• Journal of Microbiology and Biotechnology
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• v.6 no.5
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• pp.331-335
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• 1996

The second $\beta$-Xylosidase gene (xylB) from Bacillus stearothermophilus was isolated from the genomic library, cloned into pBR322, and subsequently transferred into Escherichia coli HB101. Six out of 10, 000 transformants were selected from the selective LB medium supplemented with p-nitrophenyl-$\alpha$-L-arabinofuranoside (pNPAf) and ampicillin ($50\mu g$/ml) based on their ability to form a yellow ring around the colony. One of the clones was found to harbor the recombinant plasmid with 5.0 kb foreign DNA, which was identical to the $\alpha$-L-arabinofuranosidase gene (arfI) previously cloned in this lab, while the other five had 3.5 kb of the foreign DNA. Southern blotting experiments confirmed that the 3.5 kb insert DNA was from B. stearothermophilus chromosomal DNA. A zymogram with 4-methylumbelliferyl-$\alpha$-L-arabinofuranoside as the enzyme substrate revealed that the cloned gene product was one of the mutiple $\alpha$-L-arabinofuranosidases produced by B. stearothermophilus. Unlike the arfI gene product, the product of the gene on the insert DNA (xylB) showed an activity not only on pNPAf but also on oNPX suggesting that the cloned gene product could be a bifunctional enzyme having both $\alpha$-L-arabinofuranosidase and $\beta$-xylosidase activities.

• Journal of Microbiology
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• v.39 no.3
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• pp.236-239
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• 2001

Group A Streptococcus strain ST4529 is a provisional new ems type which has been recently reported in Malaysia (Jomal, et al. 1999. Energ. Infect . Dis. 5,10-14). This strain was found to be opacity factor (OF) negative with a Tl phenotype. Usually, OF negative strains with T1 phenotypes are associated with acute rheumatic fever. However, strain ST4529 was isolated from the blood of a patient with septicemia. Comparison of the deduced amino acid sequence of the mature hypervariable N-terminus of ST4529 showed only 43% identity with that of M5, the closest matched OF negative strain with a T1 phenotype. Thus, ST4529 most probably encodes a new serospecifically unique M protein which is associated with septicemia rather than pharyngitis infections. The strains with these phenotypes are very important because their sequences should be considered for developing any anti-streptococcal vaccines.

• Biomolecules & Therapeutics
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• v.25 no.1
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• pp.26-43
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• 2017

Endocytosis is a process by which cells absorb extracellular materials via the inward budding of vesicles formed from the plasma membrane. Receptor-mediated endocytosis is a highly selective process where receptors with specific binding sites for extracellular molecules internalize via vesicles. G protein-coupled receptors (GPCRs) are the largest single family of plasma-membrane receptors with more than 1000 family members. But the molecular mechanisms involved in the regulation of GPCRs are believed to be highly conserved. For example, receptor phosphorylation in collaboration with ${\beta}$-arrestins plays major roles in desensitization and endocytosis of most GPCRs. Nevertheless, a number of subsequent studies showed that GPCR regulation, such as that by endocytosis, occurs through various pathways with a multitude of cellular components and processes. This review focused on i) functional interactions between homologous and heterologous pathways, ii) methodologies applied for determining receptor endocytosis, iii) experimental tools to determine specific endocytic routes, iv) roles of small guanosine triphosphate-binding proteins in GPCR endocytosis, and v) role of post-translational modification of the receptors in endocytosis.

• Journal of KSNVE
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• v.7 no.5
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• pp.811-817
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• 1997

This paper is concerned with the active vibration controller design for the grid structure based on the positive position feedback (PPF) and the strain rate feedback (SRF) control. A new control methodology by the combination of the PPF and SRF control can suppress all the modes of the structure theoretically and can be easily implemented with analog circuits. The underlying concept for the design of the new controller is that the SRF controller stabilizes the modes higher than the second mode and the PPF controller stabilizes the fundamental mode which is destabilized by the SRF controller. In order for the new controller to be implemented succesfully, the collocated control is necessary. To this end, the piezoceramic sensor and actuator are located as close as possible, thus realizing the nearly collocated control. The combined PPF and ARF controller proves its effectiveness by experiments.

