• Journal of the Korea Furniture Society
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• v.27 no.3
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• pp.211-219
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• 2016

Culture and design change with time and what they have in common is that both develop in line with the era's living environments. Today, various life styles have emerged to cause changes in housing and living modes and we are in need of designs that can be commonly used in any place. Under the circumstances, furniture plays a role of medium that helps interactions between the human and the space and it has long been used in a variety of ways amid the change of the times. Soban, Korean traditional furniture, is a tool used in our real life and it is a piece of furniture that caters for both arts and functionality. It has been more of a living necessity and accordingly carried wisdom of experience for survival, rather than artificial beauty. Unlike other types of furniture, it is a practical tool designed in consideration of users. Therefore, this study aims to find traditional furniture's wisdom of experience in Soban, which tends to get neglected, and ensure its value as a modern design element, and to contribute to express and study the formative arts of Korean design so that a past living necessity of Soban can overcome its limitation of being floor-seating furniture and become more than an eating tool in it value.

• Proceedings of the Korean Society of Surveying, Geodesy, Photogrammetry, and Cartography Conference
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• 2010.04a
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• pp.277-280
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• 2010

There is a need to convert the old low- or medium-resolution satellite image-based thematic mapping to the high-resolution satellite image-based mapping of GSD 1m grade or lower. There is also a need to generate middle- or large-scale thematic maps of 1:5,000 or lower. In this study, the DEM and orthoimage is generated with the KOMPSAT-2 stereo image of Yuseong-gu, Daejeon Metropolitan City. By utilizing the orthoimage, automatic extraction experiments of land cover information are generated for buildings, roads and urban areas, raw land(agricultural land), mountains and forests, hydrosphere, grassland, and shadow. The experiment results show that it is possible to classify, in detail, for natural features such as the hydrosphere, mountains and forests, grassland, shadow, and raw land. While artificial features such as roads, buildings, and urban areas can be easily classified with automatic extraction, there are difficulties on detailed classifications along the boundaries. Further research should be performed on the automation methods using the conventional thematic maps and all sorts of geo-spatial information and mapping techniques in order to classify thematic information in detail.

• Microbiology and Biotechnology Letters
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• v.22 no.4
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• pp.415-422
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• 1994

The biodegradation of aromatic compounds by a mixed culture GE1 was investigated in an artificial wastewater containing 250 mg/l of benzene, toluene, and phenol in semicontinuous culture. In the control group (no strains) with an aeration rate of 75 ml/l/min, 37% of phenol and 83% of benzene were volatilized during early 24 hrs and toluene was disappeared from the medium within 12 hrs. The biodegradation of benzene and toluene was effective in SB (strains + biofilm) treatment, while phenol was degraded more quickly in SG (strains + glucose) treatment including glucose as an additional carbon source. aromatic compounds added at a concentration of 250 mg/l were completely removed by SG treatment after 16 hrs or 32 hrs, respectively. The removal rate of COD was high as much as 80 mg/l/h in SG treatment during early period, but COD revealed a stable value of 116~140 mg/l after 12 hrs caused by increased biomass. Therefore, it is concluded that the mixed GE1 could be used for the wastewater treatment including aromatic compounds such as benzene, toluene, and phenol.

• Journal of Embryo Transfer
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• v.11 no.1
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• pp.71-80
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• 1996

The envelope of the rnannnalian oocyte plays crucial roles in sperm-oocyte interactions by providing sperm receptors, inducing acrosome reaction and preventing polyspermy. Understanding of properties of the zona pellucida (ZP) is essential for the artificial control of fertility in mammals. This study was carried out to produce and characterize monoclonal antibodies(MAbs) to porcine ZP proteins. Approximately 8,000 ZPs were obtained from follicular oocytes and dissolved in 40$\mu$l of double distilled water. Following immunization through foot-pad injections of Balb /c mice with a ZP solution, the popliteal lymph nodes were recovered at 2 weeks after the last injection. Hybridoma cell lines were established by fusing lymph node cells with P3X63 myeloma cells through selection using HAT medium and screening by immunofluorescence(IF) microscopy on the isolated ZP. Secreted MAbs were found to consist k chains and different heavy chains as evidenced by isotyping. Some of the MAbs demonstrated high specificity to the ZP in IF. The Mabs also showed positive cross reactivity with hamster and mouse eggs, while negative with bovine eggs. The results implicate that the MAbs can be used not only for identification of functional regions of the ZP, but also for elucidation of mechanisms involved in fertilization of mammals. The MAbs will provide basic information on biochemical anatomy of the ZP as well as can be candidates for the future contraceptive vaccines.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.11 no.4
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• pp.565-574
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• 2000

A new ground penetrating radar imaging method for the estimation of buried artificial structures location and their approximate shapes in dispersive lossy ground is investigated. Fundamental idea is based on estimating delayed time and amplitude retrieval coefficients from scattered signals by buried scatterers. Using absolute value integration of each scanning site not only improve the accuracy of measured scattered signal, but also offers convenient ways to extract the image of buried structures. Multi-term Debye model was employed to describe a dispersive and lossy ground medium. We used the finite difference time domain method to discretize the wave equation in continuous form into the machine suitable form. This imaging method uses a new wave path tracing technique in time domain, which is helpful to identify the exact position of buried structures against the ground surface fluctuations.

