• Journal of Korean society of Dental Hygiene
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• v.5 no.1
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• pp.123-130
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• 2005

This study was executed to evaluate the effects of natural herbal medicine extract on the antimicrobial activity and the artificial plaque formation. Schisandra chinensis, Sophora flavescens, Dryopteris crassirhizoma and Aristolochia contorta were extracted by 80% MeOH respectively and each concentration(5mg, 10mg, 20mg) of herbal medicine extract was tested to show the antimicrobial activity against S. mutans. To show the inhibition effect of herbal medicine extract on the artificial dental plaque formation each extract of 0.4% concentration was added into M 17 broth and inoculated with $5.0{\times}10^6$ of S. mutans. After 24 hour incubation each weight of artificial dental plaque was measured and compared with each other. Results obtained are as follows: 1. Dryopteris crassirhizoma showed very intense antimicrobial effect but Schisandra chinensis, Sophora flavescens and Aristolochia contorta showed a little difference against control. 2. Regarding artificial plaque formation inhibiting activity by each herbal extract at 0.4% concentration, Dryopteris crassirhizoma showed the perfect inhibition effect. Schisandra chinensis and Aristolochia contorta followed in order but Sophora flavescens showed the worst value. In conclusion, Dryopteris crassirhizoma showed strong antimicrobial effect against S. mutans and significant inhibiting effect on the artificial dental plaque formation.

• Journal of Korean society of Dental Hygiene
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• v.21 no.1
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• pp.17-25
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• 2021

Objectives: Interdental toothbrushes are made and sold in various design types and brush thicknesses. However, there is little research on which type of interdental toothbrush currently manufactured and sold on the market is the most effective in eliminating interdental plaque. Therefore, this study aims at comparing the elimination rate of artificial dental plaque between reciprocating- and rotating-types of interdental toothbrush based on the frequency of application and thickness of brush. Methods: This study focused on the effective management of dental plaque using interdental toothbrush, a recommended item for personal dental hygiene. The method was as follows: artificial dental plaque coloring was applied to the distal surface of artificial tooth #46 and the mesial surface of #47. The area was subject to reciprocal movement three and six times to eliminate artificial plaque. Results: The results showed that using a 0.7mm rotating interdental toothbrush, on the proximal surface of each molar, the elimination rates were: on the distal surface of #46, upon three applications 40.24%, upon six applications 30.41%; on the mesial surface of #47, upon three applications 44.52%, upon six applications 29.72%. Conclusions: These results showed that for rotating-type interdental toothbrushes, a high dental plaque elimination rate was observed even though many reciprocal movements were not performed.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.34 no.3
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• pp.325-340
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• 2008

The purpose of this study was to evaluate the artificial dental plaque by Streptococcus mutans on 4 different implant surfaces. In this study, the specimens were divided into 4 groups according to implant surface treatment. Uncoated implant group(n=5) which has an uncoated, smooth surfaced implant(Osstem, Korea), SLA implant group(n=5) which has an sandblasted large grit and acid-etched surface implant(Bicon, USA). Oxidized implant group(n=5) which has an oxidized surfaced implant (Osstem, Korea), and RBM implant group(n=5) which has resorbable blasting media(RBM) surfaced implant(Osstem, Korea). Acquired pellicle by human saliva and dental plaque by Streptococcus mutans were made on each implant surface. To analyze the plaque condition on implants surfaces, cell count and optical density were taken as a microbiologic method, and SEM(Scanning Electronic Microscope) findings was also taken for evaluation of surface condition. The following results were obtained. 1. Cell counting results of artificial dental plaque were Uncoated group($658.0{\pm}102.0$), RBM group($878.0{\pm}170.0$), SLA group ($946.0{\pm}42.0$), Oxidized group($992.0{\pm}40.0$), and there was difference between Oxidized group and Uncoated implant group(p<0.05). In case of modified cell counting results by v/w% were RBM group($197.8{\pm}45.2$), Oxidized group($207.04{\pm}8.34$), Uncoated group($261.6{\pm}40.6$), SLA group($315.4{\pm}14.0$), and there was difference between RBM group and SLA group(p<0.05). 2. Optical density results of artificial dental plaque after ultrasonic treatment was that there was difference among groups, and optical density of RBM group was higher than that of Uncoated group(p<0.05). In case of modified optical density results by v/w%, there was difference among groups, and the modified optical density of Uncoated group and SLA group was higher than those of Oxidized group and RBM group(P>0.05). 3. SEM findings of artificial dental plaque on the surfaces of implant as follows; there were artificial dental plaque on the surfaces of all test implants. Streptococcus mutans and by-product were observed at 10,000 times magnified condition on all test implants. Adhesion area of artificial dental plaque was about 1/2 of total surface after 24 hours incubate at $37^{\circ}C$. These results showed that there were differences among implant surfaces on the growth of Streptococcus mutans, and bacteria and by-product were covered about 1/2 area of total implant surfaces at 24 hours incubate at $37^{\circ}C$.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.29 no.4
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• pp.309-320
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• 2007

