• ALGAE
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• 제26권2호
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• pp.153-165
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• 2011

Species of the diatom genus Chaetoceros with special intercalary setae are uncommon. For this study, we collected Chaetoceros species from August 2008 to September 2009 in Korean coastal waters and examined the ultra structures of the Chaetoceros species C. coarctatus, C. compressus var. hirtisetus, C. contortus, C. diversus, and C. messanensis, using light and scanning electron microscopy. C. coarctatus, in the subgenus Phaeoceros, showed longer and stronger spines than those found in other species. C. coarctatus and C. diversus had special intercalary setae with spines in straight arrangements, whereas C. compressus var. hirtisetus, C. contortus, and C. messanensis had special intercalary setae with spines arranged in spirals. The setae of C. coarctatus had spines that were robust toward the tips and, overall, longer and stronger than were those of other species. C. coarctatus and C. diversus were straight, and C. compressus var. hirtisetus, C. contortus, and C. messanensis spiraled. C. messanensis had two types of special intercalary setae, both forked: 1 with spines in spirals and 1 lacking spines. We did not find spines on the anterior part of divergent point of the special intercalary setae of C. messanensis. Foramina shapes of these 5 Chaetoceros species varied as follows: very small or no foramina in C. coarctatus, relatively wide and slightly centrally constricted foramina in C. compressus var. hirtisetus and C. contortus, quite narrowly slitted or no foramina in C. diversus, and lanceolate or hexagonal foramina in C. messanensis. We found rimoportula in both intercalary and terminal valves of C. coarctatus, but C. compressus var. hirtisetus, C. contortus, C. diversus, and C. messanensis only had rimoportula in terminal valves. In addition, C. compressus var. hirtisetus and C. contortus were new to Korean coastal waters.

• 한국물환경학회지
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• 제29권2호
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• pp.223-231
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• 2013

This study investigated the physio-chemical water quality and the standing crop of the phytoplankton of Dong-stream in Jeollanam-do from May 2010 to Feb 2011. The indicators and the dominant species of phytoplankton were also measured to investigate the water quality of estuary. Nutrients turned out to be eutrophic to hypertrophic in most sites with total nitrogen ranging 1.023~10.743 mg/L and total phosphorous ranging 0.0182~1.6848 mg/L. The site D6 had the highest BOD ranging 0.6~7.5 mg/L. Total of 205 taxa appeared within the research period, Bacillariophyceae being the highest number of taxa. The appeared species were taxa resistant to pollution including Navicula pupula, N. subminuscula, Nitzschia amphibia and N. palea. The downstream is thought to be greatly affected by the ocean water since marine and estuary phytoplankton such as Skeletonema costatum, Coscinodiscus radiatus, Bacteriastrum delicatulum and Eucampia biconcave appeared. Diatom appeared mainly as dominant species including Cymbella minuta, Melosira varians, Gomphonema parvulum, Stephanodiscus hantzschii. As for Stephanodiscus hantzschii, it dominates during fall and winter when the water temperature drops. Diatom showed 50% or higher dominant rate in all seasons at most sites. Most sites turned out to be eutrophic based on the standing crop of cells and chlorophyll a concentration. Total of 15 polluted water indicating taxa including Oscillatoria limosa appeared. From this result, the Dong-stream was considered to be in the eutrophication.

• 한국수산과학회지
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• 제34권5호
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• pp.556-562
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• 2001

본 연구에서는 비교적 성장이 빠르고 배양이 용이한 황갈편조 식물문 중 규조강에 속하는 P. tricornutum으로부터 선정하여 항 산화성 물질을 분리$\cdot$정제하여 물질의 구조를 밝혔다. P. tricornutum의 각 유기용매 추출물의 항산화성은 chloroform획분이 가장 좋았으며, 이 획분을 시료로 하여 silica gel column chromatography, PTLC 및 HPLC를 사용하여 항산화성 물질을 분리$\cdot$정제하였다. Silica gel column을 사용하여 분리된 획분의 항산화성은 dichloromethane: methanol (5: 1)로 용출시킨 획분에서 가장 높았으며, 이 획분의 PTLC 획분은 항산화성이 $20\%$ 증가하였다. 최종 정제된 물질의 $IC_50$값은 $8.3{\mu}g/mL$으로 화학적으로 합성된 항산화제인 BHT 및 BHA보다는 낮았지만 천연 항산화제인 $\alpha-tocopherol$보다는 다소 높게 나타났으며, 이 물질의 화학적 구조는 carotenoid류인 zeaxanthin으로 동정되었다.

