• Transactions of the Korean Society of Mechanical Engineers B
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• v.27 no.4
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• pp.440-446
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• 2003

Steady-state structure and acoustic pressure responses of GH$_2$-LOx diffusion flames in stagnation-point flow configuration have been studied numerically with a detailed chemistry to investigate the acoustic instabilities. The Rayleigh criterion is adopted to judge the instability of the GH$_2$-LOx flames from amplification and attenuation responses at various acoustic pressure oscillation conditions for near-equilibrium to near-extinction regimes. Steady state flame structure showed that the chain branching zone is embedded in surrounding two recombination zones. The acoustic responses of GH$_2$-LOx flame showed that the responses in near-extinction regime always have amplification effect regardless of realistic acoustic frequency. That is, GH$_2$-LOx flame near-extinction is much sensitive to pressure perturbation because of the strong effect of a finite-chemistry.

• Asian-Australasian Journal of Animal Sciences
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• v.11 no.4
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• pp.422-427
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• 1998

The gene and genotypic frequencies of ${\kappa}$-casein (${\kappa}$-CN), ${\beta}$-lactoglobulin (${\beta}$-LG), growth hormone (bGH) and prolactin (bPRL) loci in Korean cattle were investigated using PCR-RFLP analyses. Genomic DNA samples were obtained from 290 cows and 30 AI bulls. In both cows and bulls, the most predominant genotypes of ${\kappa}$-CN, ${\beta}$-LG, bGH and bPRL loci were AB, BB, AA and AA, respecitively. The frequencies of A and B alleles for ${\kappa}$-CN locus were .612 and .388 for cows and .567 and .433 for bulls. The respective frequencies of A and B alleles for ${\beta}$-LG locus were .153 and .847 in cows and .217 and .783 in bulls. The frequencies of A and B alleles for bGH locus were .769 and .231 in cows and .784 and .216 in bulls, respectively. The frequencies of A and B alleles for bPRL locus were .678 and .322 for cows and .767 and .233 for bulls. Differences in frequencies of these alleles were not significant between cows and bulls at all loci examined. If the DNA polymorphisms of these candidate genes are associated with economically important traits, they could serve as genetic markers for genetic improvement in future marker-assisted selection programs in Korean cattle.

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.10
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• pp.1359-1364
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• 2013

The purpose of this study was to find any association of the bovine growth hormone (bGH) gene with growth and carcass quality traits in Korean native cattle, Hanwoo. Genomic DNA was extracted from 21 Hanwoo individuals, and the 47 to 2,528 bp region of the bGH 2,856 bp (GenBank accession number M57764) including the promoter and the five exons was sequenced. A total of ten bGH SNPs were confirmed, including four (253 C>T, 303 C>T, 502 C>T, and 559 G>A) in the promoter, one (679 C>T) in exon 1, one (1,692 T>C) in intron 3, and four (2141 C>G, 2258 C>T, 2277 C>T, and 2291 A>C) in exon 5. The ten bGH SNPs were genotyped for a sample of 242 Hanwoo steers and association tests were performed to find any significant SNP that was correlated with growth and carcass quality. Of the SNPs, the 303 C>T SNP in the promoter region was significantly associated with 6-month-old weight, the 559 G>A SNP with longissimus dorsi muscle area, the 2141 C>G SNP in exon 5 with daily weight gain, and the 2258 C>T SNP with daily weight gain and carcass weight (p<0.05). The significant SNPs need to be verified in other Hanwoo populations before considering implementation of marker-assisted selection for genetic improvement of growth and carcass quality in Hanwoo.

• Korean Journal of Microbiology
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• v.27 no.2
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• pp.85-91
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• 1989

The effect of SV40 and murine cytomegalovirus (MCMV) enhancers on the general and tissue-specific gene expression was investigated. Recombinant plasmids containing these transcriptional engancers downstream of a structural gene for chloramphenicol acetyl transferase (CAT) were constructed. The transient expression of CAT gene from these plasmids was measured in monkey (CV1PD) and HeLa cells. Both SV40 and MCMV engancers activated the expression of CAT gene by more than 20 and 150 folds, respectively, compared with engancerless plasmids. When the SV40 promoter, upstream of CAT gene, was replaced with 2.2 kbp of promoter regulatory region of bovine growth hormone (bGH) gene, there was no expression of CAT even in the presence of enhancers, reflecting the tissue-specific expression of bGH genes. However, when the bGH regulatory region was shortened to 230 bp, the expression level increased dramatically in the presence of SV40 enhancers. In contrast, the expression from the shortened promoter was only marginally activated by the stronger MCMV enhancer.

• Journal of Korean Society of Water and Wastewater
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• v.27 no.6
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• pp.771-778
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• 2013

Gas hydrate (GH) process is a new desalination technology, where GH is a non- stoichiometric crystalline inclusion compounds formed by water and a number of gas molecules. Seawater GH is produced in a low temperature and a high pressure condition and they are separated from the concentrated seawater. The drawback of the GH process so far is that salt contents contained in its product does not meet the fresh water quality standard. This means that the GH process is not a standalone process for seawater desalination and it needs the help of other desalting process like reverse osmosis (RO). The objective of this study is to investigate the effect of GH process on energy saving for RO process in seawater desalination. The GH product water quality data, which were obtained from a literature, were used as input data for RO process simulation. The simulation results show that the energy saving effect by the GH process is in a range of 68 % to 81 %, which increases as the salt removal efficiency of the GH process increases. Boron (B) and total dissolved solids (TDS) concentrations of the final product of the hybrid process of GH and RO were also investigated through the RO process simulation to find relavant salt rejection efficiency of the GH process. In conclusion, the salt rejection efficiency of the GH process should exceed at least 78% in order to meet the product water quality standards and to increase the energy saving effect.

