• Journal of Mushroom
• /
• v.2 no.4
• /
• pp.207-213
• /
• 2004

The results of examining characteristics of mycelial growth and culture condition for determining the condition of artificially culturing Fomitopsis pinicola are as follows. 1) Mycelial growth and density of F. pinicola. were the highest in the medium of PIDA(Pine Dextrose Agar;66.3mm/10d) followed by the order of GDA, PDA, CDA, PODA, ODA, YM, MCM, MEA(pH 4.7), CHA, and MEA(pH 4.7). 2) Optimal temperature for the mycelial growth and density of F. pinicola were shown to be $30^{\circ}C$, but the mycelia were dead at $40^{\circ}C$. the mycelial growth and density of KNAC9005 strains was the highest at $30^{\circ}C$(66.3mm/10d) followed by the order of 25, 20, 15, 35, 10, and $5^{\circ}C$. 3) Optimal pH for the mycelial growth and density of $40^{\circ}C$ was revealed to be 6.0(88.4mm/10d). above or below pH 6.0, the mycelial growth and density were shown to be retarded. 4) Optimal carbon, nitrogen and organic acid sources for the spawn growth of $40^{\circ}C$ were maltose(331mg/25ml/15d), peptone(347mg/25ml/15d), and glutamic acid(357mg/25ml/15d), respectively. Optimal level of biotin was 370mg/15d and optimal C/N ratio was 40.

• Korean Journal of Plant Tissue Culture
• /
• v.22 no.5
• /
• pp.261-266
• /
• 1995

This study was carried out to develop new varieties which are dwarf and tolerant to winter cold in the Aurantioideae by intergeneric crossing. to do that, the reciprocal crosses of Hwanggeumyooza and trifoliate orange, yooza and trifoliate orange were done and in vitro immature ovule culture of their hybrid was carried out .The callus formation from immature ovule was good in order of Hwanggeumyooza, Hwanggeumyooza $\times$ tifoliate orange, yooza, and trifoliate orange and best at 1 to 3 mg/L NAA+0.5mg/L zeatin on MT medium. In vitro germination percentage of 20week old hybrid of Hwanggeumyooza $\times$ tifoliate orange and trifoliate orange $\times$ Hwanggeumyooza were 41.3% and 37.7, respectively. The phenotype of hybrid (95%) of Hwanggeumyooza $\times$ trifoliate orange and that (100%) of trifoliate orange $\times$ Hwanggeumyooza were similar to that of trifoliate orange. After Hwanggeumyooza was pollinated by pollens of trifoliate orange, the pollen tubes grew on stigma after 3h of pollination and entered into micropyle after about 24~28 h. One gamete in pollen was fused with polar nuclei after 2 days and other one fused with egg nucleus at 3days after pollination. The fruit set percentage by intergeneric crossing was 14.0% in Hwanggeumyooza $\times$ trtfoliate orange and 17.5% in trifoliate orange $\times$ Hwanggeumyooza. The fruit set percentages of Hwanggeumyooza. and trifoliate orange were 34.2% and 39.5% by artificial self-fertilization, 34.2% and 39.5% by artificial cross fertilization, 3.1% and 1.4% by parthenocarpy and 13.0% and 3.0% by natural fertilization, respectively. The somatic and gametic chromosome numbers of Hwanggeumyooza, yooza, and trifoliate orange were 2n=18 and n=9.

• Korean Journal of Environmental Biology
• /
• v.18 no.1
• /
• pp.153-161
• /
• 2000

This study was carried out to examine the drift of Gammarus sobaegensis by acid stress as pH depression in Oweol creek from April 1996 to October 1996. The behavioral drifting was revealed to the characteristics of G. sobaegensis that is tend to increase as acid stress. And, tolerance level of G. sobaegensis to pH depression was different among the size classes. Individuals belong to small to medium size classes were weak in lower pH. Pattern of response in G. sobaegensis has a lower tolerance to acid stress at below pH 4.0 than above pH 5.0 in the artificial channel and show the possibility as an effective aquatic ecotoxicity test organism. The result of analysis of variance, water temperature (F-ratio : 66.596, p< 0.0005) and the size classes (F-ratio : 71.386, p< 0.0005) except pH level (F-ratio : 353.415, p<0.0005) were showed to the major factor for drift behavior by acid depression. [Gammarus, pH, Drift, Acid stress, Ecotoxicity test].

