• 생물환경조절학회지
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• 제2권1호
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• pp.37-45
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• 1993

국내 원예시설이 고정화되면서 각종 연작 장해가 나타남에 따라 객토와 토양소독의 필요성이 커지고 있으나 객토와 토양소독에 소요되는 비용 증가가 큰 문제로 대두되고 있다. 이에 따른 간이한 양액재배 시스템으로서 농가에서도 투자비용과 운용비용이 저렴하면서 환경오염의 염려가 없거나 적은 각종 인공배지를 활용한 토마토 자루재배(bag culture) 실험결과는 다음과 같다. 1. 단용배지를 이용한 토마토 자루배재에서 질석, 훈탄, 모래, 페놀수지 (PRE), 입상면, perlite 등에서 생육, 수량 및 품질면에서 이용가능성이 높고 이들 배지의 단점을 보완한다면 암면 대체도 가능할 것으로 보인다. 2. 혼합배지를 이용한 토마토 자루재배에서 perlite와 입상면 혼합처리에서는 perlite 20-40％수준과 입상면 80-60％수준에서, peatmoss와 질석 혼합처리에서는 peatmoss 40-60％와, 질석 60-40％ 혼합비율에서, peatmoss+질석 +입 상면 혼합처리에서는 peatmoss를 50-60％로 하고 질석과 입상면을 각각 25-20％로 혼합한 경우에서 각각 높은 수량과 품질을 보여주었다.

• 한국균학회지
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• 제17권3호
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• pp.124-131
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• 1989

소나무톱밥을 이용(利用)한 버들송이 버섯의 톱밥인공재배(人工栽培)를 위한 균사배양조건(菌絲培養條件)과 톱밥배지(培地) 제조시(製造時) 첨가제(添加劑) 종류(種類)와 혼합비율(混合比率)에 따른 인공재배(人工栽培)의 가능성(可能性)을 검토한 결과는 다음과 같다. 1. 버들송이 버섯의 균사배양(菌絲培養) 최적온도(最適溫度)는 $25^{\circ}C$, pH는 4.0, 균사배양일수(菌絲培養日數)는 18일(日)에서 균사생장(菌絲生長)이 가장 좋았다. 균주별(菌株別) 균사체량(菌絲體量)은 ASI 19002 균주(菌株)가 980mg/18일로 가장 많았고 다음은 ASI 19003, 19004 순이었다. 2. 소나무톱밥을 이용(利用)한 인공재배시(人工栽培時) 적합한 균주(菌株)는 ASI 19003 균주(菌株)로서 균사밀도(菌絲密度)가 가장 양호(良好)하였고, 자실체(子實體) 수량(收量)은 ASI 19003 균주(菌株)가 미강(米糠) 10%에서 72g/병, ASI 19004 균주(菌株)는 미강(米糠) 30%에서 72g/병으로 가장 많았으나 ASI 19004 균주(菌株)는 품질(品質)이 불량(不良)하였다. 3. 소나무톱밥 인공재배시(人工栽培時) 첨가제(添加劑) 종류(種類)와 혼합비율(混合比率)에 따른 균사생장(菌絲生長)은 밀기울 20% 첨가구(添加區)에서 65 mm/23일로 타첨가제(他添加劑)보다 가장 빨랐으며 , 첨가제(添加齋)를 첨가(添加)한 구에서는 자실체(子實體)가 형성(形成)되는 반면에 소나무톱밥 단 용구에서는 자실체(子實體)가 형성(形成)되지 않았다. 4. 버들송이 인공배지(人工培地)의 최적수분함량(最適水分含量)은 $65{\sim}75%$ 범위였으며, 종균배양(種菌培養) 완성일(完成日)은 65%일때 23일(日)로 가장 빨랐고, 초발용소요일수(初發茸所要日數)는 60% 이상에서 $9{\pm}1$일(日) 이었으며, 자실체(子實體) 수량(收量)은 65%에서 $52.3g{\pm}0.6$으로 편차가 적었다. 5. 소나무톱밥에 첨가종류(添加種類) 및 혼합수준별(水準別) 자실체(子實體) 수량(收量)은 밀기울 30% 첨가구(添加區)에서 유효경수가 21.3개/병, 버들송이 자실체(子實體) 수량(收量)은 107g/병으로 가장 많았고 초발용(初發茸) 소요일수(所要日數)도 8일(日)로 빨랐다.

