• 한국환경과학회지
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• 제11권10호
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• pp.1023-1030
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• 2002

The effects on photosynthesis of NaCl(0, 0.2, 0.4, 0.6, 0.8 or 1.0 M) were examined in etiolated barley seedlings. Chlorophyll(Chl) a, Chl b and carotenoid contents, Chl a fluorescence and quenching coefficients of Chl fluorescence have been determined in the primary leaves of etiolated barley seedlings cultivated under low light(60 $\mu$㏖ $m^{-2}\;s^{-1}$). Chl a, b, and carotenoid contents were decreased remarkably in comparison with the control at 0.4 M NaCl. However, the value of Fo and Fv were decreased at 0.6 M NaCl and the ratio of Fv/Fm were deceased at 1.0 M NaCl. Chlorophyll synthesis was seriously inhibited from 0.4 M NaCl, and the photosynthetic electron transport system was inhibited from 0.6 M NaCl. Quantum of photosystem II reaction center was inhibited at 1.0 M NaCl. The effects of NaCl on the Chl content were raised in a 6 hrs, but the effects of NaCl on the value of Fo, Fv and Fv/Fm were raised in 30 hrs. The value of qP was decreased in comparison with the control at all concentrations, but there was a small change in the value qE. These results provide evidence that NaCl inhibited effects of various concentration of NaCl were inhibited quinone redox, however, proton gradient between thylakoid membranes was little damaged.

• 한국작물학회지
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• 제40권2호
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• pp.195-202
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• 1995

토성과 지하수위를 달리하여 맥류의 생육과 수량에 미치는 반응을 알아보고자 올보리와 그루 밀을 공시하여 1992년 10월∼1993년 6월에 걸쳐 550l의 대형 포트 시험을 수행하였다. 토성을 식양토와 사양토로 조성하여 전 생육기에 거쳐 지하수위가 20cm, 30cm, 40cm, 50cm, 70cm가 되도록 조절하고 비가림 재배를 실시한 결과를 다음과 같다. 1. 간장과 지상부건물중은 지하수위가 높아짐에 따라 감소하였으며 그 정도는 밀보다 보리에서 더욱 크게 나타났고 지하부중에 대한 지상부중 비율은 지하수위가 높아짐에 따라 증대하였는데 그 정도는 보리에서 더욱 뚜렷하였으며 지하수위의 상승은 지상부보다 지하부의 생장에 더욱 크게 영향하였다. 2. 생육후반기(93년 5월 18일)의 신선엽수와 신선엽면적는 보리와 밀 모두 지하수위 30∼40cm 이상에서 감소하였고 가장 많은 신선엽수와 신선엽면적은 사양토는 지하수위 50cm, 식양토는 지하 수위 70cm에서 나타났다. 3. 엽록소 함량은 지하수위가 높아짐에 따라 감소되었는데 보리가 밀보다 토성에 따른 영향이 크게 나타났으며 광합성 능력은 지하수위 20cm에서 뚜렷이 저하하였다. 4. 출수기는 지하수위가 높아지면서 촉진되어 지하수위 20cm의 경우에 사양토에서 보리의 경우 2∼3일, 밀의 경우 4일 정도 빨라졌고 식양토에서는 별로 차이가 없었으며 등숙기는 지하수위가 높아짐에 따라 촉진되어 지하수위 20cm에서 보리의 경우 5∼7일, 밀의 경우 10일 정도 빨라졌다. 5. 수량은 토성에 따라 지하수위 50∼70cm에서 가장 많았으며 지하수위가 높아짐에 따라 감소 되어 지하수위 20cm에서 가장 크게 떨어졌는데 그 감소 정도는 보리에서 71.1％(식양토)∼72.2％(사양토)였고 밀에서 41.0％(식양토)∼60.0％(사양토)이었다. 6. 수량구성요소 면에서 단위면적당 이삭수는 지하수위가 높아짐에 따라 보리와 밀 모두 줄었으나 이삭당립중은 보리에서는 증가했고 밀에서는 감소하였다. 7. 수량과 엽록소 함량간에 보리와 밀 모두 높은 상관관계를 나타내었으며 보리에서는 신선엽면적이, 밀에서는 지상부건물중, 지하부에 대한 지상부중량 비율이 수량과 높은 상관을 보였다. 이상의 결과로 보아 토성에 따라 지하수위 50∼70cm에서 맥류의 생육과 수량은 최대가 되며 비교적 안전한 지하수위는 사양토에서는 30cm, 식양토에서는 40cm라 하겠으며 지하수위 상승에 따른 수량감소비율은 보리에 비하여 밀에서 더욱 크게 나타났다.

