• 한국식품영양과학회지
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• 제12권3호
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• pp.212-218
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• 1983

토끼에 마그네슘 칼슘이 첨가된 식이를 급여하여 토끼의 liver microsomal protein에 함유된 HMG-CoA reductase의 활성과 혈청 콜레스테롤, 트리글리세리드, 마그네슘 및 칼슘의 양을 측정하여 다음과 같은 결과를 얻었다. 1. 혈청 마그네슘의 경우 대조군이 9.5mg% 인데, 마그네슘 첨가군은 평균 8.27이었고, 칼슘을 첨가하여 사육한 실험군에서 상당히 낮은 값 T-D : 3.5, T-E : 3.0, T-F : 4.0, T-G : 7.3으로 평균 4.45를 보였다. 2. 혈청 칼슘의 경우 대조군이 15.3mg %인데, 마그네슘 첨가 실험군은 평균 14.6이나 칼슘 첨가 실험군에 있어서 평균 14.1로서 큰 차이가 없었다. 3. 혈청 트리글리세리드의 경우 대조군 82.8에 비하여 마그네슘첨가 실험군은 평균 60.3이었으나, 칼슘 첨가 실험군은 평균 69.5로서 다소 높은 값을 보여 주었다. 4. 혈청 콜레스테롤의 경우 대조군이 80mg%인데 반하여, 마그네슘 첨가 실험군은 평균 64.3이었고, 칼슘 첨가 실험군은 평균 56.3으로 상당히 낮은 값을 보여 주었다. 5. microsomal protein의 HMG-CoA reductase의 활성의 경우는 대조군이 0.995nmol/min/mg 인데, 마그네슘 첨가 실험군은 평균 0.948을 보였고 칼슘 첨가 실험군은 평균 0.849를 보여 주었다.

• 한국식품영양과학회지
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• 제27권5호
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• pp.973-979
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• 1998

This study was conducted to investigate the effectiveness of pumpkin powder in the diet of experimental animals on chemically induced stomach and mammary canceers. Three weeks old male SpragueDawley rats were randomly allocated to either 1) basal diet+MNNG, 2) basal diet+MNNG+PMC, 3) 2.5% pumpkin powder supplemented diet+MNNG+PMC, or 4) 5.0% pumpkin powder supplemented diet+MNNG+PMC for stomach cancer experiment. And female Sprague-Dawley(5 weeks old) rats were randomly assigned to either 1) basal diet only, 2) basal diet+DMBA, 3) 2.5% pumpkin powder supplemented diet+DMBA, or 4) 5.0% pumpkin powder supplementd diet+DMBA. In both experiments, supplements of pumpkin powder in basal diet decreased body weight of both male and female experimental animals. Pumpkin powder in rat diet decreased significantly(p<.05) chemically induced stomach cancer. With its suppressing effects on stomach cancer, pumpkin powder in diet of experimental rats had decreasing effects on the initiation and development of DMBA-induced mammary cancer. In conclusiion, current study may provide in vivo data to develop health foods using pumpkin. Further studies, however, are essential to clarify the exact role of pumpkin powder in chemically induced stomach and mammary cancers.

• 한국응용과학기술학회지
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• 제1권1호
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• pp.3-9
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• 1984

Interaction of dietary Magnesium, Calcium and Polyunsaturated fatty acid(vegetable oils)on 3-Hydroxy-3-Methylglutaryl Co-A reductase activity was studied for a period of 30 days using isocalories and isonitrogenous as a basal diet . The subject rabbits were divided into 18 feeding groups. The results are summarized as follows: 1. The ratio of ${\alpha}_1-lipoprotein$ par lipalbumin is 0.34 for control group, 0.38 the highest group fed 0.1 Mg(II) 10ml plus perilla oil and basal diet, the lowest 0.25 group fed 0.1M Mg(II) 5ml plus sesame oil and basal diet. 2. The ratio ratio of ${\alpha}_2-lipoprotein$ per lipalbumin is 0.64 for control group. 0.95 as the highest for the group fed 0.1M Ca(II) 15ml plus sesame oil and basal diet, 0.1M Ca(II) 5ml plus perilla oil and basal diet. 3. The ratio of ${\beta}-lipoprotein$ per lipalbumin is 0.71 for control group, the highest 0.81 for the groups fed 0.1M Mg(II) 10ml plus sesame oil and basal diet, the lowest 0.37 for the group fed 0.1M Mg(II) 15ml plus soybean oil and basal diet. 4. In serum triglyceride, control group was 129.5mg%, the highest 155.4mg% for the group fed 0.1M Ca(II) 5ml plus sesame oil and basal diet, the lowest 85.7mg% for the group fed 0.1M Mg(II) 10ml plus soybean oil and basal diet. 5. In serum cholesterol, control group was 96.7mg%, the highest 152.5mg% for the group fed 0.1M Ca(II) 10ml plus sesame oil and basal diet, the lowest 80.5mg% for the group fed 0.1M Mg(II) 15ml plus soybean oil and basal diet. 6. In case of HMG-CoA reductase activity, control group was 0.95, the highest 0.98 for the group fed 0.1M Ca(II) plus soybean oil and basal diet. 7. Interaction between metal(II) ions and polyunsaturated fatty acid(vegetable oil) are soybean oil>sesame oil>perilla oil, for Mg(II). soybean oil>perilla oil>sesame oil, for Ca(II). Therefore, it is invetigated that the interaction between metal ion and polyunsaturated fatty acid is the higher, the cholesterol level is the lower, and HMG-CoA reductase activity is increased.

