• Journal of Mushroom
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• v.16 no.4
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• pp.293-298
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• 2018

'Geumhyang2ho', a new brown and labor-saving variety of Flammulina velutipes, was bred by spontaneous crossing between 'Geumhyang' and 'Garlmoe' during demonstration cultivation in bag culture farm of oyster mushroom in 2013. During bottle cultivation, the temperature was maintained at $16^{\circ}C$; the variety displayed good quality compared to the control ('Geumhyang'), without necessitating vinyl cone treatment. The time periods necessary for mycelial growth, fruit body growth, and primordia formation were 24 days, 11 days, and 8 days, respectively. The total cultivation period was 43 days, which was 2 days longer than that of the control. The pileus diameter was smaller, but the pileus thickness and stipe diameter were greater than those of the control. The pileus color was yellowish ivory, which was very similar to that of the control. The yield of 'Geumhyang2ho' was 118 g per 850 ml bottle, which was markedly lower than that for bottle cultivation with vinyl cone treatment. However, its good quality suggests the potential of the labor-saving bottle-based cultivation of 'Geumhyang2ho' without vinyl cone treatment in oyster mushroom farms.

• Journal of Mushroom
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• v.21 no.4
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• pp.261-265
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• 2023

We aimed to develop outstanding domestic varieties suitable for both columnar and cylindrical-shape substrates, aiming to replace foreign varieties. and bred a high-quality new strain named 'Charmjon', using genetic resources collected from Japan and China. The optimal cultivation temperature for Charmjon's mycelial growth was found to be 25℃, and its mycelial growth at 15℃ and 25℃ was superior to the control variety. In terms of mycelial growth characteristics based on the substrate, Charmjon exhibited excellent mycelial strength on PDA medium compared to the control variety. Through columnar and cylindrical-shape substrates cultivation, we assessed the quantity and morphological characteristics of the fruiting bodies. The results confirmed that Charmjon can be produced stably using both cultivation methods, and it showed higher yields and individual weights than the control variety. In addition, the color of the pileus was notably darker, and the shape of the pileus varied depending on the cultivation method. The test of genetic diversity revealed that Charmjon has distinct genetic characteristics compared to the control varieties.

• Korean Journal of Medicinal Crop Science
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• v.20 no.5
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• pp.365-371
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• 2012

This study was carried out to know the characteristics of flowering and bearing fruit, the optimum period, regions and methods for seed harvesting, the optimum temperatures for seed storage and germination, and the optimum period for sowing at nursery bed and seedling transplanting of Valeriana fauriei Briquet. The flowering and bearing fruit of Valeriana fauriei was developed from the before-year root. Optimum period for seed harvest of Valeriana fauriei was from late July to middle August, and optimum areas were the high elevated areas over 500 m above the sea level as Jinbu-myeon, Pyeongchang-gun, Gangwon-do. Using of net-bag for seed harvesting was the effective method to gather the full ripe seed, and bagging of net-bag was necessary from the season of middle May that was the flowering middle-stage. Germination rates don't show the difference among the different temperatures of storage as approximately 41% at $-20^{\circ}C$, $2^{\circ}C$ or $20^{\circ}C$ of seed storage temperatures. The optimum temperature range was in $15{\sim}30^{\circ}C$ for seed germination at nursery bed. The optimum period for seed sowing at nursery bed was the late February, and the optimum period for seedling transplanting was the middle April.