• 한국식품영양과학회지
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• 제37권12호
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• pp.1627-1631
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• 2008

다양한 방법으로 건조한 보리 잎의 생리활성을 분석한 결과, 보리 잎의 추출수율은 건조방법 간에 유의적인 차이를 보이지 않았다. 총 페놀함량은 건조방법별 함량에 유의적인 차이가 없었으나, 총 플라보노이드 함량은 열처리 후 건조한 보리 잎에서 생잎 다음으로 높게 나타났고, 음건, 진공 동결 건조, 전자레인지 후 건조한 보리 잎에서는 유의성을 보이지 않았다. SOD 유사활성은 생잎 $95.69{\pm}0.33%$, 열처리 후 건조한 보리 잎 $94.96{\pm}0.77%$, 음건 $92.04{\pm}1.35%$, 진공 동결건조 $91.53{\pm}0.40%$, 전자레인지 건조 $92.58{\pm}0.27%$로 90% 이상의 높은 활성을 보였으며, 대조군인 항산화제 sesamol $88.42{\pm}0.80%$, tocopherol $88.86{\pm}0.61%$, BHT $86.62{\pm}0.89%$에 비해 유의적으로 높은 활성을 나타냈다. 또한 생잎과 전자레인지 건조한 보리 잎을 제외하고 80% 이상의 peroxyl radical 소거능 및 90% 이상의 높은 xanthine oxidase 저해능을 보였다. 본 연구결과, 건조방법별 보리 잎의 총 페놀, 총 플라보노이드 함량은 건조방법에 따라 차이를 보였으나, 항산화 능력에는 크게 영향을 미치지 않는 것으로 사료된다.

• The Plant Pathology Journal
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• 제22권4호
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• pp.348-352
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• 2006

Barley mild mosaic virus(BaMMV) is a soilborne Bymovirus vectored by root-infecting fungus, Polymyxa graminis. Mechanism of cultivar's resistance to BaMMV in field tests are difficult to assess since resistance could be either due to the virus or to P. graminis, or both. Whereas, available mechanical inoculation methods for BaMMV and other related viruses are labor intensive, give inconsistent results and generally result in low infection rates. Inoculation method using stick with gauze(SWG) was developed for BaMMV. The improved method proved to be simple, efficient, and reliable. The infected leaf tissues were preserved by drying in a frozen state under high vaccum(freeze dried barley infected leaves) to circumvent reduction of virus infectivity during storage. Five Korean barley cultivars were mechanically inoculated with BaMMV-infected sap by the improved method. Infection rates obtained were compared with natural infection. Cultivar Naehanssalbori showed resistance to BaMMV in the field trials but was found highly susceptible in the greenhouse tests by mechanical inoculation, indicating that the field resistance may be possibly due to resistance to P. graminis.

• The Plant Pathology Journal
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• 제34권3호
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• pp.236-240
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• 2018

Crop yield is critically related to the physiological responses and disease resistance of the crop, which could be strongly affected by high temperature conditions. We observed the changes in the growth of barley under higher than ambient air-temperature conditions using a temperature gradient field chamber (TGFC) during winter and spring. Before the stem extension stage of barley growth, Cladosporium sp. spontaneously appeared in the TGFC. The severity of disease became serious under warmer temperature conditions. Further, the stomata closed as the severity of the disease increased; however, stomatal conductance at the initial stage of disease was higher than that of the normal leaves. This was likely due to the Iwanov effect, which explains that stressed plants rapidly and transiently open their stomata before longer-term closure. In this study, we tested three optical methods: soil-plant analysis development (SPAD) chlorophyll index, photochemical reflectance index (PRI), and maximum quantum yield (Fv/Fm). These rapid evaluation methods have not been used in studies focusing on disease stress, although some studies have used these methods to monitor other stresses. These three indicative parameters revealed that diseased barley exhibited lower values of these parameters than normal, and with the increase in disease severity, these values declined further. Our results will be useful in efficient monitoring and evaluation of crop diseases under future warming conditions.

• 한국환경농학회지
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• 제40권4호
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• pp.345-352
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• 2021

BACKGROUND: Salinity is one of the major limiting factors in agriculture that affect the growth and productivity of crops. It is economically difficult to artificially purify the soil affected by salt. Therefore, the use of plant growth-promoting bacteria (PGPB) in an effort to reduce stress caused by salt is emerging as a cost-effective and environment-friendly method. In this study, the purpose was to isolate the salt-tolerant bacteria from the rhizosphere soil and identify their ability to promote plant growth under salt stress condition. METHODS AND RESULTS: The isolates KST-1, KST-2, AST-3, and AST-4 that showed plant growth-promoting activity for barley in salt conditions were close to Bacillus cereus (KST-1, KST-2, and AST-4) and Bacillus thuringiensis (AST-3) and showed high salt tolerance up to 7% of additional NaCl to the media. When inoculated to barley, the strains had only minor effect on the length of the barley. However, the concentrations of chlorophyll in the barley leaves were found to be higher from the bacteria-inoculated pots than those from the uninoculated control. In particular, the chlorophyll concentration in Bacillus cereus AST-4 experiment was 5.45 times higher than that of the uninoculated control under the same experimental condition. CONCLUSION(S): The isolated salt-tolerant bacteria were found to influence on chlorophyll concentration of the barley. As represented by the strain AST-4, microbes may suggest a cost-effective and environmentally benign method to alleviate salt stress of crops cultivated in salt-accumulated soils such as reclaimed lands.