• Asian-Australasian Journal of Animal Sciences
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• 제21권6호
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• pp.838-844
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• 2008

This experiment was conducted to evaluate the effect of supplementing a fat diet with selenium (Se) and vitamin E on performance of cows, blood antioxidant status and milk fatty acid composition. Sixty-three lactating Holstein cows were randomly divided into seven groups of nine cows each and each group was fed one of the following diets: i) a basal diet (control); ii) a basal diet with 0.15 mg Se/kg DM (LSe); iii) a basal diet with 0.3 mg Se/kg DM (HSe); iv) a basal diet with 5,000 IU/cow d vitamin E (LVE); v) a basal diet with 10,000 IU/cow d vitamin E (HVE); vi) a basal diet with 0.15 mg Se/kg DM and 5,000 IU/cow d vitamin E (LSeVE); vii) a basal diet with 0.3 mg Se/kg DM and 10,000 IU/cow d vitamin E (HSeVE). Milk fat percentage and conjugated linoleic acid (CLA) yield in HVE and HSeVE diets increased (p<0.05) compared with the control diet. In milk fat, dietary supplementation of Se tended to increase the proportion of the sum of unsaturated fatty acids (UFA) and significantly decreased (p<0.05) the proportion of the sum of saturated fatty acids (SFA). In addition, compared with the control, thiobarbituric acid reactive substances (TBARS) content was lower and glutathione peroxidase (GSH-Px) was higher when fat diets were supplemented with Se. Our data showed that supplementation with Se and/or VE improved these nutrients in blood and milk. The results indicated that fat diets supplemented with Se improved both antioxidant status in blood and fatty acids in milk fat, and fat diets supplemented with vitamin E alleviated milk fat depression. Therefore, the combination of Se and vitamin E caused synergistic effects on the nutritional quality of milk fat and performance of cows fed a fat diet.

• Journal of Nutrition and Health
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• 제34권4호
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• pp.433-439
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• 2001

Intakes and excretions of calcium and phosphorus were determined for 8 female adolescents(aged 16.3$\pm$0.5y; body mass index 20.4$\pm$1.3kg/$m^2$; body fat 33.3$\pm$2.5%; bone mineral density of lumbar spine in L2-L4; 0.96$\pm$0.08g/$\textrm{cm}^2$) when they consumed diets basal and high in calcium for 6 days each. All subjects consumed a basal Ca diet containing 800mg, Korean RDA level of the subjects, and a high Ca diet containing 1200mg, RDA plus 2 SDs of calcium intake. The diets provided 58% of energy intake as carbohydrate, 25% as fat, and 17% as protein. Food, urine, and fecal composites were collected during the last 3 days of each feeding period. Fecal excretion of calcium was significantly greater on the high Ca than on the basal Ca diet. Hence, apparent absorption rate was significantly lowered from 40.9% on the basal Ca diet to 33.1% on the high Ca diet. There was no significant difference in calcium retention between the two diet periods but it tended to be greater on the high Ca diet. However, excretions and retention of phosphorus did not show any significant difference even though intake was significantly increased from 1,253mg on the basal Ca diet to 1,583mg on the high Ca diet. The results indicate that higher calcium intake than the Korean RDA level is recommended for adolescents to meet peak bone mineral accretion and attain a desirable level of calcium retention. (Korean J Nutrition 34(4) : 433~439, 2001)

