• 제목/요약/키워드: artificial culture media

검색결과 151건 처리시간 0.027초

노란달걀버섯 균주의 여러 환경 조건에 따른 균사 생장 특성 (Mycelial growth properties of an Amanita javanica strain under various culture conditions)

  • 강정아;가강현;김준영;윤성탁;김성환
    • 한국버섯학회지
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    • 제17권4호
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    • pp.191-196
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    • 2019
  • 노란달걀버섯은(Amanita javanica) 국내에서 산림법으로 보호받고 있는 식용 가능한 외생균근성 버섯이나, 중요한 산림자원으로서 활용하기 위한 기초적인 특성 연구에 대한 자료는 부족한 실정이다. 이에 본 연구는 국내 채집 야생버섯 자실체로부터 분리한 노란달걀버섯 NIFoS 1267 균주를 이용하여 PDA 배지 상에서 물리적 요인(온도, pH, 광)과 화학적인 요인(염분, 중금속, 농약)에 따른 균사생장 특성을 조사하였다. 최적의 물리적 환경은 온도가 30℃, pH가 5-6, 암조건으로 배양이었을 때 노란달걀버섯 균주의 균사생장이 가장 좋은 것으로 나타났다. 화학적 요인으로서 염분은 2.0% 농도 조건까지 버섯균주의 균사 생장이 가능하였다. 50 ppm 농도의 중금속 이온 환경에서 비소(As) 이온은 노란달걀버섯 균주의 균사생장에 영향을 주지 않았으나 카드뮴(Cd)와 납(Pb) 이온은 균사 생장이 불가능하게 하였다. 국내 산림에 사용되고 있는 2가지 농약의 경우, Abamectin 첨가 배지에서는 노란달걀버섯 균주의 균사 생장에 영향이 없었으나 Acetamiprid, Emamectin benzoate, Thiacloprid가 첨가된 배지 환경에서는 균사 생장이 모두 다 저해 되었다. 본 연구 결과는 향후 상업적 생산을 위한 새로운 자원으로서 노란달걀버섯의 인공재배 연구에 도움이 될 것으로 기대된다.

풀버섯의 균사배양 적합조건 및 우량균주 선발 (The Suitable Condition for Mycelial Growth of Volvariella volvacea strains and Selection of the Superior strain)

  • 장명준;이한범;김정한;이윤혜;주영철
    • 한국균학회지
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    • 제37권2호
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    • pp.173-180
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    • 2009
  • This study was carried out to obtain basic data on mycelial growth characteristics for an artificial cultivation of Volvariella volvacea. Twelve URP primers were used to assess genetic relationship of V. volvacea and its result was divided into two groups. But there was nothing different in morphological characteristics in V. volvacea. Among five kinds of mushroom media, MCM medium was selected as the favorable culture medium. The optimal range of temperature and pH for mycelial growth on V. volvacea were 35oC and pH 6~8, respectively. Carbon sources had not an effect on 10 strains of V. volvacea, and nitrogen source for the optimum mycelial growth was yeast extract. Also, we selected GMVV 79004 as a superior strain of 10 strains V. volvacea based on the mycelial growth and yield.

生長點 培養에 依한 민초피나무(Zanthoxylum piperitum var. inerme Makino)의 器內 大量 增殖 및 土壤 活着 (In Vitro Mass Propagation and Soil Adjastment of Zanthoxylum piperitum var. inerme Makino through Apical Meristem Culture)

  • 정우규;이상래
    • 한국자원식물학회지
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    • 제6권2호
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    • pp.171-179
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    • 1993
  • This study was conducted to investigate the effect of growth regulators and medium composition on the growth of each stage in apical meristem culture for mass propagation of Zanthoxylum piperitum var. inerme Makino. The source material, shoot tip segments were taken from three-years old graft trees. Apical meristems were cultured in vitro on basal MS, GD, WS, half strength MS(1/2MS) and half strength GD(1/2GD) media supplemented with various concentrations for growth regulators(BA, IBA) and inorganic nutrients. The results summarized are as follows: 1. In culture establishment stage, ratio of culture establishment was 96.7% and the best resuit was obtained using MS medium supplemented with 1.0mg/l BA and 0.2mg/l IBA. 2. In shoot multitication stage, both shoot multiplication and growth were achieved in average 5.6cm. These results were obtained on in MS medium supplemented with 1.0mg/l BA and 0.2mg/l IBA. 3. In roothing stage, phloroglucinol(PG) acted as IBA synergist in root initiation. The most faverable combinations for root development was half-strength MS medium supplemented with 162mg/l PG and 0.2mg/l IBA, and ratio of rooting was 58.0%. 4. In Vitro formed plantlets were transplanted to paper pots in greenhouse with 85% of relative humidity. 96% of survival rate was obtained from artificial soil mix having same volume of sand, vermiculite, peat, and soil.

