• BMB Reports
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• v.28 no.6
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• pp.527-532
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• 1995

Human taurine transporter has 12 transmembrane domains and its molecular weight is 69.6 kDa. The long cytoplasmic carboxy and amino termini might function as regulatory attachment sites for other proteins. Six potential protein kinase C phosphorylation sites have been reported in human taurine transporter. In this report, we studied the effects of phorbol 12-myristate 13-acetate (PMA) and glucocorticoid hormone on taurine transportation in the RAW 264.7, mouse macrophage cell line. When the cells were incubated with $[^{3}H]taurine$ in the presence or absence of $Na^+$ ion for 40 min at $37^{\circ}C$, the [$[^{3}H]taurine$ uptake rate was 780-times higher in the $Na^{+}-containing$ buffer than in the $Na^{+}-deficient$ buffer, indicating that this cell line expresses taurine transporter protein on the cell surface. THP1, a human promonocyte cell line, also showed a similar property. The $[^{3}H]taurine$ uptake rate was not influenced by the inflammatory inducing cytokines such as interleukin-1, gamma-interferon or interleukin-1+gamma-interferon, but was decreased by the PMA in the RAW 264.7 cell line. This suggests that activation of protein kinase C inhibits taurine transporter activity directly or indirectly. The inhibition of $[^{3}H]taurine$ uptake by PMA was time-dependent. Maximal inhibition occurred in one hr stimulation with PMA Increasing the treatment time beyond one h reduced the $[^{3}H]taurine$ uptake inhibition due to the depletion or inactivation of protein kinase C. The cell line also showed concentration-dependent $[^{3}H]taurine$ uptake under PMA stimulation. The phorbol-ester caused 23% inhibition at the concentration of 1 ${\mu}m$ PMA. The inhibition was significant even at a concentration as low as 10 nM PMA The reduced $[^{3}H]taurine$ uptake could be recovered by treatment with glucocorticosteroid hormone. Dexamethasone led to recover of the reduced taurine uptake induced by phorbol-ester, recovering maximally after one hr. This may suggest that macrophage cells require higher taurine concentration in a stressed state, for the secretion of glucocorticoid hormone is increased by hypothalamo-pituitary-adrenocortical (HPA) axis activation in the blood stream.

• Polymer(Korea)
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• v.36 no.2
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• pp.124-130
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• 2012

The uniform nanofibers of poly(L-lactide-$co$-${\varepsilon}$-caprolactone) (PLCL) with different contents of marine collagen (MC) were successfully prepared by electrospinning method. The effects of the major parameters in electrospinning process such as tip to target distance (TTD), voltage, nozzle size and flow rate on the average diameter of the electrospun nanofiber were investigated in generating composite nanofiber. The diameter and morphology of the nanofibers were confirmed by a scanning electron microscopy (SEM). Also, we measured a water contact angle to determine the surface wettability of the nanofibers. The average diameter of the nanofibers decreased as the value of TTD, MC contents, and voltages increased in comparison with that of pristine PLCL nanofiber. In contrast, the diameter of the nanofibers increased as the flow rate and inner diameter of nozzle increased in comparison with that of pristine PLCL. In addition, the hydrophilicity of the nanofiber and attachment of MG-63 cells on the sheets increased as incorporated collagen contents increased. Therefore, the marine collagen would be a potential material to enhance cellular interactivity of synthetic materials by mimicking the natural tissue.

• Journal of Navigation and Port Research
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• v.39 no.6
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• pp.465-472
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• 2015

SEEMP (Ship Energy Efficiency Management Plan) guidelines for a ship's GHG reduction include a machinery modification of hull, an installation of energy efficiency enhanced attachment in hardware methods. It is also possible to bring a ship energy efficiency improvement by fuel-efficient operations or in other software methods. Hardware modification or installation on ship can bring financial burdens to a ship company compared to its improvement expectation. On the other hand, Software based energy-saving technology can be applicable on various ship types, and it is also expected high efficiency of ship energy use compared to hardware based technology in perspective of the investment costs and efficiency. In this paper, it is described that the ship handling simulator based evaluation was carried out using representative ship model of bulk, container and VLCC. Simulation environments were separated into 6 conditions according to the sea-state and weather condition, and the operation results were compared with those before and after energy saving system applied The container ship showed the largest FOC save rate after energy saving system applied although the others also showed energy save rate after using the system.

