weight distribution, and we explored the use of this technique for analysis of collagen peptide molecular sizes and distributions. Data reliability was verified by matrix-assisted laser desorption/ionization coupled to time-of-flight (MALDI-TOF) mass spectrometric analysis. The data were considered meaningful for comparative analysis of molecular weight distribution patterns.

• Proceedings of the Korean Society of Crop Science Conference
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• 2006.04a
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• pp.172-173
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• 2006

• Journal of the Society of Naval Architects of Korea
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• v.57 no.3
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• pp.124-132
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• 2020

The purpose of this paper is to establish the basic procedure and method for selection of preferred anti-fouling paint considering economic analysis according to ship's speed loss based on ISO 19030 that has been published in 2016 to prescribe practical methods for measuring changes in ship specific hull and propeller performance. In this study, six (6) anti-fouling paint products have been assumed for alternatives with each target maximum average speed loss and carried out comparison of the alternatives and sensitivity analysis in assumed conditions for selection of the preferred alternative.

• The International Journal of Costume Culture
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• v.8 no.2
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• pp.97-104
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• 2005

In this research, the fit of basic dresses was examined, by analyzing and comparing the satisfaction of fitness of basic dresses made by the draping method on a custom-made replica body form and that of a basic dress made by the flat pattern method. Basis dresses were produced by the draping method for each 52 subjects using custom-made body forms, and another kind basic dresses for each subjects were produced by the flat pattern method using measurements of body sizes. Both basic dresses received high scores for the satisfaction of fit, but the basic dresses produced by draping method received higher scores. Thus it is expected to produce a more successful fit by draping a basic dress on a custom-made body form. Future research using more subjects might confirm these results. A more concrete analysis through using additional satisfaction tests could be conducted. For example, each subject might also serve as an evaluator to rate her satisfaction of fit wearing each of the two custom-made dresses. Finally, examining various body forms is suggested.

• Journal of Plant Biotechnology
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• v.29 no.3
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• pp.161-170
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• 2002

For the hairy root of Panax ginseng, we have got mass spectrums from MALDI/TOF/MS analysis and Tandem mass spectrums from ESI/Q-TOF/MS analysis. While mass spectrum provides the molecular weights of peptide fragments digested by protease such as trypsin, tandem mass spectrum produces amino acid sequence of digested peptides. Each amino acid sequences can be a query sequence in BLAST search to identify proteins. For the specimens of animals or plants of which genome sequences were known, we can easily identify expressed proteins from mass spectrums with high accuracy. However, for the other specimens such as ginseng, it is difficult to identify proteins with accuracy since all the protein sequences are not available yet. Here we compared the mass spectrums and the peptide amino acid sequences with ginseng expressed sequence tag (EST) DB. The matched EST sequence was used as a query in BLAST search for protein identification. They could offer the correct protein information by the sequence alignment with EST sequences. 90% of peptide sequences of ESI/Q-TOF/MS are matched with EST sequences. Comparing 68% matches of the same sequences with the nr database of NCBI, we got more matches by 22% from ginseng EST sequence search. In case of peptide mass fingerprinting from MALDI/TOF/MS, only about 19% (9 proteins of 47 spots) among peptide matches from nr DB were correlated with ginseng EST DB. From these results, we suggest that amino acid sequencing using tandem mass spectrum analysis may be necessary for protein identification in ginseng proteome analysis.

• Bulletin of the Korean Chemical Society
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• v.33 no.10
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• pp.3298-3302
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• 2012

Although offline enrichment of phosphorylated peptides is widely used, enrichment for phosphopeptides using $TiO_2$ is often performed manually, which is labor-intensive and can lead to irreproducible results. To address the problems associated with offline enrichment and to improve the effectiveness of phosphopeptide detection, we developed an automated online enrichment system for phosphopeptide analysis. A standard protein mixture comprising BSA, fetuin, crystalline, ${\alpha}$-casein and ${\beta}$-casein, and ovalbumin was assessed using our new system. Our multidimensional system has four main parts: a sample pump, a 20-mm $TiO_2$-based column, a weak anion-exchange, and a strong cation-exchange (2:1 WAX:SCX) separation column with LC/MS. Phosphorylated peptides were successfully detected using the $TiO_2$-based online system with little interference from nonphosphorylated peptides. Our results confirmed that our online enrichment system is a simple and efficient method for detecting phosphorylated peptides.