• Journal of Microbiology and Biotechnology
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• v.16 no.11
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• pp.1773-1777
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• 2006

We evaluated the usefulness of DNA microarray as a comparative genomics tool, and tested the validity of the cutoff values for defining absent genes in test genomes. Three genome-sequenced E. coli strains (K-12, EDL933, and CFT073) were subjected to comparative genomic hybridization with DNA microarrays covering almost all ORFs of the reference strain K-12, and the microarray results were compared with the results obtained from in silico analyses of genome sequences. For defining the K-12 ORFs absent in test genomes (reference strain-specific ORFs), we applied and evaluated the cutoff level of -1. The average sequence similarity between ORFs, to which corresponding spots showed a log-ratio of>-1, was $96.9{\pm}4.8$. The numbers of spots showing a log-ratio of <-1 (P<0.05, t-test) were 90 (2.5%) and 417 (10.6%) for the EDL933 genome and the CFT073 genome, respectively. Frequency of false negatives (FN) was ca. 0.2, and the cutoff level of -1.3 was required to achieve the FN of 0.1. The average sequence similarity of the false negative ORFs was $77.8{\pm}14.8$, indicating that the majority of the false negatives were caused by highly divergent genes. We concluded that the microarray is useful for identifying missing or divergent ORFs in closely related prokaryotic genomes.

• KCI Concrete Journal
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• v.13 no.1
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• pp.19-25
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• 2001

Based on the orthotropic hypoelasticity formulation, a constitutive material model of concrete taking account of triaxial stress state is presented. In this model, the ultimate strength surface of concrete in triaxial stress space is described by the Hsieh's four-parameter surface. On the other hand, the different ultimate strength surface of concrete in strain space is proposed in order to account for increasing ductility in high confinement pressure. Compressive ascending and descending behavior of concrete is considered. Concrete cracking behavior is considered as a smeared crack model, and after cracking, the tensile strain-softening behavior and the shear mechanism of cracked concrete are considered. The proposed constitutive model of concrete is compared with some results obtained from tests under the states of uniaxial, biaxial, and triaxial stresses. In triaxial compressive tests, the peak compressive stress from the predicted results agrees well with the experimental results, and ductility response under high confining pressure matches well the experimental result. The reinforcing bars embedded in concrete are considered as an isoparametric line element which could be easily incorporated into the isoparametric solid element of concrete, and the average stress - average strain relationship of the bar embedded in concrete is considered. From numerical examples for a reinforced concrete simple beam and a structural beam type member, the stress state of concrete in the vicinity of talc critical region is investigated.

• Journal of Biosystems Engineering
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• v.18 no.4
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• pp.371-381
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• 1993

Threshing operation is performed by impact, compression and friction forces inside the thresher. These values should be appropriate to the crop condition to enhance the threshing and separating efficiency and to decrease the grain damage. To analyze the threshing process inside the rasp-bar type thresher, impact, friction and compression forces were measured using transducers with strain gage circuits. To measure the impact forces and friction forces between the rasp-bar and crop, full bridge strain gage circuit was built on the rasp-bar holder. To measure the compression forces and circumferential friction forces between the concave and crop, two sets of full bridge strain gage circuits were built on the T-type concave transducer. Threshing work of wheat crop with 12% of moisture content was performed at 3 levels of compression ratio and with 3 replications. Each transducer could not measure the exact forces continuously because the transducer oscillates with the forces. However they could measure maximum forces and force distribution according to the time. Average friction coefficients between crop and concave was 0.61 not showing any significant difference according to the compression ratio. Average acceleration of the crop in the cylinder appeared from $70.6m/s^2$ to $140.8m/s^2$ according to the compression ratio. The velocity of the crop at the exit of the cylinder appeared from 10.7m/s to 15.0m/s according to the compression ratio.

• Journal of the Korean Society for Heat Treatment
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• v.30 no.6
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• pp.258-263
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• 2017

The effect of cooling rate on the damping capacity of pure Mg was studied. Two Mg samples with different cooling rates were prepared by heat treatment at 873 K for 24 h, followed by water quenching and by furnace cooling to room temperature, respectively. The average grain sizes of the Mg samples were almost identical regardless of the cooling rate, but more twins were observed in the sample with faster cooling rate. The calculated vacancy fraction was higher in the fast cooling sample than the slow cooling one. It is noted that the fast cooling sample exhibited lower damping capacity both in the strain-amplitude independent and strain-amplitude dependent regions. Higher values of vacancy concentration and number density of twins in the fast cooling sample are considered to be responsible for the deteriorated damping capacity in the strain-amplitude independent and strain-amplitude dependent regions, respectively.