• Journal of Drive and Control
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• v.20 no.3
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• pp.35-41
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• 2023

Radish and Chinese cabbage are the most produced and consumed vegetables in Korea. The mechanization of harvesting operations is necessary to minimize the need for manual labor. This study to develop and evaluate the performance of a multi-purpose driving platform that can apply modular Radish and Chinese cabbage harvesting devices. The multi-purpose driving platform consisted of driving, device control, engine, hydraulic, harvesting, conveying, and loading part. Radish and Chinese cabbage harvesting conducted using the multi-purpose driving platform each harvesting module. The performance of the multi-purpose driving platform was evaluated the field efficiency and loss rate. The total Radish harvesting operation time 34.3 min., including 28.8 min., of harvesting time, 1.9 min., of turning time, and 3.6 min., of replacement time of bulk bag. During Radish harvesting, the field efficiency and average loss rate of the multi-purpose driving platform were 2.0 hr/10a and 3.1 %. Chinese cabbage harvesting operation 49.3 min., including 26.6 min., of harvesting time, 4.6 min., of turning time, and 18.1 min., of replacement time of bulk bag. During Chinese cabbage harvesting, the field efficiency and average loss rate of the multi-purpose driving platform 2.1 hr/10a and 0.1 %. Performance evaluation of the multi-purpose driving platform that harvesting work was possible by installing Radish and Chinese cabbage harvest modules. Performance analysis through harvest performance evaluation in various Radish and Chinese cabbage cultivation environments is necessary.

• The Korean Journal of Mycology
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• v.31 no.3
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• pp.192-199
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• 2003

This study was carried out to investigate the artificial cultivation of Pleurotus eryngii on the optimal medium vessel, periods of cultivation and the optimal method of pinheading for both yield and quality of fruiting body were also performed. The optimal composition of sawdust medium in polypropylene(PP) bottle was combination of sawdust(70%) and corncob(30%) but increased amount of corncob delayed the period of mycelial growth. The mycelial growth and the yield of fruiting body in the medium with beat pulp were worse than that without beat pulp. The optimal composition of nutrients for both yield and quality of fruit body tuned out to be a combination of rice bran(12%), wheat bran(12%) and cottonseed cake(6%). Additions of zeolite, shell lime and bean curd dregs were not effective in mycelial growth and yield of fruit body. When testing size of PP bottle for cultivation, the larger of bottle mouth is, the more pinheading number found, but the number of available fruit body is not significantly different. The culture in $1100\;ml-{\phi}75\;mm$ bottle is the best in the yield and quality of fruit body than those in $555\;ml-{\phi}50\;mm,\;850\;ml-{\phi}58\;mm,\;850\;ml-{\phi}65\;mm\;and\;1100\;ml-{\phi}65\;mm$ bottle. Using the PP bag for cultivation, a square shaped bag was better than a round shaped and black square shaped in mycelial growth and yield of fruit body. The most suitable period of incubation was 35 days after inoculation at $22{\pm}2^{\circ}C$. When the incubation periods was decreased less than 35 days, the pileus formation and yields were very bad but a pinheading condition looked similar, For an optimal pinheading, turning upside down was better than standing and covering.

• Journal of Mushroom
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• v.14 no.4
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• pp.207-210
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• 2016

In 2010, a new variety for sawdust cultivation,was produced by monokaryotic-monokaryotic crossing between Sanjo 701ho and FMRI0995. The optimum temperature for Sanjo 708ho mycelial growth was $25^{\circ}C$; mycelial growth on potato dextrose agar was 51.6 mm over 7 days. The fruiting pattern was sporadic, and the optimal temperature range for fruiting was $5{\sim}20^{\circ}C$. Regarding fruiting body characteristics, the pileus was 65.8 mm in size, 16.1 mm in thickness, and hemispherical in shape. The stipe was 42.6 mm in and 19.6 mm in thickness. Mproductivity was good during thelow-temperature period from autumn to spring, with an average productivity of 310.7 g/bag.

