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In situ Analysis of Methanogenic Bacteria in the Anaerobic Mesophilic and Thermophilic Sludge Digestion  

Hwang, Sun-Jin (경희대학교 환경응용화학대학 및 환경연구센타)
Jang, Hyun-Sup (경희대학교 환경응용화학대학 및 환경연구센타)
Eom, Hyoung-Choon (한국지질자원연구원)
Jang, Kwang-Un (경희대학교 환경응용화학대학 및 환경연구센타)
Publication Information
Journal of Korean Society of Water and Wastewater / v.18, no.4, 2004 , pp. 515-521 More about this Journal
Abstract
Anaerobic digestion has many advantages over the more conventional aerobic treatment processes such as low levels of excess sludge production, low space (area) requirements, and the production of valuable biogas. The purpose of this study was to evaluate the effect of organic loading rate of anaerobic digestion on thermophilic($55^{\circ}C$) and mesophilic($35^{\circ}C$) conditions. Fluorescent in situ hybridization (FISH) method was also used to study the microbial community in the reactors. The stabilizing time in mesophilic anaerobic reactors was shorter as approximately 20 days than 40 days in the thermophilic anaerobic reactors. The amount of methane production rate in anaerobic reactors was independent of the concentrations of supplied substrates and the amount of methanogens. When the microbial diversity in the mesophilic and thermophilic reactors, which had been treated with acetate-based artificial wastewater, were compared, it was found that methanogenesis was carried out by microbial consortia consisting of bacteria and archaea such as methanogens. To investigate the activity of bacterial and archaeal populations in all anaerobic reactors, the amount of acetate was measured. Archaea were predominant in all reactors. Interestingly, Methanothrix-like methanogens appeared in mesophilic anaerobic reactors with high feed substrate concentrations, whereas it was not observed in thermophilic anaerobic reactors.
Keywords
anaerobic digestion; methanogens; archaea; FlSH;
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1 Sekiguchi, Y., Y. Kamagata, K. Syutsubo, A. Ohashi, H. Harada, and K. Nakamura (1998) Phylogenetic diversity of mesophilic and thermophilic granular sludges determined by 16S rRNA gene analysis, Microbiowgy 144, 2655-2665
2 Amann, R. I. (1995) In situ identification of micro-organisrns by wholecell hybridization with rRNA-targeted nucleic acid probes, section 3.3.6, p. 3.3.6/1-3.3.6/15. In A. D. L. Akkermans, and J. D. van Elsas (ed.), Molecular microbial ecology mnnual. Kluwer, Academic Publishers, London, England
3 황선진. Keisuke Hanaki (1999) 질소화합물올 함유한 고형폐기물로부터의 아산화질소 ($N_{2}O$) 발생특성 및 FISH법에 의한 미생물군집의 해석에 관한 연구, 한국폐기물학회지. 16(3). pp.271-279
4 장덕, 정태학 (1988) 협기성소화의 동력학: 중온 및 고온협 기성소화의 비교연구, 대한토목학회논문집. 8(4), pp. 59-67
5 Van Lier, J. B., K C. F. Grolle, A.J.M. Stams, E. Conway de Macario, and G. Lettinga (1992) Start-up of a thermophilic upflow anaerobic sludge bed (UASB) reactor with mesophilic granularsludge. Appl. Microbiol. Biotechnol. 37, 130-135
6 Alm, E. W., D. B, Oerther, N. Larsen, D.A. Stahl, and L. Raskin(1996) The oligonucleotide probe database. Appl. Environ. Microbiol. 62, 3557-3559