• Proceedings of the Korean Society of Machine Tool Engineers Conference
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• 1999.10a
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• pp.63-68
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• 1999

Because misaligned shafts have caused noise, vibration, bearing failures, and stress concentration of coupling part, which decrease the efficiency and life of a shaft system, the proper alignment of shaft system should be monitored continuously in dynamic condition. To solve these problems under dynamic condition, a telemetry system is this study is used to find the condition of the least bending moment, which is known by analyzing the structure and stress induced by misalignment is investigated. The moment derived from two shaft strain at the nearby coupling is measured. The bending strain is measured 5 times for average in static state as well as dynamic state with 100~700 rpm.

• 한국연소학회:학술대회논문집
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• 2003.12a
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• pp.115-123
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• 2003

Simultaneous measurements of velocity and OH distribution were made using particle image velocimetry(PIV) and planar laser-induced fluorescence(PLIF) of OH radical in turbulent hydrogen nonpremixed flames with coaxial air. The OH radical was used as an approximate indicator of chemical reaction zone. The OH layer was correlated well with the stoichiometric velocity, $U_s$, instantaneously and on average. In addition, high strain-rate regions almost coincide with the OH distribution. The residence time in flame surface, calculated from the root-mean-square value of the radial velocity, is proportional to $(x/d_F)^{0.7}$. It is found that the mean value of principal strain rate on the OH layer can be scaled with $(x/d_F)^{-0.7}$ and therefore, the product of the residence time and the mean strain rate remains constant over all axial positions.

• Microbiology and Biotechnology Letters
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• v.22 no.4
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• pp.373-381
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• 1994

An actinomycete strain, HSL-613 was isolated -from soil and identified by International Streptomyces Project (ISP) and chemotaxonomic methods. The spore chain of the strain HSL-613 appears in a spiral shape, and its spores are spherical shape with smooth surface. The cell wall contains LL-diaminopimelic acid (DAP). Menaquinone MK-9 (H$_{6}$, H$_{8}$) and iso- and anteiso-branched fatty acids were detected from whole cell extract. Sugars identified from whole cell extract include galactose, glucose, mannose and ribose, which are distinct from general sugar patterns of Streptomyces. Average G+C content in the chromosome is 59%. 5S rRNA of HSL-613 consists of 120 nucleotides as determined by comparing with that of a type strain Streptomyces griseus subsp. KCTC 9080. Through morphological, physiological, and chemical characterization, HSL-613 was identified and named as Streptomyces sp. HSL-613.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2007.10a
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• pp.96-99
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• 2007

The microstructure with fine and uniform AGS(austenite grain size) along thickness direction over no recrystallization temperature is strongly required for production of the high strength steels. The previous AGS prediction only based on the average strain improves to find the rolling conditions for accomplishment of the fine grain, but cannot find those for uniform grain. In this paper, an integrated mathematical model for prediction of the strain distribution along thickness direction is developed by carrying out finite element simulation for a series of rolling conditions. Also, the AGS distribution after rough rolling is predicted by applying the proposed model with AGS prediction model.

• Journal of Animal Science and Technology
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• v.63 no.3
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• pp.666-670
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• 2021

Paenibacillus konkukensis sp. nov., SK3146 is a novel strain isolated from a pig feed. Here, we present complete genome sequence of SK3146. The genome consists of a single circular genome measuring 7,968,964 bp in size with an average guanine + cytosine (G+C) content of 53.4%. Genomic annotation revealed that the strain encodes 151 proteins related to hydrolases (EC3), which was higher than those in Bacillus subtilis and Escherichia coli. Diverse kinds of hydrolases including galactosidase, glucosidase, cellulase, lipase, xylanase, and protease were found in the genome of SK3146, coupled with one bacteriocin encoding gene. The complete genome sequence of P. konkukensis SK3146 indicates the immense probiotic potential of the strain with nutrient digestibility and antimicrobial activity functions.

• Proceedings of the Korea Concrete Institute Conference
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• 2002.10a
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• pp.167-172
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• 2002

Nonlinear analysis of the reinforced concrete beam subjected to torsion is presented. Seventeen equations involving seventeen variables are derived from the equilibrium equation, compatibility equation, and the material constitutive laws to solve the torsion problem. Newton method was used to solve the nonlinear simultaneous equations and efficient algorithms are proposed. Present model covers the behavior of reinforced concrete beam under pure torsion from service load range to ultimate stage. Tensile resistance of concrete after cracking is appropriately considered. The softened concrete truss model and the average stress-strain relations of concrete and steel are used. To verify the validity of Present model, the nominal torsional moment strengths according to ACI-99 code and the ultimate torsional moment by present model are compared to experimental torsional strengths of 55 test specimens found in literature. The ultimate torsional moment strengths by the present model show good results.