• Journal of Korean Society of Forest Science
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• v.107 no.2
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• pp.193-204
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• 2018

This study was carried out to select the optimal method of competition index computation according to the competitor selection methods and distant-dependent competition index models, and to analyze the characteristics of competition indices in terms of thinning intensity and tree density targeting Pinus densiflora, Pinus koraiensis, and Larix kaempferi, which are the major coniferous species in Korea. Data was the re-investigated tree information from 240 permanent plots of 80 sites in the stands of P. densiflora, P. koraiensis, and L. kaempferi, which were located in the national forest of Gangwon and North Gyeongsang provinces. The number of subject trees with competition index calculated were 1126 trees for P. densiflora, 4093 trees for P. koraiensis, and 3399 trees for L. kaempferi. For the best competition index computation method, three kinds of competitor selection methods were considered: basal area factor, angle of height, angle of height to crown base. Also, six kinds of competition index models were compared: Lorimer, Martin-EK, Braathe, Heygi, Daniels, and Modified Daniels, which was developed in this study. Correlation coefficient was the best when the competitor selection method of basal area factor $4m^2/ha$ and the competition index model of Modified Daniels were used, and thus, it was selected as the best method for computing competition index. According to the best method by stand characteristics, competition index decreased in all species as thinning intensity was high and tree density was low.

• The Journal of Korean Medicine
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• v.31 no.3
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• pp.1-7
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• 2010

Objectives: To introduce to TKM scientific dose conversion methods of human to animal or animal to human for new drug investigations. Methods: We searched guidelines of the FDA and KFDA, and compared them with references for drug-dose conversion from various databases such as PubMed and Google. Then, we analyzed the potential issues and problems related to dose conversion in safety documentation of new herbal drugs based on our experiences during Investigational New Drug (IND) applications of TKM. Results: Dose conversion from human to animal or animal to human must be appropriately translated during new drug development. From time to time, investigators have some difficulty in determining the appropriate dose, because of misunderstandings of dose conversion, especially when they estimate starting dose in clinical or animal studies to investigate efficacy, toxicology and mechanisms. Therefore, education of appropriate dose calculation is crucial for investigators. The animal dose should not be extrapolated to humans by a simple conversion method based only on body weight, because many studies suggest the normalization method is based mainly on body surface area (BSA). In general, the body surface area seems to have good correlation among species with several parameters including oxygen utilization, caloric expenditure, basal metabolism, blood volume and circulating plasma protein. Likewise, a safety factor should be taken into consideration when deciding high dose in animal toxicology study. Conclusion: Herein, we explain the significance of dose conversion based on body surface area and starting dose estimation for clinical trials with safety factor.

• Journal of Korean Society of Forest Science
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• v.90 no.5
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• pp.650-662
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• 2001

The growth and yield models for five different kinds of natural forest types were systemically developed in the natural Broadleaved-Korean pine Forests in Northeast China. The data were collected from 359 temporary plots and 58 permanent plots with area ranged from 0.06 ha to 1.0 ha, ranging in stand age from 43 to 364 years. The Site Class Index (SCI) was introduced to evaluate site quality and the Crown Competition Factor (CCF) was selected as a measure of stand density for the mixed natural forest. The Chapman-Richards function was adopted to develop SCI equation and height-diameter curve. The Schumacher growth function was selected as base model to develop the DBH, basal area, and stand volume growth models by using re-parameterized method. In modeling mean DBH and basal area growth, it was found that the asymptotic parameter A of Schumacher function was exponentially related to site quality (SCI) and stand density (CCF). The rate parameter k was related to stand density and it was independent of SCI. Several validation measures for predicted stand variables were evaluated in the growth and yield models using independent data sets. The results indicated that relative mean errors (RME) in predicted stand attributes were less than ${\pm}5%$ and the estimated precision values of the stand variables were all greater than 95%.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.33 no.3
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• pp.191-198
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• 2007

