• Microbiology and Biotechnology Letters
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• v.6 no.4
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• pp.167-171
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• 1978

When Asp. niger was shaked at 3$0^{\circ}C$ in 500 mι Erlenmeyer flask with 50 ml of the medium containing 14% of Korean local commercial glucose brand, 0.45% of peptone, and mineral, the citric acid was produced at the level of 37~43 gram per liter in 8 days, and the citric acid production at medium containing X glucose brand was better than that containing Y glucose brand. When the contaminated minerals were removed from the local glucose by Ambelite-IR 120 and peptone by potassium ferrocyanide followed by readjustment of ferric ion content in the medium to 10 mg per liter, the citric acid formation reached 53 gram per liter, a production level of three times higher than that with the original Sakaguchi's medium. The further physiological studies and the mutation of isolated Asp. niger will be needed.

• Bulletin of the Korean Chemical Society
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• v.32 no.2
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• pp.590-594
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• 2011

An aminocarboxy aspartic acid-bonded silica (Asp-Silica) stationary phase was synthesized using L-aspartic acid as ligand and silica gel as matrix. The standard protein mixtures were separated with prepared chromatographic column. The effects of solution pH, salt concentration and metal ion on the retention of proteins were examined, and also compared with traditional iminodiacetic acid-bonded silica (IDA-Silica) column. The results show that Asp-Silica column exhibited an excellent separation performance for proteins. The retention of proteins on Asp-Silica stationary phase was consistent with electrostatic characteristic of cation-exchange. The stationary phase displayed typical metal chelate property after fixing copper ion (II) on Asp-Silica. Under competitive eluting condition, protein mixtures were effectively isolated. Asp ligand showed better ion-exchange and metal chelating properties as compared with IDA ligand.

• Journal of the Korean Society of Food Science and Nutrition
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• v.28 no.1
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• pp.61-66
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• 1999

The purpose of this study was to improve the palatability of kochujang. The activities of liquefying amylase and saccharogenic amylase in Monacus anka koji were lower than those in Asp. oryzae koji. The acid protease activity(1.4 units/g) in M. anka koji was also lower than that(1.6 units/g) in Asp. oryzae koji. Glucosamine amount in Asp. oryzae koji was 1075 g, and that in M. anka koji was 318 g. Four kinds of Kochujang were prepared with Asp. oryzae koji(A), M. anka koji(M), mixed koji of Asp. oryzae and M. anka(A＋M), and mixture of M. anka koji and malt(M＋M). The pH of four kinds of kochujang was 5.1 in the beginning and was between 4.70~4.83 after 120 days of fermentation. Reducing sugar of kochujang was between 16.3~20.7% after fermentation and kochujang prepared with M＋M showed the highest reducing sugar content. Amino nitrogen of kochujang was between 182 mg%~230 mg% after fermentation and the highest amino nitrogen content was observed at kochujang prepared with A＋M. Kochujang prepared with M and M＋M showed higher a value than kochujang prepared with A and A＋M.

• Journal of Aquaculture
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• v.21 no.1
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• pp.54-59
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• 2008

The present study aimed to find the best diluent and cryoprotectant for sperm cryopreservation of filefish Thamnaconus modestus. Two kinds of artificial seminal plasma(ASP1, ASP2), 0.3 M glucose and marine fish Ringer's solution(MFRS) were employed as diluent. Dimethyl sulfoxide(DMSO) and methanol as cryoprotectant were selected for sperm cryopreservation. Sperm was diluted at the ratio of 1:3 with diluents containing cryoprotectants and adjusted for final concentration at 5%, 10%, 15% and 20%. Mixed milt was frozen at liquid nitrogen vapor after equilibration for 5 min. The highest motility($40.5{\pm}2.8%$) and swimming speed($81.5{\pm}4.1{\mu}m/s$) of frozen/thawed sperm were observed in ASP1 diluent containing 10% DMSO and in ASP2 containing 15% DMSO, respectively. Results showed that cryopreservation with ASP as diluent and DMSO as cryoprotectant could be adopted for long term storage of filefish sperm.

• Korean Journal of Food Science and Technology
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• v.14 no.1
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• pp.72-79
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• 1982

