• Korean Journal of Organic Agriculture
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• v.21 no.4
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• pp.737-752
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• 2013

This study was conducted to evaluate the allelopathic effect of aqueous extract of Ganghwa domestic mugwort (Artemisia spp.) on vegetables and its related allelo-chemicals. When the receptor vegetables, such as Chinese cabbage, lettuce, and red radish, were treated with aqueous extract obtained from Sajabalssuk (A. $sp^*I$), Ssajuarissuk (A. $sp^*II$) or Ssajarissuk (A. $sp^*III$), their germination rate, leaf number, plant height, and root length were restricted with increasing concentration of aqueous extract. Allelopathic effect was the highest in radish, than lettuce and Chinese cabbage in order. The growth of topplant were more inhibited then root growth observing in restriction of plant height, root length, and chlorophyll contents. The plant height, the root length of red radish were 53.3 and 61.2% and their fresh weights were 19.8 and 26.4% compared to those of controls, respectively. A. $sp^*III$ showed the highest allelopathic effect among the donor plants. In HPLC analysis, 7 phenol compounds were identified in A. $sp^*I$ and A. $sp^*II$, and, in A. $sp^*III$, and hydroxybenzoic acid and phenylacetic acid were further identified as allelochemicals. It is considered that their plant growths were variously inhibited by the amounts and types of allelochemicals in aqueous extracts. To increase the productivity of farm land after cultivation of mugwort, these results can be useful to select the following field crops.

• Korean Journal of Medicinal Crop Science
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• v.9 no.3
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• pp.181-185
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• 2001

This study was carried out to select chamazulene containing species and to know whether chamazulene content varies depend on individual plants within a population or not. Among five collected mugworts, chamazulene was detected only in Artemisia indica. Chamazulene content in the leaves was higher than that in stems at vegetative stage. Camazulene in inflorescence was not higher than that in leaves even at reproductive stage. Chamazulene was higher in the leaves of the upper part of the plant than those of the middle and lower of the plant at vegetative stage. Chamazulene contents of 50 individuals within a population were varied from 0.01% to 0.11% with mean of 0.045%. About 36 individuals, 72 percent of total individuals examined, had more than 0.03% of chamazulene. There was no difference in chamazulene contents between parents and their clones.

• Journal of Veterinary Clinics
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• v.24 no.2
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• pp.125-129
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• 2007

Artemisia capillaris THUNB is a perennial herb that belongs to the family Compositae spp and the most common plant among the various herbal folk remedies used in treatment of abdominal pain, hepatitis, chronic liver disease, jaundice and coughing in Korea. This experiment was conducted to investigate the inhibitory effects of orally administrated Artemisia capillaris extracts on the pathogenic bacteria in 200 ICR mice. The experimental groups showed inhibitory effects on the bacteria in $1{\sim}3$ days after inoculation. After 21 days of inoculation, no viable bacterial cells appeared in the feces of both experimental groups while they did appear in the control group. The results of these studies indicate Artemisia capillaris extract exhibited excellent antimicrobial and inhibitory effects on the food poisoning pathogenic bacteria; S. enteritidis, E. coli O157:H7, L. monocytogenes and S. aureus.

• Journal of Microbiology and Biotechnology
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• v.22 no.7
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• pp.975-980
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• 2012

Monacolin K-enriched ganghwayakssuk (Artemisia princeps Pamp.) was developed by fermentation with Monascus sp. Among the 15 Monascus spp. isolated previously from Monascus fermentation products, Monascus pilosus KMU108 produced 2,219 mg/kg of monacolin K during ganghwayakssuk fermentation with no detectable citrinin. The optimum concentrations of ganghwayakssuk and glucose determined from the response surface methodology (RSM) design were 2.2% and 3.8%, respectively. By applying these conditions, the monacolin K productivity was increased to 3,007 mg/kg after 15 days of fermentation. On the other hand, other characteristics such as the total content of flavonoids and phenolic compounds, and the antioxidant activity were relatively unchanged. Therefore, Monascus-fermented ganghwayakssuk is an excellent biomaterial for the development of functional foods because of its high level of monacolin K, known to lower cholesterol levels.