The p53 which is well known as tumor suppressor gene is located at 17p13. p53 is a sequence-specific DNA binding transcription factor that responds to certain cytotoxic stresses, such as DNA damage, by enhancing the transcription of genes that regulate cell-cycle progression as well as programmed cell death. The p63 gene that is located at 3q27-29, is recognized members of the p53 family, and responsible for the transcription of 6 isoforms. Three isoforms ($TAp63{\alpha}$, $TAp63{\beta}$, $TAp63{\gamma}$) contain an N-terminal transactivation (TA) domain and can induce apoptosis. The other 3 isoforms (${\Delta}Np63{\alpha}$, ${\Delta}Np63{\beta}$, ${\Delta}Np63{\gamma}$) lack the TA domain and may function in a dominant-negative fashion by inhibiting the transactivation functions of p53 and TAp63 proteins, and thus act as oncoproteins. A number of studies have investigated the role of p63 in human squamous cell carcinomas from different organs. Only a few studies have examined ${\Delta}Np63$ isoform in oral squamous cell carcinoma including normal epithelium. This study aimed to evaluate expression of ${\Delta}Np63$ isoform in human oral squamous cell carcinoma tissue and normal mucosa. The 3 cases of well differenciated oral squamous cell carcinoma specimen including adjacent normal mucosa were examined, and immunohistochemical study with monoclonal antibody(4A4) and tumor cell apoptosis analysis with Transmission Electon Microscopy were studied. And, RT-PCR analysis was done for expression of ${\Delta}Np63$ isoform. The results were as followed. 1. Normal gingiva showed the restricted p63 expression in basal cell layer. 2. Well differentiated squamous cell carcinoma showed mainly p63 expression in overall area of malignancy, especially in basal cell layer to adjacent stromal tissue. 3. Tumor cells around keratinized area with no p63 expression disclosed less micro-organelle in decreased size cytoplasm and severe chromatin margination with nuclear destruction that means apoptosis. 4. Comparison of mRNA expression of ${\Delta}Np63$ isoform by RT-PCR showed variable expression of ${\Delta}Np63$ isoform, but ${\Delta}Np63{\alpha}$ was most highly expressed in all 3 tumor specimen. From theses results, it should be suggested that ${\Delta}Np63$ isoform expression in well differentiated squamous cell carcinoma was closely related to tumor oncogenesis, expecially overexpression of ${\Delta}Np63{\alpha}$ is a most important factor in tumor genesis of oral squamous cell carcinoma.

• Journal of Ecology and Environment
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• v.44 no.4
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• pp.264-274
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• 2020

Background: Climate change has altered the various ecosystem processes including forest ecosystem in Himalayan region. Although the high mountain natural forests including treelines in the Himalayan region are mainly reported to be temperature sensitive, the temperature-related water stress in an important growth-limiting factor for middle elevation mountains. And there are very few evidences on growth performance of planted forest in changing climate in the Himalayan region. A dendrochronological study was carried out to verify and record the impact of warming temperature tree growth by using the tree cores of Pinus roxburghii from Batase village of Dhulikhel in Central Nepal with sub-tropical climatic zone. For this total, 29 tree cores from 25 trees of P. roxburghii were measured and analyzed. Result: A 44-year long tree ring width chronology was constructed from the cores. The result showed that the radial growth of P. roxburghii was positively correlated with pre-monsoon (April) rainfall, although the correlation was not significant and negatively correlated with summer rainfall. The strongest negative correlation was found between radial growth and rainfall of June followed by the rainfall of January. Also, the radial growth showed significant positive correlation with that previous year August mean temperature and maximum temperature, and significant negative correlation between radial growth and maximum temperature (Tmax) of May and of spring season (March-May), indicating moisture as the key factor for radial growth. Despite the overall positive trend in the basal area increment (BAI), we have found the abrupt decline between 1995 and 2005 AD. Conclusion: The results indicated that chir pine planted population was moisture sensitive, and the negative impact of higher temperature during early growth season (March-May) was clearly seen on the radial growth. We emphasize that the forest would experience further moisture stress if the trend of warming temperatures continues. The unusual decreasing BAI trend might be associated with forest management processes including resin collection and other disturbances. Our results showed that the planted pine forest stand is sub-healthy due to major human intervention at times. Further exploration of growth climate response from different climatic zones and management regimes is important to improve our understanding on the growth performance of mid-hill pine forests in Nepal.

