• Plant Biotechnology Reports
• /
• v.3 no.4
• /
• pp.277-283
• /
• 2009

One of the limitations to conducting maize Agrobacterium-mediated transformation using explants of immature zygotic embryos routinely is the availability of the explants. To produce immature embryos routinely and continuously requires a well-equipped greenhouse and laborious artificial pollination. To overcome this limitation, an Agrobacterium-mediated transformation system using explants of type II embryogenic calli was developed. Once the type II embryogenic calli are produced, they can be subcultured and/or proliferated conveniently. The objectives of this study were to demonstrate a stable Agrobacterium-mediated transformation of maize using explants of type II embryonic calli and to evaluate the efficiency of the protocol in order to develop herbicide-resistant maize. The type II embryogenic calli were inoculated with Agrobacterium tumefaciens strain C58C1 carrying binary vector pTF102, and then were subsequently cultured on the following media: co-cultivation medium for 1 day, delay medium for 7 days, selection medium for $4{\times}14$ days, regeneration medium, and finally on germination medium. The T-DNA of the vector carried two cassettes (Ubi promoter-EPSPs ORF-nos and 35S promoter-bar ORF-nos). The EPSPs conferred resistance to glyphosate and bar conferred resistance to phosphinothricin. The confirmation of stable transformation and the efficiency of transformation was based on the resistance to phosphinothricin indicated by the growth of putative transgenic calli on selection medium amended with $4mg\;1^{-1}$ phosphinothricin, northern blot analysis of bar gene, and leaf painting assay for detection of bar gene-based herbicide resistance. Northern blot analysis and leaf painting assay confirmed the expression of bar transgenes in the $R_1$ generation. The average transformation efficiency was 0.60%. Based on northern blot analysis and leaf painting assay, line 31 was selected as an elite line of maize resistant to herbicide.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.52 no.3
• /
• pp.341-347
• /
• 2007

Chinese milk vetch (CMV) is a winter legume that is commonly used as cover crop in Korea. Kill date of cover crop for addition into soil affects N content in cover crop and N availability in soil. This study was conducted to evaluate the effect of CMV as green manure cover crop according to kill dates before growing corn without artificial fertilizer. Top of CMV cut three times on 13 April, 27 April, and 11 May were added into soil at a rate of 600 kg per 10a. Sugar content in CMV litter was persistently decreased from mid-April to late-May. The decrease of sugar content might be due to the transformation into starch and/or other storage or structural constituents. The decreased amount of sugars was greater than 12% and the increased amount of starch was less than 0.2%. Concentration of $NH_4^+$ in soil treated by CMV litter cut on May 11 was slightly higher than that in the treatment with early-cut (April 13) CMV, the concentration at 28 and 49 DAT (days after treatment) was higher in the treatment with late-cut CMV litter. Regardless of cut (kill) date of CMV, the phosphatase activity in the treatment of CMV litter was higher compared to the untreated control. Soil dehydrogenase activity was increased steadily by addition of CMV litter implying total microbial activities in the soil were increased. Our results demonstrate that the status of cover crop species at kill date is an important factor influencing soil enzyme activities derived from microorganisms. Therefore, the optimal kill date of cover crop should be examined to improve the efficiency of cover crop as green manure crop regarding the practical sequence in cropping system.

• Journal of KIISE:Computing Practices and Letters
• /
• v.9 no.6
• /
• pp.718-731
• /
• 2003

SAN(Storage Area Network) was developed in response to the requirements of high availability of data, scalable growth, and system performance. In order to use the SAN more efficiently, most of the SAN operating software supports storage virtualization concepts that allow users to view physical storage devices of the SAN as a large volume. A logical volume manager plays a key role in storage virtualization. It realizes the storage virtualization by mapping logical addresses to physical addresses. In this paper, we design and implement an efficient and flexible mapping method for the logical volume manager. Additionally we also design and implement a free space management method for flexible mapping. Our mapping method supports a snapshot that preserves a volume image at certain time and on-line reorganization to allow users to add or remove storage devices to and from the SAN even while the system is running. To justify our mapping method, we compare it with the mapping method of the GFS (Global File System) through various experiments.