• 한국작물학회지
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• 제30권3호
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• pp.314-319
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• 1985

장일과 단일조건에서 보리 7개 품종의 엽수, 유수발육, 유수장, 영화수 및 지엽전개일수의 차이를 검토하고자 실험한 결과를 요약하면 다음과 같다. 1. 장일조건에서 품종간 주간엽수의 차이는 거의 없었으나 단일조건에서는 조숙종은 6∼7엽, 만숙종은 9∼10 엽이었다. 2. 단일조건에서 조숙인 조강보리, 오월보리는 유수의 발육이 이식 24일경에 X기에, 만숙인 CI 15446은 32일경에 X기에 도달하였다. 3. 유수장의 변화는 장일보다 단일조건에서 커서 조숙종은 이식 30일에 만열종은 51일경에 최대 신장을 보였다. 4. 단일에서 일수입수가 2배정도 많았으며 감광성이 적은 품종은 조강보리, 오월보리 등이었고 큰 품종은 새올보리 ＞CI 15446 ＞항미 순위이었다. 5. 본 실험결과로 조숙종은 만숙종에 비해 단일조건에서 주간엽수의 변이가 적고, 주간의 유수발육 및 유수장신장의 지연, 출수기의 지연이 적었다.

• The Plant Pathology Journal
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• 제36권5호
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• pp.497-502
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• 2020

Barley yellow dwarf virus (BYDV) is an economically important plant pathogen that causes stunted growth, delayed heading, leaf yellowing, and purple leaf tip, thereby reducing the yields of cereal crops worldwide. In the present study, a reverse transcription recombinase polymerase amplification (RT-RPA) assay was developed for the detection of BYDV in oat leaf samples. The RT-RPA assay involved incubation at an isothermal temperature (42℃) and could be performed rapidly in 5 min. In addition, no cross-reactivity was observed to occur with other cereal-infecting viruses, and the method was 100 times more sensitive than conventional reverse transcription polymerase chain reaction. Furthermore, the assay was validated for the detection of BYDV in both field-collected oat leaves and viruliferous aphids. Thus, the RT-RPA assay developed in the present study represents a simple, rapid, sensitive, and reliable method for detecting BYDV in oats.

• 한국작물학회지
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• 제48권5호
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• pp.407-412
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• 2003

In order to measure an antifreezing tolerance, antifreeze proteins accumulated upon cold acclimation in apoplast were analyzed. As Dongborilho were cold-acclimated for 3 to 74 days there was an abrupt increase in apoplastic proteins up to 30 days and then decrease to the similar levels. Among the known antifreeze proteins, CLP produced in E. coli. and TLP purified from apoplast were used to generate antisera that allow to measure and localize the proteins in leaves of barley. The CLP of 27.7 kDa and TLPs of 6, 26, 27, 30, and 31 kDa were increased in their amounts in apoplast as cold treatment being longer. There was a correlation among the amounts of those proteins accumulated in apoplast and freezing tolerance as shown in field and ion leakage tests for five cultivars. The deposit of CLP was localized in the marginal area and the area adjacent to leaf vescular bundle cells in an increasing manner according to duration of cold acclimation but no variation was observed in terms of it's distribution. Based on the close correlation between levels of antifreeze proteins and degrees of freezing tolerance, the immunological methods was to develop to estimate a freezing tolerance in barley.

• 한국환경과학회지
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• 제6권4호
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• pp.369-378
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• 1997

The effects of various intensity of W-B on barley seeding were investigated by PS I and II activities and chlorophyll fluorescence. The Inhibitory effect of UV-B radiation on electron transport activity was Increased as the intensity of UV-B Irradiation was increased. Especially, PS I is more sensitive to UV-B radiation than PS I is. By the addition of uncle electron donor, DPC, to the chloroplasts of the barley seedlings treated with UV-B, the photoreduction of DCPIP was recovered by only 1 IBI on electron transport activity. However, the activity of PS II was Inhibited by 45% by the treatment with UV-B, but recovered it only 11% by the addition of DPC. These suggest that other sites besides the oxidation site of PS II may be affected more by UV-B Irradiation. As the intensify of UV-B was Increased, Fo was Increased while Fv was decreased, and thus Fv/Fm was decreased. This means that photochemical efficiency was reduced. With this parameters, it might be that UV-B radiation affected adversely to around PS II.