• 한국환경보건학회지
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• 제23권1호
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• pp.18-27
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• 1997

The isolation, morphological study and growth characteristics of the algae were investigated from Lake Chuam. The isolated algae were applied the Agal Growth Potential test. The method of isolation and purification of the algae were used to Agar plating(AP), nutrient enrichment(NE), dilution(DI) and micro capillary technique(MC). Total isolated algae were 21 species. They were composed of Cyanophyceae, Dinophyceae, Bacillariophyceae, Euglenophyceae and Chlorophyceae. The numbers of algal strain by isolation technique were highest in dilution(21 species), and those of the rests were showed in order of NE > MC > AP. The sizes of isolated Selenastrum and Scenedesmus were $1.8\pm 1.4 \mu m$, $3.3\pm 0.9 \mu m$ in diameter and $6.4\pm 2.3 \mu m$, $13.6\pm 1.9 \mu m$ in length respectively. The morphology of isolated algae and NIES-collection strain was very similar each other, but the size was smaller isolated algae than that of NIES-collection. The optimum culture condition of isolated Selenastrum and Scenedesmus was about 30$\circ$C(25$\circ$C-35$\circ$C) in temperature and the maximum growth was appeared between 7,000 lux and 8,000 lux in the light intensity. The comparison of $\mu$(specific growth rate) on the concentration of nutrients such as nitrate and phosphate, isolated Selenastrum was appeared maximum it at 1.0 mg $NO_3-N/l$ but NIES-collection strain was showed 95% of maximum it at same nitrate concentration. Maximum g of isolated algae and NIES-collection strain in Scenedesmus onto nitrate concentration were very similar with the result of selenastrum. The specific growth rates of isolated algae and NIES-collection strain on the gradient concentration of phosphate were showed 0.72/day and 0.70/day at 0.02 mg $PO_4-P/l$ in Selenastrum but those of Scenedesmus were appeared 0.61/day and 0.57/day at same concentration $PO_4-P$.

• 한국환경과학회지
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• 제22권12호
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• pp.1683-1690
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• 2013

We investigated the spatial and temporal variations of phytoplankton in Youngil Bay as well as the effect of water physico-chemical parameters. Water samples at three stations were collected and measured monthly from May to November in 2010. The taxa of phytoplankton observed in this study were classified as 33 Bacillariophyceae, 23 Dinophyceae, 1 Euglenophyceae, 2 Crysophyceae and 1 Cryptophyceae. The highest biomass of phytoplankton was observed at inner station in September, which was characterized high concentration of dissolved inorganic phosphate(DIP) in surface water after rainfall. Nutrient concentrations, chlorophyll-a and phytoplankton biomass values showed the marked trend to decrease from the inner bay to the outer bay. Pearson's correlation co-efficient between salinity and other water parameters including chlorophyll-a, pH and DIP showed the strong negative relationship r=-0.82, r=-0.78 and r=-0.75 (p<0.01), respectively. These results indicate that the water quality of Youngil Bay could be stimulated by nutrient enriched input from Hyeogsan River discharge, and the spatial and temporal distribution of phytoplankton biomass principally limited to DIP concentration from Hyeogsan river.

• ALGAE
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• 제19권4호
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• pp.329-337
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• 2004

Monthly variations of phytoplankton communities were conducted to the investigation at five stations in mid and lower parts of the Nakdong River from December 1995 to November 1996. The phytoplankton communities were identified a total 456taxa which were composed of 136 genera, 427 species, 27 varieties and 2 forma. The standing crops of phytoplankton communities ranged from 2.7 to 52.8 (${\times}$10³)cells·ml$^{-1}$ during the investigation periods. Bacillariophyceae was the dominant classes at all stations. The dominant species were 7taxa and the standing crops of these ranged from 18.3 to 95.1% of the standing crops of phytoplankton communities. The dominant species in the mid parts (st. 1, 2) of this river system were benthic species such as Navicula gregaria, N. viridula var. rostellata and Nitzschia palea, however it was planktonic species (Stephan discus hantzschii, Golenkinia radiata) and meroplanktonic species (Aulacoseira ambigua, A. granulata) in the lower parts (st. 3, 4, 5). The seasonal variations of the dominant species in the lower parts were appeared to the planktonic species (S. hantzschii) from winter to spring, and were the meroplanktonic species (A. ambigua and A. granulata) in from summer to fall. The lower parts of the Nakdong river were entropic states because the dominant species were composed A. ambigua, A. granulate and S. hantzschii which were indicators of entropic water quality. The interrelationships between total standing crops and environmental factors(water temperature, pH, NH$_4$, NO$_2$, NO$_3$ and PO$_4$) were low positive or negative coefficients. S. hantzschii had low positive or negative relations with water temperature, NH$_4$, NO$_2$, NO$_3$ and PO$_4$. As the result, the variations of standing crops of phytoplankton were not caused by a single factor but controlled by the complex factors.