• 한국환경과학회지
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• 제19권9호
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• pp.1137-1142
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• 2010

This study proposes a guideline of a green roof system suitable for the local environment by verifying the growth of Zoysia japonica in a shallow, extensive, green roof system under rainfed condition. The experimental soil substrates into which excellent drought tolerance and creeping Z. japonica was planted were made with different soil thicknesses(15cm, 25cm) and soil mixing ratios(SL, $P_7P_1L_2$, $P_6P_2L_2$, $P_5P_3L_2$, $P_4P_4L_2$). The plant height, green coverage ratio, fresh weight, dry weight and chlorophyll contents of Z. japonica were investigated. For the soil thickness of 15cm, the plant height of Z. japonica was significantly as affected by the soil mixing ratio and it was shown in the order SL= $P_4P_4L_2$ < $P_7P_1L_2$ = $P_5P_3L_2$ < $P_6P_2L_2$. For the soil thickness of 25cm, the plant height was increased in order to SL < $P_7P_1L_2$, $P_6P_2L_2$, $P_5P_3L_2$ < $P_4P_4L_2$. The green coverage ratio was not observed by soil the mixing ratio or soil thickness. However, the green coverage ratio was 86~90% with a good coverage rate overall. The chlorophyll contents of Z. japonica were not significantly affected by the soil mixing ratio in the soil thickness of 15cm, but were higher in the natural soil than in the artificial soil at 25cm soil thickness. The fresh weight and dry weight of Zoysia japonica were heavier in the 25cm thickness than in the 15cm thickness and in the artificial soil mixture than in the natural soil. The result indicated that the growth of Zoysia japonica was more effective in the 25cm soil thickness with artificial soil than in the 15cm soil thickness with natural soil in the green roof system under rainfed condition.

• 현장농수산연구지
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• 제21권2호
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• pp.35-47
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• 2019

간버섯 균주의 균사 생장은 PDA와 YMA에서 균사의 밀도와 생장량이 양호하였으나, MEA에서는 균사 생장량과 속도가 저조하였다. 배양 기간 중 광(光)조사는 균사체의 색소 형성과 균사체 밀도에 영향을 주었다. 간버섯은 병 재배와 봉지 재배 모두에서 자실체를 발생시킬 수 있었고 종균 배양과 재배를 위한 가장 적합한 배지 원료는 참나무톱밥으로 밝혀졌다. 톱밥배지를 사용한 인공재배에서 자실체의 발생은 15일째부터 육안으로 확인할 수 있는 정도로 자실체 원기가 생장하였으며 상대습도가 낮은 처리구에서 5일 정도 빠르게 형성되었다. 버섯 발생 40~45일째에는 성숙한자실체를 수확할 수 있었고, 생육실의 상대습도가 낮은 편이 버섯 발생과 생육에 유리하였다. 인공재배에서 수확한 자실체의 항산화 활성을 조사한 결과, 간버섯의 ABTS radical 소거 활성은 병 재배 자실체 추출물과 야생 간버섯 자실체 methanol 추출물이 각각 505㎍/㎖, 515㎍/㎖의 농도로 낮은 활성을 나타내었으나 봉지 재배에서 수확한 자실체는 910㎍/㎖로 높게 나타났다. DPPH radical 소거 활성 결과 모든 추출물이 활성 2,000㎍/㎖ 농도 이상의 IC₅₀값으로 활성을 나타내지 않았다. 간버섯의 ethyl acetate 추출물의 경우 간버섯 병 재배 자실체 추출물이 IC₅₀값 154.5㎍/㎖으로 가장 높은 활성을 보이는 것으로 나타났으며, 간버섯 methanol 추출물은 동일 농도(10mg/㎖)에서 ABTS radical 소거 활성은 야생 간버섯 자실체 추출물이 가장 높은 활성을 보였다.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2008년도 추계학술대회 논문집 Vol.21
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• pp.399-399
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• 2008