• Journal of Animal Science and Technology
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• 제47권4호
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• pp.501-512
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• 2005

본 시험은 약용식물(인진쑥, 오가피, 마늘)의 사료내 첨가가 모돈의 생산성, 포유자돈의 성장율 및 이유자돈에서의 성적 및 혈청 특성에 미치는 영향을 알아보고자 실시하였다. 시험 1은 3~5산차의 임신 모돈 48두를 공시하여 각각 1) CON(basal diet; Control), 2) MP1(basal diet added 0.05% of medicinal plant mixtures), 3) MP2(basal diet added 0.1% of medicinal plant mixtures) 그리고 4) MP3(basal diet added 0.2% of medicinal plant mixtures)로 하여 처리구당 12두씩 배치하여 21일동안 실시하였다. 시험 기간동안 포유모돈의 일당 사료섭취량은 약용식물을 0.1% 첨가한 처리구가 0.2%를 첨가한 처리구보다 유의적으로 높게 나타났다(P<0.05). 포유자돈의 개시시와 이유시의 체중 변화에서는 약용식물을 0.05% 첨가한 처리구가 대조구에 비하여 유의적으로 증가하는 경향을 보였다 (P<0.05). 혈액내 glucose의 함량에서는 MP3 처리구가 대조구에 비하여 유의적으로 높게 나타났다(P<0.05). 시험 2는 개시시 체중 4.70$\pm$0.63kg의 3원교잡종(Landrace$\time$Yorkshire$\time$Duroc) 이유자돈 120두를 공시하여 20일간 사양시험을 실시하였다. 시험설계는 옥수수－대두박 위주의 사료로서 NRC(1998)의 영양소 요구량에 따라 1) CON(basal diet; Control), 2) MP1(basal diet added 0.05% of medicinal plant mixtures), 3) MP2(basal diet added 0.1% of medicinal plant mixtures) 그리고 4)MP3(basal diet added 0.15% of medicinal plant mixtures)의 4개의 처리구로 하여 처리당 6반복, 반복당 5두씩 완전임의 배치하였다. 전체시험기간 동안의 이유자돈의 일당증체량(linear, P<0.067)과 사효효율(linear, P<0.018)에서는 약용식물을 첨가함으로서 증가하는 경향을 보였으며, 사료섭취량(linear, P<0.018)에서는 감소하는 경향을 보였다. 건물의 소화율에서는 약용식물을 첨가함에 따라 증가하는 경향을 보였다(linear, P<0.004; quadratic, P<0.03). 결론적으로 포유모돈 사료내 약용식물의 첨가는 0.1%까지 첨가 급여시가 모돈의 체손실 감소 및 포유자돈의 증체량의 향상을 가져 왔으며, 이유자돈에서는 약용식물을 0.05-1.0% 첨가시 자돈의 성장율 및 건물의 소화율을 개선시키는 결과를 보였다.

• 동의생리병리학회지
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• 제18권1호
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• pp.187-193
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• 2004

Constipation is a common clinical problem that comprises of symptoms that include excessive straining, hard feces, feeling of incomplete evacuation and infrequent defecation. Although many conditions, such as metabolic problems, fiber deficiency, anorectal problem, an drug, can cause constipation. This study was examined the effects of Chilsun-Whan on intestinal mucosa and gastrointestinal transit time and plasma lipids in rats. Adult male rats were fed for weeks on diets containing no addition(basal diet group), 5% cellulose(cellulose group) and 2.5% . Chilsun-Whan group(Chilsun-Whan group). The results were as follows; 1. The fecal weght was significantly increased 2 times in Chilsun-Whan administrated group compare to basal diet group. 2. The gastrointestinal transit times was significantly decreased in Chilsun-Whan administrated group compare to basal diet. 3. Carmine red mixed with Chilsun-Whan, as a marker, was administered through a gastric tube for stomach or intracecally by a chronically implanted catheter for colon transit. Small intestinal transit and large intestinal transit time were significantly decreased in Chilsun-Whan administrated group compare to basal diet. 4. The height of jejunal villi was developed in Chilsun-Whan administrated group compare to basal diet The thickness of mucosa and muscle layer of colonic mucosa were significantly developed in Chilsun-Whan administrated group compare to basal diet group. 5. The change of goblet cell in colonic mucosa was increased acid mucin stained alcian blue in Chilsun-Whan administrated group compare to basal diet and cellulose group. 6. HDL-cholesterol of plasma lipid was increased in Chilsun-Whan administrated group compare to basal diet and cellulose groups. Theses results suggests that Chilsun-Whan may be used in prevention and treatment of constipation resulting in increase of fecal weight, decrease of gastrointestinal transit time. development of intestinal villi, intensify of stainability of acid mucin in colon.