Purpose: This study was to evaluate the removal effect on artificial plaque from RBM treated implant surfaces that are exposed due to peri-implantitis. Materials and methods: Artificial plaque with Streptococcus mutans and acquired pellicle adhered to RBM treated implant discs. Study materials divided into one control and six test groups. In test groups, physical and chemical methods used to remove plaques. Prophyflex, Professional Mechanical Tooth Cleaning (PMTC) and interdental brush as mechanical treatments and 0.1% Chlorhexidine, Citric acid, HCl tetracycline as a chemical treatment were used. To analyses the study, disc weight was measured for remaining plaque quantities and SEM(Scanning Electronic Microscope) findings was taken for evaluation of surfaces. Results: 1. In weight changes, there was significant difference between each treatment group and the control group (p<0.05). Therefore all treatment methods using this study have good ability for remove plaques. 2. In weight changes, there was no significant difference between mechanical and chemical group, and there were no significant differences between each groups (p>0.05). 3. SEM findings after mechanical treatment disclosed as follows; Prophyflex group looked like sound implant surface, and there were some paste on implant surface at PMTC group, and there were some artificial plaque at interdental brush group. 4. SEM findings after chemical treatment disclosed as follows; there were some dark lesions which were supposed as the product from Streptococcus mutans at Chlorhexidine, Citric acid and HCl tetracycline groups. Conclusion: All six methods using in this study have good ability to remove artificial plaque on RBM treated implant. According to SEM findings, prophyflex is a superior method for removing of dental plaque among test groups.

• The Journal of Korean Academy of Prosthodontics
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• v.59 no.3
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• pp.291-298
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• 2021

Purpose. The purpose of this study is to compare five interdental cleansing products' effectiveness on removing artificial dental plaque on the interdental space of zirconia crowns. Materials and methods. A model with abutments on the right mandibular second premolar and first molar were prepared. 10 zirconia crowns for each abutment were fabricated. After applying artificial dental plaque between the zirconia crowns, a single clinician attempted to remove the plaque with five products: interdental toothbrush, end-tuft toothbrush, dental floss, Easypick, Water pik. They were conducted 10 times per group. The aspect and area of removed surfaces were analyzed using images taken with a digital camera. One factor analysis of variance was performed as a statistical analysis, and a post-hoc test was performed using the Scheffé method (P < .05). Results. There were differences in the area and the pattern according to the characteristics of the products. The largest area, including the marginal portion, was removed in the dental floss group. Interdental toothbrush group was the most effective in removing the dental plaque at the marginal portion. Easypick was less effective than the interdental toothbrush. The end-tuft toothbrush showed better results than other products in cleansing mesiobuccal and distobuccal area, but could not cleanse the area directly below the contact point. In Water pik group, artificial dental plaque was scarcely removed. The removal rate of artificial dental plaque was in the order of floss (69.47%), end-tuft toothbrush (49.36%), interdental toothbrush (44.20%), Easy pick (13.04%), and Water pik (0.59%). Conclusion. Dental floss showed the highest removal rate in the interdental space restored with zirconia crowns, while interdental toothbrush was the most effective in removing the dental plaque at the marginal portion.

• Journal of the korean academy of Pediatric Dentistry
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• v.30 no.1
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• pp.25-32
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• 2003

S. mutans is the most important causative bacteria of dental caries among the oral bacteria. S. salivarius is a normal inhabitant in the human oral cavity. Nine strains of S. salivarius in this study were isolated from the oral cavities of children and identified, and their effect on S. mutans and S. oralis was studied. 1. The mean weight of produced artificial plaque on the wires in the beaker was 204.9 mg in the culture of S. mutans only, whereas being reduced to 1.9 mg through 20.6mg in the combined culture of S. mutans and each S. salivarius isolate (p<0.05). The viable cell didn't show the difference between them after culturing. 2. When S. mutans was cultured in the media containing culture supernatant of each S. salivarius isolate in M17 broth, the mean weight of produced artificial plaque was 117.1 mg on the wires, whereas being 47.7 mg in the media containing culture supernatant of each S. salivarius isolate in M17 broth containing 5% sucrose. 3. The polymer produced by S. salivarius isolates was on the thin layer chromatography. 4. Inulin and levan didn't inhibit the formation of artificial plaque by S. mutans in the beaker test. These results suggested that fructan-producing S. salivarius isolates inhibited the formation of artificial plaque by S. mutans.

• Journal of the korean academy of Pediatric Dentistry
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• v.25 no.3
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• pp.619-626
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• 1998

The critical etiologic factor in the development of dental caries is dental plaque. The main component of dental plaque is the mutan produced by Streptococcus mutans. The following results were obtained by using blue mutan to assess the factors affecting the mutan-digesting activity of Micromonospora aurantiaca isolated from oral cavity. Micromonospora aurantiaca digested more blue mutan in the minimal essential broth at pH 7.0 than at pH 5.5 or 8.5, and at $37^{\circ}C$ than at $32^{\circ}C\;or\;42^{\circ}C$. Blue mutan was similarly digested at the range of 1mM to 16mM of $CaCl_2$ and 0.1mM to 6.4 mM of $MgCl_2$, while being significantly digested at the concentration of 2.5mM of KCl. When the concentration of glucose was decreased in the minimal essential broth, the digestion of blue mutan was increased. When the culture supernatant of Micromonospora aurantiaca in the RL broth with 1% glucose or 0.5% mutan was mixed with 2 ${\times}$ BHIYS broth containing 0.5% yeast extract and 10% sucrose, the formation of artificial plaque on the orthodontic wires by Streptococcus mutans was inhibited(p<0.05). These results indicated that the production of mutanase was identified in the culture supernatant of Micromonospora aurantiaca, suppressing the formation of artificial plaque by Streptococcus mutans.

