• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.40-40
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• 2017

Rice (Oryza sativa L.) is one of major crops of Vietnam. Rice plant contains many secondary metabolites that are potent to inhibit growth of other plants. This study was carried out to find potential plant growth inhibitors from rice plants. Seven rice varieties were cultivated in the fields affiliated to Hiroshima University, Japan; Mature stems and leaves from each variety were collected, and then they were extracted with methanol, hexane, ethyl acetate, butanol, and water. Total phenolic content and total flavonoid content were the highest in ethyl acetate extract. DPPH antioxidant assay results showed that the ethyl acetate extract also had higher $IC_{50}$ value. Therefore, the ethyl acetate extracts were selected for laboratory bioassay. Results showed that the two-local variety Re nuoc and Nan chon inhibited the germination of radish and barnyard grass seedlings at the greatest levels, as compared to other cultivars. HPLC quantification indicated that the ethyl acetate extracts of Re nuoc and Nan chon rice plant parts consists of phenolic and flavonoids compounds which are potent as plant growth inhibitors. Further laboratory bioassay and field experimentation will be conducted to validate the laboratory bioassay findings.

• Journal of Microbiology and Biotechnology
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• v.19 no.11
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• pp.1431-1438
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• 2009

The role of plant growth-promoting rhizobacteria (PGPR) in the phytoremediation of heavy-metal-contaminated soils is important in overcoming its limitations for field application. A plant growth-promoting rhizobacterium, Serratia sp. SY5, was isolated from the rhizoplane of barnyard grass (Echinochloa crus-galli) grown in petroleum and heavy-metal-contaminated soil. This isolate has shown capacities for indole acetic acid production and siderophores synthesis. Compared with a non-inoculated control, the radicular root growth of Zea mays seedlings inoculated with SY5 can be increased by 27- or 15.4-fold in the presence of 15 mg-Cd/l or 15 mg-Cu/l, respectively. The results from hydroponic cultures showed that inoculation of Serratia sp. SY5 had a favorable influence on the initial shoot growth and biomass of Zea mays under noncontaminated conditions. However, under Cd-contaminated conditions, the inoculation of SY5 significantly increased the root biomass of Zea mays. These results indicate that Serratia sp. SY5 can serve as a promising microbial inoculant for increased plant growth in heavy-metal-contaminated soils to improve the phytoremediation efficiency.

• Korean Journal of Environmental Agriculture
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• v.25 no.3
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• pp.268-275
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• 2006

ALA (5-aminolevulinic acid) has been proposed as a tetrapyrrole-dependent photodynamic herbicide by the action of the protoporphyrinogen IX oxidase (Protox IX). A study was conducted to determine photodynamic herbicidal effect of ALA on seedling growth of rice (Oryza sativa L.) and barnyard grass (Echinochloa crus-galli Beauv. var. oryzicola Ohwi) under dry and wet conditions. ALA effect on early plant growth of rice and barnyardgrass was greatly concentration dependant, suggesting that it promotes plant growth at very low concentration and inhibits at high concentration. No significant difference in herbicidal activity of biologically and synthetically produced ALAs on plant lengths of test plants was observed ALA exhibited significant photodynamic activity regardless of PSDIP and its duration. Significant shoot growth inhibition by ALA soaking treatment exhibited apparently, indicating that ALA absorbed through root system was translocated into shoot part of plants. ALA reduced plant heights of rice and barnyardgrass seedlings by 6% and 27%, respectively, showing more tolerant to ALA in rice under wet condition. Leaf thickness was reduced markedly by ALA with increasing of ALA concentration, due to mainly membrane destruction and severe loss of turgidity in mesophyll cells, although the epidermal was little affected. It was observed that photodynamic herbicidal activity of ALA applied by pre-and post-emergence application exhibited differently on plant species, and that the activity of ALA against susceptible plants was highly correlated with growing condition.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.5
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• pp.402-406
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• 2003

Greenhouse and laboratory studies were conducted to determine the allelopathic potentials of extracts or residues from sweet potato (Ipomoea batatas L. (Lam). The extracts applied on filter paper in a Petri dish bioassay significantly inhibited root growth of alfalfa. Aqueous leachates at 40g dry tissue $\textrm{L}^{-1}$ (g $\textrm{L}^{-1}$) from leaves showed the highest inhibition against alfalfa, and followed by stems and roots. Alfalfa root growth was significantly inhibited by methanol extracts of the same plants as the concentration increased. The effect of residue incorporation into soil on seedling growth of com, soybean, barnyard grass and eclipta was examined in the greenhouse, and results showed that the leaf residues at 200g $\textrm{kg}^{-1}$ by plant parts inhibited shoot dry and root dry weights of test plants by 60-80%. By means of HPLC, causative allelopathic substances present in plant parts of sweet potato "Sinyulmi" were identified as coumarin, trans-cinnamic acid, o-coumaric acid, p-coumaric acid, and chlorogenic acid. Total content of these compounds for leaves extracts were detected as the greatest amount in EtOAc fraction, especially trans-cinnamic acid was the greatest component. These results suggest that sweet potato plants have herbicidal potentials, and that their activities exhibit differently depending on plant parts.ant parts.

