• Journal of Life Science
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• v.22 no.8
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• pp.1092-1098
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• 2012

This study was carried out to discover the skin wrinkle reducing components in the seeds of Arctium lappa. The isolation of a methylene chloride-soluble fraction of 70% ethanol extract from the seeds of Arctium lappa using a procollagen type-1 synthesis and MMP-1 activity resulted in the isolation and identification of four lignin compounds: arctiin, arctigenin, matairesinol, and diarctigenin. All structures were confirmed via NMR and MS spectroscopic data. To determine cell viability and procollagen type-1 synthesis, human dermal fibroblasts were treated with 10-100 ${\mu}M$. As a result, none of the four compounds showed cytotoxicity up to 50 ${\mu}M$. We also investigated their procollagen type-1 synthesis and MMP-1 inhibition activity and found that arctiin had the highest activity in terms of both procollagen synthesis and MMP-1 inhibition among all four compounds. Putting all the data together, we suggest that arctiin be used in cosmetics as an anti-wrinkle material.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2023.04a
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• pp.71-71
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• 2023

사람 장내세균은 그 개체가 100조 이상으로 알려져 있으며, 그 종류도 수 천 종에 이르러 건강에 지대한 영향을 미치고 있다. 이에 많은 연구를 통해 장내세균의 구성과 각종 질환과의 연관성이 밝혀지고 있으며, 장내세균 구성을 변화시켜 질환을 치료하려는 시도도 이어지고 있다. 본 연구에서는 천연식의약소재의 활성 검토를 위해 피부염 동물모델을 구축하고, 장내세균과의 상관관계를 정립하기 위해 실험을 수행하였다. 실험동물의 등 부위를 제모하고 DNFB로 피부염을 유발시켰으며, 유도 전에 대변을 채취하였다. 피부염의 유발을 확인하기 위해 비장의 무게를 측정하였다. Arctigenin과 dexamethasone을 실험군으로 설정하여 동물에 투여하여 그 효과를 검증함과 동시에 대변을 채취하여 장내세균의 구성을 T-RFLP법으로 분석하였다. 또한, 실험동물의 장내세균을 납품회사 별로 분석하여 향후 연구에 활용하고자 하였다. 그 결과, 장내세균의 구성이 피부염의 유발에 직접적으로 영향을 주는 것이 확인되지 않았다. 하지만, 투여 성분의 채내 동태 등에 대한 연구를 실시하여 약물대사를 포함한 장내세균과의 상호작용 연구를 통해 질환과 약물, 그리고 장내세균과의 관계를 정립할 수 있는 기반을 마련하였다.

• Preventive Nutrition and Food Science
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• v.11 no.4
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• pp.311-317
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• 2006

Phenolic compounds in safflower seeds were recently found to stimulate bone formation and increase plasma HDL cholesterol levels in estrogen deficient rats, and to inhibit melanin synthesis. Nine phenolic compounds: $N-feruloylserotonin-5-O-{\beta}-D-glucoside,\;8'-hydroxyarctigenin-4'-O-{\beta}-D-glucoside,\;luteolin-7-O-{\beta}-D-glucoside$, N-(p-coumaroyl)serotonin, N-feruloylserotonin, 8'-hydroxy arctigenin (HAG), luteolin (LT), $acacetin-7-O-{\beta}-D-glucuronide$ (ATG) and acacetin (AT), were quantified by HPLC in safflower (Carthamus tinctorius L.) seeds during growth and processing. During growth, levels of the nine phenolic compounds in the seeds increased progressively with increasing growth stages, reached a maximum on July 30 (42nd day after flowering), and then remained relatively constant. During the roasting process, levels of phenolic compounds, except HAG, LT and AT, generally decreased with increased roasting temperature and time, whereas those of HAG, LT and AT increased progressively with increased roasting temperature and time. During the steaming process, levels of other phenolic compounds except HAG and AT generally tended to increase with increased steaming time, whereas those of HAG and AT were scarcely changed. During the microwave treatment, quantitative changes of phenolic compounds were similar to the roasting process, although there were some differences in levels of phenolic compounds between two heat treatments. These results suggest that the steamed safflower seeds after harvesting on late July may be useful as potential dietary supplement source of phenolic compounds for prevention of several pathological disorders, such as atherosclerosis and osteoporosis and aging.

• Natural Product Sciences
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• v.17 no.2
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• pp.147-159
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• 2011

A high-performance liquid chromatography-electrospray ionization-tandem mass spectrometric method was developed to determine simultaneously eight marker constituents of Forsythiae fructus, and subsequently applied it to classify its two botanical origins. The marker compounds of Forsythia suspensa were phillyrin, pinoresinol, phillygenin, lariciresinol and forsythiaside; those of F.viridissima were arctiin, arctigenin and matairesinol. Separation of the eight analytes was achieved on a phenyl-hexyl column (150${\times}$2.0 mm i.d., 3 ${\mu}M$) using gradient elution with the mobile phase: (A) 10% acetonitrile in 0.5% acetic acid, (B) 40% aqueous acetonitrile. A few fragment ions specific to the types of lignans, among the product ions generated by collisonally induced dissociation (CID) of molecular ion clusters, such as [M-H]$^-$ or [M+OAc]$^-$ were used not only for fingerprinting analysis but for the quantification of each epimer by using multiple-reaction monitoring mode. It was shown good linearity ($r^2{\geq}$ 0.9998) over the wide range of all analytes; intra- and inter-day precisions (RSD, %) were within 9.14% and the accuracy ranged from 84.3 to 115.1%. The analytical results of 40 drug samples, combined with multivariate statistical analyses - principal component analysis (PCA) and hierarchical cluster analysis (HCA) - clearly demonstrated the classification of the test samples according to their botanical origins. This method would provide a practical strategy for assessing the authenticity or quality of the herbal drug.