• Asia-pacific Journal of Multimedia Services Convergent with Art, Humanities, and Sociology
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• v.8 no.6
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• pp.957-966
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• 2018

Recently, the demand for remote patient monitoring based on IoT has been increased due to aging population and an increase in single-person household. A non-contact biological signal measurement system using multiple IR-UWB radars for remote patient monitoring is proposed in this paper. To reduce error signals, a multilayer Subtraction algorithm is applied because when the background subtraction algorithm was applied to the biological signal processing, errors occurred such as voltage noise and staircase phenomenon. Therefore, a multilayer background subtraction algorithm is applied to reduce error occurrence. The multilayer background subtraction algorithm extracts the signal by calculating the amount of change between the previous clutter and the current clutter. In this study, the SVD algorithm is used. We applied the improved multilayer background subtraction algorithm to biological signal measurement and computed the respiration rate through Fast Fourier Transform (FFT). To verify the proposed system using IR-UWB radars and multilayer background subtraction algorithm, the respiration rate was measured. The validity of this study was verified by obtaining a precision of 97.36% as a result of a control experiment with Neulog's attachment type breathing apparatus. The implemented algorithm improves the inconvenience of the existing contact wearable method.

• Journal of Animal Science and Technology
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• v.54 no.4
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• pp.275-282
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• 2012

The data including milk yields, fat and protein percent for 628,395 heads collected by National Agricultural Cooperative Federation, 15 type traits and final score for 62,262 heads collected by Korea Animal Improvement Association, which were born in 1998 to 2004, and net profits calculated from milk price and raising expenses of individuals were used to estimate genetic parameters. The highest positive genetic correlation, 0.81, was shown between body depth (BD) and loin strength (SR). Genetic correlations between body depth (BD) and udder depth (UD), front teat placement (TP) and front teat length (TL) were -0.23, which were lowest among the linear type traits. Furthermore, medium level of negative genetic correlations were shown the milk yield with milk contents rate traits. Mostly low level of positive genetic correlations were shown between the milk traits and linear score traits except milk yield and stature. Most of the genetic correlations of between the linear score traits and net profit were low level of positive or negative genetic correlations. Among the genetic correlations, body depth (BD), angularity (DF) and rear attachment width (UW), and final score (FS) with net profit were high as 0.17, 0.17, 0.18 and 0.18, respectively. Finally all of the genetic correlations between net profit and milk traits were positive and higher than the linear traits with positive genetic correlations. The results of this study suggest that net profit has been related with the linear traits, such as body depth (BD), angularity (DF) and rear attachment width (UW) traits, and furthermore, milk traits including yield and contents rates influence positively and greatly on net profit.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.54 no.1
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• pp.12-24
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• 2018

This study was conducted in order to improve opening efficiency of the miniaturized large-scale net for anchovy boat seine gear to reduce the fleet size. Field experiments were performed to observe geometry of nets by catcher boats. When the distances between the two ships were 150, 300 and 450 m, and the speeds of towing nets were 0.6, 0.9, and 1.2 k't, respectively. The vertical opening and actual opening of each part of the miniaturized large-scale net was as follows: the front part of the wing net, 8.7-13.3 m, 51-78%; the middle part of the wing net, 28.1-34.2 m, 55-67%; the entrance of the inside wing net, 31.3-38.5 m, 60-73%; the square and bosom, 22.7-29.6 m, 47-62%; the entrance of the body net, 20.9-26.4 m, 42-52%; the entrance of the bag net, 17.2-21 m, 72-89%; the flapper, 13.2-15.3 m, 78-83%; and the end of the bag net, 13.2-15.7 m, 72-75%. By connecting the net pendants with the front part of the wing net, the opening of the front part of the wing net was significantly improved compared to the traditional gear, which ensured both the wing net and the inside wing net with a normal net height. This, in turn, increased the efficiency of herding. The height of the body and bag nets was also higher than that of the tradition gear. In particular, the body net attached to the gear significantly improved the pocket shape of the gear and reduced the number of fish that were caught and escaped from the bag net, which increased the rate of fishing. The tension of towing nets was measured approximately between 2,958 and 7,110 kg, which indicates that the fleet can tow nets with 350 ps, the standard engine horse power. The fishing operation time was shortened compared with of the existent net, and the large-scale buoy attachment operation was also possible to operate the ship without fish detecting boat.