• Journal of Veterinary Clinics
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• v.24 no.2
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• pp.130-136
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• 2007

Artemisia capillaris THUNB is a perennial herb that belongs to the family Compositae spp and the most common plant among the various herbal folk remedies used in treatment of abdominal pain, hepatitis, chronic liver disease, jaundice and coughing in Korea. In this study, antimicrobial effects of Artemisia capillaris extracts on the food poisoning bacteria were investigated for further clinical application, which is an alternative for the use of antibiotics and their unexpected resistance. Artemisia capillaris extract using ethyl acetate showed the highest antimicrobial effects on S. enteritidis, E. coli O157 : H7, L. monocytogenes and S. aureus. The chloroform extract showed strong effects on all kinds of bacteria; whereas ethanol and methanol extracts showed weaker effects. Finally, ether and water extracts showed the weakest effects under the same conditions. The minimum inhibitory concentration (MIC) of ethyl acetate extract was 1 mg/mL for E. coli O157 : H7 and L. monocytogenes, and 2 mg/mL for S. enteritidis and S. aureus. The inhibitory effects on all the bacteria continued for 12 hours after incubation using 20 mg/mL and 30 mg/mL of ethyl acetate extract. The inhibitory effects continued maximally for 72 hours. The results of these studies indicate Artemisia capillaris extract exhibited excellent antimicrobial and inhibitory effects on the food poisoning pathogenic bacteria; S. enteritidis, E. coli O157 : H7, L. monocytogenes and S. aureus.

• Korean Journal of Medicinal Crop Science
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• v.2 no.3
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• pp.205-210
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• 1994

Artemisw spp. have been known as natural health foods from ancient time and recently, Artemisia spp. have been developed as a preference food. In the paper Artemisia spp. collected from 28 local areas in 1993 were growth in condition shaded selected some suitable species for summer cultivation. Growth characteristics and chemical components of shade-cultivated were summerized by follows. 1. The volume per $m^2$ was 3.925g collected in Suncheon. Jeonnam which was most abundant, and 713g in Okcheon. Chungbuk was little. and there was difference at 5.6times. The volumes per $m^2$ collected in species were plentiful of 3,420g in Hwanghaessak, 3,173g in Meongcheonssuk and 3.l48g Siljebissuk. But there were little of 2,093g in Panssuk and 2,078g in Keunjebissuk. 2. Hwanghaessk, Siljebissuk and Meongcheonssuk were good seasoning, and leaf color was light green. and also comodities was show a good result. 3. Hwanghaessuk, which was excellent quality and quantity, was include many iron and magnesium, but content of copper was low. Meongcheonssuk and pangssuk were excellent in comodities. and content of protein was abundant, but contents of catcalcium, iron, and zink were low. 4. Hwanghaessuk and Siljebissuk were a great many quantity and taste, leaf color, quality and comodity were excellent. Therefore Hwanghaessuk and Siljebissuk were most suitable cultivated in shading the light.

• Journal of Veterinary Clinics
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• v.24 no.3
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• pp.367-371
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• 2007

Artemisia capillaris THUNB is a perennial herb that belongs to the family Compositae spp and the most common plant among the various herbal folk remedies used in treatment of abdominal pain, hepatitis, chronic liver disease, jaundice and coughing in Korea. This experiment was conducted to investigate cytotoxic effects of Artemisia capillaris extracts on the Hepa-1c1c7 and Sarcoma 180 cancer cells on in vitro experimental tests. On in vitro tests using MTT assay and SRB assay, the extracts showed prominent cytotoxic effects on the two kinds of cancer cell lines, respectively. Antihumor effects appeared in the concentration of over $250{\mu}g/mL$ of both ethanol and ethyl acetate extract, over $500{\mu}g/mL$ of methanol extract, over $5000{\mu}g/mL$ in water extract and over 50% cytotoxicity on the Hepa-1c1c7 and Sarcoma 180. The results suggest that Artemisia capillaris extracts have prominent cyotoxic effects on the cancer cell lines Hepa-1c1c7 and Sarcoma 180.