• Journal of Intelligence and Information Systems
• /
• v.20 no.4
• /
• pp.43-58
• /
• 2014

As the use of trading systems has increased rapidly, many researchers have become interested in developing effective stock market prediction models using artificial intelligence techniques. Stock market prediction involves multifaceted interactions between market-controlling factors and unknown random processes. A successful stock prediction model achieves the most accurate result from minimum input data with the least complex model. In this research, we develop a combination model of ${\pi}$-fuzzy logic and support vector machine (SVM) models, using a genetic algorithm to optimize the parameters of the SVM and ${\pi}$-fuzzy functions, as well as feature subset selection to improve the performance of stock market prediction. To evaluate the performance of our proposed model, we compare the performance of our model to other comparative models, including the logistic regression, multiple discriminant analysis, classification and regression tree, artificial neural network, SVM, and fuzzy SVM models, with the same data. The results show that our model outperforms all other comparative models in prediction accuracy as well as return on investment.

• Microbiology and Biotechnology Letters
• /
• v.29 no.2
• /
• pp.115-121
• /
• 2001

Biological Treatment of Wastewater Containing Chlorinated Phenols by a Mixed Culture. Lee, Wan-Seok1, Sang-Wook Jung, Chan-Sun Park, Byung-Dae Yoon, Jang-Eok Kim\ and Hee-Mock Oh*. Environmental Bioresources Laboratory, Korea Research Institute of Biosicence and Biotechnology, Taejon, Korea, 1 Department of Agricultural Chemistry, Kyungpool< National University, Taegu, Korea - The biodegradation of chlorinated phenols in an artificial wastewater was investigated using a mixed culture. The mixed culture was composed of 8 microorganisms isolated from the soil contaminated with various chlorinated phenols. Pseudomonas sp. BM as a main constituent of a mixed culture was Gram-negative, catalase- and oxidase-positive, and rod-shaped, and did not grow at 41°C. It degraded 99% of initial 500 mg!1 of pentachlorophenol (PCP) in the minimal salts medium as a sole source of carbon and energy within 3 days. The degradation efficiency of Pseu.domon.as sp. BM was not affected by the other organic carbon and nitrogen compounds. Pseudomonas sp. BM was able to grow in a broad range of pH 5 - 8, and degrade 2,000 mg/1 PCP. In the experiment with an artificial wastewater containing chlorinated phenols, the degradation efficiency of the mixed culture was the range of 73% (2,4-dichlorophenol) -96% (2-chlorophenol) during an incubation of 7 days. In a continuous culture experiment, the degradation efficiency of mixed culture plus activated sludge was about 2 times higher than that of the control containing only activated sludge. These results indicate that it is possible to apply the mixed culture to other wastewaters containing chlorinated phenols. Key words: Biodegradation, chlorinated phenols, pentachlorophenol, Pseudomonas sp. BM

• Journal of Conservation Science
• /
• v.31 no.4
• /
• pp.443-455
• /
• 2015

To verifying the stability of Natural pigments for Dancheong which is available on the market was carried out as light resistance and gas corrosion test. In this particular case, we will confirm the characteristics of the only pigments except of Dancheong technique and influence of substance. Medium using for coloring are animal glue and synthetic resin that widely used for Dancheong in currently. Selected to artificial light source is a xenon arc lamp, contaminated gas is nitrogen dioxide gas in experiment. Degree of degradation of pigment were compared by measuring the degree of color change. The result of light resistance test, mineral pigments were good; color is green and blue type, soil pigments also good; color is yellow ocher, white clay, red clay etc. Gamboge, unghwang, cinnabar, red lead were confirmed not good. The result of gas corrosion test, most of the natural pigments were good condition except the red lead. Generally, animal glue samples had better than a synthetic resin samples.

• Weed & Turfgrass Science
• /
• v.6 no.4
• /
• pp.306-312
• /
• 2017

This study was carried out to develop new hybrid zoysiagrass cultivar 'Seah' (The application no. for cultivar protection : 2014-22). Native zoysiagrasses were collected from south-west seaside of Korea from 2010 to 2011. Artificial crossing was conducted to develop F1 hybrid between Z2011 (Z. sinica) and NM1 (Z. matrella) at plastic house in 2011. Among the progenies, 'Seah' showed fine leaf texture and high shoot density from the space planting plots at field. 'Seah' showed genetically light green color, with fine leaf with 1.8mm and height to the lowest leaf blade was 1.94 cm. Ground coverage rate was slower than medium leaf zoysiagrass (Jung-gi), but plant height of 7.1 cm was the lowest among the compared zoysiagrasses and height to lowest leaf of 1.94 cm was lower than most zoysiagrass, which may allow low mowing height.