Several mutants were isolated from the parent strain of Aspergillus niger CF: the first mutant strain CF-11 was obtained by UV irradiation, and the second mutant strain CF-21 and CF-22 were from NTG (N-methyl-N'-nitroso-N-nitroso-guanidine) treatment on the CF-11. These mutants were characterized, and their enzyme and acid production on wheat bran Koji and wheat flour Koji were studied. Asp. niger CF-22 mutant appeared to be tan type which conidial heads were discolored. It's glucoamylase activity was inreased approximately two times and its ${\alpha}-amylase$ about 50 percent as compared with that of the parent strain of Asp. niger CF, when grown on wheat bran Koji under the optimal conditions. Asp. niger CF-21 mutant showed slower growth rate and poor sporulation than the wild type, although its conidial heads were not discolored. Approximately 4-fold increment in its acid production was observed as compared with the weld type. The activities of glucoamylase and ${\alpha}-amylase$ of the Asp. niger CF-22 and CF-21 mutants were higher than those of the wild type, but their protease activity was rather lower. The maximum production of glucoamylase by the Asp. niger CF-22 mutant was obtained after 2 to 3 days incubation on wheat bran at 30 to $35^{\circ}C;$ ${\alpha}-amylase$2 days incubation at 30 to $35^{\circ}C$. The maximal levels of acid production by the mutant CF-21 was appeared after 2 days incubation on wheat bran Koji, and after 3 days on wheat flour Koji at $30^{\circ}C$. Little differences in the levels of acid production were observed between on wheat bran and flour Koji.

• Proceedings of the CALSEC Conference
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• 2003.09a
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• pp.313-318
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• 2003

■ 인터넷 전자상거래의 성장에 따라 e-Commerce 인프라 서비스로서 물류에 대한 시장수요의 급격한 증대 ㆍ인터넷 전자상거래, 특히 B2C 인터넷 쇼핑목의 성장에 따른 사업기회를 포착하기 위하여 EC 사업자와 연계하여 운영할 수 있는 ASP 방식의 퀵서비스 Gateway에 대한 개발 필요성이 높아지고 있음. ■ 소화물택배 시장의 성장과 함께 퀵 서비스에 대한 기업 및 개인 고객의 수요 역시 지속적인 성장 추세를 보이고 있음 ㆍ 오프라인의 기업고객 및 개인고객의 수요에 부응할 수 있는 고품질의 서비스를 안정적으로 제공하기 위한 측면에서도 "퀵서비스 ASP"사이트 구축에 대한 필요성이 높아지고 있음(중략)

• Proceedings of the Korea Association of Information Systems Conference
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• 2005.12a
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• pp.443-454
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• 2005

The purpose of this study is to show the operations and implementations of ASP web database which uses open-source web site packages called bighitonline video shop. Some literature reviews have been done to reveal robust theoretical background for this research And also, some basic experiments as Preparatory Processes have also been made by using some softwares such as Windows XP professional, IIS, MS SQL Server and others. The main purpose of these experiments is to show strong theoretical and practical backgrounds. These experimental results in the micro-level showed to be successful to understand the Interrelations of web components and web-site architectures.

• Proceedings of the KAIS Fall Conference
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• 2001.11a
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• pp.240-244
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• 2001

인터넷의 등장과 웹의 발달로 인간은 사이버세계에서 활동을 할 수 있게 되었고 수많은 청소년들에겐 새로운 호기심을 유발하게 되었다. 이에 발맞추어 청소년의 고민을 해결할 수 있는 곳으로 웹이 이용되었으며 사이버상담이 등장하였다. 여러 대학교에서도 이러한 사항을 고려하여 상담시스템을 구축하고 있다. 교사와 학생들이 이용할 수 있도록 본 논문은 청소년의 상담시스템을 설계 및 구현하기 위해 기존의 정적인 HTML페이지와는 다른 동적인 ASP페이지를 이용하여 사이버 카운슬링을 구축하였다.

• Korean Journal of Microbiology
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• v.18 no.1
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• pp.1-6
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• 1980

Five species of food decay fungl, Aspergillus flavus, Asp. uiger, Penicillum sp., Botrytis cinerea, and Rhizopus stolonifer, were examined for their radio sensitivity in several suspension media. Asp. flavus, Asp. niger and Penicillum sp. have almost the same sensitivity toward gamma rays. with D value in the range of 30 to 35 K rad, whereas Botrytis cinerea has a D value of approximately 55K rad and Rhizopus stolonifer, the most re4sistant fungus studied, has a crease in their radioresistnace when compared with spores irradiated in water. Asp. flavus and penicillium sp. spores irradiated in citrate buffer at pH3-7 showed almost no change in their radisensitiity with pH, but Botryis cinerea spores showed a distinct decrease in their radioresistnace at pH 6 and 7. Penicillum sp. spores irradiated in sucrose solutions showed no sinificant change in their radioresistance. Botrytis cinerea spores displayed a higher radioresistance when they were irradiated in sucrose solution than in water.

• Applied Biological Chemistry
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• v.25 no.4
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• pp.252-256
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• 1982

To investigate the influences of biological factors on stored rice and their interactions, the activities of red Hour beetle(Tribolium castaneum) and 2 species of Aspergillus was observed during 6 weeks at $28^{\circ}C{\pm}1^{\circ}C$. It was found that the red flour beetle could complete its life cycle on rice in six weeks. Its activity caused the increse of moisture content of rice(max. 1.3%) after 6 weeks, but the number of mold propagule on the surface of stored rice decreased. The growth of red flour beetle showed a stabilizing tendency on rice inoculated with Asp. repens while it was retarded with Asp. niger.