Synthesis and characterization of ZnO structure such as nanowires, nanorods, nanotube, nanowall, etc. have been studied to multifunctional application such as optical, nanoscale electronic and chemical devices because it has a room-temperature wide band gap of 3.37eV, large exiton binding energy(60meV) and various properties. Various synthesis methods including chemical vapor deposition (CVD), physical vapor deposition, electrochemical deposition, micro-emulsion, and hydrothermal approach have been reported to fabricate various kinds of ZnO nanostructures. But some of these synthesis methods are expensive and difficult of mass production. Wet chemical method has several advantage such as simple process, mass production, low temperature process, and low cost. In the present work, ZnO nanorods are deposited on ITO/glass substrate by simple wet chemical method. The process is perfomed by two steps. One-step is deposition of ZnO seeds and two-step is growth of ZnO nanorods on substrates. In order to form ZnO seeds on substrates, mixture solution of Zn acetate and Methanol was prepared.(one-step) Seed layers were deposited for control of morpholgy of ZnO seed layers by spin coating process because ZnO seeds is deposited uniformly by centrifugal force of spin coating. The seed-deposited samples were pre-annealed for 30min at $180^{\circ}C$ to enhance adhesion and crystallinnity of ZnO seed layer on substrate. Vertically well-aligned ZnO nanorods were grown by the "dipping-and-holding" process of the substrates into the mixture solution consisting of the mixture solution of DI water, Zinc nitrate and hexamethylenetetramine for 4 hours at $90^{\circ}C$.(two-step) It was found that density and morphology of ZnO nanorods were controlled by manipulation of ZnO seeds through rpm of spin coating. The morphology, crystallinity, optical properties of the grown ZnO nanostructures were carried out by field-emission scanning electron microscopy, high-resolution electron microscopy, photoluminescence, respectively. We are convinced that this method is complementing problems of main techniques of existing reports.

• Journal of Ecology and Environment
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• 제38권3호
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• pp.375-381
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• 2015

We investigated the influence of summer rainfall on Pectinatella magnifica colonies in lotic ecosystems. Of the examined substrate materials, branches and aquatic macrophytes supported more colonies of P. magnifica than that by stones or artificial materials. The influence of rainfall on P. magnifica colonies differed in accordance with the type of substrate material at each study site. In the Geum River, little difference was noted in the number of P. magnifica colonies on branches before ($mean{\pm}SE$, $24{\pm}7.3$ individuals) and after rainfall ($20{\pm}8.4$ ind.); other substrate types supported fewer colonies of P. magnifica after rainfall. In contrast, in the Miryang River, rainfall had minimal effect on the number of P. magnifica colonies supported by macrophytes ($13{\pm}3.8$ and $12{\pm}4.3$ ind., respectively). Artificial material was more abundant in the Banbyeon Stream where it was able to support more colonies of P. magnifica. We found that the structure of different substrates sustains P. magnifica following rainfall. In the Miryang River, free-floating and submerged plants with a relatively heterogeneous substrate surface were the dominant macrophytes, whereas in the Geum River, simple macrophytes (i.e., emergent plants) were dominant. Therefore, we conclude that the substrate type on which P. magnifica grows plays an important role in resisting physical disturbances such as rainfall.

• 상하수도학회지
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• 제18권4호
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• pp.515-521
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• 2004

