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Analysis of Antioxidant Activity and Serotonin Derivatives in Safflower (Carthamus tinctorius L.) Germplasm Collected from Five Countries (국외 수집 홍화 유전자원의 항산화 활성 및 세로토닌 유도체 함량 분석)

  • Jung, Yi Jin;Assefa, Awraris Derbie;Lee, Jae Eun;Lee, Ho Sun;Rhee, Ju Hee;Sung, Jung Sook
    • Korean Journal of Plant Resources
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    • v.32 no.5
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    • pp.423-432
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    • 2019
  • In order to select potential plant resources as functional materials and natural antioxidants, we evaluated antioxidant activity and serotonin derivatives of safflower germplasm collected from five countries. N-(p-Coumaroyl) serotonin (CS) and N-feruloylserotonin (FS) were analyzed by using Ultra Performance Liquid Chromatography (UPLC). Total polyphenol content (TPC) was determined by Folin-Ciocalteu method and antioxidant activities were estimated by 2,2-diphenyl-1-picryl-hydrazil (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS), Ferric reducing antioxidant power (FRAP) and Reducing power (RP) assays. The TPC showed a range of 28.25 to $90.53{\mu}g$ gallic acid equivalent (GAE)/mg dried extract (DE). DPPH, ABTS, FRAP and RP assay were in the range of 18.76 to 93.98, 48.91 to 163.73, 3.80 to 132.29 and 26.32 to $80.08{\mu}g$ ascorbic acid equivalent (ASCE)/mg DE, respectively. Among the five countries, safflower seed collected from Iran had the highest levels of serotonin derivatives and antioxidant activities than other countries (p<0.05). CS showed high correlation with TPC, ABTS and DPPH (r=0.673,0.727,0.820), and FS showed high correlation with DPPH (r=0.740). Accessions IT321214 and IT321215 could be useful for development of new functional materials and could be used as a source of valuable natural antioxidant materials.

The Flora of Mt. Papyeong (Gyeonggi-do Prov.) in Western Area of DMZ, Korea (서부 DMZ 인근접경 지역의 파평산(경기도) 일대의 식물상)

  • NamGung, Ju;Yoon, Chang Young;Ha, Young-Ho;Kim, Joo-Hwan
    • Korean Journal of Plant Resources
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    • v.32 no.4
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    • pp.355-378
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    • 2019
  • We investigated the flora of vascular plants in Mt. Papyeong which is located in the northern part of Paju and the border of western DMZ through 13 surveys from April 2016 to August 2019. The vascular plants of Mt. Papyeong include 410 taxa; 361 species, 9 subspecies, 36 varieties, and 4 forms from 257 genera of 91 families. Among 410 taxa, we characterize endemic species, rare and endangered plants, floristic regional indicator plants, and climate change adapted plants in Korea. Specifically, there are 9 Korean endemic plants designated by Korea National Arboretum and 6 taxa by Korean Ministry of Environment. According to IUCN evaluation, there are 13 rare and endangered plants in Mt. Papyeong. On the other hand, 2 taxa were listed in a red list suggested by Korean Ministry of Environment. The floristic regional indicator plants include 46 taxa which are composed of 2 species of IV degree, 13 taxa of III degree, 11 taxa of II degree, and 20 taxa of I degree. Also, 27 naturalized plants were identified, and the percentage of Naturalized Index (NI) and Urbanization Index (UI) were 6.58%, and 8.43%, respectively. We hope that this investigation provide a valuable data for applying the further studies on the biodiversity and strategy of conservation of environment in the DMZ and Paju areas.