• Journal of Biosystems Engineering
• /
• v.40 no.2
• /
• pp.124-136
• /
• 2015

Purpose: Grain quality is a general concept that covers many characteristics, ranging from physical to biochemical and physiochemical properties. Rice aging during storage is currently a challenge in the rice industry, and is a complicated process involving changes in all of the above properties. Spectroscopic techniques can be used to obtain information on the quality of rice samples in a non-destructive manner. Methods: The objective of this review was to highlight the factors that contribute to rice quality and aging, and to describe various spectroscopic modalities, particularly vibrational and hyperspectral imaging, for the assessment of rice quality. Results: Starch and protein are the main components of the rice endosperm, and are therefore key factors contributing to eating and cooking quality. While the overall starch, protein, and lipid content in the rice grain remains essentially unchanged during storage, structural changes do occur. These changes affect pasting and gel properties, and ultimately the flavor of cooked rice. In addition, grain quality is significantly affected by growing and environmental conditions, such as water availability, temperature, fertilizer application, and salinity stress. These properties can be evaluated using spectroscopic techniques, and rice samples can be discriminated by using multivariate statistical analysis methods. Conclusion: Hyperspectral imaging and vibrational spectroscopy techniques have good potential for determining rice quality properties in a non-invasive manner, i.e., not requiring the introduction of instruments into the rice grain.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• v.8 no.5
• /
• pp.1726-1743
• /
• 2014

DDoS (Distributed Denial of Service) has been a continuous threat to the cyber world with the growth in cyber technology. This technical evolution has given rise to a number of ultra-sophisticated ways for the attackers to perform their DDoS attack. In general, the attackers who generate the denial of service, use the vulnerabilities of the TCP. Some of the vulnerabilities like SYN (synchronization) flooding, and IP spoofing are used by the attacker to create these Distributed Reflected Denial of Service (DrDoS) attacks. An attacker, with the assistance of IP spoofing creates a number of attack packets, which reflects the flooded packets to an attacker's intended victim system, known as the primary target. The proposed scheme, Efficient Spoofed Flooding Defense (ESFD) provides two level checks which, consist of probing and non-repudiation, before allocating a service to the clients. The probing is used to determine the availability of the requested client. Non-repudiation is taken care of by the timestamp enabled in the packet, which is our major contribution. The real time experimental results showed the efficiency of our proposed ESFD scheme, by increasing the performance of the CPU up to 40%, the memory up to 52% and the network bandwidth up to 67%. This proves the fact that the proposed ESFD scheme is fast and efficient, negating the impact on the network, victim and primary target.

• Proceedings of the Korea Society for Industrial Systems Conference
• /
• 2002.11a
• /
• pp.520-532
• /
• 2002

A new architecture called the Storage Area Network(SAN) was developed in response to the requirements of high availability of data, scalable growth, and system performance. In order to use SAN more efficiently, most SAN operating systems support storage virtualization concepts that allow users to view physical storage devices attached to SAN as a large volume virtually. A logical volume manager plays a key role in storage virtualization. In order for mapping managers to process snap-shots and reorganizations, an efficient freespace manager is required, and it affects the overall performance of logical volume. In this thesis, we design and implement a freespace manager for logical volume manager. The freespace manager efficiently allocates physical blocks for logical blocks. Also, it processes space allocation requests for supports snapshots and reorganizations.

