• Korean Journal of Plant Tissue Culture
• /
• v.28 no.5
• /
• pp.273-276
• /
• 2001

In order to develop effective acclimatization methods for Aralia elata plantlets regenerated from somatic embryos, various acclimatizing conditions were compared regarding both survival rate and growth of the plantlets. The plantlets were transplanted into plastic boxes containing artificial soil in the presence of either several levels of MS liquid media, distilled water, 2% sucrose or 0.1% hyponex solution. They were then cultured by spraying of distilled water twice a week and maintained in the normal tissue culture room. Perlite was proved to be better than vermiculite on survival rate and growth of the plantlets. As the size of perlite (larger than 0.2 cm in diameter) increased, both the survival rate and growth of the plantlets improved. Among the various MS liquid media and different aqueous solutions tested, distilled water appeared to result in the best survival rate and growth. MS media were also effective in increasing survival rate and supporting growth when diluted to 1/4 and/or 1/8. The acclimatized plantlets could be transplanted directly onto the nursery bed and grown normally. The above results suggest that plantlets regenerated from somatic embryos of Aralia elata be effectively acclimatized using a plastic box containing perlite with distilled water treatment.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 1998.10a
• /
• pp.126-128
• /
• 1998

• Journal of Bio-Environment Control
• /
• v.2 no.1
• /
• pp.37-45
• /
• 1993

The purpose of this research was to develop ideal substrates for the production of good quality tomatoes in bag culture system and also to improve media with low or no environmental pollution by blending and mixing artificial substrate including peatmoss, perlite, vermiculite, granular rockwool, polyphenol resin foam, bark, and smoked rice hull. The highly efficient media proved by experiments were vermiculite, smoked rice hull, polyphenol resin foam, granular rockwool, and perlite, which showed good results in the early growth as well as the marketable yield of tomato in the bag culture. Tomato plants grown in the media mixed with peatmoss, vermiculite and granular rockwool at the ratio of 3 : 1 : 1(by volume) showed the highest marketable yield, and the next at the ratio of 2 : 1 : 1. The perlite-granular rockwool mixtures at the ratio of 2 : 3 and 1 : 4, and the peatmoss vermiculite mixtures at the ratio of 2 : 3 and 3 : 2, seemed to be promising media for bag culture.

• Mycobiology
• /
• v.35 no.4
• /
• pp.230-234
• /
• 2007

In order to breed a Cordyceps bassiana isolate that stably forms fruiting body in artificial cultivation, isolates derived from subculturing and single spores were tested through mating. From C. bassiana EFCC 783, three subcultured isolates EFCC 2830, EFCC 2831 and EFCC 2832 were obtained and fourteen single conidial isolates were obtained from these three subcultured isolates. Two different morphological types were found in the fourteen single conidial isolates. One type was able to form synnemata and another type was not able to form synnemata. Since switch of morphological type was not observed despite their continuous subculturing, cross was performed between the two types and the formation of fruiting body was examined. Ascospores were obtained from a selected fruiting body formed by hybrid of the cross. Self-cross and combinational cross of the ascospore-derived isolates generated hybrids that stably produce high quality fruiting body in artificial media.

• Journal of Bio-Environment Control
• /
• v.9 no.1
• /
• pp.47-59
• /
• 2000

In order to investigate the physical and chemical properties of artificial culture media, the specimens were substituted with 5~20％ clay, 10~30％(w) quick lime, 5~l5％(w) burnt plaster and 10％(w) sawdust. Fly ash-clay bodies were sintered at 1,050~1,20$0^{\circ}C$ and then their properties were determined. It was found that 90FA10JC(fly ash ＋clay(90：10, ％(w)) specimen sintered at 1,15$0^{\circ}C$ for 10 min. had good physical and chemical properties. When this composition was supplement with 10％(w) sawdust, bulk density water absorption, apparent porosity, compressive strength and pH after 240 hrs curing time were 1.14, 54.4％, 39.5％, 54 kgf.cm$^{-2}$ and 7.1 respectively. The physical properties of fly ash-quick lime-burnt plaster system specimens were superior to FAJC systems. However, this composition we not suitable as a artificial culture media because of its high pH. In this study, it was shown that 90FA10JC10SD(90FA10JC ＋10％(w) sawdust) system exhibited the best physical properties.

• Journal of Plant Biotechnology
• /
• v.30 no.3
• /
• pp.269-273
• /
• 2003

In order to develop an efficient micropropagation protocal for Eucalyptus pellita, on in vitro culture system has been was established by inducing axillary buds from greenhouse stock materials. Among 6different media tested, DKW medium was the best ot induce bast induce both shoot proliferation and growth. Average number of proliferated shoots of 403per explant was obtained at the concentration of 0.1mg/LBA. Most of the stem materials excreted phenolic compounds at the proximal part of the explant and caused darking of the media. Therefore, it was necessary to transfer frequently to a fresh medium and/or to add activated charcoal at the concentration of 0.02％(w/v). Generally on vitro roots were formed easily on 1/2DKW medium with NAA treatment. All the explants rooted at the medium containing 0.2mg/L NAA and displayed vigorous root growth in vitro culture conditions. After transferred to an artificial soil mixture (peatmoss: vermiculrite: perlite, 1:1:1, v/v/v) in the greenhouse, most rooted plantlets survived well without any morphological abnormalities. The results show that the species can be micropropagated effectively by the application of axillary bud culture system.