• Plant Resources
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• v.7 no.1
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• pp.69-76
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• 2004

Lettuce (Lactuca sativa L.) is known to contain water-soluble substances that are biologically active. Aqueous or methanol extracts and residues from leaves of lettuce plants were assayed to determine their allelopathic effects, and the causative allelochemicals from fractions were quantified by means of HPLC analysis and bioassayed. Extracts from oven-dried leaf samples were more phytotoxic than those from freeze-dried samples. Leaf extracts of 40 g L$^{-1}$ were completely inhibitory on root growth of alfalfa (Medicago sativa L.), while root growths of barley (Hordeum vulgare L.) and soybean (Glycine max L.) were less sensitive. Early seedling growth of both alfalfa and barnyard grass (Echinochloa crus-galli) was significantly reduced by methanol leaf extracts. The major allelopathic substances analyzed by HPLC were coumarin, trans-cinnamic acid, o-coumaric acid, p-coumaric acid and chlorogenic acid. Of them p-coumaric acid was found as the greatest amount (8.9 mg 100 g$^{-1}$ ) in the EtOAc fraction; only coumarin was found in all the fractions. Hexane and EtOAc fractions of L. sativa reduced alfalfa root growth more than did BuOH and water fractions. These results suggest that lettuce had potent herbicidal activity, and that its activity differed depending on type and amount of causative compounds by fraction.

• Journal of Korea Water Resources Association
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• v.47 no.10
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• pp.973-983
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• 2014

This study focused on the selection of appropriate plant species of VFS (vegetative fiter strips) and the assessment of VFS effects for reducing NPS (non-point source) pollution from uplands. The experimental field was constructed with 1 control and 6 treated plots in the upland area of $1,500m^2$ with 5% slope which is located in Gunwi-gun, Gyeongbuk province. Six vegetation including Chufa, Common crabgrass, Barnyard grass, Turf grass, Tall fescue, Kenturky bluegrass, were applied to install VFS systems during the study period from June 2011 to Dec. 2012. The results of this study showed that 6.1~77.8% in runoff and 15.6~90.3% in TS, 49.9~96.6% in T-P, and 6.7~91.1% in T-N were reduced from the VFS treated plots. Generally high reduction effects were observed from TS, T-P, T-N, and SS, while BOD, TOC, and $NO_3^-$ showed low reductions. The best vegetation type was Turf grass showing higher reduction effects of NPS pollutions and having relatively easier maintenance efforts compared to other vegetations selected in this study. Based on these results, VFS technique found to be an effective management practice for reducing agricultural NPS pollutions in Korean upland conditions. Further study needs to be performed through various field experiments with long term monitoring in order to develop a design manual of VFS system for practical applications.

• Applied Biological Chemistry
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• v.36 no.5
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• pp.381-386
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• 1993

New twenty five Imazethapyr derivatives, [2-(4-isopropyl-4-methyl-5-oxo-2-imidazolin -2-yl)-3-(N-methyl-N-X(sub.)-phenylaminooxoacetyl)-5-methylpyridine] were synthesized. and The quantitative structure activity relationships (QSARs) between their post-emergence herbicidal activity$(pI_{50})$ values in vivo against Barnyard grass (Echinochloa crus-galli) and physicochemical parameters of substituents(X) of 3-(N-methyl-N-X(sub.)-phenylaminooxoacetyl) group have been studied. From the basis on the findings, in case of post-emergence, the activities were dependent on the steric constant$(E_s<0)$ and electron donating $(\sigma<0)$ effect by subsitituents(X) of 3-(N-methyl-N-X(sub.)phenylaminooxoacetyl) group. Therefore, The most effective compound,15 (4-t-butyl group) and 20 (3,5-dimethyl group) were examined in this study. And the conditions on the compounds predicted to show higher herbicidal activity were also discussed.

• Applied Biological Chemistry
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• v.50 no.4
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• pp.316-320
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• 2007

Three phytotoxic compounds were isolated from the culture broth of Chaetomium sp. through silica gel column chromatography and HPLC (RP-18). Their chemical structures were elucidated as chaetoglobosin F, chaetoglobosin C and chaetoglobosin E on the basis of instrumental analyses such as $^1H-NMR,\;^{13}C-NMR$, and HMQC. They inhibited the root growth of barnyard grass with the $IC_{50}$ values of 66, 65, and $67{\mu}g/ml$, respectively.

• The Korean Journal of Pesticide Science
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• v.8 no.2
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• pp.145-149
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• 2004

A series of 2-[4-(6-chloro-2-benzoxazolyloxy)phenoxy]-propionamide were synthesized and evaluated for post herbicidal activity under upland condition. 2-[4-(6-chloro-2-benzoxazolyloxy)phenoxy]-N-(2-fluorophenyl)-N methyl propionamide showed high herbicidal activity against barnyardgrass with good selectivity on rice.

• Applied Biological Chemistry
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• v.38 no.1
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• pp.90-94
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• 1995

Post emergence herbicidal activities$(pI_{50})$ of X-substituted phenylvinylsulfone derivatives(S) in-vivo against rice(Oryza sativa L.), Barnyard grass(Echinochloa crus-galli) and Pickerelweed(Monochoria vaginalis Presl) were measured by the pot test under paddy conditions. The (S) showed herbicidal symptom rapidly with lower activity(average $pI_{50}=2.0$) as proherbicide, which was excellent tolerance to rice. The structure activity relationships(SAR) were analyzed using such a physicochemical parameters as hydrophobic$({\pi})$ and molecular orbital(MO) quantity by the multiple regression technique, and discussed with quantum pharmacology. The herbicidal activities were related to the hydrophobic$({\pi})$ effect of X-substituent and orbital(HOMO & LUMO) energy. In case of Pickerelweed, the effect was rationalized by parabolic function of ${\pi}$ constant, where the optimal value of ${\pi}$ was 1.10. An increase in hydrophobicity and negative orbital energy by the electron attracting X-substituent may contribute to the herbicidal activity. Based on results proposed from SAR analysis, the mode of herbicidal action could be assumed.