• Korean Journal of Veterinary Research
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• v.37 no.1
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• pp.87-101
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• 1997

The development of trachea in fetuses on 60, 90 and 120 days of gestation and neonates of Korean native goats was investigated by microscopy and scanning and transmission electron microscopy. The results were summarized as follows; Light microscopic studies: 1. In the 60-day-old fetuses, the tracheal walls were differentiated and divided into four layers of the mucosa, submucosa, muscle and cartilage, and adventitia. The tracheal epithelium is composed of stratified ciliated columnar epithelium at 60- and 90-day-old fetuses while the epithelium observed at 120-day-old fetuses was pseudostraified ciliated colummar epithelium. 2. In the 90-day-old fetuses, tracheal glands extended into the submucosa and peripheral area of the tracheal cartilage. The blood vessels were observed in the submucosa and adventitia. The elastic and collagenous fibers were observed in the tracheal walls. 3. In the neonates, the tracheal walls consisted of mucosa with well-developed folds, submucosa, tracheal glands, muscle and cartilage, collagenous and elastic fibers, and adventitia, which were more developed than those of 120-day-old fetuses. The tracheal epithelium was developed as that in adults. Scanning electron microscopic studies: 4. In the 60-day-old fetuses, most of tracheal epithelial cells were nonciliated but short microvilli were sporadically observed on the luminal surface. On rare occasions, a few cells have solitary cilium. 5. In the 90-day-old fetuses, the ciliated cells appeared increasingly and cilia elongated longer than those of 60-day-old fetuses. 6. In the 120-day-old fetuses, the nonciliated cells covered with microvilli in dome-shape were barriered by thick carpet of cilia. The nonciliated cells also have many papillary projectons on the apical surface. 7. In the neonates, the nonciliated cells in tracheal epithelium were covered compactly with numerous cilia, and many secretory droplets were found on the cilia. Transmission electron microscopic studies: 8. In the 60-day-old fetuses, nonciliated cells of the tracheal epithelium contain large amounts of glycogen granules in the supernuclear and subnuclear areas meanwhile a few cell organelles were formed. Cilia were well formed along the apical cell membranes of the ciliated cells. Also found in the ciliated cells were basal corpuscles, mitochondria and short chains in granular endoplasmic reticulum(GER). Between the epithelial cells presented were well-defined junctional complex with zonula occludens and desmosomes. The nuclei were variable in size and shape. The more developed nucleoli were observed conspicuosly. 9. In the 90-day-old fetuses, nonciliated cells contained large glycogen granules. Accumulated glycogen granules were observed in the subnuclear and supranuclear portion of the cytoplasm. A few short microvilli were covered with glycocalyx. Ciliated cells contained numerous mitochondria and short chains of GER. 10. In the 120-day-old fetuses, the ciliated cells contained numerous mitochondria, abundant short chains of GER and nucleoli. Nonciliated cells contained some Golgi complex and mitochondria. The cell borders were well-defined and distinct junctional complex with zonula occludens, desmosomes, and interdigitorum. 11. In the neonates, well-developed goblet cells were observed in the tracheal epithelium. Ultrastructures of ciliated and nonciliated cells on the tracheal epithelia were similar in pattern as those in adults.

• Journal of Life Science
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• v.17 no.1 s.81
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• pp.97-104
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• 2007

Present study aims to investigate the effect of muddy water on gill and kidney tissues and blood components of Opsariichthys uncirostris amurensis in Imha reservoir. As a result of observing the gill tissues in muddy water with light and scanning electron microscopes, the edema, the exfoliation of epithelial cells and fusion of the secondary lamellae were observed. The space between gill lamellae was irregular, and a lots of muddy debris around the secondary lamellae were observed. In case of kidney tissues, the size of glomerulus was smaller and thickness of basal membrane was broader and the empty space in Bowman's capsule was wider than those of the control. The blood cells of O. uncirostris amurensis in muddy water were increased in number. Of the white blood cells, acidophilic granulocytes were highly distributed, and the shapes were irregular. Among the plasma components, the concentration of TP, ALB, A/G ratio, TG, CHOL, and AST were low, whereas that of the inorganic materials was high. Based on the above results, it is considered that muddy water possibly involves in the decrease of respiratory and excretory rates, giving rise to secondary lesion of tissues.

