• 농업과학연구
• /
• 제23권1호
• /
• pp.99-107
• /
• 1996

Eighteen nuclear probes were used to examine RFLP(restriction fragment length polymorphism) between six species of Artemisia spp. of Korea. Total DNA from six different species of Artemisia was separately cut with three restrict enzymes. The PstI enzyme was showed to reduce the variation of polymorphisms than the other two enzymes(EcoRl and BamHI). The genetic variation of polymorphism was similar between the Dhewegiki-ssug and Cham-ssug. RAPD analysis was applied to the same six species of Artemisia spp. in order to assess the degree of DNA polymorphism within the Artemisia genus. Six species of Artemisia were evaluated for variation using a set of 11 random 10-mer primers. Nine out of the eleven primers revealed scorable polymorphisms between six species of Artemisia spp. Genetic distances between each of the species were calculated and cluster analysis was used to generate a dendrogram showing phylogenetic relationships between them This result indicates that molecular markers will be more usable in intraspecific study of Artemisia spp. than isoenzyme markers.

• 한국유기농업학회지
• /
• 제20권4호
• /
• pp.589-605
• /
• 2012

This study was conducted to identify allelopathic effect of Ganghwa domestic Artemisia spp., named Sajabalssuk and Ssajuarissuk, for various receptor plants including clover (Trifolium repens L.), alfalfa (Medicago sativa L.), lawn grass (Zoysia japonica Steud.), dandelion (Taraxacum platycarpum Dahlst.), and dahurianpatrinia (Patrinia scabiosaefolia Fisch. ex Trevir). Receptor plants were treated with the aqueous and essential oil extract of Artemisia plants. In consequence, their allelopathic effects were evaluated by measuring seed germination rates, seedling growth, and dry weights of the receptor plants. The seed germination and seedling growth of the receptor plants were inhibited by all treatments of both aqueous and essential oil extracts of the Artemisia plants, and, in addition, the inhibitory effects were increased according to the higher concentration. Among the donor plants, A. $sp.^*III$ showed most effective allelopathic effect. Comparing the alleopathic effect among the receptor plants, seed germination was most inhibited in lawn grass while inhibitory effect of seedling growth was comparatively higher in dandelion. Although inhibitory effects were comparatively lower, the allelopathic effects of Artemisia plants were identified in clover and alfalfa since the seedling growth of these plants were inhibited more than 70%. Thus, in result, Ganghwa domestic Artemisia spp. could be possibly used for weed control since natural products of the plants showed inhibitory effects on seed germination and seedling growth of various receptor plants.

• KSBB Journal
• /
• 제20권3호
• /
• pp.228-232
• /
• 2005

본 연구에서는 우리나라 민속약이나 한방의 약재로 널리 사용되어 온 참쑥 (Artemisia mongolica fishcher)으로부터 추출한 쑥 추출물이 포도상구균 (Staphylococcu aurues)과 유산균 (Lactobacillus spp.)에 대해 선택적 저해 효과를 갖는지 조사하였다. 우선 항균 활성 실험을 통해 포도상구균을 저해하는 우수한 항균력을 가진 유산균 7균주를 선별하였다. 그리고 쑥을 추출한 용매의 종류, 농도 그리고 추출시간을 달리하여 쑥 추출물의 최적 농도 조건을 결정하였다. 생존율을 측정하기 위해 배양액에 각각 3일 동안 용매 $100\%$ (아세톤, 에탄올, 메탄올)로 추출한 쑥 추출물을 $17\%$ 첨가하였고, 이때 항균 활성 있는 유산균 KLB 224은 배양 48시간까지 생존하였다. 그러나 포도상구균은 동일한 조건에서 모두 사멸되었다. 또한 주사전자현미경 관찰을 통해 쑥 추출물이 세포 형태에 미치는 영향을 확인하였다. 그 결과, 포도상구균은 세포 형태가 심하게 손상되었지만 유산균은 저해를 받지 않고 세포형태를 유지함을 관찰하였다.

• The Korean Journal of Ecology
• /
• 제18권2호
• /
• pp.275-283
• /
• 1995

Seral changes in floristic composition during abandoned field succession after shifting cultivation was investigated in eastern Kangwon-Do, Korea. According to the DCA ordination based on the floristic composition, sere of the dominant species was shown as follows: Digitaria sanguinalis, Persicaria spp., Commelina communis etc. in the annual stage (0∼1 years); Erigeron spp., Artemisia spp., Rubus crataegus etc. in the Erigeron-Artemisia stage (2∼6 years); Pinus densiflora, Salix spp., Miscanthus sinensis, Arundinella hirta etc. in the shrub - earlier tree stage (10∼25 years); P. densiflora, Quercus mongolica, Spodiopogon sibiricus etc. in the mid-tree stage (25∼50 years); Q. mongolica, Tripterigium regelii, Lespedeza maximowiczii, Carex siderosticta etc. in the latter tree stage (50∼80 years). In mid-tree stage, size-frequency distribution of P. densiflora was plotted as a platycurtic curve and that of Q. mongolica as a reverse J-shaped curve, which meant P. densiflora was thinned through interspecific competition and Q. mongolica was regenerated by itself in the later tree stage.