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Innovation and craft in a climate of technological change and diffusion

  • Hann, Michael A.
    • 복식문화연구
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    • 제25권5호
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    • pp.708-717
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    • 2017
  • Industrial innovation in Britain, during the eighteenth and nineteenth centuries, stimulated the introduction of the factory system and the migration of people from rural agricultural communities to urban industrial societies. The factory system brought elevated levels of economic growth to the purveyors of capitalism, but forced people to migrate into cities where working conditions in factories were, in general, harsh and brutal, and living conditions were cramped, overcrowded and unsanitary. Industrial developments, known collectively as the 'Industrial Revolution', were driven initially by the harnessing of water and steam power, and the widespread construction of rail, shipping and road networks. Parallel with these changes, came the development of purchasing 'middle class', consumers. Various technological ripples (or waves of innovative activity) continued (worldwide) up to the early-twenty-first century. Of recent note are innovations in digital technology, with associated developments, for example, in artificial intelligence, robotics, 3-D printing, materials technology, computing, energy storage, nano-technology, data storage, biotechnology, 'smart textiles' and the introduction of what has become known as 'e-commerce'. This paper identifies the more important early technological innovations, their influence on textile manufacture, distribution and consumption, and the changed role of the designer and craftsperson over the course of these technological ripples. The implications of non-ethical production, globalisation and so-called 'fast fashion' and non-sustainability of manufacture are examined, and the potential benefits and opportunities offered by new and developing forms of social media are considered. The message is that hand-crafted products are ethical, sustainable and durable.

Effect of Various Sawdusts and Logs Media on the Fruiting Body Formation of Phellinus gilvus

  • Jo, Woo-Sik;Rew, Young-Hyun;Choi, Sung-Guk;Hwang, Mi-Hyun;Park, Seung-Chun;Seo, Geon-Sik;Sung, Jae-Mo;Uhm, Jae-Youl
    • Mycobiology
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    • 제35권1호
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    • pp.6-10
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    • 2007
  • Present experiments were conducted to determine the possibility of artificial culture with various sawdust of P. gilvus. The pH value was 6.0 of oak sawdust, 6.5 of mulberry sawdust, 6.6 of elm sawdust, 6.3 of acacia sawdust and 6.1 of apple tree sawdust. Mycelial density on elm sawdust and acacia sawdust were lower than those of oak sawdust, and apple sawdust. Weight of fresh fruiting body showed that 179 g on oak tree, 227 g on oak sawdust, 21 g on elm tree, 76 g on elm sawdust, 106 g on apple tree, and 170 g on apple sawdust. Among them, the yield of oak substrates was the highest whereas acacia sawdust was the lowest, and it is concluded that the yields of sawdust substrates were higher than log substrates. P. gilvus grown on various sawdusts and logs used in this study have shown similar in anti-tumor activity against P388.

축산폐수 고도처리를 위한 미세조류 Scenedesmus acuminatus의 이용 가능성 (Potential Use of Microalgae Scenedesmus acuminatus for Tertiary Treatment of Animal Wastewater)

  • 박기영;임병란;이기세;이수구
    • 한국농공학회논문집
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    • 제53권1호
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    • pp.63-69
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    • 2011
  • The green algae Scenedesmus acuminatus was cultured in different media: animal wastewater and an artificial culture medium in order to evaluate potential use for tertiary treatment. The experiments were conducted with air flowrate 1~2 L/min at $28{\sim}30^{\circ}C$. The nitrogen and phosphorus showed very similar removal efficiencies (68~77 % and 69~80 % for nitrogen and phosphorus respectively). The optimal fed period was estimated as three days in the semi-continuous experiment. The effects of $CO_2$ (4.5 %) injection on nutrient uptake from animal wastewater (biological treatment effluent) were compared to an air injection under the same conditions of light and photoperiod. The uptake rates of nutrient with air injection were observed 0.009 gN/gChl-a/day, 0.028 gN/gChl-a/day and T-P 0.003 gP/gChl-a/day for nitrate, total nitrogen and phosphorus respectively. The rates were enhanced by addition of $CO_2$ to 0.026 gN/gChl-a/day, 0.076 gN/gChl-a/day and T-P 0.018 gP/gChl-a/day. This study establishes that $CO_2$ addition during nutrient deprivation of microalgal cells may accelerate tertiary wastewater treatment.