• Journal of Mushroom
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• v.8 no.1
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• pp.16-21
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• 2010

Sparassis crispa(Cauliflower mushroom) was an edible mushroom that shows remarkably high contents of 1.3-${\beta}$-Dglucan compared to other edible mushroom. The mushroom was known to give high antitumor and immunology activated, and then this mushroom was recently cultivated in Japan and Korea. However, cultivation methods ware becoming kept in secret or patents by some companies with complicated procedures. And it was not established cultivation methods of Sparassis crispa up to now. This study was conducted to solve the problem by short-log cultivation method of Sparassis crispa. Some factors effecting on the mycelial growth and primordial formation of Sparassis crispa were investigated. We could produce the mushroom using short-woods of Larix leptolepis, Pinus densiflora, Pinus rigida and Quercus acutissima. We get to high yield fruit-body on short-log cultivaiton of Pinus rigida. And soaking for 8hours in water solution containing 5% uncooked yeast with short-wood of Pinus rigida. The optimal moisture content and temperature were 90~95% and $23{\sim}25^{\circ}C$, respectively.

• Journal of Mushroom
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• v.9 no.2
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• pp.80-83
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• 2011

Sparassis crispa(Cauliflower mushroom) was an edible mushroom that shows remarkably high contents of ${\beta}$-glucan compared to other edible mushroom. In this study, we aimed to select suitable conditions and materials for mycelial growth and fruit body production. The longer saccharification time of barely result in higher sugar content for eight hours. The optimal sugar content of media for mycelial growth was showed at level of 6 $^{\circ}Brix$. Optimum temperature and pH for mycelial growth in liquid spawn were $25^{\circ}C$ and pH 5.0~6.0, respectively. In case of sawdust was used Larix kaempferi as main material, the fruit body yield and cultivation period of supplemented with 10% wheat flour and 20% corn flour were highest and fastest, respectively.

• Journal of Mushroom
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• v.7 no.3
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• pp.110-114
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• 2009

Sawdust bag cultivation of Shiitake mushroom (Lentinula edodes) is getting increase. The mycelia browning on the substrate surface is important for the stable production. The development of methods for the rapid mycelia browning is quite required. In this study changes in intracellular enzyme during the mycelial browning were investigated to find the rapid mycelia browning. Mycelia of L. edodes was changed into brown color while it grew in agar and liquid media like sawdust substrates. Mycelia of L. edodes was started to change color at 25 days after inoculation and browning was occurred in whole mycelia colony at 30 days and browning was completed at 40 days. The activities of enzymes was evaluated in these periodically color changing mycelia. Laccase activity was highest at 15 days after inoculation on PDB, but it gradually decreased from 15 days. Tyrosinase activity drastically increased in period between 30 days and 40 days while mycelia browning was progressed. The kinds of phosphotase identified by electrophoresis were esterase, acid phosphotase, and alkaline phosphotase. Activities of phosphotase were increased before the initiation of mycelial browning but they were decreased after browning.

• Journal of the Korean Society of Tobacco Science
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• v.18 no.2
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• pp.126-131
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• 1996

In this study effects of isolation distance, transplanting time of maternal plants, and bagging of flower head with the gauze-cloth bag on the outcrossing of burley tobacco (Nicotiana tabacum L.) were investigated. Also the effect of fertilizer level and control of the number of capsules per plant on seed production and quality were examined. A male sterile line. produced 0.3 to 3.8 capsules Per plant when it was Planted with normally flowering tobacco with the average outcrossing of 7.2 plants, ranging from 2 to 18 out of 20 plants. With the farther the isolation distance between maternal plants and pollen donor plant, the lower the outcrossing occurred. Outcrossing occurred even at the isolation distance of 312 m. When the maternal plants were transplanted 35 days after transplanting the pollen donor ones, the outcrossed plants were not decreased significantly. The bagging of the flower head with the gauze-cloth bas (#0.9∼ 1.0 mm) decreased the outcrossed plants significantly, but couldn't prevent the outcrossing completely. The seed amount per plant was higher in the highly fertilized cultivation. The number of seed capsules per plant affected significantly on seed yield and quality. When the seed capsules was controlled by 30 or 50 capsules per plant, the weight of 1,000 seeds and germination rate were higher than those with 70 or 90 capsules per plant. Key words : Nicotiana tabacum, outcrossing, bagging.