• Journal of Biomedical Engineering Research
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• v.12 no.2
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• pp.79-88
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• 1991

Semi-transparent volume rendering technique can provide 3-D visualization well by voxel level Processing and alleviate segmentation arf, ifacts compared wish the surface rendering technique. In this Paper, we consider several new schemes which can improve she Perform ance of volume rendering. A directional interpolation method is proposed to reduce the artifact due to the anisotrophic resolution in X-ray CT data. The computation time for rendering is shortened by using the depth information of the 3-D object. And also, we reduce the quantization artifacts in the rendering by introducing the opacity-dependent sampling interval to sampling in ray-tracing.

• Fibers and Polymers
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• v.1 no.1
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• pp.59-65
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• 2000

In order to investigate psychoacoustic characteristics of fibers, and to compare them with sound physical parameters, each sound of 25 different fabrics consisted of a single fiber such as wool, cotton, silk, polyester, and nylon was recorded. Sounds of specimens were transformed into critical band diagram and psychoacoustic characteristics including loudness and sharpness for each sound were calculated based on Zwicker＇s models. Physical parameters such as the level pressure of total sound (LPT), level ranges (ΔL), frequency differences (Δf), AR coefficients (ARC, ARF, ARE) were obtained in fast fourier transform (FFT) spectrum. Nylon taffeta showed higher values for loudness than 2.5 sone corresponding to human low conversation, while most silk fibers generated less louder showing lower values for loudness than 1.0 sone. Wool fibers had higher loudness mean value than that of cotton, while the two fibers didn't differ in LPT. Loudness showed high positive correlation coefficients with both LPT and ARC. Sharpness values were higher for wool fiber group than other fibers. Sharpness was not concerned with loudness, LPT, and ARC, but the fabrics with higher values for sharpness tended to show higher ΔL.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.31 no.2A
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• pp.154-167
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• 2006

Today's IEEE 802.11 WLANs(Wireless LANs) provide multiple transmission rates so that different rates can be exploited in an adaptive manner depending on the underlying channel condition in order to maximize the system performance. Many rate adaptation schemes have been proposed so far while most(if not all) of the commercial devices implement a simple open-loop rate adaptation scheme(i.e., without feedback from the receiver), called ARF(Automatic Rate Fallback) due to its simplicity. A key problem with such open-loop rate adaptation schemes is that they do not consider the collision effect, and hence, malfunction severely when many transmission failures are due to collisions. In this paper, we propose a novel rate-adaptation scheme, called CARA(Collision-Aware Rate Adaptation). The key idea of CARA is that the transmitter station combines adaptively the Request-to-Send/Clear-to-Send(RTS/CTS) exchange with the Clear Channel Assessment(CCA) functionality to differentiate frame collisions from frame transmission failures cause by channel errors. Therefore, compared with other open-loop rate adaptation schemes, CATA is more likely to make the correct rate adaptation decisions. Through extensive simulation runs, we evaluate our proposed scheme to show that our scheme yields significantly higher throughput performance than the existing schemes in both static and time-varying fading channel environments.

