• Journal of Photoscience
• /
• 제9권2호
• /
• pp.370-372
• /
• 2002

In order to investigate effect of the carotenoids (Car) on aggregation of Bacterochlorophyll (BChl) in chlorosome, we studied the spectral difference in aggregates of BChl e formed in the absence and presence of a few kinds of Car in dimethyl sulfoxide (DMSO) -water solution. The absorption spectra of aggregates made of only BChl e and those made of a mixture of BChl e and Car were almost the same. However, the kinetics and circular dichroism (CD) spectra of aggregate of these were markedly different by kind of Car. Specifically, the rate of aggregation for a mixture of BChl e and isorenietene that contains phenyl as end groupe was faster than that for only BChl e. CD spectra of aggregates made of a mixture of BChl e and isorenietene dramatically changed compared to that made of only BChl e. We propose that BChl might form several kinds of rod-like supramolecular structures to in the presence of some kind of Car in chlorosome.

• 농업과학연구
• /
• 제19권1호
• /
• pp.28-32
• /
• 1992

태국의 토양으로부터 분리한 젤라틴 분해능을 갖는 광합성세균 T-20주(株)는 직경이 $0.6{\sim}0.7{\mu}m$의 적색 간균으로써, Bacteriochlorophyll a의 흡수극대는 870nm이었고 또한, 퀴논종(種)은 UQ-8을 나타내었다. 유기 화합물로써 citrate, aspirate, glutamate, fractose 등을 이용하였다. 이상의 결과로부터 T-20주는 Bergey's Manual of Systematic Bacteriology(1989)에 기재된 Rhodocyclus gelatinosus와 일치한다.

• 한국식물학회:학술대회논문집
• /
• 한국식물학회 1999년도 Proceedings of the 17th Symposium on Plant Biology Environmental Stress and Photosynthesis
• /
• pp.55-67
• /
• 1999

A puc -deleted cell of Rhodobacter sphaeroides grows with a doubling time longer than 160 h under the light-limiting photoheterotrophic ( 3 Watts [W]/㎡) conditions due to an absence of the peripheral light-harvesting B800-850 complex. A spontaneous fast-growing mutant, R.sphaeroides SK101 was ioslate dto have∼40-h doubling at 3 Watts/㎡, while the growth of the mutant was not distinguished from its parental strain during both aerobic and light-saturating photoheterotrphic (10W/㎡) growth. The B875 complex of SK101 under the light-limiting conditions was elevated by 20 to 30% compared with that of the puc -deleted cell, reflecting parallel increase of bacteriochlorophyll and carotenoid contents of the mutant. The formation of B875 complex of SK101 under the anaerobic dark conditions with dimethylsulfoxide was the same as that of the puc-deleted cell. suggesting that the mutation of SK101 result in the altered control of B875 complex formation by light. When puc is restored in SK101 , it is not B875 complex but B800-850 complex which formation is elevated. The mutation of SK101 affected the bchF transcription most drastically to show two to tenfold increase during both aerobic and photoheterotrophic growth. The mutated phenotype of SK101 was complemented with pW2, which contains approximately 100-kb HNA of the photosynthetic gene clusters. The complementing DNA was narrowed down to a 1.1-kb DNA containing orfQ and pufKBA . The mutation of SK101 appeared to be exerted through the mutation of the orfQ gene encoding a putative bacteriochlorophyll -mobilizing protein.

• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2003년도 생물공학의 동향(XII)
• /
• pp.409-411
• /
• 2003

한국의 서해안과 남해안 47개소의 해수와 mud 시료로부터 광합성세균으로 유추되는 13개의 균주를 분리하였고, agar-shake tube method와 RAPD-PCR을 이용하여 4종의 서로 다른 균주를 순수분리 하였다. 이들 4종의 균주 중 폐수분해능이 우수한 균주를 선정하여, 형태학적, 배양적, 생화학적 특성 및 16S rRNA sequencing에 의한 동정을 실시하였고, 그 결과 purple, non-sulfur bacteria의 Rhodobacter 속에 가장 근접하여, Rhodobacter sp. EGH-24로 명명하였다. Rhodobacter sp. EGH-24가 생산하는 carotenoid 색소는 spheroidene(group 2)이였고, bacteriochlorophyll a를 가지고 있었으며, PHB를 생산하는 것으로 확인되었다.

• 미생물학회지
• /
• 제30권6호
• /
• pp.495-500
• /
• 1992

Photosynthetic bacteria which can utilize ferulate as a sole carbon source for their metabolic activities were isolated from soils by liquid enrichment culture technique. The strain P-1 was selected by the highest capability of degrading ferulate in aerobic and anaerobic conditions. The strain P-1 was rod-shaped with its motility, strained gram negatively and could not utilize sulfur compounds. This strain has the bacteriochlorophyll a group I carotenoid and membrane structures like lamellae. As the results of physiological, morphological and cultural charactderistics, the isolate was identified as Rhodopseudomonas plaustris, one of the purple nonsulfer bacteria. The strain P-1 utilized 2mM/day in aerobic condition and 0.86 mM/day in anaerobic condition.