• Journal of Korean society of Dental Hygiene
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• v.11 no.4
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• pp.465-474
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• 2011

Objectives : The removal of most reliable mechanical dental plaque that is to say tooth brushing was generalized to control of dental plaque, many oral health goods have also developed due to the effect differences followed by individual habit. The electric toothbrush have studied and developed widely as major field of study that electric toothbrush having various moving phase was sold and developed at the market. Methods : Accordingly author studied about selling electric toothbrushes shape (vibration type, ultra-sonic minuteness vibration type, semi rotation type) to raise the efficiency after comparing to the moving them that total 8 groups classified by poor tooth models for example normal set of tooth, crowding tooth, bracket attached tooth, prosthetic status etc. and executed plaque removal effect on the tooth through comparing experiment. Results : The removal rate of artificial plaque on the tooth was improved in proportion to the increase of tooth brushing time(p<0.05). The ultra-sonic minuteness vibration and semi rotation type was superior to toothbrush of vibration type comparing to the removal rate of plaque on the tooth(p<0.05). Conclusions : The electric toothbrush of supersonic minuteness vibration and semi rotation type can be recommended most of tooth types regardless of oral tooth setting status for example, normal set of tooth, crowding tooth, bracket attached tooth, porcelain tooth.

• The Journal of the Korean Society for Microbiology
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• v.35 no.1
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• pp.77-85
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• 2000

Streptococcus mutans is the most important causative bacteria of dental caries among the oral bacteria. Lactococcus lactis 1370 was isolated from the oral cavity of child. The effect of Lactococcus lactis 1370 on the formation of artificial plaque by Streptococcus mutans was studied. 1. The insoluble substances and bacteria were much more attached on the wall of disposable cuvette in the culture of Streptococcus mutans than in the combined culture of Streptococcus mutans and Lactococcus lactis 1370. 2. The mean weight of produced artificial plaque on the wires in the beaker was 131.7 mg in the culture of Streptococcus mutans only, whereas being reduced to 6.4 mg in the combined culture of Streptococcus mutans and Lactococcus lactis 1370 (p<0.05). The viable cell didn't show the significant difference between them after culturing. 3. When Streptococcus mutans was cultured in the media containing culture supernatant of Lactococcus lactis 1370 cultured in M17 broth containing 0.5% yeast extract and 5% sucrose, the mean weight of produced artificial plaque was 8.0 mg on the wires, whereas being 125.4 mg in the media without culture supernatant of Lactococcus lactis 1370 (p<0.05). The viable cell didn't show the significant difference between them after culturing. 4. When Streptococcus mutans was cultured in the media containing soluble polymer produced by Lactococcus lactis 1370, the mean weight of produced artificial plaque was significantly reduced compared with being cultured in the media without soluble polymer (p<0.05). The viable cell didn't show the significant difference between them after culturing. 5. The soluble polymer produced by Lactococcus lactis 1370 was glucan. 6. The glucan produced by Lactococcus lactis 1370 was water-soluble glucan containing ${\alpha}$-1,6-glucose linkage as the main linkage. These results suggest that the artificial plaque formed by Streptococcus mutans is inhibited by water-soluble glucan produced by Lactococcus lactis 1370.

• Imaging Science in Dentistry
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• v.54 no.1
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• pp.43-48
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• 2024

Purpose: Oral hygiene, maintained through plaque control, helps prevent periodontal disease and dental caries. This study was conducted to examine the accuracy of plaque detection with an intraoral scanner(IOS) compared to images captured with an optical camera. Materials and Methods: To examine the effect of color tone, artificial tooth resin samples were stained red, blue, and green, after which images were acquired with a digital single-lens reflex (DSLR) camera and an IOS device. Stained surface ratios were then determined and compared. Additionally, the deviation rate of the IOS relative to the DSLR camera was computed for each color. In the clinical study, following plaque staining with red disclosing solution, the staining was captured by the DSLR and IOS devices, and the stained area on each image was measured. Results: The stained surface ratios did not differ significantly between DSLR and IOS images for any color group. Additionally, the deviation rate did not vary significantly across colors. In the clinical test, the stained plaque appeared slightly lighter in color, and the delineation of the stained areas less distinct, on the IOS compared to the DSLR images. However, the stained surface ratio was significantly higher in the IOS than in the DSLR group. Conclusion: When employing IOS with dental plaque staining, the impact of color was minimal, suggesting that the traditional red stain remains suitable for plaque detection. IOS images appeared relatively blurred and enlarged relative to the true state of the teeth, due to inferior sharpness compared to camera images.