현악사중주 공연의 역사와 미래: 미디어와 인공지능을 활용한 융합 공연의 가능성에 대하여 (The History and Future of String Quartet Performances: Examining the Possibility of Convergent Performances Employing Media and Artificial Intelligence)

  • 박은지
    • 문화기술의 융합
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    • 제9권5호
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    • pp.697-706
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    • 2023
  • 본 연구는 현악사중주의 역사를 살펴보고, 현대에 제시된 융합 공연을 분석하여 미래의 청중이 수용할만한 새로운 공연의 패러다임을 제안하는 것을 목표로 한다. 연구의 과정에서는 과거와 현대의 현악사중주가 어떻게 발전했는지를 면밀하게 살펴보고, 그 과정에서 나타난 청중의 변화에 관하여 분석한다. 더불어 현대 현악사중주의 기술 융합 공연 사례로부터 새로운 청중의 수요에 따른 오늘날의 클래식 공연산업이 어떠한 변화를 맞을 수 있을지를 모색한다. 연구의 결과로 현대의 현악사중주는 미디어와 AI 기술의 융합을 통한 새롭고 독창적인 방향의 공연이 필요하다는 결론을 내렸다.

눈꽃동충하초 배지별 인공재배법과 성분분석에 관한연구 (Study on analysis of components and artificial cultural practice on several culture media of Paecilomyces japonica)

  • 이희덕;김용균;김홍규;이가순
    • 한국자원식물학회지
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    • 제12권2호
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    • pp.102-106
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    • 1999
  • 1. 누에를 이용한 눈꽃동충하초 재배에서 종균접종은 누에 5령1일째 실시하였으며, 감염율은 72% 되었으며 2. 눈꽃동충하초 대량생산을 위한 인공재배 최적배지는 "현미80g+번데기가루20g" 처리구에서 자실체 길이 11.4cm,무게 51.6g, 짙노랑색으로 아주 양호했으며, 현미 처리구, 번데기+PDA처리구에서도 양호했음. 3.누에동충하초에서 발생된 눈꽃동충하초는 번데기 1마리당 1.2g(번데기포함), 길이 3.0~3.5cm정도 되었으며, 일부는 누에상태에서도 동충하초가 발생하였음. 4. $\beta$-glucan함량은 누에 5령 1일 접종 처리구가 40.5%로 가장 높고 저비용 배지인 현미 16.4%, 번데기 20.7%, 현미+번데기가루 23.1%, 소나무 톱밥+밀기울 28.7%로 $\beta$-glucan 함량이 높았다. 5. 지네, 굼벵이, 누에가루처리구에서는 균사생육이 부진하였고 분생포자만 발생하였다.

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Stress as a Trigger of Pollen Embryogenesis

  • Zarsky, Viktor;Soukupova, Hana
    • 식물조직배양학회지
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    • 제27권5호
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    • pp.411-413
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    • 2000
  • The ability of microspores or young pollen grains (male gametophytes) to undergo developmetal switch to embryogenic (sporophytic) pathway exemplifies the concept of totipotency as applied to haploid posmeiotic cells. As a first step pollen is devoid of positional information provided in situ by the intact anther - by isolation and cultivation in vitro in artificial media. This is inevitably accompanied by some degree of stress response in microspore/pollen. It has been shown in both monocots and dicots that intentional stress treatment (mostly starvation or heat shock) greatly stimulates embryo induction rate. Using transgenic sHSP antisense Nicotiana tabacum we show that expression of small heat shock proteins is an integral part of successful embryo and later haploid plant production from pollen grains. Our recently published data show that sHSP chaperone function is optimal in the absence of ATP.

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감나무 둥근무늬낙엽병균(Mycosphaerella nawae)의 분생포자 대량 형성법 (Mass Sporulating Method for Conidial Formation of Mycosphaerella nawae Causing the Spotted Leaf Casting of Persimmon)

  • 권진혁;강수웅;김동길;박창석;김희규
    • 한국식물병리학회지
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    • 제13권4호
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    • pp.255-256
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    • 1997
  • 감나무 둥근무늬낙엽병균(Mycosphaerella nawae)의 분생포자를 대량 형성시키는 방법을 조사한 결과 분생포자는 감자한천배지(PDA)를 $25^{\circ}C$ 항온기에 90일간 배양시 $39.0{\times}10^4/ml$의 분생포자가 형성되었다.

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