• ALGAE
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• 제20권4호
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• pp.333-343
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• 2005

Spatial and temporal variations of environmental factors and phytoplankton community were investigated in the three stations of Andong Reservoir in 1997 and 2003. The changes of physico-chemical water quality and phytoplanktonic biomass were higher in 2003 than that of 1997, due to rainfall difference. The concentration of total nitrogen (TN) and total phosphorus (TP) in the reservoir decreased, but total nitrogen fell relatively more between them. TN/TP ratio decreased from 109 to 90 showing no change at the downstream but a big decrease at the midand upstream. Predominant phylum of phytoplankton in Andong Reservoir were six genus that composed to Anabaena, Aphanizomenon and Microcystis of Cyanophyceae, Cosmarium and Scenedesmus of Chlorophyceae and Synedra of Bacillariophyceae, respectively. Among the observed phytoplankton, diatom Synedra occured as the maximum amount of 3,400 cells mL$^{-1}$ even at the above 30°C. Green algae Scenedesmus observed along with Microcystis. It seemed to be compete with Microcystis during the high water temperature period. Although trophic state of Andong Reservoir was decreased, the standing crops of phytoplankton were increased. Moreover bluegreen algae, Aphanizomenon and Microcystis in the region of upstream to midstream and diatom, Synedra in the region of midstream to downstream were increased until to reach the algal bloom, respectively. It seemed necessary to attention the changes of blue-green algae Aphanizomenon, that has an ability of nitrogen fixation.

• Ocean and Polar Research
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• 제32권1호
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• pp.1-13
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• 2010

To assess short-term variation of summer phytoplankton community structure in different water masses, phytoplankton and environmental factors were monitored from 31 stations on and off the southern coasts of Korea, from June 18 to June 20 2009. According to multidimensional scaling (MDS) and cluster analysis based on phytoplankton community data from each station, the southern sea was divided into two groups. The first group included stations in the south-eastern region of Jeju Island, which is strongly influenced by the Kuroshio warm current. The second group located along the coastal region of the southern sea, which was mainly comprised of Bacillariophyceae and Crytophyceae. Of these stations, St. 13 and 28 formed a temperature front caused by different hydrological conditions. In particular, nutrients and Chl.a concentrations in these two stations were significantly higher compared to those in the other stations. This indicates that phytoplankton population and subsequent microalgal growth under high nutrient concentrations vary in different water masses. Our results support the theory that phytoplankton community structure in the southern sea of Korea can be influenced on a short-term scale by different water masses and currents.

• ALGAE
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• 제22권3호
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• pp.193-199
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• 2007

This study was carried out to investigate the charateristics of environmental factors and the species compositions of phytoplanktons and periphytons in order to analyze the marine ecosystem (DOK1-3) with freshwater (Mul) in the coast of Dokdo. Mean values of conductivity (TSD) (44.3 mS cm–1), total dissolved solids (30.9 mg L–1), salinity (31.8 psu), total suspended solids (39.2 mg L–1), and ash-free dry matter (AFDM) (8.8 mg L–1) were higher in DOK1- 3 than those in Mul (3.2 mS cm–1, 2.3 mg L–1, 1.9 psu, 2.8 mg L–1 and 2.8 mg L–1, espectively), while mean amounts of soluble reactive phosphorus (SRP) (7.5 μg P L–1) and soluble reactive silicon (SRS) (0.1 μg Si L–1) were significantly lower in DOK1-3 than those in Mul (117.3 μg P L–1 and 4,105 μg Si L–1, respectively). The phytoplanktons was a total of 59 species in Dokdo, which were composed of 1 species (1.7%) for Chrysophyceae, 43 species (72.9%) for Bacillariophyceae and 15 species (25.4%) for Dinophyceae. The biomass (Chl-a, phaeopigment and AFDM) of epilithic algae (ELA), epizooic algae (EZA) and epiphytic algae (EPA) were varied with the kinds of substrates (EPA > ELA > EZA) in the whole stations. Water environments was comparatively clean in the coast of Dokdo as comparing with those of the land. However, the monitoring of marine ecosystem in the Dokdo should be continued for species conservation according to the global warming by the climatic change.

• ALGAE
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• 제33권2호
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• pp.191-203
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• 2018

Fragilariopsis cylindrus is one of the most successful psychrophiles in the Southern Ocean. To investigate the molecular mechanism of biomineralization in this species, we attempted to synchronize F. cylindrus growth, since new cell wall formation is tightly coupled to the cell division process. Nutrient limitation analysis showed that F. cylindrus cultures rapidly stopped growing when deprived of silicate or light, while growth continued to a certain extent in the absence of nitrate. Flow cytometry analysis indicated that deprivation of either silicate or light could effectively arrest the cell cycle of this diatom species at the G1 phase, suggesting that synchrony can be established using either factor. Fluorescence labeling of new cell walls was faintly detectable as early as approximately 6 h after silicon repletion or light irradiation, and labeling was markedly intensified by 18 h. It is revealed that the synthesis of girdle bands begins before valve synthesis in this species, with active valve synthesis occurring during the G2 / M phase. Expression profiling revealed that selective member(s) of the F. cylindrus SIT genes (FcSIT) respond to silicate and light, with a different set of genes being responsive to each factor. The Si / light double depletion experiments demonstrated that expression of one FcSIT gene is possibly correlated to transition to G2 / M phase of the cell cycle, when the valve is actively formed.