Risk Assessment of As, Cd, Cu and Pb in Different Rice Varieties Grown on the Contaminated Paddy Soil (중금속 오염 논토양에서 재배된 벼 품종간 위해성평가 비교)

  • Kim, Won-Il;Kim, Jin-Kyoung;Yoo, Ji-Hyock;Paik, Min-Kyoung;Park, Sang-Won;Kwon, Oh-Kyung;Hong, Moo-Ki;Yang, Jay-E;Kim, Jeong-Gyu
    • Korean Journal of Soil Science and Fertilizer
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    • v.42 no.1
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    • pp.53-57
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    • 2009
  • Heavy metal pollution may be one of the most serious challenges confront crop production and human health. Therefore, the selection of heavy metal tolerance cultivars which adapted to the contaminated fields will introduced a suitable solution for management this critical environmental risk. The objectives of this research is to assess human health risk using geochemical analyses and exposure assessment of heavy metals in rice cultivars. Risk for inhabitants in the closed mine area was comparatively assessed for As, Cd, Cu and Pb in 10 rice varieties as a major exposure pathway. The average daily dose (ADD) of each heavy metal was estimated by analyzing the exposure pathways to rice and soil. For the non-carcinogenic risk characterization, Hazard Quotient (HQ) and Hazard Index (HI) were calculated using toxicity indices provided by US-EPA IRIS. The different rice varieties revealed a wide range of HI values from 23.6 to 34.3, indicating that all rice varieties have a high potential toxic risk. The DA rice variety showed the lowest HI value while the TB rice variety the highest. The probabilities of cancer risk for As via rice consumption were varied with rice varieties ranging from 2.0E-03 to 3.5E-03 which exceeded the regulatory acceptable risk of 1 in 10,000 set by US-EPA. The DA rice variety also showed the lowest value while the TB rice variety gave the highest value. Our results indicate that risk assessment can be contribute to screen the pollution safe rice cultivars in paddy fields affected by the mining activity.

A reevaluation of the castles and palaces of Goryeo Gangdo (江都) using GIS (고려 강도(江都)의 성곽과 궁궐 재고찰)

  • KANG, Dongseok
    • Korean Journal of Heritage: History & Science
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    • v.54 no.4
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    • pp.174-191
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    • 2021
  • Gangdo (江都), a reproduction of Gaegyeong, was the capital of Goryeo for 39 years. However, due to the urgent wartime situation of the Mongol invasion and the geographical features of Ganghwa Island, the castle system and palace layout were somewhat different from those of Gaegyeong. Gangdo's castle can be understood as a triple castle system consisting of outer castle, middle castle, and inner castle. First, the outer castle was the first to be completed, and it was built at the forefront to prevent the Mongol army from invading in the first place. It is presumed that the section was between Huamdon and Hwadodon in the outer castle during the Joseon Dynasty. The middle castle can be seen as the present 'Middle Castle', a castle built of earth on the outskirts of the Ganghwa-mountain Castle. Considering the sophistication and robustness of the construction method confirmed in the archaeological research, this castle is thought to have been built under a meticulous plan. In other words, as the capital city, it was completed 'at last' as recorded in the Koryo History, after a long 18-year construction process to protect palaces, government offices, and private houses. The inner castle was a castle with the character of a palace. This corresponds to the Old Castle of Ganghwabu (江華府) during the Joseon Dynasty, and it almost coincided with the scale of the composition of Gaegyeong's palace castle. It was a complex functional space, featuring the integration of the palace and the imperial castle, where the main government offices and ancillary facilities, including the palace, were located. Based on the documentary record that these palaces were similar to Gaegyeong's palace, the palace map was overlapped with that of Gaegyeong. The central axis of the building from Seungpyeongmun (昇平門) to Seongyeongjeon (宣慶殿) coincided with Kim Sangyongsunjeol Monument in Ganghwa- Goryeo Palace. Therefore, it seems that the palace of Gangdo had the same basic structure as that of Gaegyeong. However, the inner palace and annexed buildings must have been arranged in consideration of the topographical conditions of Ganghwa, and this is estimated to be the Gunggol area in Gwancheong-ri.

