• Journal of Microbiology and Biotechnology
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• 제27권2호
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• pp.335-341
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• 2017

The complexity of the bacterial recombination system is a barrier for the construction of bacterial mutants for the further functional investigation of specific genes. Several protocols have been developed to inactivate genes from the genus Pseudomonas. Those protocols are complicated and time-consuming and mostly do not enable easy construction of multiple knock-ins/outs. The current study describes a single and double crossover-recombination system using an optimized vector-free allele-exchange protocol for gene disruption and gene replacement in a single species of the family Pseudomonadaceae. The protocol is based on self-ligation (circularization) for the DNA cassette which has been obtained by overlapping polymerase chain reaction (Fusion-PCR), and carries an antibiotic resistance cassette flanked by homologous internal regions of the target locus. To establish the reproducibility of the approach, three different chromosomal genes (ncRNA31, rpoN, rpoS) were knocked-out from the root-associative bacterium Pseudomonas stutzeri A1501. The results showed that the P. stutzeri A1501 mutants, which are free of any plasmid backbone, could be obtained via a single or double crossover recombination. In order to optimize this protocol, three key factors that were found to have great effect on the efficiency of the homologous recombination were further investigated. Moreover, the modified protocol does not require further cloning steps, and it enables the construction of multiple gene knock-in/out mutants sequentially. This work provides a simple and rapid mutagenesis strategy for genome editing in P. stutzeri, which may also be applicable for other gram-negative bacteria.

• 교육시설
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• 제17권3호
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• pp.13-20
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• 2010

Seattle Public School Authority implements Inclusive Education which allows handicapped children to study in ordinary schools. This research is to analyze Inclusive Education system and find characteristics of school network and school planning. Survey was performed on 9 school districts and 54 public schools. The found results are following; 1) 54 schools adopt special education programs and legal barrier free design. All handicapped children groups are divided into level I through Ⅳ including mild level (level I,II) at all schools and multi-handicapped(severe level, level III, IV) at schools specified by school district or Seattle City. 2) Each school groups are transformed into self-contained classroom, therapy room and general room as a set in consideration of user communication and special education program. Also, existing classrooms are rearranged into small study spaces by using partition system. It allows ordinary schools to accomodate Inclusive Education through school network, classroom rearrangement and space partitioning.

• 한국멀티미디어학회:학술대회논문집
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• 한국멀티미디어학회 2004년도 춘계학술발표대회논문집
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• pp.533-537
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• 2004

고도 정보사회의 진전에 있어 정보의 수집, 정보의 전달 등을 위해, 여러 IT기기가 사용 되어지고 있다. 이러한 상황 속에서 IT기기를 장애자나 고령자를 포함, 누구나가 쉽게 이용 할 수 있도록 하는 유니버설디자인은 매우 중요한 일이다. 특히 상체부자유자의 인터페이스 장치로서 시선입력장치가 주목 받고 있다. 이러한 시대적인 흐름에서 본 연구에서는 Eye-gaze Interface를 이용한 시선입력만으로 컴퓨터상에서 한글입력이 가능한 한글 입력 시스템을 개발 제안한다. 또한 본 연구의 목적으로는 기존의 키보드, 마우스에 의한 입력장치와 Eye-gaze Interface를 이용한 입력 장치와의 정점과 단점을 비교 분석하고, 정보 사회에 있어서의 장애자와 고령자들의 Barrier Free에 관한 연구를 기본 목적으로 하고 있다 본 연구의 Eye-gaze Interface는 Eye Tech Digital System회사의 Quick Glance System를 사용한다 장애자와 고령자를 대상으로 한 사용자 평가 실험에 의해 실험을 진행 하였으며, 한글의 음운규칙에 관한 연구를 기초로 한글뿐만 아니라 영어, 일본어가 입력 가능한 입력 시스템을 제안한다. 이것은 컴퓨터 조작이 곤란한 장애자와 고령자가 일반인과의 자연스러운 커뮤니케이션이 가능하도록 한 것이다. 향후 Eye-gaze Interface를 이용한 응용분야 또한 게임, 영상, Eye Book, 자동차운전 둥 다양한 분야에 적용 가능하리라 기대된다.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2013년도 제44회 동계 정기학술대회 초록집
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• pp.552-552
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• 2013

In our study we consider Al2O3 ceramic substrates for Plasma Surface Treatments in order to improve deposited diamond surface and increase diamond deposition rate by applying DBD (Dielectric Barrier Dischrge) system. Because Plasma Surface Treatments was used as a modification method of material surface properties like surface free energy, wettability, and adhesion. By applying Plasma Surface Treatments diamond films are deposited on the Al2O3 ceramic substrates. DC Arc Plasmatron with mathane and hydrogen gases is used. Deposited diamond films are investigated by SEM (Scanning Electron Microscopy), AFM (Atomic Force Microscopy) and XRD (X-ray Diffractometer). Then the C-H stretching of synthetic diamond films by FTIR (Fourier Transform Infrared Spectroscopy) is studied. As a result, nanocrystalline diamond films were identified by using SEM and diamond properties in XRD peaks at (111, $43.8{\Box}$, (220, $75.3{\Box}$ and (311, $90.4{\Box}$ were shown. Absorption peaks in FTIR spectrum, caused by CHx sp3 bond stretching of CVD diamond films, were identified as well. Finally, we improved such parameters as depostion rate ($2.3{\mu}m$/h), diamond surface uniformity, and impurities level by applying Plasma Surface Treatments. These experimental results show the importance of Plasma Surface Treatments for diamond deposition by a plasma source.