• Journal of the Korean Ceramic Society
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• v.42 no.1
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• pp.56-61
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• 2005

Highly durable photocatalytic paper containing anatase $TiO_{2}$, active carbon and ceramic fiber, which can adsorb VOCs and decompose them by photo oxidation simultaneously, was manufactured and characterized. Optimum concentration of PDADMAC to let $TiO_{2}$ adhere on the surfaces of active carbon and ceramic fiber selectively was $10\~15$ ppm in a slurry mixture for making photocatalytic paper. The thickness and basis weight of the produced catalytic paper by paper-making method were 0.4 mm and 380 $g/m^{2}$, respectively. Adsorption reaction by active carbon and photocatalytic decomposition reaction by $TiO_{2}$ were proceeded simultaneously, by which the abatement rate was found to be greatly enhanced compared to the similar environment with single adsorption reaction or single photocatalytic reaction only. The selective attachment of $TiO_{2}$ on ceramic fiber and active carbon was found to be very effective in preventing decomposition of substrate by the $TiO_{2}$ attack during exposure to UV light.

• Microbiology and Biotechnology Letters
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• v.39 no.2
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• pp.140-145
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• 2011

Porcine epidemic diarrhea virus (PEDV) infection causes acute enteritis with lethal watery diarrhea resulting in a high mortality rate in piglets. As with the other members of group 1 coronaviruses, PEDV also utilizes the host aminopeptidase N (APN) as the major cellular receptor for entry into target cells. The coronavirus spike (S) protein is known to interact with the cellular surface for viral attachment and the S1 domain of all characterized coronaviruses contains a receptor-binding domain (RBD) that mediates a specific high-affinity interaction with their respective cellular receptors. Although the RBDs of several coronaviruses have been mapped, the location of the PEDV RBD has to date not been defined. As a first step toward the identification of the region of the S protein of the PEDV that is critical for recognition with the cellular receptor, we generated a series of S1-truncated variants and examined their abilities to bind to the porcine APN (pAPN) receptor. Our data indicate that the N-terminus of the S1 domain is required for pAPN association. The results from the present study may assist in our understanding of the molecular interactions between the PEDV S protein and the pAPN receptor.

• Journal of Aquaculture
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• v.3 no.1
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• pp.79-88
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• 1990

In order to obtain the basic data fir the effective seed production of sea squirt, Halocynthia roretzi(Drasche), the influence of water temperature and light intensity on spawning induction, egg development and seed settling were investigated during the Period from December 24, 1906 to February 24, 1987. The maximum number of eggs spawned was obtained at $14^{\circ}C$. The relationships between the water temperature (T, $^{\circ}C$) and the required time (h, hour) in each egg developmental stage were given as follows : Up to 8 cell 1/h =0.0147 T-0.0069(.=0.9816), Up to 32 cell 1/h =0.0100 T-0.0017(r=0.9672), Up to early tadpole 1/h =0.0043 T+0.0024(r=0.9913), Up to hatching larva 1/h =0.0021 T-0.0021 (r=0.9898). The highest rate of seed attachment was obtained at $14^{\circ}C$ of water temperature.

• Journal of the Korean Society of Food Science and Nutrition
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• v.34 no.4
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• pp.489-495
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• 2005

The purpose of this study was to analyze the effect of the consumer-brand relationship Quality (CBRQ) on the brand loyalty in the fast food restaurant in Masan, Korea. The Questionnaires were distributed to 250 students in the K University located in Masan, who were sampled by convenience-sampling method. The surveys were performed from December 6 to 14, 2004. The 246 Questionnaires were responded, and 12 unusable Questionnaires were excluded, then 234 were used for the final analysis $(response rate:\;93.7\%)$. For the statistical analysis, SPSS (12.0) was used to conduct the descriptive analysis, factor analysis, reliability analysis, Pearson correlation and multiple regression analysis. The result of this study showed that 2 constructs (satisfaction and commitment) of consumer-brand relationship Quality have significant positive effects on the brand loyalty in the fast food restaurant. It meant that as consumer-brand relationship quality becomes stronger, the customer's brand loyalty becomes greater. The differences of customer loyalty between the high and low group of self-connective attachment, satisfaction, commitment, trust and intimacy were significant. So the brand loyalty of high group of customer-brand relationship quality was significantly higher than that of low group of customer-brand relationship quality. As a conclusion, the operator in the fast food restaurant should focus on the marketing strategy to strengthen the quality of consumer-brand relationship in order to increase the brand loyalty of customers.