Evaluation of applicability of linkage modeling using PHABSIM and SWAT (PHABSIM과 SWAT을 이용한 연계모델링 적용성 평가)

  • Kim, Yongwon;Byeon, Sangdon;Park, Jinseok;Woo, Soyoung;Kim, Seongjoon
    • Journal of Korea Water Resources Association
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    • v.54 no.10
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    • pp.819-833
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    • 2021
  • This study is to evaluate applicability of linkage modeling using PHABSIM (Physical Habitat Simulation System) and SWAT (Soil and Water Assessment Tool) and to estimate ecological flow for target fishes of Andong downstream (4,565.7 km2). The SWAT was established considering 2 multi purpose dam (ADD, IHD) and 1 streamflow gauging station (GD). The SWAT was calibrated and validated with 9 years (2012 ~ 2020) data of 1 stream (GD) and 2 multi-purpose dam (ADD, IHD). For streamflow and dam inflows (GD, ADD and IHD), R2, NSE and RMSE were 0.52 ~ 0.74, 0.48 ~ 0.71, and 0.92 ~ 2.51 mm/day respectively. As a result of flow duration analysis for 9 years (2012 ~ 2020) using calibrated streamflow, the average Q185 and Q275 were 36.5 m3/sec (-1.4%) and 23.8 m3/sec (0%) respectively compared with the observed flow duration and were applied to flow boundary condition of PHABSIM. The target stream was selected as the 410 m section where GD is located, and stream cross-section and hydraulic factors were constructed based on Nakdong River Basic Plan Report and HEC-RAS. The dominant species of the target stream was Zacco platypus and the sub-dominant species was Puntungia herzi Herzenstein, and the HSI (Habitat Suitability Index) of target species was collected through references research. As the result of PHABSIM water level and velocity simulation, error of Q185 and Q275 were analyzed -0.12 m, +0.00 m and +0.06 m/s, +0.09 m/s respectively. The average WUA (Weighted Usable Area) and ecological flow of Zacco platypus and Puntungia herzi Herzenstein were evaluated 76,817.0 m2/1000m, 20.0 m3/sec and 46,628.6 m2/1000m, 9.0 m3/sec. This results indicated Zacco platypus is more adaptable to target stream than Puntungia herzi Herzenstein.

Efficiency Analysis of Spiral Structured Twist Screen (식품분말 진동선별기 개선을 위한 구조물 효율 분석)

  • Park, In-soon;Na, En-soo;Jang, Dong-soon;Paek, Young-soo
    • Food Engineering Progress
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    • v.14 no.2
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    • pp.85-91
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    • 2010
  • In the food process, twist screen is widely used to divide particles on the basis of size. As screen equipped in the twist screen perfoms an important part in the particle size distribution mechanism, the contact area of screen and particles, retention time of particles on the screen, mesh and string thickness of screen and the flow pattern of particles on the screen are major points of the separation efficiency. To improve the separation efficiency, increase the retention time and control the flow pattern of particles, screen frame dam and spiral blockage are installed on the sieve of twist screen ${\emptyset}$ 1200 and ${\emptyset}$ 1500. Twist screen ${\emptyset}$ 1500 with frame dam treated similar separation capacity, 37% higher separation ratio and less non-separated particles of product output 1 than general twist screen. Twist screens with frame dam and spiral blockage showed less treatment capacity, three times higher division ratio and entire separation than general twist screen.

