• 한국수정란이식학회지
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• 제25권3호
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• pp.155-159
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• 2010

The objective of this study was to determine physiological and mechanical factors effecting on the pregnancy rates following artificial insemination in dairy cattle. Estrus for artificial insemination of cows was checked whether the outflow of mucus from the elements was out flow or not. There were no significant difference of gestation rates with mucus release (31.76%) and without mucus release (24.03%). The pregnancy rates were 39.02% in 10~20mm of follicle size group and 27.08% (p<0.05) in 20~30mm diameter of the follicles. There were not different pregnancy rates between twice inseminated dairy cattle and more than 3 times inseminated cattle. The pregnancy rate was 28.57% under automatic milking system. In contrast, under artificial milking system pregnancy rate was 56.85%. Two systems significantly (p<0.05) were different. These results suggest that pregnancy rates were not effected by physiological system, but mechanical condition.

• 대한수의사회지
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• 제37권12호
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• pp.1112-1119
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• 2001

This study was carried out to investigate the main causes of sterility influenced on conception rate for artificial insemination in Elk deer. We carried out A.I work for 202 hinds elk deers at breeding season during the period of 02 Sep. thru 01 Oct. of 2

• 농업과학연구
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• 제28권2호
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• pp.85-91
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• 2001

돼지에서 액상정액을 이용한 인공수정이 번식성적에 미치는 영향을 구명하기 위하여 축산기술연구소 종축개량부 (충남, 성환) 돼지 인공수정센터에서 사육중인 인공수정용 종모돈을 이용하여 1995년부터 2000년까지 액상정액을 이용한 인공수정시 종모돈의 품종, 인공수정 횟수, 계절 및 정자농도가 번식성적에 마치는 영향을 조사하여 돼지 인공수정시 번식성적 향상과 실용화에 기여하고자 본 연구를 실시하였다. 액상정액 인공수정시 정액을 생산한 종모돈의 품종 (Landrace, Yorkshire, Duroc)간 번식성적은 유의차가 인정되지 않았다. 발정당 2회 또는 3회 인공수정 시에도 번식성적에 차이가 없었다. 인공수정을 실시한 계절별로 번식성적을 조사하였으나 계절간 차이점은 발견할 수 없었다. 액상정액 인공수정시 1회 주입정자의 최저농도를 구명코자 활력 70% 이상인 정자수를 기준으로 80 ml 병당 정자농도를 3.0, 2.5, 2.0 및 $1.5{\times}10^9$로 조절하여 인공수정한 결과 수태 율, 분만 율, 총산자수 및 생존산자수에서 통계적인 유의차가 인정되지 않아 1회 주입정자농도는 운동성 정자 기준으로 $1.5{\times}10^9/80ml$ 이상으로 조절하면 인공수정에 실용적으로 사용할 수 있음을 보였다.

• 한국수정란이식학회지
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• 제28권2호
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• pp.87-93
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• 2013

The efficiency of artificial insemination (AI) for horses remains unsatisfactory. It is mainly because each process of AI causes a detrimental effect on semen quality. To sustain quality of semen properly, several factors including libido of stallions and sperm damage during sperm processing and preservation should be considered. Stallions with decent libido produce a high ratio of sperm to seminal plasma in their ejaculates, which is the ideal semen composition for maintaining sperm quality. Thus, to maximize the fertility rate upon AI, stallions should be appropriately managed to enhance their libido. Seminal plasma should have a positive effect on horse fertility in the case of natural breeding, whereas the effects of seminal plasma on both sperm viability and quality in the context of AI remain controversial. Centrifugation of semen is performed during semen processing to remove seminal plasma and to isolate fine quality sperm from semen. However, the centrifugation process can also result in sperm loss and damage. To solve this problem, several different centrifugation techniques such as Cushion Fluid along with dual and single Androcoll-E$^{TM}$ were developed to minimize loss of sperm and to damage at the bottom of the pellet. Most recently, a new technique without centrifugation was developed with the purpose of separating sperm from semen. AI techniques have been advanced to deliver sperm to optimal region of female reproductive tract at perfect timing. Recombinant equine luteinizing hormone (reLH) and low dose insemination techniques have been developed to maximize both fertility rate and the efficiency of AI. Horse breeders should consider that the entire AI procedure should be optimized for each stallion due to variation in individual horses for a uniformed AI protocol.