• Toxicological Research
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• 제14권4호
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• pp.535-539
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• 1998

The present study was designed to investigate the serum cholesterol lowering effect oj the phytosterol derivative (LPSS) on high cholesterol (HC) diet-induced hypercholesterolemia in male weaning Sprague-Dawley (SD) rats. Rats were fed with HC diet containing 1 % cholesterol and 0.5% cholic acid for 1 week. After 1 week, the LPSS oil suspension (0.32 g/kg B. W.) was orally administered to the rats fed with either basal diet or HC diet groups for 7 days. In addition, the LPSS powder (0.14%) mixed with basal diet or HC diet was Jed to the rats for 7 days. Serum total cholesterol and LDL-cholesterol contents were not altered by administration of the LPSS oil suspension with basal diet. However, they were significantly decreased by administration of the LPSS oil suspension with HC diet at day 14. Also, they were significantly decreased by the LPSS powder mixed with basal diet or HC diet at day 9, 11, 14. HDL-cholesterol contents were not altered by the LPSS oil suspension or LPSS powder. These results indicated that the phytosterol derivative(LPSS) might decrease serum total cholesterol and LDL-cholesterol contents in rats.

• Journal of Animal Science and Technology
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• 제66권1호
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• pp.103-114
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• 2024

This study was done to investigate the effects of the incorporation of Achyranthes japonica extracts (AJE) in diet on the production parameters of growing pigs. Exp 1: Total, 105 crossbred pigs (average body weight: 24.47 ± 2.46 kg) were used in a 6-week feeding trial. Pigs (seven replicates, five pigs per pen) were allotted randomly to three treatments. Dietary treatments: CON (basal diet); basal diet with 0.025% AJE, and basal diet + 0.050% AJE). Growth performance, nutrient digestibility, fecal microbial count, and fecal noxious gas were assessed in this study. Average daily gain (ADG), average daily feed intake (ADFI), and gain to feed ratio (G:F) were not affected by the addition of up to 0.05% AJE. In the case of apparent total tract digestibility (ATTD), dry matter (DM), nitrogen (N), and digestible energy (DE) were not changed in 3rd and 6th weeks of the feeding trial through the addition of AJE up to 0.05% in the growing pig diet. In microbial count, Lactobacillus and Escherichia coli count at 3rd and 6th week was similar in all the treatment diets. The inclusion of AJE at levels up to 0.05% in growing pig diet had no effect on the production of NH3, H2S, acetic acid, and CO₂ in the feces. After ending the Exp 1, a total of nine pigs were divided into three treatment groups. Treatment diets were included, TRT1, basal diet + powder quercetin 30 g; TRT2, basal diet + powder quercetin 150 g; TRT3, basal diet + powder quercetin 300g. Rate of absorption in blood was increased with the higher dose of quercetin. The results suggested incorporation of AJE up to 0.05% has no significant effect on ADG, ADFI, and G:F, as well as DM, N, and DE digestibility, fecal microbial count, and fecal noxious gas emission in growing pigs, even though no negative effect was found.

• Journal of Nutrition and Health
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• 제33권5호
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• pp.502-506
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• 2000

Effects of powdered siho(bupleuri radix) on serum and liver lipid composition and antioxidative capacity were investigated in rat fed high oxidized fat. Sixty male Sprague-Dawley rats weighing 161.25$\pm$2.51g were blocked into four groups according to body weight and raised seven weeks with basal diet(normal group), basal diet and 10% oxidized fat(control group), basal diet, 10% oxidized fat and 2% powdered siho(2% powdered siho group) and basal diet, 10% oxidized fat and 3% powdered siho(3% powdered siho group). Feed intake, body weight gain and feed efficiency were not significantly different among oxidized fat diet groups. The values of total cholesterol and triglyceride in plasma and liver showed no significantly different(p>0.05) in the oxidized fat diet groups. Plasma HDL-cholesterol showed atendency to increase in powdered siho diet groups. The values of thiobarbituric acid in plasma and liver were lower in the powdered siho diet groups than control group. Plasma GOT and GPT activity showed a tendence to decrease in powdered siho groups. Liver glutathione peroxidase activity showed a tendence to increase in powdered siho diet groups. Thus, it seems that powdered siho had some antioxidative elements.