• Journal of The Korean Society of Agricultural Engineers
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• v.53 no.1
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• pp.63-69
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• 2011

The green algae Scenedesmus acuminatus was cultured in different media: animal wastewater and an artificial culture medium in order to evaluate potential use for tertiary treatment. The experiments were conducted with air flowrate 1~2 L/min at $28{\sim}30^{\circ}C$. The nitrogen and phosphorus showed very similar removal efficiencies (68~77 % and 69~80 % for nitrogen and phosphorus respectively). The optimal fed period was estimated as three days in the semi-continuous experiment. The effects of $CO_2$ (4.5 %) injection on nutrient uptake from animal wastewater (biological treatment effluent) were compared to an air injection under the same conditions of light and photoperiod. The uptake rates of nutrient with air injection were observed 0.009 gN/gChl-a/day, 0.028 gN/gChl-a/day and T-P 0.003 gP/gChl-a/day for nitrate, total nitrogen and phosphorus respectively. The rates were enhanced by addition of $CO_2$ to 0.026 gN/gChl-a/day, 0.076 gN/gChl-a/day and T-P 0.018 gP/gChl-a/day. This study establishes that $CO_2$ addition during nutrient deprivation of microalgal cells may accelerate tertiary wastewater treatment.

• Journal of Sericultural and Entomological Science
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• v.45 no.2
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• pp.116-120
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• 2003

This study was carried out to obtain basic data on physiological characteristics for an artificial cultivation of fruiting body of C. pruinosa. C. pruinosa showed the most favorable growth on the MCM medium. The optimal condition for the mycelial growth was obtained at 25$^{\circ}C$ and pH 5.0, respectively. Carbon sources such as arabinose, mannose, xylose were favorable for stimulating a mycelial growth and fruiting bodies of C. pruinosa. Ammonium nitrate, ammonium citrate and ammonium tartrate of nitrogen sources also appeared to be good in the mycelial growth and fruiting body formation.

• Journal of Microbiology and Biotechnology
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• v.31 no.9
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• pp.1305-1310
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• 2021

Shikimate is a key high-demand metabolite for synthesizing valuable antiviral drugs, such as the anti-influenza drug, oseltamivir (Tamiflu). Microbial-based strategies for shikimate production have been developed to overcome the unstable and expensive supply of shikimate derived from traditional plant extraction processes. In this study, a microbial cell factory using Corynebacterium glutamicum was designed to overproduce shikimate in a fed-batch culture system. First, the shikimate kinase gene (aroK) responsible for converting shikimate to the next step was disrupted to facilitate the accumulation of shikimate. Several genes encoding the shikimate bypass route, such as dehydroshikimate dehydratase (QsuB), pyruvate kinase (Pyk1), and quinate/shikimate dehydrogenase (QsuD), were disrupted sequentially. An artificial operon containing several shikimate pathway genes, including aroE, aroB, aroF, and aroG were overexpressed to maximize the glucose uptake and intermediate flux. The rationally designed shikimate-overproducing C. glutamicum strain grown in an optimized medium produced approximately 37.3 g/l of shikimate in 7-L fed-batch fermentation. Overall, rational cell factory design and culture process optimization for the microbial-based production of shikimate will play a key role in complementing traditional plant-derived shikimate production processes.

• Food Science of Animal Resources
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• v.41 no.2
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• pp.261-273
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• 2021

Leuconostoc mesenteroides H40 (H40) was isolated from kimchi, and its probiotic properties and neuroprotective effect was evaluated in oxidatively stressed SH-SY5Y cells. H40 was stable in artificial gastric conditions and can be attached in HT-29 cells. In addition, H40 did not produce β-glucuronidase and showed resistant to several antibiotics. The conditioned medium (CM) was made using HT-29 cells refined with heat-killed probiotics (Probiotics-CM) and heated yogurts (Y-CM) to investigate the neuroprotective effect. Treatment with H40-CM not only increased cell viability but also significantly improved brain derived neurotropic factor (BDNF) expression and reduced the Bax/Bcl-2 ratio in oxidatively stress-induced SH-SY5Y cells. Besides, probiotic Y-CM significantly increased BDNF mRNA expression and decreased Bax/Bcl-2 ratio. The physicochemical properties of probiotic yogurt with H40 was not significantly different from the control yogurt. The viable cell counts of lactic acid bacteria in control and probiotic yogurt with H40 was 8.66 Log CFU/mL and 8.96 Log CFU/mL, respectively. Therefore, these results indicate that H40 can be used as prophylactic functional dairy food having neuroprotective effects.

• International journal of advanced smart convergence
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• v.11 no.2
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• pp.22-29
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• 2022

In this paper, for the purpose of designing an real-time unmanned monitoring system, the YOLOv5s (small) object detection model was applied on the NVIDIA TX2^TM AI (Artificial Intelligence) edge computing platform in order to design the fundamental function of an unmanned monitoring system that can detect objects in real time. YOLOv5s was applied to the our real-time unmanned monitoring system based on the performance evaluation of object detection algorithms (for example, R-CNN, SSD, RetinaNet, and YOLOv5). In addition, the performance of the four YOLOv5 models (small, medium, large, and xlarge) was compared and evaluated. Furthermore, based on these results, the YOLOv5s model suitable for the design purpose of this paper was ported to the NVIDIA TX2^TM AI edge computing system and it was confirmed that it operates normally. The real-time unmanned monitoring system designed as a result of the research can be applied to various application fields such as an security or monitoring system. Future research is to apply NMS (Non-Maximum Suppression) modification, model reconstruction, and parallel processing programming techniques using CUDA (Compute Unified Device Architecture) for the improvement of object detection speed and performance.