• Fisheries and Aquatic Sciences
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• v.1 no.1
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• pp.19-23
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• 1998

Effects of pituitary and thyroid hormones on estradiol-induced vitellogenin (VTG) induction were electrophoretically examined in primary hepatocyte cultures of rainbow trout. Hepatocytes were precultured for 2 days and then estradiol-17 $\beta$ $(E_2,\;2 \times 10^{-6}M)$>, triiodothyronine $(T_3,\;10^{-8}-10^{-6}M)$, bovine growth hormone (bGH, 10-100 ng/ml), ovine prolactin (oPRL, 100-500 ng/ml), and pituitary extract (PE) of rainbow trout (0.75PE/dish) were added to the incubation medium. The hepatocytes were cultured for 7 more days. The addition of oPRL to the incubation medium was not effective in increasing VTG production at any concentrations. The addition of PE to the incubation medium with $E_2$ was not effective in increasing VTG production. The addition of bGH to the incubation medium with $E_2$ was not effective in increasing the rate of VTG production at concentrations of 10-50 ng/ml. However, a higher concentration of bGH, 100 ng/ml, increased VTG production. The various concentrations of $T_3$ were ineffective in stimulating VTG production. These results suggest that GH could be one of stimulus factors for VTG production in rainbow trout.

• Korean Journal of Agricultural Science
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• v.38 no.3
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• pp.541-547
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• 2011

Korean building industry was developed by concrete and steel construction. However, concrete and steel have some problems which low carbon storage capability and difficulty of recycling. According to many studies, timber has high carbon storage capability, high recycling capability and sustainable supporting capability. Focus on this factors of timber, make new wall structure as Green Timber Wall panels and check the shear performance to use wall system in housing construction such as light-weight timber construction and nondearing wall on other construction. In the results, B-4-B and B-4-S show similar modulus of shear stiffness on the table. GH-4-GH has slip shape failure mode between Green Timber Wall boards. GH-4-GV has most stable characteristic curve than other specimens.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.4
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• pp.573-583
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• 2015

B-32 is one of a panel of monoclonal anti-idiotypic antibodies to growth hormone (GH) that we developed. To characterize and identify its potential role as a novel growth hormone receptor (GHR) agonist, we determined that B-32 behaved as a typical $Ab2{\beta}$ based on a series of enzyme-linked immunosorbent assay assays. The results of fluorescence-activated cell sorting, indirect immunofluorescence and competitive receptor binding assays demonstrated that B-32 specifically binds to the GHR expressed on target cells. Next, we examined the resulting signal transduction pathways triggered by this antibody in primary porcine hepatocytes. We found that B-32 can activate the GHR and Janus kinase (2)/signal transducers and activators of transcription (JAK2/STAT5) signalling pathways. The phosphorylation kinetics of JAK2/STAT5 induced by either GH or B-32 were analysed in dose-response and time course experiments. In addition, B32 could also stimulate porcine hepatocytes to secrete insulin-like growth factors-1. Our work indicates that a monoclonal anti-idiotypic antibody to GH (B-32) can serve as a GHR agonist or GH mimic and has application potential in domestic animal (pig) production.

• Korean journal of applied entomology
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• v.54 no.3
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• pp.159-167
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• 2015

In order to establish B. tabaci control in paprika greenhouses a fixed-precision-level sampling plan was developed. The sampling plan consisted of spatial distribution analysis, a sampling stop line, and decision making. Sampling was conducted simultaneously in two independent greenhouses (GH 1, GH 2). GH 1 and 2 were surveyed every week for 22 consecutive weeks, using 19 sampling locations in GH 1 and 9 sampling locations in GH 2. The plant in both greenhouses were divided into top (180-220 cm from the ground), middle (80-120 cm from the ground) and bottom (30-70 cm from the ground) sections and B. tabaci adults and pupae were observed on three paprika leaves at each position and recorded separately. GH 2 data were used to validate the fixed-precision sampling plan, which was developed using GH 1 data. In this study, spatial distribution analysis was performed using Taylor's power law with the pooled data of the top and bottom position (B. tabaci adults), and the middle and bottom positions (B. tabaci pupae), based on a 1-leaf sampling unit. Decision making was undertaken using the maximum of action threshold in accordance with previously published method, and the value was decided by the price of the plants. Using the results obtained in the greenhouse, simulated validation of the developed sampling plan by RVSP (Resampling Validation for Sampling Plan) indicated a reasonable level of precision.

• The Korean Journal of Pesticide Science
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• v.20 no.3
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• pp.189-196
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• 2016

Several microorganisms in particular Bacillus subtilis group have been isolated from diverse places such as soils and the gastrointestinal tract of ruminants etc., and used as biocontrol agent against various plant pathogens and utilized as plant growth promoting agents. Among them, Bacillus is well known as one of the most useful bacteria for biocontrol and plant growth promotion. Bacterium GH1-13 was isolated from a reclaimed paddy field in Wando Island and identified as Bacillus velezensis using phylogenetic analysis on the basis of 16S rRNA and gyrB gene. It was confirmed that GH1-13 produced indole acetic acid (IAA) associated with promoted growth of rice root. GH1-13 showed characteristics of antagonization against the main pathogen of rice as well as diverse pathogenic fungi. GH1-13 had biosynthetic genes, bacillomycin, bacilycin, fengycin, iturin, and surfactin which are considered to be associated closely with inhibition of growth of pathogenic fungi and bacteria. This study showed that GH1-13 could be used as a multifunctional agent for biocontrol and growth promotion of crop.