• Journal of Microbiology and Biotechnology
• /
• 제12권4호
• /
• pp.643-648
• /
• 2002

For wastewater treatment and utilization of the biomass, a photosynthetic bacterium was isolated based on its cell growth rate, cell mass, and assimilating ability of organic acids. The isolate was a Gram-negative rod-shaped bacterium that contained a single polar flagellum and formed a lamellar intracytoplasmic membrane (ICM) system, including bacteriochlorophyll $\alpha$. The major isoprenoid quinone component was identified as ubiquinone Q-10, and the fatty acid composition was characterized as to contain relatively large amount of C-16:0 (18.74%) and C-18:1 (59.23%). Based on its morphology, phototrophic properties, quinone component, and fatty acid composition, the isolate appeared to be closely related to the Rhodopseudomonas subgroup of purple nonsulfur bacteria. A phylogenetic analysis of the isolate using its 16S rRNA gene sequence data also supported the phenotypic findings, and classified the isolate closely related to Rhodopseudomonas palustris. Accordingly, the nomenclature of the isolate was proposed as Rhodopseudomonas palustris KUGB306. A bench-scale photosynthetic bacteria (PSB) reactor using the isolate was designed and operated for the treatment of soybean curd wastewater.

• Journal of Photoscience
• /
• 제9권2호
• /
• pp.350-352
• /
• 2002

We examined the bacteriochlorophyill/bacteriopheophytin ratios in several species of purple bacteria containing only LHI. The pigment ratios depended greatly on species. Further, Rhodospirillum rubrum showed wide variation when grown under different light intensity, and Rhodobium marinum showed significant variation from culture to culture even under the same light conditions. The protein ratios of a/RC and $\beta$/RC estimated by SDS-PAGE of chromatophores of Rsp. rubrum and Rbi. marinum exhibited the ratio of $\beta$/$\alpha$ > 1. These findings gave us the novel idea that there are two types of LHl; one is a C-shaped open antenna composed by $\alpha$$\beta$ units surrounding a RC, and another is a small closed ring antenna composed by $\alpha$$\beta$ units located peripherally in a variable ratio to the core complex like LH2.

• 한국환경과학회지
• /
• 제12권12호
• /
• pp.1293-1301
• /
• 2003

A species of facultative photo-organotrophic, purple, non-sulfur bacterium was isolated from the 47 point at west and south coast of Korea in September 2001. Separated 13 samples of changes with red color under 28-32$^{\circ}C$, 3000 lux, anaerobe conditions for 7 days cultivated in basal medium. For pure isolation from 13 samples, we used agar-shake tube method (0.4 ％ agar) and separated 5 strains through 13-repetition test. EGH-24 and EGH-30 was identified as the same strain through the RAPD(Random Amplified Polymorphic DNA)-PCR of strain EGH-9, EGH-13, EGH-23, EGH-24, EGH-30. Four isolates cultivated in synthesis wastewater for wastewater biodegradation test. EGH-24 was selected with efficient wastwater treating strain. Based on the results obtained from morphology, nutrient requirements, major bacteriochlorophyll content, 16S-rDNA phylogenetic analysis, EGH-24 strain may be identified as a new strain of the genus Rhodobacter and named Rhodobacter sp. EGH-24.

• Journal of Microbiology and Biotechnology
• /
• 제27권12호
• /
• pp.2173-2179
• /
• 2017

The intracytoplasmic membrane of Rhodobacter sphaeroides readily vesiculates when cells are lysed. The resulting chromatophore membrane vesicle (CMV) contains the photosynthetic machineries to synthesize ATP by ATPase. The light-dependent ATPase activity of CMV was lowered in the presence of $O_2$, but the activity increased to the level observed under anaerobic condition when the reaction mixture was supplemented with ascorbic acid (${\geq}0.5mM$). Cell lysis in the presence of biotinyl cap phospholipid (bcp) resulted in the incorporation of bcp into the membrane to form biotinylated CMV (bCMV), which binds to streptavidin resin at a ratio of approximately $24{\mu}g$ bacteriochlorophyll a/ml resin. The ATPase activity of CMV was not affected by biotinylation, but approximately 30% of the activity was lost by immobilization to resin. Interestingly, the remaining 70% of ATPase activity stayed constant during 7-day storage at $4^{\circ}C$. On the contrary, the ATPase activity of bCMV without immobilization gradually decreased to approximately 40% of the initial level in the same comparison. Thus, the ATPase activity of CMV is sustainable after immobilization, and the immobilized bCMV can be used repeatedly as an ATP generator.

• 미생물학회지
• /
• 제29권2호
• /
• pp.130-135
• /
• 1991

Trimorphomyces papilionaceus dikaryon 으로부터 자영적인 돌연변이에 의해 분리한 이배체를 대상으로 생장속도, U. V. 광 조사 후의 생존율, U. V. 분광광도계와 Hoechst 33258 핵염색에 의한 DNA 함량등을 monokaryon 및 dikaryon 과 비교하여 결정하였다. 이배체는 dikaryon 핵의 DNA 양과 비슷하였고 monokaryon 핵의 DNA 양의 2배 정도가 되었다. Glyoxalase II 의 band 양상에서 monokayon은 한 개의 isozyme band 만을 보인 반면, 이배체와 dikaryon은 같은 위치에서 두개의 isozyme band 가 관찰되었다. 이원전개한 전기영동실험에서 dikaryon 특이 단백질은 분자량이 66.000 보다 컸으며 분자량 24,000-29,000 사이에서 이배체 특이 단백질이 존재한다.