• Korean Journal of Plant Tissue Culture
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• v.26 no.2
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• pp.115-119
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• 1999

The single node cuttings of sweet potato (cv. Mokpo #29) plantlets maintained in vitro were cultured with (MF+) or without membrane filter (MF-) under photomixotrophic (PM), hetrotrophic (HT) and autotrophic (AT) conditions. Shoot length was the greatest (11.9cm) in 3$0^{\circ}C$ (HT) treatment and it was the shortest (3.4 cm) in $25^{\circ}C$ (PM) treatment. Nodal explants cultured in 3$0^{\circ}C$ treatment looked more vigorous than those of $25^{\circ}C$ in appearance, and node number was the greatest (10.5 per plantlet) among the treatments. But plantlets grew in 3$0^{\circ}C$ (HT) treatment were observed all overgrown. The size in leaf area was about 2 times greater and shoot length was about 2 times shorter in PM than in HT condition. Percent dry matter of shoots was 5.9% (HT) and 7.4% (PM) in $25^{\circ}C$ treatment and 6.1% (HT) and 7.4% (PM) in 3$0^{\circ}C$ treatment. Plantlets cultured in the MF+ treatments were less succulent than those cultured in the MF- treatment. Vitrified plantlets were examinated 14.8% (both $25^{\circ}C$ and 3$0^{\circ}C$) in PM condition and 22.2% ($25^{\circ}C$) and 31.5% (3$0^{\circ}C$) in HT condition. Sucrose was necessary for the survival of in vitro plantlets. In the sucrose-free medium, explants cultured in the MF- had turned yellow and were dead after 30 days of culture. But explants cultured in the MF+ were alive and produced plantlets with shoot and root (AT). On the other hand, the survival of explants on the MS basal medium (sucrose-free and hormone-free) depended entirely upon the MF attachment.

• Journal of Korean Society of Forest Science
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• v.99 no.3
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• pp.397-403
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• 2010

This study was conducted to investigate the changes in soil temperature, soil moisture content, light availability (photosynthetically active radiation, PAR) and diameter growth in 46-year-old Korean pine (Pinus koraiensis Siebold et Zuccarini) plantation located in Gwangneung experimental forest after 4 years from thinning. Three different thinning intensities [control, 45%(T45), and 60%(T60) basal area reduction] were applied in 2004. Mean soil temperature and soil water content were $8.9^{\circ}C$ and 14.3% for control, $10^{\circ}C$ and 16.1% for T45 and $10.2^{\circ}C$ and 16.1% for T60, respectively. Mean soil temperature and moisture content were significantly different among the treatment plots (p<0.05). Mean PAR in control, T45 and T60 was 44, 143 and178 ${\mu}mol/m^2$/sec, respectively and the differences were also statistically significant among the treatment plots (p<0.05). Mean annual diameter growth in control, T45 and T60 was 0.4, 1.5 and 1.2 mm, respectively. Diameter growth of Pinus koraiensis was steadily increased after thinning in comparison to control. Consequently, the results suggested that soil temperature, soil moisture content and light availability were likely to last beyond the 4 years after thinning in this Korean pine plantation.

• Journal of Korean Society of Forest Science
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• v.94 no.6
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• pp.431-440
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• 2005

Location, height and clear-length of individual tree can be measured directly by LiDAR Remote Sensing, and dbh(diameter at breast height) can be estimated indirectly by tree height measured by LiDAR. In addition, stand volume and stand biomass are computed from estimated growth factors. In this study, each estimated growth factor was compared to the field measurements to validate accuracy. The coefficient of determination of total tree heights was 0.66 for total trees, 0.68 for Pinus koraiensis, 0.66 for Larix leptolepis and 0.60 for Quercus spp. The coefficient of determination of clear-length was 0.79 for total trees, 0.73 for Pinus koraiensis, 0.79 for Larix leptolepis, 0.68 for Quercus spp. The coefficient of determination of dbh predicted was 0.73 for Pinus koraiensis, 0.73 for Larix leptolepis and 0.85 for Quercus spp. Moreover The coefficient of determination of basal area was 0.82 for Pinus koraiensis, 0.92 for Larix leptolepis and 0.95 for Quercus spp. Biomass per ha computed by growth factor using LiDAR was 40,306 dm/ha for Pinus koraiensis, 94,150 tdm/ha for Larix leptolepis and 94,481 tdm/ha for Quercus spp. by species.

• Journal of Korean Society of Forest Science
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• v.71 no.1
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• pp.40-44
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• 1985

This study was carried out to know the difference in growth and degree of competition between individuals of thinned and unthinned larch stands. Annual increment and total growth were obtained from the cores sampled at breast height. The correlation between adjacent individual trees was investigated. 1) The correlation between radius growth and basal area was used to estimated the degree of competition among individual trees. 2) In estimating the degree of competition the correlation between two individual trees was better method than others. 3) Up to 9 years after planting no competition between individual trees occurred and the Night positive correlation was shown. 4) High degree of competition was observed at age of 10 years after the planting, as the correlation coefficient was getting smaller. 5) The degree of competition among individual trees increased with increase of the growth of total stand.