• 한국작물학회지
• /
• 제51권spc1호
• /
• pp.209-214
• /
• 2006

전국의 산과 들의 생육조건이 다양한 지역에서 생육한 약쑥, 뺑쑥, 참쑥, 제비쑥, 물쑥, 황해쑥, 더위지기, 가는잎쑥 등에 대하여 수집한 쑥 100계통을 실험에 이용하여 항산화력과 항산화 성분 함량을 비교 분석하였다. 합성 항산화제인 BHT와 비교 실험한 지질과산화 억제력을 살펴보았을때 모든 쑥 시료에서 BHT 200 ppm과 유사한 억제력을 나타내어 그 우수성을 확인할 수 있었다. 쑥 수집종 100계통의 AEAC, EDA, 총페놀함량, 플라보노이드 등을 분석한 결과 쑥 수집종의 총 페놀화합물 함량은 $156{\sim}1,767mg/100g$ 범위로 최대 10배의 함량변이를 보였는데, 총 페놀화합물의 함량이 900mg/100g 이상인 수집종은 총 20 개체였다. 플라보노이드 함량도 종간, 수집지역 간에 편차가 컸는데 $82.9{\sim}852.2mg/100g$ 범위로 변이의 폭이 컸다. 전자공여능의 경우 $13.4{\sim}95.0%$의 범위로 다양하였으며 전자공여능이 90% 이상인 수집종은 총 23개체였다. ABTS와 DPPH 라디칼을 이용한 에탄을 추출물의 항산화력 측정 결과 총 페놀화합물 함량이 높았던 대부분의 시료에서 높은 라디칼 제거능을 나타내었다. 본 연구 결과 항산화력이 우수하며 생육 특성 및 수량성이 우수한 AC-60, AC-67, AC-77을 우수 계통으로 선발하였다.

• 한국약용작물학회지
• /
• 제13권6호
• /
• pp.278-286
• /
• 2005

쑥 수집종 80개를 대상으로 형태적 형질과 RAPD 분석을 하였고, 유전적 다양성을 이용하여 유연관계를 분석하고 이를 기초로 품종군을 분류하였다. 주요 형태적 형질을 이용하여 쑥 수집종에 대한 군집분석 결과, 군집간의 치대거리 0.82를 기준으로 하여 분류하였을 때 5개 군으로 분류하였는데, I군에 10개 (15%), II군에 30개 (37.5%), III군에 20개 (25%), IV군에 3개 (4%), V군에 4개 (5%)를 나타내었다. RAPD분석에 이용한 10-mer primer 에 대하여 98개의 밴드를 얻었고, 그 중 다형성을 보인 밴드는 68개로 69%였는데, 선발된 Primer에서 증폭된 밴드 수는 $8{\sim}11$개로 다양하였으며, 평균 9.8개였다. RAPD에 의한 군집 분석에서 유연계수 0.63을 기준으로 하여 구분한 결과 6개의 군으로 분류되었다. I, II군이 각각 전체의 34%, 36%를 차지하여 가장 큰 군으로 분류되었고, 나머지 $III{\sim}V$군은 모두 소군으로 15%가 속하였다. 특히 I군에는 약쑥이 많았고, II군에는 뺑쑥이 많이 분포하였다.

• 생약학회지
• /
• 제8권4호
• /
• pp.155-161
• /
• 1977

Pollinosis usually caused by the airborne pollen grains, is an allergical disease which causes snivel and sneeze. The author has studied on airborne pollen grains collected at Chong-ro district in Seoul about 15m from April 1975 to March 1976. The obtained grains were 4 spp. of Gymnospermae, 19 spp. of Dicotyledoneae and 10 spp. of Monocotyledoneae. The tree season ranged from March to June, Alnus, Pinus and Acer were prevalent. The grass season ranged from May to October, Gramineae were prevalent. The weed season ranged from August, Artemisia were prevalent and ragweed was found.

• Biomolecules & Therapeutics
• /
• 제4권4호
• /
• pp.354-363
• /
• 1996

This study was conducted to investigate the repeated dose toxicity of DA-9601, an antiulcer agent of Artemisia app. extract, in rats. DA-9601 was administered orally once a day for 4 weeks to 10 males and 10 females per group at doses of 0(vehicle control), 125, 500 or 2000 mg/kg/day. Throughout the study, no treatment-related deaths and clinical signs were observed. In female rats receiving 125 mg/kg of DA-9601, water consumption increased slightly on day 4, 11 and 25. Hematological examination showed a decrease of MCV and an increase of PLT in male rats at the doses of 500 and 2000 mg/kg groups. Blood biochemistry revealed slight decreases of cholesterol, BUN and Na in male rats and decreases of total bilirubin and creatinine and slight increases of globulin and Cl in female rats. The organ weights at the end of 4 weeks showed slight changes in some organs of treated groups. But, all these changes were not considered to be of toxicological importance, because they did not show dose-response relationship and relevance to gross and microscopic findings. Histopathologically, abnormal treatment-related changes were not observed in any organ and target organs were not detected. On the basis of these results, the NOAEL(no-observed-adverse-effect level) of DA-9601 was estimated to be more than 2000 mg/kg/day under the conditions tested.

• Journal of Applied Biological Chemistry
• /
• 제66권
• /
• pp.244-249
• /
• 2023

Artemisia keiskeana and A. stolonifera are plants of the genus Artemisia, distributed in various regions, especially China and Korea. They are renowned as medicinal plants with biological and pharmacological activities. Fraxidin, isofraxidin, and daphnoretin are coumarins present in Artemisia spp.; however, research on them is limited. Therefore, this study was carried out to quantify the content of these compounds in the aerial parts of A. keiskeana and A. stolonifera in different regions in Korea. High-performance liquid chromatography was performed with a photodiode array detector and a reverse-phase INNO column. A. stolonifera only contained fraxidin with the highest amount found in Yongmun commune. A. keiskeana cultivation in Soyang commune gave the highest fraxidin and daphnoretin content. However, isofraxidin was not present in all samples. The findings suggest that the concentrations of the three compounds may differ depending on the growth site and provide a foundation for future studies.

• 한국약용작물학회:학술대회논문집
• /
• 한국약용작물학회 2008년도 추계학술발표회
• /
• pp.479-480
• /
• 2008