• Journal of Veterinary Clinics
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• v.30 no.1
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• pp.57-60
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• 2013

Ingestion of grapes or raisins has been reported to the occurrence of acute renal failure (ARF) in dogs, although the mechanism remains undetermined. The prognosis often depends on the severity or clinical course of the disease at the time of presentation and is poor if the dog becomes anuric phase. To explore the characteristics and outcome of ARF caused by grape or raisin poisoning, sequentially collected data, from 2005 to 2008, of the Veterinary Medical Teaching Hospital at the Kangwon National University for clinical evaluation were retrospectively analyzed. Of the 11 clinically affected dogs, 4 cases made a full recovery, 3 died and 4 were euthanized. All but one case (raisin ingestion) had a history of grape exposure, but the exact quantity of fruit ingested was not known. The female dogs accounted for 72.7% (8 cases). Overall, the mean age was 5.3 years (range 0.2-11.3 years), and the mean body weight was 4.1 kg (range 1.4-13 kg). The average duration of hospital stay was 7.1 days (range 2-22 days). Vomiting and anorexia was reported in all dogs. Diarrhea (4 cases), oliguria (5 cases), and anuria (4 cases) with or without isosthenuria were also reported. Five dogs of 11 had mild to moderate anemia, with a decrease in packed cell volume and hemoglobin. All dogs had elevations in serum phosphorous, creatinine, and blood urea nitrogen values, but calcium values were variable; 2 dogs with hypocalcemia, 2 dogs with hypercalcemia, and the remaining 7 cases within reference interval. Dogs (n = 8) with measured on blood gas parameters had metabolic acidosis. In addition, higher serum enzyme activities were observed; amylase in 8 (72.7%) dogs, alkaline phosphatase in 7 (63.6%) dogs, and alanine aminotransferase in 5 (45.5%) dogs. Non-survived dogs revealed lower counts of platelet and lymphocyte subpopulation, as compared to the survived dogs.

• Journal of the Korean Society of Radiology
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• v.11 no.3
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• pp.123-129
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• 2017

Compton suppression apparatus using the Compton scattering response, by inhibiting part of the spectrum Compton continuum Compton continuum in the area of the peak analysis of the gamma rays that enables a clearer device. In order to find out the geometry structure of high-purity germanium detector(HPGe) -NaI(TI) and to optimize the effect of movement, Monte Carlo simulation was used to grasp the behavioral characteristics of Compton suppression and compare several layout structures. And applied to the cylinder beaker used for the environmental measurement by using the efficiency according to the distance. For the low-energy source such as 81 keV, the Compton continuum is scarcely developed and the suppression effect is also insignificant because the scattering cross-section of the Compton effect is relatively low. In the spectrum for the remaining energy, it can be seen that the Compton continuum part is suppressed in a certain energy range. Compton suppression effect was not significantly different from positional shift. average reduction factor(ARF) value was about 1.08 for 81 keV and about 1.23 for 1332.4keV energy at the highest value. It can be seen that suppression over the Compton continuum region of the energy spectrum is a more appropriate arrangement. Therefore, it can be applied to various environmental sample measurement through optimized structure.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.34 no.4
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• pp.370-377
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• 2001

Flounder brain cytosol contains protein inhibitors that markedly inhibit the activity of partially purified brain membrane phospholipase D (PLD) which is dependent on phosphatidylinositol 4,5-bisphosphate ($PIP_2$) but insensitive to ADP-ribosylation factor (ARF), The PLD inhibitors have been enriched through several chromatographic steps and characterized with respect to size and mechanism of inhibition. Sequential chromatography of the brain cytosol yielded six inhibitor fractions, Two (IIA and IIB) of six inhibitor fractions showed the $PIP_2$-phosphatase activities. IIA was identified as synaptojanin, a nerve terminal protein that has known to be a member of the inositolpolyphosphate 5-phosphatase family, by immunoblot analysis. IIB showed an apparent molecular mass of 158 kDa by Superose 12 gel filtration chromatography and was immunologically distinct from synaptojanin. IIB hydrolyzed $PIP_2$, yielding only phosphatidylinositol phosphate (PIP) as product, suggesting that IIB hydrolyzes only one phosphate from either the 4- or 5-position of PI (4,5)$P_2$. These studies demonstrate that the existence of multiple $PIP_2$-phosphatases have been implicated in the negative regulation of $PIP_2$-dependent PLD activity within flounder brain.