• Food Science and Biotechnology
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• 제15권6호
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• pp.896-901
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• 2006

Patulin is a food mycotoxin which induces genotoxicity and acute intestinal disease in infants. Patulin mainly originates from fruit putrefactive moulds, especially in apples, which necessitates the maintenance of strong safety standards against patulin for fresh and processed apples. To investigate the patulin producing moulds in Korean apples, 16 morphological types of fungi were isolated from Korean apples and a patulin producing fungus was identified based on a sequence analysis of the region of internal transcribed spacers (ITS5-5.8S-ITS4 region, 505 base pair) and the 26 rRNA D1/D2 region (527 base pair). Morphological analyses were also performed. The isolated patulin producing fungus was found to a representative species of Penicillium crustosum. The maximal patulin production ability of the isolated fungus (P. crustosum) and the patulin producing standard strain (P. griseofulvum, ATCC 46037) in an SY broth medium were 0.32 and 2.46 mg/L, respectively.

• Journal of Biosystems Engineering
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• 제38권3호
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• pp.192-198
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• 2013

Purpose: Methods for non-destructive estimation of product quality have been reported in various industrial fields, but the application of ultrasonic techniques for the agricultural products of potatoes, pears, apples, watermelons, kiwis and tomatoes etc. have been rarely reported since the application of a contact-type ultrasonic transducer in agricultural products is very difficult. Therefore, this study sought to determine the firmness of apples using non-contact ultrasonic techniques. Methods: For this experiment, an ultrasonic experimental tester using a non-contact ultrasonic transducer was created, and a signal processing program was used to analyze the acquired ultrasonic reflected signal. Also, a universal testing machine was used to measure firmness parameters of the apples such as bioyield strength, a firmness factor, after the ultrasonic tests had been performed. Results: Six distance correction factors were calculated to obtain consistent values of ultrasonic properties regardless of the distance between the transducer and the surface of the subject. We developed prediction models of the bioyield strength using the distance correction factors. Conclusions: The optimum prediction model of the bioyield strength of apples using a non-contact ultrasonic technique was a multiple regression model ($R^2=0.9402$).

• 한국농업기계학회:학술대회논문집
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• 한국농업기계학회 2000년도 THE THIRD INTERNATIONAL CONFERENCE ON AGRICULTURAL MACHINERY ENGINEERING. V.II
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• pp.204-209
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• 2000

At harvest, the starch contents of apples, which were related to their maturity, were quantified by using image analysis. The stained area was measured by a computer vision system when an apple slice was stained with KI/I$_2$ solution. The stained area ratio of the stained area of the apple slice to its whole area, or the starch index, was defined as an indicator of starch content. When Tsugaru apples were manually sorted into immature, turning and mature groups, their starch indices were 0.374, 0.312 and 0.129, respectively. Meanwhile, the starch index had correlation to various physicochemical properties of Tsugaru apples. At the statistically significant level of 0.1 %, it was correlated with the pH value, bio-yield force, rupture force and color of intact and skin-removed apples. At the 1 ％ significant level, it had the correlation with the density and moisture content.

• Near Infrared Analysis
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• 제1권1호
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• pp.23-26
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• 2000

The objectives of this study were to examine the ability to predict soluble solid and firmness in intact apple based on the visible/near-infrared spectroscopic technique. Two cultivars of apples, Delicious and Gala, were handled, tested and analyzed. Reflectance spectra, Magness-Taylor (MT) Firmness, and soluble solids in apples were measured sequentially. Maximum and minimum diameters, height, and weight of apples were recorded before the MT firmness tests. Apple samples were divided in to a calibration set and a validation set. The method of partial least squares (PLS) analysis was used. a unique set of PLS loading vectors (factors) was development for soluble solid and firmness. The PLS model showed good relationship between predicted and measured soluble solids in intact apples in the wavelength range of 860∼1078 nm. However, the PLS analysis was not good enough to predict the apple firmness.