• Journal of Forest and Environmental Science
• /
• v.15 no.1
• /
• pp.77-88
• /
• 1999

This experiment was carried out to compare the cultural characteristics of mycorrizal fungi associated with Pinus species, and to form mycorrhizal association with Pinus thunbergii by artificial inoculation of these fungi. Mycorrhizal fungi tested showed great variations in cultural characteristics. Most fungal isolates was best grown on MP medium, except PDA for Lepista sp.(Ln73/92). Hagem for Rhizopogon rubescens(FRI91017), and FDA for Paxillus sp.(Pa60/92). Optimum temperature for these fungi was $25^{\circ}C$, except $30^{\circ}C$ for Pisolithus tinctorius(FRI91004 and Pt1). The range of pH conditions favorable for these fungal isolates were also variable from weak acidic(pH5) to weak alkalic(pH8). Utilization of the carbon sources for these mycorrhizal fungi was different. Fructose, glucose, and maltose were all utilized well, while xylose was not utilized generally. Mycelial growth on the media supplemented with potassium nitrate was better than those on other media with urea, asparagine, or peptone as a nitrogen source, and the poor growth was observed on the media with urea. Pisolithus tinctorius(Pt1) among 7 mycorrhizal fungi artificially inoculated for the mycorrhizal synthesis on pinus thunbergii seedlings in the test tube containing a mixture of peat moss-vermiculite(2:1, v/v) formed mycorrhizae successfully after 3 months. P. tinctorius formed branched and unbranched roots covered with thick fungal mantle and radiating extemal hyphae. Mycorrhizal root cross-sectioned by hand, stained, and observed by Nomarski interference microscope showed typical characteristics of ectomycorrhizae: fungal mantle on epidermal cells and thick Hartig net hyphae around cortex cells.

• Journal of Sericultural and Entomological Science
• /
• v.41 no.1
• /
• pp.36-40
• /
• 1999

The optimal condition for the production of the artificial fruiting bodies of Paecilomyces japonica in bulk was determined from the observation of the conidial, hyphal, and cultural condition and the medium. The colony was grown 32 mm diameter in 14 days on potato dextrose agar(PDA). Conidia was irregularly long oval-shaped and measured 4.07${\times}$1.56 ${\mu}$m in average. The hyaline hyphae formed transparent bundles which branched out. The fruiting body on the insect surface was measured 30 to 50 mm in length and formed up to 55 branches. Potato dextrose(PD) both was the most effective for the growth of Paecilomyces japonica among the liquid type media. About 3.1 mg mrcelia in dry weight were produced in 50 ml/PD broth. The best condition for the production of conidia under which condition 4.3${\times}$10$^8$ conidia/ml were harvested was pH 5.0 in acidity and 20$^{\circ}C$ following preculture at 24$^{\circ}C$ in temperature for 7 days.

• Journal of Forest and Environmental Science
• /
• v.18 no.1
• /
• pp.15-30
• /
• 2001

The twenty-first century was entered and began to show the extent where revegetation of the face of slopes using the native woody plant was nationwide at last. Then, future revegetation of the face of slopes was considered on the basis of them in order to attempt the development of this direction, while the recognition for fundamental matter of the revegetation and revegetation of the regional inhabitant was arranged this time. The regional inhabitant sufficiently understood the necessity of the revegetation, and the method using the native woody plant had sufficiently been recognized, and the high evaluation was given in the method. In the other, the major inhabitant questioned it for introducing the exotic species. It was proposed that the utilization of native weeds should be advanced by the pursuit of many facets of the revegetation of the face of the slopes based on these in future in the place where the introduction of the native woody plant is difficult. And, the method for stretching the wire netting for the face of slopes indicated the necessity of the urgent review, because the artificial disaster might be caused. As a conclusion, the goal of the revegetation of the face of slopes confirmed that it was ecologically stable short-terminable face of slopes physically and connects it with the medium and long term for improvement and improvement of global environment, and it indicated the importance of the revegetation of the face of slopes.