• 한국임상수의학회지
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• 제37권5호
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• pp.235-241
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• 2020

We aimed to determine the effect of timed artificial insemination (TAI) protocols on the pregnancy rate per insemination and pregnancy loss compared with AI performed at detected estrus in dairy cows and Korean Hanwoo cattle under heat stress. In dairy cattle, 1,250 sets of data that underwent AI during heat stress (temperature-humidity index ≥ 72) were categorized according to their TAI protocols or as controls: 1) PGF_2α-36 h-estradiol benzoate (EB)-36 h-TAI (PG-EB group, n = 113); 2) GnRH-7 days-PGF_2α-56 h-GnRH-16 h-TAI (Ovsynch group, n = 455); or 3) GnRH-6 days-Ovsynch (G6G group, n = 136). The remaining cows underwent AI at detected estrus (AIDE group, n = 546). The probability of pregnancy per AI 45 days after AI was higher (P < 0.01) in the PG-EB (odds ratio [OR]: 1.68), Ovsynch (OR: 1.48), and G6G (OR: 1.79) groups than in the AIDE group. However, the prevalence of pregnancy loss between 30 and 45 days after AI did not differ among the groups. In Hanwoo cattle, 617 sets of data inseminated artificially under heat stress were categorized into AIDE (n = 281), PG-EB (n = 194), and combined Ovsynch or G6G (n = 142) groups. The probability of pregnancy per AI 45 days after AI and the prevalence of pregnancy loss between 30 and 45 days after AI did not differ among the groups. Thus, implementation of a TAI protocol (PG-EB, Ovsynch, or G6G) in dairy cows under heat stress improves the pregnancy rate per AI versus AIDE, whereas there is no beneficial effect of TAI on the pregnancy rate of Hanwoo cattle under heat stress.

• 한국가축번식학회지
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• 제3권2호
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• pp.57-63
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• 1979

In this study, pooled whole semen and diluted semen with skim milk lactose solution and yolk skim milk lactose solution were inseminated at 6 and 7 days interval during 90 days. Egg production, fertility and hatchability were investigated. The results obtained from this study are summarized as following: 1. The average fertility of 57.8% for whole semen was clearly higher than that of 35.2% for diluted semen during 90 days insemination trial. 2. The highest fertility was a, pp.ared from 35 to 99 days after insemination for the whole semen, but from 28 to 60 days for the diluted semen during 90 days insemination trial. 3. In case of 7 days insemination interval, highest fertility of 86.6 and 70.0% for the whole semen and the diluted semen was a, pp.ared on 2 days after insemination and thereafter the fertility was gradually decreased according to passage of insemination. The lowest fertility of 35.0 and 0.0% for the whole semen and diluted semen was a, pp.ared on 1 day after insemination. 4. In case of 6 days insemination interval, highest fertility of 80.0 and 55.8% for the whole semen and the diluted semen was also a, pp.ared on 2 days after insemination and thereafter the fertility was slowly decreased according to passage of insemination. However, lowest fertility of 25.0 and 20.0% for the whole semen and the diluted semen was a, pp.ared on 0 day after insemination. 5 It suggests that there was no difference in fertility between the skim milk lactose and the yolk skim milk lactose dilutors. 6. In case of whole semen, average fertility of 7 days insemnaition interval was a, pp.rently lower than that of 6 days, however there was no difference in fertility between 6 and 7 days insemination interval. 7. Insemination interval of 6 and 7 days and passage day after insemination did not alter egg production and hatchability of fertilized egg production and hatchability of fertilized egg in both whole and diluted semen.

• 가축개량기술정보
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• 9호
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• pp.1-12
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• 1995

• 가축개량기술정보
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• 20호
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• pp.1-12
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• 2001

• 가축개량기술정보
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• 11호
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• pp.1-12
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• 1996

• 가축개량기술정보
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• 15호
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• pp.1-12
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• 1997