• Animal Bioscience
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• v.37 no.8
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• pp.1367-1376
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• 2024

Objective: Parathyroid hormone like hormone (PTHLH), as an essential factor for bone growth, is involved in a variety of physiological processes. The aim of this study was to explore the role of PTHLH gene in the growth of antlers. Methods: The coding sequence (CDS) of PTHLH gene cDNA was obtained by cloning in sika deer (Cervus nippon), and the bioinformatics was analyzed. The quantitative real-time polymerase chain reaction (qRT-PCR) was used to analyze the differences expression of PTHLH mRNA in different tissues of the antler tip at different growth periods (early period, EP; middle period, MP; late period, LP). Results: The CDS of PTHLH gene was 534 bp in length and encoded 177 amino acids. Predictive analysis results revealed that the PTHLH protein was a hydrophilic protein without transmembrane structure, with its secondary structure consisting mainly of random coil. The PTHLH protein of sika deer had the identity of 98.31%, 96.82%, 96.05%, and 94.92% with Cervus canadensis, Bos mutus, Oryx dammah and Budorcas taxicolor, which were highly conserved among the artiodactyls. The qRT-PCR results showed that PTHLH mRNA had a unique spatio-temporal expression pattern in antlers. In the dermis, precartilage, and cartilage tissues, the expression of PTHLH mRNA was extremely significantly higher in MP than in EP, LP (p<0.01). In the mesenchyme tissue, the expression of PTHLH mRNA in MP was significantly higher than that of EP (p<0.05), but extremely significantly lower than that of LP (p<0.01). The expression of PTHLH mRNA in antler tip tissues at all growth periods had approximately the same trend, that is, from distal to basal, it was first downregulated from the dermis to the mesenchyme and then continuously up-regulated to the cartilage tissue. Conclusion: PTHLH gene may promote the rapid growth of antler mainly through its extensive regulatory effect on the antler tip tissue.

• Journal of Korean Society on Water Environment
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• v.36 no.5
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• pp.423-430
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• 2020

To improve the low treatment efficiency of sewage treatment plants, the separated sewer system must be maintained to provide an adequate flow rate and quality of the sewage under the effect of inflow. In this study, data from five locations of Namsuk, Dukgok1, Dukgok2, Kanggu, and Opo were used to conservatively calculate the inflow water volume. The sewer flow and rainfall data were collected in 2017. The factors in the standard method used to calculate the inflow of the combined sewer pipes including "rainy days", "rainfall impact period", and "period for basal sewer" were defined as 3 mm/day, continuous rain for two days, and two weeks prior to the inflow generation, respectively. "Rainy days", "rainfall impact period", and "period for basal sewer" were conservatively adjusted to 5 mm/day, continuous rain for five days, and three weeks prior to the inflow generation, respectively. As a results of the adjustment, the linearity (r²) was improved except for in Dukgok1. This implies that the conservative adjustment made in this study could improve the management quality of sewer pipes. Also, the linear correlation coefficient (a_i) between inflow and rainfall showed a large difference between the target locations, which can be another monitoring factor affecting the quality of sewer pipes. To improve the correlation based on the individual characteristics of the locations in Korea, the automatic algorithm for the inflow calculation should be developed by innovative intellectual technologies for application to the entire national area.