• International Journal of Internet, Broadcasting and Communication
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• 제15권1호
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• pp.97-112
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• 2023

The purpose of this study is to design and test a new way of public transportation route guidance system for persons with disabilities, including wheelchair users. The guidance system is smartphone app-based, using, routes that involve disabled-friendly facilities in the vicinity can be searched. A database that contains publicly available data on low-floor bus services, location and extent of disabled-friendly facilities, and suitable subways and stations, was developed for this purpose. The app uses the database to access and query the required information. A pilot study was conducted to test the effectiveness of the guidance system. It was found that the system was able to convey information about the disabled-friendly routes and related guidance information even inside subway stations, effectively. The performance of the system was compared with route guidance services that do not explicitly use data on disabled-friendly services. A notable difference was observed in the travel time estimated by this program and other guidance services. The difference was around 4 to 15 minutes. This is significant savings for persons with disabilities if they use the app and service. The study thus shows that exclusive use of disabled-friendly data in route guidance will bring more benefits for persons with disabilities.

• 의료ㆍ복지 건축 : 한국의료복지건축학회 논문집
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• 제15권2호
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• pp.3-11
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• 2009

This study purports to investigate the status quo of the aged and disabled-friendly system in public health centers through the questionnaire survey and field interview. Data were collected from a total of 101 public health centers. The survey results revealed that the aged and disabled-friendly system in public health centers were not sufficient for meeting the needs of the elderly and disabled people. Based upon the study results, the following suggestions can be made to establish the aged and the disabled-friendly system in public health centers. The results of the study will make a contribution to establishing a good system for the aged and the disabled persons. In detail, they will be able to provide useful information for evaluating the status quo of the aged and the disabled-friendly system in many institutions. In addition, The results could be used as a practical strategy for improving the quality of the facilities for the aged and the disabled. In conclusion, the strategy and evaluation tool developed in the study will provide a good guide for establishing the aged and the disabled-friendly system in any institutions.

• 한국조경학회지
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• 제35권1호
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• pp.20-27
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• 2007

This paper presents a landscape design for the meeting plaza around the "Myeonmok" subway station. The site is located at 120-1 Myeonmok 1-dong, Jnngrang-gu, Seoul, and its area is approximately $2,664.7m^2$. The goal of the design was to make an environmentally friendly meeting and rest place which was related to the subway station. To achieve this goal, concepts of history, tradition, sense of place, community, environmental friendliness, and function were developed. For history, stone sculpture and art tiles symbolizing the paleolithic area were introduced because the site is located near an archaeological site of paleolithic min. For tradition, considering that the site is a 'sailing ship' form in terms of Pungsu theory, a sculpture symbolizing a sailing ship and paving patterns symbolizing waves were introduced. For asense of place, a grass hill, a waterfall and a pond symbolizing an old meadow for horse pasture was introduced. In addition, a multi-purpose round plaza as a meeting place for local community and subway users was proposed. A zelkova grove symbolizing a village forest was proposed for a restand relaxation area. All areas were designed to be environmentally friendly and barrier-free. Concepts for a defensible space wereadapted for safety because the site was a crime-prone area.

• Advances in materials Research
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• 제2권2호
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• pp.119-131
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• 2013

Impedance and electrical conduction studies of $Ba(Nd_{1/2}Nb_{1/2})O_3$ ceramic prepared using conventional high temperature solid-state reaction technique are presented. The crystal symmetry, space group and unit cell dimensions were estimated using Rietveld analysis. X-ray diffraction analysis indicated the formation of a single-phase cubic structure with space group $Pm\bar{3}m$. Energy dispersive X-ray analysis and scanning electron microscopy studies were carried to study the quality and purity of compound. The circuit model fittings were carried out using the impedance data to find the correlation between the response of real system and idealized model electrical circuit. Complex impedance analyses suggested the dielectric relaxation to be of non-Debye type and negative temperature coefficient of resistance character. The correlated barrier hopping model was employed to successfully explain the mechanism of charge transport in $Ba(Nd_{1/2}Nb_{1/2})O_3$. The ac conductivity data were used to evaluate the density of states at Fermi level, minimum hopping length and apparent activation energy.

• BMB Reports
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• 제46권1호
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• pp.17-24
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• 2013

Transcutaneous immunization refers to the topical application of antigens onto the epidermis. Transcutaneous immunization targeting the Langerhans cells of the skin has received much attention due to its safe, needle-free, and noninvasive antigen delivery. The skin has important immunological functions with unique roles for antigen-presenting cells such as epidermal Langerhans cells and dermal dendritic cells. In recent years, novel vaccine delivery strategies have continually been developed; however, transcutaneous immunization has not yet been fully exploited due to the penetration barrier represented by the stratum corneum, which inhibits the transport of antigens and adjuvants. Herein we review recent achievements in transcutaneous immunization, focusing on the various strategies for the enhancement of antigen delivery and vaccination efficacy.

• 의료ㆍ복지 건축 : 한국의료복지건축학회 논문집
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• 제13권4호
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• pp.37-46
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• 2007

The rehabilitation facilities for disabled people keeps going to grow with social changes that disabled people are dramatically increasing. This paper try to provide basic concept of the architectural planning for the disabled rehabilitation facilities. To accomplish this purpose this study has analyzed the behavior pattern of user with disabilities. This study has introduced the problem of using space in the rehabilitation and welfare facilities for the disabled and the architectural concept for resolve it. The architectural planning concept appears by barrier-free accessibility, safety for mobility and use, amenity integrated the spatial cognition and flexible structural system.