• Journal of Microbiology and Biotechnology
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• v.14 no.3
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• pp.593-598
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• 2004

The arsenical resistance (ars) operon was cloned from a 67-kilobase pair (kb) plasmid, which was previously shown to be responsible for arsenic salts resistance in K. oxytoca D12. When plasmid pAE48, carrying the ars operon, was transformed into E. coli, transformed cells displayed enhanced survival in the presence of 4 mM arsenite, 50 mM arsenate, or 0.4 mM antimonite. The nucleotide sequence of the 5.6-kb fragment encoding arsenical resistance revealed five open reading frames (ORFs), which were predicted to encode polypeptides of 12.8 (arsR), 13.4 (arsD), 62.6 (arsA), 45 (arsB), and 16.7 (arse) kilodaltons (kDa). Each ORF was preceded by a ribosome binding site. A putative promoter-like sequence was identified upstream of arsR, and a possible termination site was found downstream of arsC. When the deduced amino acid sequences of the K. oxytoca Dl2 Ars proteins were compared with the amino acid sequences of the E. coli R773 Ars proteins, a significant amino acid similarity was observed (87.9％ for ArsR, 89.2％ for ArsD, 83.2％ for ArsA, 92.6％ for ArsB, and 91.3％ for ArsC), suggesting an evolutionary relationship of the ars genes of E. coli plasmid R773 and K. oxytoca Dl2.

• Journal of Microbiology and Biotechnology
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• v.17 no.5
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• pp.812-821
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• 2007

The ecosystems of certain abandoned mines contain arsenic-resistant bacteria capable of performing detoxification when an ars gene is present in the bacterial genome. The ars gene has already been isolated from Pseudomonas putida and identified as a member of the membrane transport regulatory deoxyribonucleic acid family. The arsenite-oxidizing bacterial strains isolated in the present study were found to grow in the presence of 66.7 mM sodium arsenate($V;\;Na_2HAsO_4{\cdot}7H_2O$), yet experienced inhibited growth when the sodium arsenite($III;\;NaAsO_2$) concentration was higher than 26 mM. Batch experiment results showed that Pseudomonas putida strain OS-5 completely oxidized 1 mM of As(III) to As(V) within 35 h. An arsB gene encoding a membrane transport regulatory protein was observed in arsenite-oxidizing Pseudomonas putida strain OS-5, whereas arsB, arsH, and arrA were detected in strain OS-19, arsD and arsB were isolated from strain RW-18, and arsR, arsD, and arsB were found in E. coli strain OS-80. The leader gene of arsR, -arsD, was observed in a weak acid position. Thus, for bacteria exposed to weak acidity, the ars system may cause changes to the ecosystems of As-contaminated mines. Accordingly, the present results suggest that arsR, arsD, arsAB, arsA, arsB, arsC, arsH, arrA, arrB, aoxA, aoxB, aoxC, aoxD, aroA, and aroB may be useful for arsenite-oxidizing bacteria in abandoned arsenic-contaminated mines.

• Proceedings of the Korean Society of Crop Science Conference
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• 2006.04a
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• pp.292-293
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• 2006

• 한국IT서비스학회:학술대회논문집
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• 2009.11a
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• pp.409-412
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• 2009

고객과 접하는 통화수단으로 VRS의 필요성이 대두되고 있다. 기존 ARS를 통해 서비스를 하고 있는 사업체의 경우 VRS는 또하나의 새로운 시스템 도입이라는 점과 기존 고객에게 접근하는데 있어서 큰 변화를 느끼지 않으면서 보다 쉽고, 편리하게 다가 갈 수 있어야 한다는 점에서 고민을 안겨준다. 본 논문에서는 기존 ARS 시스템을 이용하여 쉽게 VRS를 적용할 수 있는 방법을 제시하고, 향후 VRS의 발전방향을 제시한다.

• Speech Sciences
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• v.8 no.2
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• pp.61-71
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• 2001

Speech material, which is collected from ARS(Automatic Response System), was analyzed and classified into disease and non-disease state. The material include 11 different kinds of diseases. Along with ARS speech, DAT(Digital Audio Tape) speech is collected in parallel to give the bench mark. To analyze speech material, analysis tools, which is developed local laboratory, are used to provide an improved and robust performance to the obtained parameters. To classify speech into disease and non-disease class, multi-layered neural network was used. Three different combinations of 3, 6, 12 parameters are tested to obtain the proper network size and to find the best performance. From the experiment, the classification rate of 92.5% was obtained.

• Journal of Technology Innovation
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• v.20 no.3
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• pp.181-198
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• 2012

Gyeonggi-Do launched the 'Achievement Royalty System(ARS)' in 2011 to promote the refund of excess profits from those firms to local system. The purpose of this study aims to analyze the effect of new royalty system in the public R&D program. From the analysis, commercially successful firms appear to have maintained the same level of innovation incentives even after introduction of ARS. It can be explained that they have given a priority in taking part in the next R&D projects as well as a benefit of systematic supports in technology commercialization and marketing. It is, therefore, fully expected that the policy makers can make ARS an additional funding source in the period of decreasing S&T budget, and have a better chance to gather evidences of successful policy practices to the firms. However, the institutional improvements are required to develop the ARS, which include the incentives of ARS payment and the lower total royalty expectation through the reduction of fix-payment ratio and the exemption of ARS.

• Proceedings of the Korean Society of Crop Science Conference
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• 2006.04a
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• pp.232-233
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• 2006

• Journal of Microbiology and Biotechnology
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• v.3 no.1
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• pp.6-11
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• 1993

An autonomously replicating sequence (Kf-ARS1) of Kluyveromyces fragilis was cloned from the genomic library which was constructed using pHN134 as a cloning vector to make a new host-vector system for the production of heterologous protein from K. fragilis as a host. The cloning vector pHN134 was composed of $Km^r, Ap^r$ and multiple cloning site in LacZ . A clone carrying Kf-ARS1 was isolated and the recombinant plasmid was designated as pIKD102. The cloned fragment was 2.3 kb (EcoRI/EcoRI) in length. Subcloning experiment showed that the region for ARS activity was 1.5 kb (SalI/EcoRI) fragment. It was shown that the Kf-ARS1 was active in Saccharomyces cerevisiae and Kluyveromyces fragilis.

• Proceedings of the Korea Society of Environmental Toocicology Conference
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• 2001.05a
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• pp.141-141
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• 2001

The arsenic resistance operon from pMH12 in Klebsiella oxytoca contains two regulatory genes. The first open reading frame for arsR extend up to 348 bp and has a translational product corresponding to a protein of 116 amino acid residue polypeptide with a molecular mass of 13 kDa. And the second ORF for arsD extend up to 360 bp and express a protein of 120 amino anid residue polypeptide with 13kDa. (omitted)

• Proceedings of the Zoological Society Korea Conference
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• 1999.10b
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• pp.282.1-282
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• 1999

No Abstract, See Full Text