• Journal of Korean Society of Forest Science
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• v.103 no.1
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• pp.23-29
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• 2014

Open- to closed canopy stage and it's ecological characteristics in vegetation succession are commonly described, but poorly understood in Korea. Vegetation development on structure, environment and understory abundance were studied for 16 yr in post-clearcut Pinus densiflora forests in the southern Gangwon-do province by applying space-for-time approach. We sampled 210 plots (10 for structure and 200 for understory) for four seral stages (1yr, 3yr, 10yr and 16yr). After clear-cut, mean stem density increased gradually to $5,714{\pm}645$ stems/ha after 16 years and mean basal area was also from $5.5{\pm}0.7m^2/ha$ after 10 years and doubled at $10.0{\pm}1.6m^2/ha$ in 16 years. Woody debris and bared soil on the forest floor peaked at 11--- after 10 years and at 10.3--- after 3 years, respectively. In understory mean cover declined with all growth form groups following succession, but in richness, forb specie increased with structural development during 16 years. Our study suggested that overstory development did not suppressed whole understory properties especially in richness, thus appeared to act as a filter selectively constraining the understory characteristics. However only long-term studies are essential for elucidating patterns and processes that cannot be inferred form short-term or space-for-time researches. Strong negative relationship between overstory and understory characteristics in conventional models surely reexamined.

• Applied Microscopy
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• v.29 no.1
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• pp.11-23
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• 1999

Ultrastructure of the ependymal cells of X-irradiated rats on their head were studied. Rats weighing $200\sim250gm$ were X-irradiated on their head and neck areas. Total exposures were 3,000 rads or 6,000 rads depending on experimental groups. And irradiated rats were sacrificed on 6 hours, 2 days and 6 days following the radiation exposures. Animals were perfused through the heart with 1% glutaraldehyde-1% paraformaldehyde solution, under ether-anesthesia. The tissues from the wall of lateral ventricles were fixed in the 2% osmium tetroxide solution. The results observed with electron microscope were as follow: 1. In 6 hours group, many ependymal cells were swelled, luminal portions of cytoplasms of some cells protruded into the ventricular lumen, and many cilia were lost or irregularly altered. 2. In 2 days group, ependymal cells were swelled more severely and subependymal edema were pronounced. 3. Protruded cytoplasm contained usually basal bodies of cilia, groups of mitochondria, endoplasmic reticula , etc. 4. Following X-irradiations, some protruded masses contained neural elements including the axon terminals with dense core vesicles. Axons and axon terminals were also found in the enlarged intercellular spaces among ependymal cells. From the above results, the heavy irradiation on the head area of the rat induced alteration of the ependymal cells lining the lateral ventricle. Hence the ependymal functions of selective barrier, protective barrier, and metabolic barrier could be altered following X-irradiation on the head.

• Economic and Environmental Geology
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• v.44 no.6
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• pp.503-511
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• 2011

The Upper Devonian Grosmont Formation in northern Alberta, Canada, underlies the erosion unconformity that formed between the Cretaceous and Upper Devonian. The formation is divided into four units on the basis of intercalated shales and showing a typical shelf environment of shallowing-upward. It was possible to separate four units(LG~UG3), considering the seismic interpretation attributes of polarity, continuity, frequency/spacing and amplitude and showing the reflection characteristics of the medium-high amplitude, medium-low frequency, good continuity, and subparallel reflection events. The formation can be interpreted as shelf or platform, based on in-situ core data. However, it is difficult, only with reflection attributes and features, to recognize the boundaries and sedimentary environment of parasequence. Therefore, we try to interprete by parasequence set in this study. The parasequence set was formed by erosion unconformity with systems tracts. The erosion unconformity can be recognized by facies data and karst, erosional surface. Grosmont carbonate deposits ranging from platform and shelf to shelf slope are; by wedge-shaped strata of characterized by complex sigmoid-oblique progradational configurations, reflecting a depositional history of upbuilding and outbuilding in response to sea-level changes. Most of the sedimentary units is interpreted as platforms under regression and lowstand environments that support is evidences. In particular, shale layer at the basal part of the highstand systems tracts represents the regressive to lowstand of sea level.

• Journal of Wetlands Research
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• v.20 no.3
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• pp.243-248
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• 2018

This study was carried out to clarify changes in community structure of Quercus acutissima dominant stand on the south slope of Mt. Danseok-san with fixed twenty quadrates. Five field surveys were conducted from Aug. 1999 to May 2018. During the period, the density lessened to 0.20 in 2012 comparing with 0.33 in 1999. However recent field study in 2018 showed moderate rising to 0.24. In 1999, the DBH class distribution of all trees formed reverse J curve. However, the reverse J curve was torn down, forming bell curve. In 2018, the curve showed similar shape of reverse J shape on the group of young trees, forming bell shape on the group of mature trees. It reveals that DBH 13cm is on the threshold of trees competition trend where the downtrend in the trees are on uptrend. The most dominant Q. acutissima formed bell curve. The peak of the curve shows the shift to the right of the graph as it gets lower by year.In case of Q. mongolica, shows a change in the shape of a low bell as the distribution curve increases. The oak stand in this study is in the process of changing from the initial stage of the secondary forest succession to the intermediate stage. The most dominant tree is Q. acutissima, and the sub-dominant tree is Q. mongolica in present. Considering the age distribution of the two competing tree species, the succession of this stand is expected to transfer to the Q. mongolica-dominant community.