Improvement of State Ownership of Excavated Cultural Heritage System and Establishment of Policy Direction (발굴매장문화재 국가귀속제도의 정책 개선방안 연구)

  • Kim, Jong soo
    • Korean Journal of Heritage: History & Science
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    • v.49 no.1
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    • pp.22-43
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    • 2016
  • State Ownership of Excavated Cultural Heritage System was originated from the legislations concerning cultural objects during the Japanese colonial period (1910~1945) and was succeeded by the present Buried Cultural Properties Act enacted in 2011. Despite the importance of the system that completes the outcomes of excavations and determines the state-owned cultural properties, the foundation of national heritage, it has been limitedly regarded as administrative area and neglected by the academic scholars or policy researchers. Recently the traditional culture has drawn increasing domestic interest and awareness that the cultural heritage contributes to building cultural identity and vitalizing tourism has led to increasing the demand of a local government's role in management of the state-designated cultural heritage and even fighting for hegemony in securing the cultural objects between the central and local governments. Despite the continuing efforts for improving the selection process of cultural heritage and its management institution, establishment of an advanced objective system has been requested. This paper is intended to suggest the policy direction through demonstrating the problem and assignment caused in the process of implementing the Buried Cultural Properties Act and reviews the State Ownership of Excavated Cultural Heritage System from the legal point of view accordingly. First, I suggest improving the selection process of the state-owned cultural properties. Even though current law states that Administrator of Cultural Heritage Administration reviews the research reports and selects the possible candidates for the state-owned cultural properties almost all the cultural objects listed on the reports are practically selected. In this regard, two possible resolutions can be made; newly establishing a separate process for selecting the state-owned cultural properties after publishing the report or adding the selection process of the state-owned cultural properties during the heritage selection meeting. Either way should contribute to strengthening the impartiality and objectivity of the policy. My second suggestion is improving the operating system of the heritage selection meeting in which the cultural properties to be listed on the reports are determined. Given the present extensive assessment criteria, there is much room for certain experts' subjective opinions. Therefore, in order to enhance the fairness and credibility of the heritage selection meeting, specifying the assessment criteria and advance review of the expert list are necessary. Third, this paper suggests increasing the local government's role in management of the state-owned cultural heritage and diversifying the heritage management institution. Development of a local self-governing system has led to the increased demand for delegating the authority of the state-owned heritage management to the local governments. Along with this, the gradual improvements of public museum management raises the need for expanding the cultural benefits through increasing the local government's role in management of the state-owned heritage. Considering the fact that overall majority of the art collections housed at national or public museums is owned by the central government, developing a variety of heritage contents and vitalizing the heritage tourism are crucial. The true meaning and value of the state-owned cultural heritage hidden at the storage of a museum can be found when they are shared together with the public.

Estimation of Baseflow based on Master Recession Curves (MRCs) Considering Seasonality and Flow Condition (계절·유황특성을 고려한 주지하수감수곡선을 활용한 기저유출분리 평가)

  • Yang, Dongseok;Lee, Seoro;Lee, Gwanjae;Kim, Jonggun;Lim, Kyoung Jae;Kim, Ki-Sung
    • Journal of Wetlands Research
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    • v.21 no.1
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    • pp.34-42
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    • 2019
  • Baseflow which is one of the unmeasurable components of streamflow and slowly flows through underground is important for water resource management. Despite various separation methods from researches preceded, it is difficult to find a significant separation method for baseflow separation. This study applied the MRC method and developed the improved approach to separate baseflow from total streamflow hydrograph. Previous researchers utilized the whole streamflow data of study period at once to derive synthetic MRCs causing unreliable results. This study has been proceeded with total nine areas with gauging stations. Each three areas are selected from 3 domestic major watersheds. Tool for drawing MRC had been used to draw MRCs of each area. First, synthetic MRC for whole period and two other MRCs were drawn following two different criteria. Two criteria were set by different conditions, one is flow condition and the other is seasonality. The whole streamflow was classified according to seasonality and flow conditions, and MRCs had been drawn with a specialized program. The MRCs for flow conditions had low R2 and similar trend to recession segments. On the other hand, the seasonal MRCs were eligible for the baseflow separation that properly reflects the seasonal variability of baseflow. Comparing two methods of assuming MRC for baseflow separation, seasonal MRC was more effective for relieving overestimating tendency of synthetic MRC. Flow condition MRCs had a large distribution of the flow and this means accurate MRC could not be found. Baseflow separation using seasonal MRC is showing more reliability than the other one, however if certain technique added up to the flow condition MRC method to stabilize distribution of the streamflow, the flow conditions method could secure reliability as much as seasonal MRC method.