• Agricultural and Biosystems Engineering
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• 제1권1호
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• pp.38-42
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• 2000

Though the freshness for agricultural products is an important factor related to their quality management, this terminology is being used restrictedly because it is very subjective. In this study, a dimensionless index which had the span of the maximum of 1 through the minimum of 0 was proposed to describe freshness of the product with time-variant quality and was applied to Tsugaru and Fuji apples. First, the compressive properties having the linearity in their change regarding time elapsed after harvest were selected. For Tsugaru apple, bio-yield and rupture forces had high correlation with time while for Fuji, bio-yield and rupture deformations had high correlations. When the slope, or ratio of force to deformation, was considered, the effect of cultivar could be neglected. When the linearly time-variant compressive properties for Tsugaru and Fuji apples were involved in the freshness indices, they described well freshness of apples. Also, the freshness decay constant depicted a characteristic which related to freshness decay rate. Therefore, the freshness index can be utilized to manage the quality during storage and distribution of apples.

• 동아시아식생활학회지
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• 제14권4호
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• pp.343-347
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• 2004

Abundant amounts of unripe apples, which were picked out before maturation, were abolished every year fer wastes. Since unripen apples are known to be rich in functional components, the ultimate objective of this research was to utilize them for food resources. The concentrations of polyphenols in hot water extract were the lowest, those in 75% acetone extract the highest. Concentrations of polyphenols in 5/30 sample were 3.5 times higher than those in 6/30 sample regardless of solvents used for extraction. The degree of condensation of flavanolic tannins in the peel was lower than those of the other parts, and the hot water extract was more condensed than the acetone extract. The amount of total flavonoids in the peel and 5/30 samples were 2.7 and 5.0 times higher than those of the flesh and 6/30 samples, respectively.

• Applied Biological Chemistry
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• 제41권3호
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• pp.234-239
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• 1998

근적외 분광분석법으로 사과의 유리산 함량을 비파괴적으로 측정함에 있어서 사과 착즙액의 갈변에 의한 영향은 있었으나 실활처리 및 적정 알칼리액 농도는 측정 정확도에 영향을 주지 않았다. 수확기 사과의 유리산 함량은 수확 시기를 달리한 시료로 작성한 검량식을 사용하여 측정하였는데 중회귀분석 결과, 중상관계수(R)는 0.77이었고 측정오차(SEP)는 0.03%이었다. 저장 중인 사과의 유리산 함량은 저장하여 유리산 함량폭이 넓은 시료로 작성한 검량식을 사용하여 측정하였으며 그 결과 R은 0.90, SEP는 약 0.04%이었다. 근적외 분광분석법을 응용하여 사과 중의 유리산 함량을 정확히 정량하기는 다소 미흡하나 높은 산 함량치와 낮은 산 함량치를 가지는 사과로 분류하는 것은 가능한 것으로 판단되었다.

• Current Research on Agriculture and Life Sciences
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• 제31권4호
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• pp.272-279
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• 2013

사과 중 triazole계 살균제 flusilazole 및 myclobutanil의 생물학적 반감기와 출하전 잔류허용기준을 산출하기 위해 안전사용기준에 근거한 기준량과 3배량의 약제를 살포하고 그 잔류량을 조사하였다. 시험기간 중 두 농약의 잔류량은 각각의 MRL 이하로 나타났으며, 사과 중 생물학적 반감기는 flusilazole의 경우 기준량 처리구에서 6.7일, 3배량 처리구에서 6.2일로 나타났다. 반면에 myclobutanil의 반감기는 기준량 처리구에서 13.3일, 3배량 처리구에서 24.8일로 나타나 flusilazole보다 더 긴 반감기를 가지는 것으로 조사되었다. First order kinetics에 근거한 감소지수식을 이용하여 산출된 각 농약의 감소상수는 flusilazole에 대하여 0.0513, myclobutanil에 대하여 0.0244이었으며, 산출된 감소상수들을 이용하여 출하 전 잔류허용기준(PHRL)을 계산한 결과, 안전사용기준을 준수한 농약살포를 가정하였을 때 flusilazole은 수확 일주일 전 0.43 mg/kg, myclobutanil은 같은 시기 0.59 mg/kg 이하로 잔류하면 수확 시 잔류농도가 MRL 이하로 잔류할 것으로 예측된다.