• 한국지구물리탐사학회:학술대회논문집
• /
• 2008.10a
• /
• pp.13-20
• /
• 2008

Magnetic method is rapid, cheap and simple geophysical exploration technique, and has wide range of applications such as resources prospecting, geological structure investigation and even geotechnical and environmental problems. Documents during Japanese occupation says that magnetic method was used for exploring metallic ore deposits and hot spring, and that a geomagnetic observatory was operated. From mid 1950's, magnetic explorations for natural resources such as metallic ore, uranium, coal, and groundwater were intensively executed for industrialization. Magnetic survey techniques were rapidly advanced during 1970's and 1980's with improvements of instruments, growth of geophysical manpower, and availability of computers. Decline of mining industry since mid 1980's moved the exploration objects from traditional resources to new ones such as groundwater and geothermal resources. Recently appeared applications such as natural hazard assessment, and engineering and environmental studies increased the magnetic method's utility in the realm of exploration.

• KIISE Transactions on Computing Practices
• /
• v.21 no.1
• /
• pp.46-51
• /
• 2015

The explosive growth of data and the rapidly changing technical social evolution new analysis paradigm for predicting and reacting the future the past and present ig data. Prescriptive analysis has a fundamental difference because can support specific behaviors and results according to user's goals with defin researchers establish judgments and activities achiev the goals. However research methods not widely implemented and even the terminology, Prescriptive analysis, is still unfamiliar. This paper thus propose an infrastructure in the prescriptive analysis field with key considerations for enhancing capability of researchers through a case study based on InSciTe Advisory developed with scientific big data. InSciTe Advisory system s developed in 2013, and offers a prescriptive analytics report which contains various As-Is analysis results and To-Be analysis results 5W1H methodology. InSciTe Advisory therefore shows possibility strategy aims to reach a target role model group. Through the availability and reliability of the measurement model the evaluation results obtained relative advantage of 118.8% compared to Elsevier SciVal.

• Korean Journal of Food Science and Technology
• /
• v.8 no.4
• /
• pp.263-272
• /
• 1976

According to the intercombined review of chemical and biological investigation it has been noted that the metabolizable energy per gram dietary protein of mixed diet of daily intake patterned by Korean population has been found 3.4-3.6 Cal., which entails 10-12% level of the protein calorie percentage of total metabolizable energy, the biological value being fallen within the scope 63-73. The structure of dietary protein has revealed that the lysine and isoleucine were primary limiting amino acids and threonine secondary limiting as a general trend, however, it is assumed that the ultimate nutritional effect of dietary protein might be restricted uniformly among regions by the amount of lysine, since the lysine availability has been yielded as low as 72-82% level. As for the net protein utillization NPUst falls in the range of 52-62 and the NPUop 47-58. In either part the mountainous region has demonstrated lowest value and the urban area highest, these trend being obviously associated with the ratio of animal protein relative to the vegetable origin. The net dietary protein calorie percentage (NDpCal %) has been found within the range of 5-7 that may be capable of meeting the requirement for the maintenance of adult, though for the growth it is insufficient. Present level of total caloric intake would not influence on the fate of protein value of prevailing regional diet in terms of caloric restriction, since the present intake of food energy is higher than the lower limit of caloric intake that would impair the biological performance of dietary protein fed ad libitum basis. Based on the protein efficiency, the adequacy of current level of protein intake was analyzed in terms of utilizable protein, and it has been demonstrated that the 37.8g of utilizable protein in the fishery region and 38.2g in the mountainous region were bellow the FAO recommendation. Accordin to the hematological study it may be interpreted that the anemic symptoms of the mountainous region has some possibility of being related to the inferior status of dietary protein in quality as well as in quantity.

• 전자공학회논문지 IE
• /
• v.47 no.1
• /
• pp.56-60
• /
• 2010

The widespread development of IT, growth of Web 2.0 application, the proliferation of personal hand held devices with access to the internet, and the availability of wireless networks, each have played an important role in creating the cloud computing model. Cloud computing is a business model and new trend of web application technology. The term is often used in the same context as grid computing or utility computing. In the cloud computing environment, we are able to use the same all of hardware resources in the server and share information easily. In this paper, we aimed a study to apply e-learning part to cloud computing environment. For this purpose, we proposed an application of LMS and LCMS based e-learning in the cloud computing environment. So LMS including LCMS connected to data center of cloud computing.