Effects of Vitamin E enhanced transgenic soybean cultivation on insect diversity (비타민 E 강화콩 재배가 곤충다양성에 미치는 영향)

  • Oh, Sung-Dug;Suh, SangJae;Park, Soo-Yun;Lee, Kijong;Sohn, Soo-In;Yun, Doh-Won;Chang, Ancheol
    • Korean Journal of Breeding Science
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    • v.49 no.3
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    • pp.129-140
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    • 2017
  • This study was carried out to develop of environmental risk assessments and the biosafety guide for Vitamin E enhanced transgenic soybean at LMO (Living Modified Organism) isolation field. In LMO quarantine area of National Institute of Agricultural Sciences, insect species diversities and population densities on vitamin E enhanced transgenic soybean and non-GM soybeans (Willams 82 and Seoritae) were investigated. A total of 17,717 individuals of 77 species from 8 orders were collected in LMO isolation field. In three type soybeans field, total of 5,250 individuals in Vitamin E enhanced transgenic soybean, 5,510 individuals in Willams 82, and 6,957 individuals in Seoritae were collected, respectively. There was no difference between the population densities of insect pests, natural enemies and other insects on Vitamin E enhanced transgenic soybean and Willams 82, while natural enemies density on Seoritae was higher than on Vitamin E enhanced transgenic soybean, but insect pests density on Vitamin E enhanced transgenic soybean was higher. These results provided the insects diversity for risk assessment survey of Vitamin E enhanced transgenic soybean and suggested that the guideline could be useful to detect LMO crops.

Temporal Variations of Sea Water Environment and Nutrients in the East Coast of Korea in 2013~2017: Sokcho, Jukbyeon and Gampo Coastal Areas (2013~2017년 동해 연안의 해양환경과 영양염의 시간적 변동 : 속초, 죽변, 감포 연안)

  • Kwon, Kee-Young;Shim, Jeong Hee;Shim, Jeong-Min
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.25 no.4
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    • pp.457-467
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    • 2019
  • To investigate the long-term variation characteristics of nutrients in the east coast of Korea, water temperature, salinity, dissolved oxygen, and nutrients were measured at three stations of Sokcho, Jukbyeon and Gampo coasts for five years from 2013 to 2017. For five years, the water temperature of the East Sea coast was in the range of $1.2{\sim}28.8^{\circ}C$, the salinity was in the range of 30.63~34.79 and the dissolved oxygen (DO) was in the range of 3.53~7.64 mL/L. Distribution and variation of the water environment factors in the study area were determined by the vertical stratification of water column and distribution of water temperature. The high DO concentration in Sokcho coast From 2015 to August 2016 is presumed to be the result of the southward inflow of North Korean Cold Water (NKCW). Concentrations of dissolved inorganic nitrogen (DIN, $NH_4-N+NO_2-N+NO_3-N$) ranged $0.11{\sim}24.19{\mu}M$, phosphate concentration ranged $0.01{\sim}1.75{\mu}M$, and silicate ranged $0.17{\sim}32.80{\mu}M$. The N:P ratio was in the range of 0.7~54.3 (mean 15.2) and the N:P slope was in the range of 11.67~13.75. The N:P ratios in this study were lower than the Redfield ratio (16), indicating that nitrate did act as a limiting factor in phytoplankton growth. The correlation ($R^2$) of total N:P ratio was as high as 0.95, indicating that the effect of the surrounding land or non-point sources was not significant. In conclusion, the spatial and temporal variation of nutrients in the east coast of Korea was determined by the vertical mixing of water mass with thermocline and mainly affected by physical factors such as influx of external water masses and coastal upwelling, and the influences from inflows from the land were minimal.