• 한국유기농업학회지
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• 제7권2호
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• pp.1-16
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• 1999

In this study, questionnaire were made to environment-friendly producers of apples regarding farm management methods in low-input production method. utilizing organic materials, production and marketing of apples with lower agricultural chemical residues. Besides, five apples farms were visited to find out their management situation by employing the low-input production method in order to protect the environment as well as consumer's health. Those five low-input apple farms were scattered in Kyungbuk, Chungnam, and Chonnam Provinces, There were not many low-input apple farms across the nation from the beginning and, as a result, increasing the sample size was basically very difficult. Most of these farms were using 140hrs of labor per 10a, of which 30hrs in pre-season management, 25hrs in plowing and weed control, 15hrs in disease and pest control, 20hrs in harvest and marketing and 50hrs in miscellaneous activities. Relatively, pest control takes much time in that they would spray pesticides 7-10 times a season to control the apple disease, 9 times on the average. The average gross revenue of low-input apple farms was about \2,000,000/10a, and their average yield was 2,000kg/10a, which are 25% and 13% lower, respectively, than the ordinary apple production case. This means the low-input farmers are inefficient in marketing their products. On the other hand, their production costs were 20.4% higher than the ordinary apple farms on the average. Since the imported foreign fruits including apples must use various agricultural chemicals on their way to the export markets, the domestic low-input apples have competitive edge over them in therms of food safety. In order to improve the low-input apple industry, active cooperation is needed among the producers, government and researchers more than ever. Among other things, production cost reduction and quality improvement with lower chemical residues are part of the urgent matters to be done.

• 한국식품과학회지
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• 제31권5호
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• pp.1275-1282
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• 1999

무대 및 유대 Fuji 사과의 최적 CA 저장조건을 확립하기 위하여 저장조건에 따른 품질 특성들을 조사하였다 저장 동안 중량 유지율은 CA저장이 air저장보다 높았으나 CA조건간에는 큰 차이가 없었고, 동일한 저장조건에서 유대가 무대과실 보다 높았다. 과육경도 및 적정산도는 1% $O_2$ 조건이 3% $O_2$ 조건 보다 높게 유지하였으며 1% $O_2$에서는 3% $CO_2$가 1% $CO_2$보다 높게 유지하였다. 저장온도 $2^{\circ}C$ 보다는 $0^{\circ}C$에서 높은 과육경도와 적정산도를 유지하였으며, 적정산도의 감소는 유대가 무대과실 보다 빨랐다. 가용성 고형물의 변화는 무대와 유대과실 및 저장조건에 따른 유의적인 차이가 없었다. 과피색중 ${\Delta}E$값은 $2^{\circ}C$보다는 $0^{\circ}C$에서 3% $O_2$ 보다는 1% $O_2$조건에서 변화가 적었다. 내부갈변과는 1% $O_2$일 때 3% $CO_2$에서 저장 2개월 이내에 발생하였으며 실험년도 및 무대와 유대과실에 따른 뚜렸한 경향은 없었다. 관능검사 결과 모든 평가 항목에서 무대가 유대과실 보다 우수하게 평가되었다. 과실의 hardness, juiciness, acidity 및 overall acceptability는 1% $O_2$ 저장 과실이 3% $O_2$ 저장 과실보다 우수하였으며 1% $O_2$에서는 $0^{\circ}C$와 $2^{\circ}C$의 온도차, 1%와 3%의 $CO_2$에 따른 차이가 없었다. 객관적 품질특성 유지에 $O_2$의 $1%\;O_{2}+3%\;CO_{2}$가 가장 효과적이었으나 내부갈변과실이 발생할 가농성이 있어서 관능적 품질 평가에서 구별되지 않은 $1%\;O_{2}+1%\;CO_{2}$가 Fuji 사과의 CA저장에 적합한 저장조건으로 판단된다.