• Journal of Veterinary Clinics
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• v.24 no.3
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• pp.372-378
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• 2007

The Artemisia capillaris THUNB is a perennial herb that belongs to the family Compositae spp and probably the most common plant among the various herbal folk remedies being used in the treatment of abdominal pain hepatitis chronic liver disease, jaundice and coughing in Korea. Recently the biological and pharmacological actions of herb have been studied well such as antibacterial, antidiabetic and antitumor activities. This experiment was conducted to investigate antitumor and immunomodulatory effects of Artemisia capillarix extracts against Hepa-1c1c7 and Sarcoma 180 cancer cells on in vivo experimental tests. On in vivo experimental tests using 280 ICR mice the gain of body weight in the control-group mice bearing Sarcoma 180 ascites tumor was 1.5 times more than that of the normal-group mice after 33 days. However, the gain of body weight in all experimental groups administered with Artemisia capillaris extracts was significantly lower than that of the control-group mice (P<0.05). The mean survival times of mice administered with Artemisia capillaris extracts of 25 and 100 mg/kg for 28 days were shown to be 25.39% and 15.39% longer than that of the control-group mice injected with saline (P<0.05). Artemisia capillaris extracts showed the highest tumor inhibition effects, which were 42.4% and 27.2% when intraperitoneally injected with doses of 25 and 100 mg/kg once a day for 28 days in inoculated ICR mice with Sarcoma 180 solid tumor cells (P<0.05). The results suggest that Artemisia capillaris methanol extracts have prominent antitumor effects on the cancer cell lines Hepa-1c1c7 and Sarcoma 180.

• Journal of Veterinary Clinics
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• v.26 no.5
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• pp.408-412
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• 2009

The Artemisia capillaris THUNB is a perennial herb that belongs to the family Compositae spp. and probably the most common plant among the various herbal folk remedies being used in the treatment of abdominal pain, hepatitis, chronic liver disease, jaundice and coughing in Korea. This experiment was conducted to investigate the effects of Artemisia capillaris extracts on the amounts of splenocytes-derived cytokine ($TNF-{\alpha},\;IL-1{\beta}$ and IL-10). In in vivo experimental tests using 210 ICR mice with Hepa-1c1c7 or sarcoma 180 cancer line, splenocytes derived cytokine contents were significantly (p < 0.05) reduced in the Hepa-1c1c7 and Sarcoma 180 inoculated vehicle controls, HP and SP, compared to those of the intact vehicle control on both the $28^{th}$ day and the $42^{nd}$ day, respectively. However, these decreases of $TNF-{\alpha},\;IL-1{\beta}$ and IL-10 levels induced by tumor inoculations were significantly (p < 0.01, p < 0.05) inhibited by mACH (Artemisia capillaris methanol extracts) treatment regardless of the type of experiments and tumor cells inoculated. The results suggest that Artemisia capillaris methanol extracts have prominent anti-inflammation effects on the cancer cell lines Hepa-1c1c7 and Sarcoma 180.

• Journal of Veterinary Clinics
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• v.26 no.1
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• pp.1-7
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• 2009

The Artemisia capillaris THUNB is a perennial herb that belongs to the family Compositae spp and probably the most common plant among the various herbal folk remedies being used in the treatment of abdominal pain, hepatitis, chronic liver disease, jaundice and coughing in Korea. Recently the biological and pharmacological actions of herb have been studied well such as antibacterial, antidiabetic and antitumor activities. This experiment was conducted to investigate antitumor and immunomodulatory effects of Artemisia capillaris extracts against Hepa-1c1c7 and Sarcoma 180 cancer cells in in vivo experimental tests. In in vivo experimental tests using 210 ICR mice, based on flow cytometry, CD3+, CD4+, CD8+ and TNF-${\alpha}+$ splenocytes were significantly (p<0.05) reduced in the Hepa-1c1c7 and Sarcoma 180 inoculated vehicle controls, HP and SP, compared to those of the intact vehicle control on both the $28^{th}$ day and the $42^{nd}$ day, respectively. These decreases of CD3+, CD4+, CD8+ and TNF-${\alpha}+$ cells induced by tumor inoculations were significantly (p<0.05) inhibited by mACH treatment regardless of the type of experiments and tumor cells inoculated. The results suggest that Artemisia capillaris methanol extracts have prominent antitumor effects on the cancer cell lines Hepa-1c1c7 and Sarcoma 180.