Anaerobic digestion has many advantages over the more conventional aerobic treatment processes such as low levels of excess sludge production, low space (area) requirements, and the production of valuable biogas. The purpose of this study was to evaluate the effect of organic loading rate of anaerobic digestion on thermophilic($55^{\circ}C$) and mesophilic($35^{\circ}C$) conditions. Fluorescent in situ hybridization (FISH) method was also used to study the microbial community in the reactors. The stabilizing time in mesophilic anaerobic reactors was shorter as approximately 20 days than 40 days in the thermophilic anaerobic reactors. The amount of methane production rate in anaerobic reactors was independent of the concentrations of supplied substrates and the amount of methanogens. When the microbial diversity in the mesophilic and thermophilic reactors, which had been treated with acetate-based artificial wastewater, were compared, it was found that methanogenesis was carried out by microbial consortia consisting of bacteria and archaea such as methanogens. To investigate the activity of bacterial and archaeal populations in all anaerobic reactors, the amount of acetate was measured. Archaea were predominant in all reactors. Interestingly, Methanothrix-like methanogens appeared in mesophilic anaerobic reactors with high feed substrate concentrations, whereas it was not observed in thermophilic anaerobic reactors.

• 환경생물
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• 제35권1호
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• pp.1-5
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• 2017

A native species to the European ocean, the tunicate Ciona intestinalis now appears worldwide, from sub-Arctic to tropical regions. C. intestinalis generally occurs as an opportunistic fouling organism on artificial substrates in harbors or aquaculture. This study focused on estimating the early growth pattern of C. intestinalis under various temperature conditions. Adults were collected from the Guryungpo harbour in November 2016, and their artificially inseminated eggs were used for the study. The growth of the C. intestinalis was investigated at 8 constant temperatures, ranging from $12^{\circ}C$ to $26^{\circ}C$, at 30 psu. Results indicate that the growth of C. intestinalis increased with increasing temperature. The growth was sluggish at $12^{\circ}C$, and decreased at $26^{\circ}C$. The optimal temperature for growth of C. intestinalis was therefore estimated to be between $20^{\circ}C$ and $24^{\circ}C$.

• Journal of Microbiology and Biotechnology
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• 제20권4호
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• pp.737-748
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• 2010

Glutaminase production in Zygosaccharomyces rouxii by solid-state fermentation (SSF) is detailed. Substrates screening showed best results with oatmeal (OM) and wheatbran (WB). Furthermore, a 1:1 combination of OM:WB gave 0.614 units/gds with artificial sea water as a moistening agent. Evaluation of additional carbon, nitrogen, amino acids, and minerals supplementation was done. A central composite design was employed to investigate the effects of four variables (viz., moisture content, glucose, corn steep liquor, and glutamine) on production. A 4-fold increase in enzyme production was obtained. Studies were undertaken to analyze the time-course model, the microbial growth, and nutrient utilization during SSF. A logistic equation ($R^2$=0.8973), describing the growth model of Z. rouxii, was obtained with maximum values of ${\mu}_m$ and $X_m$ at $0.326h^{-1}$ and 7.35% of dry matter weight loss, respectively. A goodfit model to describe utilization of total carbohydrate ($R^2$=0.9906) and nitrogen concentration ($R^2$=0.9869) with time was obtained. The model was used successfully to predict enzyme production ($R^2$=0.7950).

• Journal of Microbiology and Biotechnology
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• 제14권3호
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• pp.635-638
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• 2004

An ATP-binding-cassette (ABC) transporter gene in the cephabacin biosynthetic gene cluster of Lysobacter lactamgenus was characterized. The amplified orf10 (cpbJ) gene was subcloned into pET-28a(+) vector and expressed in E. coli BL21(DE3) strain by 0.5 mM IPTG at $30^{\circ}C$. The membrane fraction of recombinant E. coli cells was separated by ultracentrifugation, and solubilized using 2.5% octyl-$\beta$-D-glucoside. Using the solubilized membrane fraction, the artificial proteoliposomes were reconstituted and analyzed for the biological activity of CpbJ protein. Upon measuring ATPase activity, the proteoliposome made from recombinant E. coli membrane proteins showed slightly higher activity than that from host E. coli membrane proteins. In the measurement of membrane transport activity, the reconstituted proteoliposome of recombinant E. coli membrane proteins exhibited higher activity when both substrates of cephalosporin C and L-Ala-L-Ser were applied, compared to the case of cephalosporin C or L-Ala-L-Ser only. It implies that the CpbJ protein is an ABC transporter secreting cephabacin antibiotics synthesized in cytoplasm.