• Clinical and Experimental Reproductive Medicine
• /
• v.49 no.4
• /
• pp.225-238
• /
• 2022

The ultimate goal of human assisted reproductive technology is to achieve a healthy pregnancy and birth, ideally from the selection and transfer of a single competent embryo. Recently, techniques for efficiently evaluating the state and quality of preimplantation embryos using time-lapse imaging systems have been applied. Artificial intelligence programs based on deep learning technology and big data analysis of time-lapse monitoring system during in vitro culture of preimplantation embryos have also been rapidly developed. In addition, several molecular markers of the secretome have been successfully analyzed in spent embryo culture media, which could easily be obtained during in vitro embryo culture. It is also possible to analyze small amounts of cell-free nucleic acids, mitochondrial nucleic acids, miRNA, and long non-coding RNA derived from embryos using real-time polymerase chain reaction (PCR) or digital PCR, as well as next-generation sequencing. Various efforts are being made to use non-invasive evaluation of embryo quality (NiEEQ) to select the embryo with the best developmental competence. However, each NiEEQ method has some limitations that should be evaluated case by case. Therefore, an integrated analysis strategy fusing several NiEEQ methods should be urgently developed and confirmed by proper clinical trials.

• Membrane Journal
• /
• v.14 no.2
• /
• pp.85-98
• /
• 2004

The situation of technology Predominance and the distribution of core technology were visually mapped thorough the investigation of technical trend during 20 years, which was provided with the analysis of patent information for the artificial skin. Therefore, it was expected that technology mapping by means of multilateral analysis method allowed a good grasp of current technology trend of the artificial skin and the subdivision into nation and a field of research presents the direction of R&D. In the early R&D stage of artificial skin, the preparation technology of filters implantable into the living body including nursing devices, bandages, and dressings or absorbent pads (A6IF-002/10) was on the rise in research field of artificial skin until 1980's. And then the materials technology for coating prostheses (A6l L-027/00)was leading the core technology of artificial skin. Also, Nowadays the fusion technology connected the material technology with the cultivation technology of undifferentiated human or animal cells/tissues including culture media (C l2N-005/00, C 12N-005/06) was highlighted all over the world.

• Geomechanics and Engineering
• /
• v.17 no.5
• /
• pp.421-427
• /
• 2019

Coastal erosion is becoming a significant problem in Greece, Bangladesh, and globally. For the prevention and minimization of damage from coastal erosion, combinations of various structures have been used conventionally. However, most of these methods are expensive. Therefore, creating artificial beachrock using local ureolytic bacteria and the MICP (Microbially Induced Carbonate Precipitation) method can be an alternative for coastal erosion protection, as it is a sustainable and eco-friendly biological ground improvement technique. Most research on MICP has been confined to land ureolytic bacteria and limited attention has been paid to coastal ureolytic bacteria for the measurement of urease activity. Subsequently, their various environmental effects have not been investigated. Therefore, for the successful application of MICP to coastal erosion protection, the type of bacteria, bacterial cell concentration, reaction temperature, cell culture duration, carbonate precipitation trend, pH of the media that controls the activity of the urease enzyme, etc., are evaluated. In this study, the effects of temperature, pH, and culture duration, as well as the trend in carbonate precipitation of coastal ureolytic bacteria isolated from two coastal regions in Greece and Bangladesh, were evaluated. The results showed that urease activity of coastal ureolytic bacteria species relies on some environmental parameters that are very important for successful sand solidification. In future, we aim to apply these findings towards the creation of artificial beachrock in combination with a geotextile tube for coastal erosion protection in Mediterranean countries, Bangladesh, and globally, for bio-mediated soil improvement.

• The Journal of the Convergence on Culture Technology
• /
• v.8 no.6
• /
• pp.873-879
• /
• 2022

In the future, AI and the metaverse are becoming so powerful that their application areas and influences are swallowing up the world. The advertising field is no exception, and it is becoming more important to predict, analyze, and strategize these future changes. In order to study the future change of advertising according to the development of artificial intelligence and metaverse, literature research related to the development of artificial intelligence and metaverse technology and the resulting change in the advertising environment, in-depth interviews with future and advertising experts, and Delphi technique research method I want to study change. First, through this study, we would like to examine the opinions of experts through in-depth interviews on the development of artificial intelligence and metaverse technology and the changes in the advertising sector in the post-coronavirus era of civilizational transformation. In addition, the Delphi technique is used to determine how important the change is by future advertising technology area, future advertising media area, future advertising form area, future advertising effect area, future advertising application area, and future advertising process area, and at what point in the future it will change. In addition, we want to study how the future advertising form will change in detail. Also, based on this, we would like to propose a countermeasure for the advertising industry.