• Journal of Korean Society of Forest Science
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• v.77 no.2
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• pp.186-192
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• 1988

This study was carried out to estimate the stand form factor that is necessary to estimate stand volume by STRAND method among plotless sampling methods. The data measured for this study were based on the 380 sample plots from Larix leptolepis and 358 sample plots from Pinus koraiensis which were distributed in the region of Kyeongi, Kangweon, Chungbuk, Chungnam, Chun-buk and Kyeongbuk. 1. Stand form factor was highly correlated with in the following order, stand form height, stand average height, average diameter, stand age, distance of stem, and basal area height. 2. The best fitted equation of stand form factor of above two tree species were presented in table 3. 3. Stand form factor tables using estimated equations on the table 3 were prepared and presented in table 4, 5(Larix leptolepis), 6, 7(Pinus karaiensis). 4. The relatioinships between estimated value and actual value were Y=bx, where b approached nearly 1.0, and there were not any significant differences between them. 5. The percentages of estimated error on stand form factor table were ranged from 2.39% to 4.15% in Larix leptolepis and from 1.73% to 2.50% in Pinus koraiensis. Therefore, the stand form factor could be exactly estimated by use of these tables.

• Journal of Physiology & Pathology in Korean Medicine
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• v.19 no.1
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• pp.92-97
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• 2005

Inhibition of inflammatory response, acceleration of basal cell growth, and balanced synthesis of extracellular matrix (ECM) are important in healing of cutaneous open wounds. In order to evaluate the healing effects of water extracts of Radix Astragali (the root of Astragalus membranaceus (Fisch.)) on open wound at early stage, the experimental open wounds were generated on the dorsal sides of SD rats under anesthesia. The boiled-water extracts of Radix Astragali $(100{\mu}l)$, soaked into an occlusive film dressing were applied once a day for eleven consecutive days. The healing process was assessed by measuring macroscopic appearance and wound areas of the open wounds. The molecular aspects of healing process by Radix Astragali extracts were also investigated by Hematoxylin-Eosin (H-E) double staining and immunohistological staining of collagen type I in the healed skin area, implying cell density and linear alignment of the granulation tissue, and ECM synthesis and its remodeling, respectively. The Astragali radix extracts were found to significantly accelerate the cutaneous wound healing by suppressing the inflammation and stimulating the basal cell growth in wounded area, as compared to epidermal growth factor (EGF).

• Animal cells and systems
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• v.3 no.3
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• pp.311-317
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• 1999

Prolactin (PRL) was reported to be locally synthesized in many brain areas including the hypothalamus, thalamus (TH) and hippocampus (HIP). In the pituitary lactotrophs, PRL synthesis is dependent upon a pituitary-specific transcription factor, Pit-1. In the present study, we attempted to identify Pit-1 or Pit-1-like protein in brain areas known as the synthetic sites of PRL. Reverse transcription-polymerase chain reaction (RT-PCR) and Northern blot analysis showed the same Pit-1 transcripts in brain areas such as the medial basal hypothalamus (MBH), preoptic area (POA), TH, and HIP with the Pit-1 transcripts in the anterior pituitary (AP). Electrophoretic mobility shift assay (EMSA) was run with nuclear protein extracts from brain tissues using a double strand oligomer probe containing a putative Pit-1 binding domain. Shifted bands were found in EMSA results with nuclear proteins from MBH, POA, TH and HIP. Specific binding of the Pit-1-like protein was further confirmed by competition with an unlabeled cold probe. Antisense Pit-1 oligodeoxynucleotide (Pit-1 ODN), which was designed to bind to the Pit-1 translation initiation site and block Pit-1 biosynthesis, was used to test Pit-1 dependent brain PRL transcription. Two nmol of Pit-1 ODN was introduced into the lateral ventricle of a 60-day old male rat brain. RNA blot hybridization and in situ hybridization indicated a decrease of PRL mRNA signals by the treatment of Pit-1 ODN. Taken together, the present study suggests that Pit-1 may play an important role in the transcriptional regulation of local PRL synthesis in the brain.